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Degrading bacteria JN2 for petroleum hydrocarbons in oily sludge and its application

A technology of JN2 and degrading bacteria, applied in the field of bioremediation, can solve the problems of refractory degradation and complex components of oily sludge, and achieve the effects of enhancing biological activity, optimizing soil microbial system, and promoting degradation

Active Publication Date: 2018-06-01
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex composition of oily sludge, which is difficult to degrade, there are relatively few researches on microbial degradation and remediation of oily sludge at home and abroad.

Method used

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  • Degrading bacteria JN2 for petroleum hydrocarbons in oily sludge and its application
  • Degrading bacteria JN2 for petroleum hydrocarbons in oily sludge and its application
  • Degrading bacteria JN2 for petroleum hydrocarbons in oily sludge and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Degradation and repair test of oily sludge in Liaohe Oilfield

[0052] Oily sludge from Liaohe Oilfield was taken, air-dried and ground, passed through a 1mm sieve, and set aside. Pick a single colony in sterilized LB liquid medium, seal it with a gas-permeable sealing film, place the Erlenmeyer flask in a shaker at 120 rpm, and activate at 30°C for 24 hours. Weigh 40g of the above air-dried, ground and sieved oily sludge, add OD600=1 Rheinheimera sp.JN2 activated bacterial solution according to 10% inoculation amount, adjust the water-soil ratio to 2:5 with distilled water, and seal it with a breathable sealing film. All Erlenmeyer flasks were placed in a constant temperature incubator at 30°C for static cultivation, samples were taken every 5 days, and the content of total petroleum hydrocarbons in the oily sludge was measured with an infrared spectrophotometer. The degradation curve is shown in the attached image 3 shown. It can be seen from the figure ...

Embodiment 2

[0056] Example 2: Degradation effect of Rheinheimera sp.JN2 on petroleum hydrocarbons in oily sludge at different pH values

[0057] Oily sludge from Liaohe Oilfield was taken, air-dried, ground, and passed through a 1mm sieve. Weigh 40 g of the above oily sludge in a conical flask, and use dilute H 2 SO 4 and NaOH to adjust the pH of the soil to be 1, 3, 5, 7, 9, 11 respectively, add the activated bacterial solution of Rheinheimera sp.JN2 with OD600=1 according to 10% inoculum size, and adjust the water-soil ratio to 2:5 with distilled water, Seal with breathable parafilm. All the Erlenmeyer flasks were placed in a constant temperature incubator at 30°C for static cultivation and samples were taken after 25 days, and the total petroleum hydrocarbon content in the oily sludge was determined by an infrared spectroscopic oil analyzer. The degradation effects of strain Rheinheimera sp.JN2 on petroleum hydrocarbons in oily sludge under different pH conditions are as follows: ...

Embodiment 3

[0058] Example 3: Degradation effect of Rheinheimera sp.JN2 on petroleum hydrocarbons in oily sludge at different temperatures

[0059] Oily sludge from Liaohe Oilfield was taken, air-dried, ground, and passed through a 1mm sieve. Weigh 40 g of the above oily sludge into an Erlenmeyer flask, add OD600=1 activated bacterial liquid of Rheinheimera sp.JN2 according to 10% inoculation amount, adjust the water-to-soil ratio to 2:5 with distilled water, and seal it with a breathable sealing film. The Erlenmeyer flasks were placed in constant temperature incubators at 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, and 50°C for static culture, and samples were taken after 25 days. Spectrophotometer was used to determine the total petroleum hydrocarbon content in oily sludge. The degradation effect of strain Rheinheimera sp.JN2 on total petroleum hydrocarbons in oily sludge at different temperatures is as follows: Figure 5 shown. Depend on Figure 5 It can be seen that the strain...

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Abstract

The invention discloses high-efficiency degrading bacteria JN2 for petroleum hydrocarbons in oily sludge and its application. The degrading bacteria JN2 are preserved in the China general microbiological culture collection center, have a preservation number of CGMCC NO. 14973 and are classified as Rheinheimera sp. The bacteria JN5 with a petroleum hydrocarbon degradation function are used for treating oily sludge through petroleum hydrocarbon degradation. In 30 days, the bacteria JN2 have the oily sludge total petroleum hydrocarbon (TPH) degradation rate of 93.3%. The optimal degradation conditions of the bacteria to petroleum contaminants comprise a temperature of 15-50 DEG C and pH of 7-11. The bioremediation method for oily sludge has the advantages of high efficiency, low cost and environmental friendliness.

Description

technical field [0001] The invention relates to the technical field of bioremediation, in particular to a bacterial strain for degrading and repairing oily sludge and its application in degrading oily sludge. Background technique [0002] Since the 20th century, petroleum has become one of the most important energy sources for human beings. While being exploited and used in large quantities, the harm caused by petroleum and its processed products to the environment and human beings has become increasingly serious. Since China's reform and opening up, my country's annual output of crude oil has exceeded 100 million tons. China has become one of the world's top ten oil-producing countries and has more than 400 oil fields and oil and gas fields for exploration and development, which are distributed in 25 countries accounting for about 3% of the country's land area. Provinces (municipalities) and autonomous regions. However, in the process of rapid economic and technological dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/01C02F101/32C02F103/10
CPCC02F11/02C02F2101/32C02F2103/10C12N1/20C12N1/205C12R2001/01
Inventor 叶正芳姜楠赵泉林
Owner PEKING UNIV
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