116 results about "Ammonium nitrogen" patented technology

The invention provides a soil ammonium nitrogen content hyperspectral prediction method based on an improved extreme learning machine, and relates to the technical field of hyperspectral prediction. The method comprises the following steps: firstly, collecting and treating a soil sample for soil spectral measurement and NH4 < + >-N content determination; measuring soil spectral reflectivity data; preprocessing the soil spectral reflectivity data to form a spectral reflectivity data set; then carrying out characteristic wave band selection by adopting a sequential forward selection algorithm; an improved butterfly optimization algorithm IBOA is adopted to optimize model parameters of an extreme learning machine ELM, and then a hyperspectral prediction model used for predicting the NH4 < + >-N content of the soil is constructed; and finally, the ELM model after parameter optimization is selected to construct a hyperspectral prediction model to predict the NH4 < + >-N content. The method not only provides theoretical and technical support for soil ammonium nitrogen content monitoring, but also provides important reference and guidance for soil nitrogen cycle research and soil management.

The invention discloses a sea-land interaction zone underground water pollution source tracing method based on multi-pollutant fingerprints, and the method comprises the steps: constructing a fingerprint feature library of eight key pollutants including cadmium, arsenic, nitrate nitrogen, ammonium nitrogen, phosphate, total petroleum hydrocarbon, sulfamethoxazole, pH value and the like; statistical characteristic models of four pollution sources of industrial emission, agricultural non-point sources, oil exploitation and aquaculture are established, Z-Score standardization and a similarity calculation method based on a standard normal distribution probability density function are adopted, comprehensive similarity evaluation is performed in combination with a risk weight distribution mechanism, a similarity threshold value of 0.7 is set to perform pollution source identification and sorting, and the pollution sources are identified and sorted according to the similarity threshold value. And correction is performed by considering environmental factors such as tide, season and geology of a sea-land interaction zone. The method can quickly and accurately recognize the groundwater pollution source of the sea-land interaction zone, the traceability accuracy rate reaches 80% or above, and scientific and technical support is provided for prevention and control of groundwater pollution of the sea-land interaction zone and formulation of an environmental protection policy.

The invention discloses an application method for realizing rice nitrogen fertilizer substitution by utilizing a photocatalytic nitrogen fixation material in the technical field of agricultural production. The application method comprises the following steps: S1, pretreating and proportioning a catalyst; s2, reaction container deployment and illumination optimization; s3, carrying out photocatalytic reaction and ammonium nitrogen generation; s4, synergistically applying base fertilizer and additional fertilizer; s5, monitoring and feeding back nitrogen utilization efficiency; the performance of the photocatalyst is improved by adopting a surface doping and composite modification technology, atmospheric nitrogen can be stably and efficiently converted into ammonium nitrogen which can be directly absorbed by rice under a complex environmental condition, and the nitrogen conversion rate and the nitrogen supply efficiency are improved; the AI algorithm is combined with the chlorophyll meter, the soil nitrogen sensor and the water ammonia nitrogen concentration data to predict and dynamically adjust the nitrogen demand of the rice in real time; a closed loop of nitrogen fixation-conversion-absorption-recovery is formed through a segmented application strategy of base fertilizer and topdressing in combination with physical and chemical synergism and a biochar improvement technology.

The invention relates to the technical field of agricultural fertilizers, in particular to an enzymolysis fish protein type amino acid-containing water-soluble fertilizer and a preparation method thereof, the fertilizer is prepared from 50-70% of enzymolysis fish protein stock solution and other components; the stock solution is prepared by pulping aquatic product processing leftovers, adjusting the pH value to 6.5-7.5, adding specific compound protease for enzymolysis, and performing enzyme deactivation and centrifugation; the macroelement liquid contains nitrate nitrogen and ammonium nitrogen (2: 1-4: 1), and the microelement chelating agent is prepared by reacting a stock solution small molecule peptide with a specific salt. The fish protein enzymolysis stock solution is prepared from aquatic product processing leftovers, high-proportion small molecule peptide and amino acid are obtained through specific enzymolysis and other processes, and by matching with high-content macroelements in a specific nitrogen form, nutrition can be rapidly supplemented, and crop photosynthesis and dry matter accumulation can be improved; and moreover, all the components cooperate to regulate the growth of the crops and improve the stress resistance, and the surfactant also improves the utilization rate of the fertilizer and reduces waste.

The invention provides an astragalus mongholicus residue biochar-loaded microbial inoculant and a preparation method and application thereof.The preparation method comprises the steps that an Enterobacter mori XJ 10 inoculant is cultured firstly, then modified biochar is obtained through high-temperature pyrolysis of astragalus mongholicus residues and modification of industrial brown sugar, the modified biochar and the astragalus mongholicus residues are mixed according to the proportion, oscillated adsorption and vacuum drying are conducted, and the biochar-loaded functional inoculant is obtained. When the Cd-Pb composite contaminated soil is remediated by applying the microbial inoculum and the king grass cooperatively, compared with a contaminated control group (CK-2), the plant height, the stem diameter and the biomass of the king grass are increased by about 119.3%, 116.7% and 53.8% respectively, and the biomass is 9.8% higher than that of original soil (CK-1); ammonium nitrogen, nitrate nitrogen, available phosphorus and quick-acting potassium in the soil are increased by 255%, 892.6%, 203.5% and 301.6% respectively; and the Cd removal rate and the Pb removal rate are respectively increased by about 24.5% and 24.3%. Resource utilization of the radix astragali residues is achieved, a'charcoal-fungicide-king grass' synergistic system breaks through the limitation of a single remediation technology, pollution remediation and soil fertility improvement are both considered, and the method has the advantages of being low in cost, efficient and sustainable.

The invention discloses a photocatalytic nitrogen fertilizer preparation and application integrated device in the technical field, the photocatalytic nitrogen fertilizer preparation and application integrated device comprises a floating support, the floating support is arranged in a paddy field, the upper part of the floating support is fixedly provided with a nitrogen fertilizer preparation mechanism, and the nitrogen fertilizer preparation mechanism comprises a reaction tank; the device uses solar energy as the only energy to drive the air pump, and does not depend on fossil energy; in combination with reaction period control, the concentration of ammonium nitrogen in the field water can be stably maintained at a proper concentration; ammonium nitrogen is dissolved in water and directly enters crop rhizosphere to be absorbed; the lifting ring is pushed and extruded through the reset spring, the aeration nozzle is used for aeration, the gradient photocatalytic material can dissolve ammonium nitrogen generated by nitrogen in air in water under photoreaction, under the action of the reset spring, the lifting ring can achieve lifting adjustment, the position can be conveniently adjusted through air pressure, and the gradient photocatalytic material can be recycled. And the turbine blades can rotate by virtue of the aerated air flow, so that a solution in the reaction tank is stirred, and the sufficiency of ammonium nitrogen dissolved in water is conveniently improved.

The invention relates to a method for determining 15N isotope abundance of ammonium salt by using a high-resolution liquid chromatograph-mass spectrometer, which comprises the following steps: converting 15N-labeled ammonium nitrogen in a 15N-labeled ammonium salt sample into a 15N-labeled organic matter by using a derivatization reagent, and detecting the 15N in-situ abundance by using the high-resolution liquid chromatograph-mass spectrometer. Compared with the prior art, the method can be used for in-situ abundance detection of the 15N labeled ammonium salt inorganic sample, and has the advantages of simplicity, convenience, high applicability, accuracy and the like.

This invention discloses a slow-release fertilizer suitable for lateritic soils in Hainan, comprising a fertilizer core and a coating layer; the fertilizer core comprises the following raw materials in parts by weight: 10-20 parts isobutylene diurea, 1-3 parts 3,4-dimethyl-1H-pyrazole dihydrogen phosphate, 1-3 parts dicyandiamide DCD, 20-30 parts humic acid, and 30-50 parts organic fertilizer; the coating layer comprises the following raw materials in parts by weight: 5-10 parts natural nanotube halloysite, 3-5 parts bentonite, 5-10 parts cyclodextrin, 1-3 parts polyglutamic acid, 1-3 parts wood vinegar, and 5-10 parts polyacrylamide. The isobutylene diurea, humic acid, and organic fertilizer of this invention can effectively replenish organic matter and nitrogen in iron-aluminate soil, maintain soil nitrogen levels, and effectively control the conversion of ammonium nitrogen to nitrate nitrogen in lateritic soil, thereby reducing nitrogen loss and achieving a stable supply of nitrogen. In addition, polyglutamic acid and polyacrylamide have strong water retention capacity, and the fertilizer core is coated with a coating layer to achieve slow release and water retention.

The invention belongs to the technical field of pathogenic bacterium wastewater treatment, and particularly relates to a method capable of effectively and synergistically removing ammonia nitrogen and pathogenic bacteria in wastewater. According to the method, in a water body in which ammonia nitrogen and pathogenic bacteria coexist, the two pollutants are removed at the same time by utilizing the oxidation-sterilization synergistic effect of Fe (VI) / PAA. Pollutant ammonium nitrogen in the oxidation system serves as a resource to participate in removal of pathogenic bacteria, the existence of ammonium nitrogen promotes generation of intermediate oxidation products Fe (IV) and Fe (V) of potassium ferrate, then the oxidation inactivation capacity of the system is enhanced, the inactivation time of the pathogenic bacteria in wastewater is effectively shortened, meanwhile, nitrogen is converted into nitrogen, nitrate and the like, the removal rate of NH4 < + > within 30 min reaches 98.6%, and the removal rate of potassium ferrate reaches 98.6%. The total nitrogen removal rate reaches 97.2%, pathogenic bacteria inactivation and ammonium salt removal are carried out at the same time, and a new thought and technology are provided for removing pathogenic bacteria and ammonium salt in water treatment.

This invention belongs to the field of fertilizer additive technology, specifically relating to a Cu-containing additive. Ⅱ The coordination polymers, preparation methods, and applications are described. The preparation methods include: synthesizing N,N'-bis(3-pyridinecarboxamide)-1,2-ethane using nicotinic acid and 1,2-ethylenediamine as raw materials; and synthesizing Cu-containing polymers from N,N'-bis(3-pyridinecarboxamide)-1,2-ethane, CuCl2·2H2O, and 1,4-cyclohexane using a hydrothermal method. Ⅱ The coordination polymer [Cu(1,4-chdc)(3-dpye)]·3H2O is a blue crystal. The product prepared in this invention is used as a fertilizer additive in urea to reduce the conversion of urea N to ammonium nitrogen and ammonium nitrogen to nitrate nitrogen during urea application, thereby reducing the volatilization of NH3 into the air after urea application and the subsequent volatilization of N2O.

The invention relates to the technical field of agricultural resources and environment, in particular to a fertilization method for reducing nitrogen loss of an apple orchard. The apple branch biochar is prepared from the apple orchard pruning waste, and resource utilization of the waste is achieved. Through the synergistic effect of the apple branch charcoal, dicyandiamide and the reduced nitrogen fertilizer, the loss of nitrate nitrogen in soil is obviously slowed down, the conversion of ammonium nitrogen is inhibited, and a nitrogen cycle functional microflora is regulated, so that the application amount of the nitrogen fertilizer is reduced, the utilization rate of the nitrogen fertilizer is improved, and the environmental pollution is reduced; the growth of apple plants can be obviously promoted, the root configuration is improved, and the root activity and the nitrate reductase activity are improved.

The invention discloses an inhibitor for preventing excessive growth of towel gourd strips and application of the inhibitor, and relates to the technical field of planting. The inhibitor comprises the following components: phosphatidic acid, nitrifying bacteria and lignin, wherein the mass ratio of phosphatidic acid to nitrifying bacteria to lignin is (15-20): (16-20): (8-10). The phosphatidic acid and the nitrifying bacteria are introduced, so that the ammonium nitrogen absorption degree of towel gourd roots can be reduced, excessive growth of towel gourds is effectively prevented, the adverse effects of the inhibitor on the growth and quality of the towel gourds are avoided, and the method is low in cost, simple in process and easy to popularize and use.

The application discloses a method for promoting early flowering of strawberries, and belongs to the technical field of cultivation. The method uses exogenous H2O2 to achieve the effects of promoting flower bud differentiation and promoting roots. First, 10 days before planting of the strawberries, H2O2 solution is sprayed on the whole plant, with a concentration of 5 mmol / L, and the spraying is continuously carried out for 10 days, with the leaf buds being mainly sprayed; then, after the seedlings are successfully recovered, one piece of new root-promoting prescription (brown sugar+H2O2+paenibacillus polymyxa) is placed beside each seedling; finally, after the seedlings are recovered, 15 mmol / L ammonium nitrogen fertilizer is flushed and applied for 2-3 times. The method can effectively promote early flower bud differentiation of the strawberries at low cost, and can achieve the effects of root protection and root promotion.

The present application relates to the technical field of aquatic environment, and in particular to a lactic acid bacteria, a separation and screening method and application, wherein the lactic acid bacteria is Lacticaseibacillus casei FC-5 with a preservation number of CCTCC NO: M 2026260, and the present application further provides a method for application of the lactic acid bacteria and chlorella in bio-floc technology, and solves the problem of serious threat of accumulation of nitrite nitrogen to aquatic animals in aquatic environment, and also solves the problem of serious threat of accumulation of ammonium nitrogen to aquatic animals.

The invention aims to provide a method for increasing soil fertility and measuring ammonium nitrogen of the soil. The method for increasing the soil fertility comprises the step of adding the microbial agent into the soil to prepare the composite microbial agent for repairing the saline-alkali soil and improving the soil fertility, and the composite microbial agent adapts to various types of soil, so that organic matters of the soil are increased, available nitrogen, phosphorus and rapidly available potassium are remarkably increased, the salinity and alkalinity are effectively reduced, and the soil fertility is improved. Meanwhile, the invention provides a soil ammonium nitrogen measuring method which is efficient, visual, low in cost and capable of measuring on site.

The present invention proposes an automatic sampling system for leaching solutions at different soil depths in farmland, comprising a soil leaching solution sampling unit, a soil leaching solution delivery unit, and a soil leaching solution sampling controller. The soil leaching solution sampling unit and the soil leaching solution delivery unit are both connected to the soil leaching solution sampling controller, and the soil leaching solution sampling unit is connected to the soil leaching solution delivery unit. The soil leaching solution sampling controller monitors the soil moisture content of the leaching solution sampling unit in real time, automatically activates the automatic sampling system for leaching solutions at different soil depths under set soil moisture parameters, delivers the soil leaching solution to a designated location via the soil leaching solution delivery unit, and monitors the chemical composition of the soil leaching solution in real time. The present invention can automatically extract leaching solutions from different soil profiles in real time based on soil moisture content, and automatically completes leaching solution sampling and delivery, laying the foundation for obtaining nitrate nitrogen, ammonium nitrogen, and other chemical components in leaching solutions at different soil depths.

The present application relates to a kind of by chrysophyte fermentation-light coupling high-efficiency production of fucoxanthin method, comprising: chrysophyte is inoculated into fermenter and is carried out heterotrophic culture, while the algal liquid is continuously or intermittently lighted;Culture medium includes basic medium and feed medium, C / N is 4:1-40:1, nitrogen source uses organic nitrogen source or inorganic ammonium nitrogen;The glucose concentration in basic medium is 5-15g / L, temperature is 25-32℃, dissolved oxygen is controlled at 5-80%, pH is 4-8, light intensity 5-600 μmol m ‑2 s ‑1 ;Glucose concentration is monitored in time during the cultivation process, when glucose concentration is reduced to 0.5-3g / L, using peristaltic pump to flow feed medium is added to flow culture, control the glucose concentration in whole flow culture process in 1-10g / L;After 100h of cultivation, every 1-5h is sampled to detect the biomass dry weight contained in the culture medium in fermenter, when the detection value is unchanged or begins to drop, fermentation is finished, harvest, and the harvested product is used for extracting fucoxanthin.The present application establishes the feasibility basis of fermentation-light coupling chrysophyte cultivation to scale production of fucoxanthin.

The application belongs to the technical field of biochar application, and provides a biochar arid region agricultural application method, which comprises application of biochar to total nitrogen content of soil in monoculture and intercropping planting modes; the application utilizes physical characteristics such as water retention of biochar to improve soil nutrient availability and crop nutrient utilization efficiency; biochar in different planting modes significantly reduces total nitrogen content of soil in a 60-160cm soil layer, and the better the adsorption effect of biochar on total nitrogen content of soil, the more conducive to nitrogen requirement of crops in the growth and development process; intercropping planting mode significantly improves nitrate nitrogen content of soil in a 0-160cm soil layer, and addition of biochar can obviously reduce leaching risk of nitrate nitrogen, can reduce leaching loss amount of water-soluble ions such as nitrate nitrogen, and delay migration and conversion time; monoculture planting mode significantly improves ammonium nitrogen content of soil in a 0-160cm soil layer, and addition of biochar can obviously reduce loss risk of ammonium nitrogen, and is conducive to growth and development of crops.

The invention belongs to the technical field of fertilizer additives, and particularly relates to a CuII-containing coordination polymer and a preparation method and application thereof.The preparation method comprises the steps that N, N '-bis (3-pyridinecarboxamide)-1, 2-ethane is synthesized with nicotinic acid and 1, 2-ethylenediamine as raw materials; the preparation method comprises the following steps: synthesizing a coordination polymer [Cu (1, 4-chdc) (3-dpye)]. 3H2O containing Cu II from N, N '-bis (3-pyridinecarboxamide)-1, 2-ethane, CuCl2. 2H2O and 1, 4-cycloadipic acid by using a hydrothermal method, so as to obtain a blue crystal. The product prepared by the invention is applied to urea as a fertilizer additive, so that the conversion of urea N to ammonium nitrogen and the conversion of ammonium nitrogen to nitrate nitrogen in the urea application process are reduced, and the volatilization of NH3 in the air and the subsequent volatilization of N2O after the urea is applied are reduced.

The application relates to the field of agricultural solid waste resource utilization, and discloses a method for regulating and controlling nitrogen fixation and emission reduction of tobacco straw and sheep manure compost by tobacco straw biochar. The compost composition comprises 100 parts by weight of a basic fermentation mixture composed of tobacco straw aggregate and sheep manure, and 5 to 20 parts by weight of homologous tobacco straw biochar. The regulating and controlling steps include: mixing 30% to 35% of the total amount of biochar when the compost is built, neutralizing the initial free acid in situ and driving temperature rise; real-time monitoring of the core temperature of the compost, when the core temperature reaches above 60 DEG C and the temperature shows a continuous 6-hour cooling trend, the remaining 65% to 70% of the biochar is subjected to micro-spraying pre-wetting treatment, and the biochar is targetedly added by deep injection of a high-temperature reaction zone by using a rotary tillage injection machine. The application uses the segmented adding process of homologous biochar, overcomes the pore blockage defect caused by one-time addition, accurately fixes ammonium nitrogen in the gas burst period, avoids the heat shock and throwing dispersion of the targeted addition, and improves the composting quality of the organic fertilizer.

The invention belongs to the technical field of agricultural chemistry, and particularly relates to a fertilizer, a preparation method, a use method and application. The invention provides the application of the ammonium nitrogen fertilizer in improving the salt and alkali resistance of inositol, the inositol can participate in the formation of ascorbic acid, remove excess free oxygen and relieve plant salt damage stress, and the inositol derivative PI4P improves the activity of plasma membrane ATP enzyme, strengthens an SOS1 signal channel and improves proton potential energy for excretion of sodium ions. Ammonium ions of the ammonium nitrogen fertilizer can strengthen the activity of SOS2, so that SOS1 salt elimination is regulated and controlled, plant rhizosphere acidification is promoted, higher proton potential energy is improved for sodium ion excretion, salt damage is relieved, and ammonium nitrogen can strengthen the salt-resistant effect of inositol. The invention also provides a fertilizer which takes inositol and ammonium nitrogen as core active components and is suitable for stress-resistant growth-promoting cultivation of crops in a salinized soil environment.

An aeration tank and a sewage treatment system are characterized in that an anoxic regulation and control mechanism and an aerobic regulation and control mechanism are arranged in an aeration tank body, the anoxic regulation and control mechanism can create an anoxic environment around the anoxic regulation and control mechanism so that the area becomes an anoxic section, and the aerobic regulation and control mechanism creates an aerobic environment around the aerobic regulation and control mechanism so that the area forms an aerobic section; in the process that sewage flows along the channel, the sewage sequentially passes through the anoxic zone and the aerobic zone, in the anoxic zone, most nitrate radicals and nitrite radicals in the sewage are reduced into nitrogen by denitrifying bacteria in activated sludge, and nitrogen elements in the sewage are removed; in the aerobic section, most ammonium nitrogen in the sewage is converted into nitrate and nitrite by nitrifying bacteria in the activated sludge, and nitrogen in the sewage is further converted and removed; the denitrification effect of the anoxic section and the nitrification effect of the aerobic section are synergistically carried out, so that nitrogen elements in sewage can be more effectively removed, the denitrification efficiency is greatly improved, up-to-standard discharge of the sewage is ensured, and eutrophication pollution to a water body is reduced.

This invention belongs to the field of crop breeding, specifically relating to an NtLHT1 gene and its application in tobacco's resistance to low nitrogen stress. It involves artificially intervening in the mutation of specific nucleotide sequence fragments in the NtLHT1 gene to obtain two mutated nucleotide sequence fragments, resulting in a mutant NtLHT1 gene, and the application of the mutant NtLHT1 gene in improving tobacco's resistance to low nitrogen stress. The NtLHT1-edited tobacco plants of this invention exhibit enhanced absorption of nitrate and ammonium nitrogen during their vigorous growth period, increased activity of nitrate reductase and glutamine synthase, increased expression of nitrogen transporter genes, and improved nitrogen utilization efficiency. This invention increases the activity of nitrate reductase and glutamine synthase, thereby improving tobacco's resistance to low nitrogen stress and providing a genetic resource and material basis for the development of nitrogen utilization-related genes and strains in tobacco.

The invention discloses a metal ion chelate for inhibiting urease and / or nitration, the general molecular formula of the structure is MDPA, M is a metal ion, and DPA is pyridine-2, 6-dicarboxylic acid; the invention also discloses a preparation method of the metal ion chelate for inhibiting urease and / or nitration. According to the invention, pyridine-2, 6-dicarboxylic acid is adopted as a ligand, and a polydentate coordination method is adopted for preparation, so that the compound is excellent in structural stability, simple in preparation process, outstanding in repeatability and operability, remarkable and efficient in urease inhibition performance, and capable of slowing down the degradation speed of urea and reducing the loss amount of nitrogen in soil; the compound fertilizer has the advantages that the nitrogen utilization rate is increased, favorable conditions are created for crop growth, the compound fertilizer shows an inhibition effect on nitrobacteria, the conversion process from ammonium nitrogen to nitrate nitrogen in soil can be effectively reduced, the nitrogen emission in the fertilizer release process is reduced, the compound fertilizer is environment-friendly, and the absorption efficiency of fertilizer nutrients can be improved.

The invention discloses a preparation method of a NiII-containing coordination polymer and research on the inhibition effect of the NiII-containing coordination polymer on soil urease and nitrifying bacteria, and the preparation method comprises the following steps: synthesizing N, N-dimethylformamide by taking pyridine-3-carboxylic acid and 1, 2-ethylenediamine as raw materials; the invention discloses a novel coordination polymer [Ni (1, 4-chdc) (3-dpy) (H2O) 2], which is prepared by the following steps of: synthesizing N, N '-bis (3-pyridinecarboxamide)-1, 2-ethane by using NiCl2. 2H2O, the synthesized N, N'-bis (3-pyridinecarboxamide)-1, 2-ethane and 1, 4-cycloadipic acid through a hydrothermal method, and researching the influence of the novel coordination polymer [Ni (1, 4-chdc) (3-dpy) (H2O) 2] as an inhibitor on soil urease and nitrifying bacteria by analyzing the crystal structure of the novel coordination polymer [Ni (1, 4-chdc) (3-dpy) (H2O) 2]. The research on the compound as an inhibitor reduces the conversion of urea from urea N to ammonium nitrogen and the conversion of ammonium nitrogen to nitrate nitrogen, improves the utilization rate of the fertilizer urea and the stability of the urea, reduces environmental pollution and improves the grain yield. Compared with a traditional inhibitor, the novel NiII coordination polymer serving as a urease and nitrification inhibitor has the advantages of longer half-life period, small toxic and side effects, low preparation cost and more comprehensive functions, the utilization rate of nitrogen fertilizer urea can be greatly increased, the grain yield is increased, and the environmental pollution is reduced.

The application discloses application of a porin PorA in improving the ammonium nitrogen utilization capacity of a methanotrophic bacterium. The porin PorA has an amino acid sequence as shown in SEQ ID NO. 1. The coding gene porA of the porin PorA has a nucleotide sequence as shown in SEQ ID NO. 2. The application of the porin PorA in improving the ammonium nitrogen utilization capacity of Methylotuvimicrobium buryatense. Inactivation of the porin PorA can make the methanotrophic bacterium Methylotuvimicrobium buryatense grow by utilizing ammonium nitrogen, reduce the cost of fermenting the bacterium, and is of great significance for industrial application of the bacterium.

The invention provides a method for detecting organic material nitrogen mineralization characteristics and application thereof, and belongs to the technical field of organic fertilizer mineralization detection. The method for detecting the mineralization characteristic of the nitrogen in the organic material comprises the following steps: carrying out 15N labeling on the organic material, mixing the organic material subjected to 15N labeling with soil, collecting a mixed sample, determining the content of nitrate nitrogen, the content of ammonium nitrogen, the abundance of 15N in nitrate nitrogen and the abundance of 15N in ammonium nitrogen, and then calculating the net mineralization rate of the nitrogen in the organic material. The method for detecting the mineralization characteristics of the nitrogen in the organic material can detect the mineralization process of the organic material after the organic material is added into soil, and has the advantages of high detection sensitivity and high accuracy. By measuring the nitrogen mineralization rate of the organic materials (such as corn straw and chicken manure organic fertilizer), the nitrogen release dynamic state of the organic materials in the soil can be quantified, and the optimal fertilization time and dosage are matched for the growth cycle of crops.

The invention provides a notopterygium root complex microbial inoculant with excellent shape and high quality and a use method thereof, and belongs to the technical field of plant cultivation. The invention provides a complex microbial inoculant for inhibiting the formation of lateral roots of notopterygium seedlings, which comprises Witzia ciliaris and Brevibacillus laterosporus. The complex microbial inoculant is mainly used for reducing the content of 1-aminocyclopropane-1-carboxylic acid (on the premise of ethylene synthesis) and the content of ICA (Indoleacetic Acid) (auxin) by increasing the content of salicylic acid; the activity of peroxidase and glutamine synthetase and the content of total phenols in plants are improved; the contents of soil urease, polyphenol oxidase and alkali-hydrolyzable nitrogen are increased, and the contents of rapidly available potassium and total phenolic acid are reduced; and ammonium nitrogen is promoted to be converted into nitrate nitrogen, so that the generation of lateral roots of the notopterygium roots is inhibited, the nitrogen content of seedlings of the notopterygium roots is increased, the disease resistance is improved, and the breeding of excellent-shape and high-quality seedlings is promoted.

This application relates to a method and its application for reducing nitrate nitrogen to ammonia nitrogen in wastewater using a dual-bacterial inoculant. The method involves separately expanding, washing, and resuspending electron-donating bacteria and dissimilatory nitrate-reducing ammonium-producing bacteria, then mixing them in a co-culture medium to form a dual-bacterial inoculant; introducing the co-culture medium containing the dual-bacterial inoculant into the wastewater to be treated, and reducing nitrate nitrogen to ammonia nitrogen in the wastewater under anaerobic conditions. This method can reduce nitrate nitrogen in wastewater into a high-energy-density energy storage substance, and can also alleviate the problems of low utilization rate of ammonium nitrogen fertilizer and non-point source pollution, which is of great value for nitrogen pollution control and green sustainable agricultural development.