94 results about "Total petroleum hydrocarbon" patented technology

The invention relates to a petroleum degradation bacterium in oil-containing sludge. The degradation bacterium is preserved in the Chinese Academy of Sciences of the China General Microbiological Culture Collection Center CGMCC in #3, No.1 Courtyard, Western Beichen Road, Chaoyang District, Beijing, the preservation serial number is CGMCC8647, and the class number is Corynebacteriumglutamicum. The petroleum degradation bacterium (RSI) is separated from the oil-containing sludge in the Bohai Oilfield, the preservation serial number is CGMCC8647, the experiment shows that the RSI is relatively high in degradation rate for saturated hydrocarbon, that is, as high as 20.74 percent, the degradation rate of the bacterium for total petroleum hydrocarbon (TPH) in the oil-containing sludge within 30 days is 39.69 percent, and the optimal degradation conditions of the bacterium for petroleum are that the temperature is 37 DEG C, the salinity is 0.5-5 percent, and the pH value is 6-8.

The invention provides a biochemical process for in-situ remediation of black and odorous sediments polluted by organic matters. The biochemical process comprises the following steps: S1, adding sodium acetate into the sediments; S2, adding calcium nitrate into the sediments. The biochemical process has the beneficial effects that firstly, sodium acetate are added into the sediments, so that available carbon sources are provided for microorganisms in the sediments, bio-enzymes capable of degrading PAHs (polycyclic aromatic hydrocarbons) and TPH (total petroleum hydrocarbons) are induced while a large quantity of the microorganisms reproduce and grow, and PAHs and TPH are quickly degraded and removed by adopting a methane production process and a sulfate reduction process under the enzymatic reaction; secondly, calcium nitrate is added into the sediments while the effect of continuously degrading low-molecular-weight organic matters and a relatively good removal effect on the low-molecular-weight organic matters are achieved; the purposes of degrading organic matters with different molecular weight and removing difficultly degraded organic matters from the sediments are achieved through the synergistic effect of sodium acetate and calcium nitrate.

The invention relates to the technical field of determination of content of an organic mixture in soil and specifically discloses a detection method for total petroleum hydrocarbon in contaminated soil. The detection method comprises the following steps: 1) extracting: mixing to-be-detected soil with an extracting reagent, performing ultrasonic extraction, centrifuging and taking supernate as a to-be-detected sample solution, wherein petroleum ether is taken as the extracting reagent, the ratio of soil to liquid is 1:4, the ultrasonic extraction is performed for two times, the ultrasonic extraction duration is 20min each time and the ultrasonic extraction power is 100W; 2) determining: using a microplate reader for determining the absorbance value of the to-be-detected sample solution under the detection wavelength and utilizing an external standard method to determine the content of total petroleum hydrocarbon in the to-be-detected sample solution, wherein the detection wavelength is 304nm. The detection method provided by the invention has the advantages of less extracting agent dosage, low pollution, high detection speed, high accuracy, less determining solution dosage and suitability for quantitative determination of a trace of petroleum pollution environmental samples.

The present invention relates to a petroleum-degrading bacterium in oily sludge. The degrading bacterium is preserved in CGMCC, General Microorganism Center of China Microbial Strain Preservation Management Committee, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Chinese Academy of Sciences, Deposit number: CGMCC8647, classification number is Corynebacterium glutamicum. This application isolates oil-degrading bacteria (RS1) from oily sludge in Bohai Oilfield, and its preservation number is CGMCC8647. It has been proved by experiments that RS1 has a high degradation rate of saturated hydrocarbons, reaching 20.74%. The degradation rate of total petroleum hydrocarbons (TPH) was 39.69%. The optimal conditions for the bacteria to degrade petroleum were 37°C, salinity 0.5%-5%, and pH6-8.