17 results about "Accelerated solvent extraction" patented technology

The invention provides a full-automatic accelerated solvent extraction method for extracting antibiotics in soil, and belongs to the technical field of chemical detection. The extraction method provided by the invention comprises the following steps: mixing a soil sample with a mixed antibiotic internal standard solution, a purifying agent and methanol to obtain a to-be-extracted soil sample; the soil sample to be extracted is extracted in an accelerated solvent extraction evaporator ExtrevaASE, and a liquid to be detected is obtained; the extraction solvent is a mixed solution of a citric acid-trisodium citrate buffer solution and acetonitrile; the pH value of the citric acid-trisodium citrate buffer solution is 3 to 4. According to the method, an accelerated solvent extraction evaporator ExtrevaASE is adopted for extraction, so that the extraction process is fully automatic, manpower resources are saved, meanwhile, the extraction pressure is low (200 psi), and a mixed solution of a citric acid-trisodium citrate buffer solution and acetonitrile is adopted as an extraction solvent, so that the extraction effect is improved.

The present invention provides a method for determining the content of diazepam, chlorpromazine and ribavirin in animal-derived food by accelerated solvent extraction-liquid chromatography-mass spectrometry, which belongs to the technical field of residual chemical substance analysis and detection. The present invention uses an accelerated solvent extractor, optimizes the pretreatment extraction and purification methods, and then uses high-performance liquid chromatography-tandem mass spectrometry for analysis, so that the operation process is fast and simple, the detection accuracy is high, and it can provide good detection technology support, and save a lot of time and economic costs. Compared with traditional methods, the experimental time can be reduced from two days to half a day.

The invention relates to the technical field of compound detection, and discloses a method for determining the content of azodicarbonamide in a rubber and plastic thermal insulation product. The method comprises the following steps: 1) preparing a standard solution; 2) drawing a standard curve; 3) accelerated solvent extraction (ASE) treatment is performed on the sample, and specific parameters of the sample ASE treatment are as follows: the extraction solvent is N, N-dimethylformamide (DMF) with the volume of 100%, the extraction pressure is 1500 psi, the solvent flushing volume is 60% of the volume of a pool, the extraction pool is a stainless steel extraction pool with the volume of 34 mL, nitrogen purging is performed for 100 s, the extraction temperature is 65-85 DEG C, the extraction time is 5-15 min, and the cycle index is 1-2 times; (4) the sample is subjected to high performance liquid chromatography (HPLC) determination, and the test conditions of HPLC are as follows: a chromatographic column is ZORBAX SB-Aq (4.6 mm * 250 mm, 5 [mu] m); the column temperature is 30-40 DEG C; the mobile phase comprises the following components in percentage by volume: 80-100% of ultrapure water and 0-20% of methanol; the flow rate is 0.8 to 1.0 mL / min; and the wavelength of a DAD detector is 244 to 275 nm. According to the method, the ASE pretreatment technology is applied, selective extraction and online purification can be achieved through the ASE method, and the content of azodicarbonamide in the rubber and plastic heat preservation product can be accurately detected.

An Oncidium plant extract and methods for treating diabetes mellitus, liver protection and preventing acute respiratory distress syndrome thereof are provided, the steps for extracting the Oncidium plant extract include taking an Oncidium plant sample, drying and grinding the sample to obtain a ground Oncidium plant sample, and then extracting the ground Oncidium plant sample in a solvent by an accelerated solvent extraction or an ultrasonic extraction to obtain an Oncidium plant extract.

The invention provides a method for automatically extracting polybrominated organic pollutants in a bottom mud sample, which comprises the following steps: step 1, collecting the bottom mud sample, wrapping the bottom mud sample with an aluminum foil, and performing low-temperature cryopreservation; 2, freeze-drying the sample by using a freeze-drying machine, and then sieving by using a metal sieve to remove impurities; step 3, performing parameter and process setting on an automatic sample pretreatment system built in a laboratory; 4, mixing the pretreated bottom mud with diatomite, filling the mixture into a stainless steel extraction pool, placing a filter membrane at the bottom of the pool body, placing the pool body into a sample extraction position of an accelerated solvent extraction instrument, and extracting a sample to obtain a bottom mud extracting solution; step 5, each time one sample is extracted, the obtained extracting solution is automatically transferred to a full-automatic gel purification quantitative concentration system instrument by a mechanical arm to be concentrated and dissolved, and then is transferred and submitted to a gel permeation chromatography by a sample injection needle of the instrument; step 6, concentrating the extracting solution, and eluting and purifying by using gel permeation chromatography; and 7, introducing the concentrated solution purified by the gel permeation chromatography into a concentrator through an internal pipeline, concentrating in two modes of pressure reduction and heating, and finally transferring the concentrated solution into a brown small sample injection bottle through a sample injection needle to obtain an extracting solution of the sample. The invention aims to provide a method for efficiently and quickly extracting brominated organic pollutants in bottom mud, manual operation is less, the sample pretreatment time is shortened, the extraction efficiency is improved, and convenience is provided for subsequent compound analysis and identification.

The invention discloses a method for efficiently extracting high-activity tremella polysaccharide (TFPS) through an accelerated solvent extraction (ASE) method, and belongs to the technical field of natural product extraction. Aiming at the defects of long time consumption, low efficiency, easy degradation of active components and the like of a traditional hot water extraction (HWE) method, the method adopts a high-temperature and high-pressure static extraction technology, a dried and crushed tremella sample (particle size is less than or equal to 0.5 mm) and diatomite are mixed and then are filled into an extraction tank, and static extraction is performed for multiple times at high temperature and high pressure to obtain a white fungus extract; the methanol-chloroform mixed solvent is used for multiple times of automatic cyclic extraction, and the treatment time of a single batch of samples is short. The obtained ASE-TFPS is rich in molecular weight lt; and in a H2O2-induced caenorhabditis elegans oxidative stress model, the ROS clearance rate is high, the SOD enzyme activity is improved, and the recovery level is equivalent to that of a normal control group. According to the method, rapid, efficient and high-activity extraction of the tremella polysaccharide is achieved, and the product has remarkable application value in the field of functional food, anti-aging cosmetics and antioxidant drug development.

The application provides a preparation method of sporopollenin purification material and application thereof. The sporopollenin purification material is prepared by adding plant spores and pollen into an extraction tank and using an accelerated solvent extraction instrument, using methanol as an extraction solvent, an extraction temperature of 70 DEG C, an extraction pressure of 1500 psi, an extraction time of 5 min, and 2 cycles, washing the plant spores and pollen with a solvent acetonitrile / water / formic acid (70:28:2, v / v / v) after extraction, and drying at 60 DEG C for 12 hours, so that a hydrophilic solid-phase extraction adsorbent is successfully prepared. The sporopollenin purification material is rich in source, good in biocompatibility, non-toxic and non-polluted. The prepared sporopollenin purification material is complete in appearance, stable in property, stronger in binding force, cleaner in spore cavity, and has a hydrophilic adsorption effect due to the functional groups of multiple hydroxyl groups on the surface, so that the polar compounds can be well adsorbed.

The application discloses a method for determining the content of disperse red 9 in PET colored products by using high performance liquid chromatography. The application uses accelerated solvent extraction as a pretreatment technology, adds neutral alumina as a dispersion layer and an adsorbent (mixed with the sample) in batches, strengthens solvent penetration, uses a methanol-acetone binary mixed solvent as an extraction solvent system, and realizes efficient and controllable extraction of the target object under specific conditions. The ASE extraction liquid is purified by using an HLB solid phase extraction column, the interference in the matrix is effectively removed, the specificity of the method is improved, and the signal-to-noise ratio of the chromatographic peak is improved. The method has high pretreatment efficiency, good repeatability and low solvent consumption, can realize trace detection at the level of 0.1 mg / kg, can fully meet the compliance detection requirements of disperse red 9 in PET colored products for food contact, and is suitable for popularization and use in various food safety detection institutions and enterprise quality control laboratories.

The application provides a kind of automated extraction of polybrominated organic pollutants in sediment sample method, comprising the following steps: step 1: collecting sediment sample, wrapping with aluminum foil, low temperature frozen preservation;Step 2: freeze-drying machine is used to freeze-dry sample, then impurity is removed by screening with metal screen;Step 3: the parameter and process setting of the automatic sample pretreatment system built in laboratory are carried out;Step 4: the pretreated sediment is mixed with diatomite, then loaded into stainless steel extraction tank, filter membrane is placed at the bottom of tank body, placed in sample extraction position of accelerated solvent extraction instrument, the sample is extracted, and sediment extraction liquid is obtained;Step 5: every extraction is completed, the obtained extraction liquid is automatically transferred to full-automatic gel purification quantitative concentration system instrument by mechanical arm, and concentrated and dissolved again, then submitted to gel permeation chromatography by instrument sampling needle;Step 6: after the extraction liquid is concentrated, gel permeation chromatography elution purification is used;Step 7: the concentrated liquid after gel permeation chromatography purification is introduced into concentrator through internal pipeline, concentrated by two modes of reduced pressure and heating, finally transferred to brown sample vial by sampling needle, and the extraction liquid of sample is obtained.The purpose of the application is to provide a kind of efficient and rapid extraction method of brominated organic pollutants in sediment, which has less manual operation, shortens the time of sample pretreatment, improves the extraction efficiency, and provides convenience for subsequent compound analysis and identification.

An accelerated solvent extraction apparatus comprising: a mixing chamber for combining a first gas stream or a mixture gas stream and a first solvent stream or a solvent mixture stream and providing the gas and solvent mixture to a plurality of extraction vessels containing a first plurality of samples; a temperature control zone for maintaining the plurality of extraction vessels at a predetermined temperature; a first rack for fluidly coupling the plurality of extraction vessels to a first plurality of evaporation flasks, each evaporation flask fluidly coupled to a respective extraction vessel; and a second rack for fluidly coupling a second plurality of evaporation flasks to a second gas stream to evaporate and concentrate a second plurality of extracted samples, wherein the mixing chamber and the first rack are operated simultaneously with the second rack.

The invention relates to the technical field of navigation instruments, in particular to an ASE (Accelerated Solvent Extraction) light source capable of stabilizing average wavelength and an optical fiber gyroscope, which adopt a two-way backward light path structure with high power, wide spectral width and stable average wavelength, and comprise a two-stage erbium-doped optical fiber structure in the light path. The temperature sensitivity of the average wavelength of output light of the ASE light source is reduced by adjusting the lengths of the two sections of erbium-doped optical fibers, a long-period fiber grating is designed in a light path, the average wavelength of a reflectivity peak of the long-period fiber grating and the average wavelength of the output light of the ASE light source are increased along with temperature rise, average wavelength drift caused by temperature is reduced, a Lyot depolarizer is designed in the light path, and the average wavelength drift of the output light of the ASE light source is reduced. The ASE light source is arranged behind the pump laser, the influence of polarization of output light of the pump laser on the average wavelength of the output light of the ASE light source is reduced, in addition, a Faraday rotating lens is designed, the reflected light is orthogonal to the polarization state of incident light, and the influence of polarization of the output light of the pump laser on the average wavelength of the output light of the ASE light source is further reduced.

To provide a natural compound and its use for the manufacture of a drug for treating diabetes mellitus, protecting the liver, and preventing acute respiratory distress syndrome (ARDS).SOLUTION: An Oncidium plant extract is provided. The preparation steps therefor include the steps of: taking an Oncidium plant sample; drying and grinding the sample to obtain a ground Oncidium plant sample; and extracting the ground Oncidium plant sample in a solvent through accelerated solvent extraction or ultrasonic extraction to obtain an Oncidium plant extract.SELECTED DRAWING: Figure 1

The application belongs to the technical field of detection, and particularly relates to a detection method and application of a sensitizing disperse dye in soil and sediment. The detection method of the sensitizing disperse dye comprises the following steps: (1) obtaining and extracting a sample, then treating the extracted sample to obtain a to-be-tested sample solution; configuring a standard solution, testing the standard solution by liquid chromatography, and establishing a standard curve of concentration and peak area; (2) detecting the to-be-tested sample solution by liquid chromatography, and quantifying by an external standard method to realize detection and analysis of the sensitizing disperse dye in the sample. The liquid chromatography detection method of the sensitizing disperse dye in soil and sediment provided by the application mainly aims at the technical problems of long time consumption, large solvent consumption, and decomposition of heat-sensitive dyes under high-temperature conditions in the current technology, and has the advantages of short processing period, relatively low instrument and technology cost, and small solvent consumption compared with the Soxhlet extraction method, accelerated solvent extraction, and oscillation extraction.

An accelerated solvent extraction apparatus includes a mixing chamber for combining a first flow of a gas or a mixture gases and first flow of a solvent or a mixture of solvents and providing the gas and solvent mixture to a plurality of extraction vessels containing a first plurality of samples; a temperature controlled zone for maintaining the plurality of extraction vessels at a predetermined temperature; a first gantry for fluidly coupling the plurality of extraction vessels to a first plurality of evaporation bottles, each evaporation bottle fluidly coupled to a respective extraction vessel; and a second gantry for fluidly coupling a second plurality of evaporation bottles to a second flow of gas to evaporate and concentrate a second plurality of extracted samples, wherein the mixing chamber and first gantry operates concurrently with the second gantry.

The invention discloses a method for detecting neonicotinoid compounds in sediments, which comprises the following steps: S100, drying, grinding and sieving a sediment sample, uniformly mixing the sediment sample with diatomite, extracting by using a methanol-acetonitrile mixed solvent as an extracting agent and adopting an accelerated solvent extraction method, and concentrating an extracting solution to be purified; s200, purifying the concentrated extract by using a solid-phase extraction column, collecting the eluent, concentrating, and filtering to obtain a sample to be detected; and S300, analyzing the concentration of the neonicotinoid compounds in the sample to be detected by using high performance liquid chromatography-tandem triple quadrupole mass spectrometry. The method is simple to operate, rapid and time-saving, has relatively good sensitivity and precision, and can be applied to detection of river sediments in a chemical aggregation area.

The invention relates to the technical field of analysis and detection, in particular to a method for detecting drugs and personal care products in soil. According to the extraction, separation and enrichment steps of the traditional Chinese medicines and the personal care products, accelerated solvent extraction and solid-phase extraction are combined, so that the extraction rate of the various medicines and the personal care products in the soil can be remarkably increased, the sample loss rate is reduced, and the extraction time is shortened. According to the method, accelerated solvent extraction and solid-phase extraction are combined with a high-performance liquid chromatography coupling technique, isocratic elution is performed for 15-20 minutes, so that various trace drugs and personal care products with different structures and different polarities, such as ibuprofen, carbamazepine, bisphenol A and triclosan, which are remained in soil can be simply, quickly, accurately and universally detected, and the sensitivity is high. Moreover, the detection method provided by the invention is high in accuracy and good in reproducibility, and a rapid, accurate, visual, qualitative and quantitative determination method for the soil traditional Chinese medicines and the personal care products is provided for environmental detection departments, governments and enterprises.

The invention provides an oncidium extract and application of the oncidium extract in preparation of drugs for treating diabetes, protecting the liver and preventing acute respiratory distress syndrome, and the oncidium extract is prepared by the following steps: firstly, taking an oncidium sample, drying and grinding the oncidium sample to obtain a ground oncidium sample; and placing the ground oncidium sample in a solvent, and extracting by an accelerated solvent extraction method or an ultrasonic oscillation extraction method to obtain the oncidium extract.