9 results about "Accelerated solvent extraction" patented technology

The invention provides a full-automatic accelerated solvent extraction method for extracting antibiotics in soil, and belongs to the technical field of chemical detection. The extraction method provided by the invention comprises the following steps: mixing a soil sample with a mixed antibiotic internal standard solution, a purifying agent and methanol to obtain a to-be-extracted soil sample; the soil sample to be extracted is extracted in an accelerated solvent extraction evaporator ExtrevaASE, and a liquid to be detected is obtained; the extraction solvent is a mixed solution of a citric acid-trisodium citrate buffer solution and acetonitrile; the pH value of the citric acid-trisodium citrate buffer solution is 3 to 4. According to the method, an accelerated solvent extraction evaporator ExtrevaASE is adopted for extraction, so that the extraction process is fully automatic, manpower resources are saved, meanwhile, the extraction pressure is low (200 psi), and a mixed solution of a citric acid-trisodium citrate buffer solution and acetonitrile is adopted as an extraction solvent, so that the extraction effect is improved.

The invention discloses a method for efficiently extracting high-activity tremella polysaccharide (TFPS) through an accelerated solvent extraction (ASE) method, and belongs to the technical field of natural product extraction. Aiming at the defects of long time consumption, low efficiency, easy degradation of active components and the like of a traditional hot water extraction (HWE) method, the method adopts a high-temperature and high-pressure static extraction technology, a dried and crushed tremella sample (particle size is less than or equal to 0.5 mm) and diatomite are mixed and then are filled into an extraction tank, and static extraction is performed for multiple times at high temperature and high pressure to obtain a white fungus extract; the methanol-chloroform mixed solvent is used for multiple times of automatic cyclic extraction, and the treatment time of a single batch of samples is short. The obtained ASE-TFPS is rich in molecular weight lt; and in a H2O2-induced caenorhabditis elegans oxidative stress model, the ROS clearance rate is high, the SOD enzyme activity is improved, and the recovery level is equivalent to that of a normal control group. According to the method, rapid, efficient and high-activity extraction of the tremella polysaccharide is achieved, and the product has remarkable application value in the field of functional food, anti-aging cosmetics and antioxidant drug development.

The application provides a preparation method of sporopollenin purification material and application thereof. The sporopollenin purification material is prepared by adding plant spores and pollen into an extraction tank and using an accelerated solvent extraction instrument, using methanol as an extraction solvent, an extraction temperature of 70 DEG C, an extraction pressure of 1500 psi, an extraction time of 5 min, and 2 cycles, washing the plant spores and pollen with a solvent acetonitrile / water / formic acid (70:28:2, v / v / v) after extraction, and drying at 60 DEG C for 12 hours, so that a hydrophilic solid-phase extraction adsorbent is successfully prepared. The sporopollenin purification material is rich in source, good in biocompatibility, non-toxic and non-polluted. The prepared sporopollenin purification material is complete in appearance, stable in property, stronger in binding force, cleaner in spore cavity, and has a hydrophilic adsorption effect due to the functional groups of multiple hydroxyl groups on the surface, so that the polar compounds can be well adsorbed.

The application discloses a method for determining the content of disperse red 9 in PET colored products by using high performance liquid chromatography. The application uses accelerated solvent extraction as a pretreatment technology, adds neutral alumina as a dispersion layer and an adsorbent (mixed with the sample) in batches, strengthens solvent penetration, uses a methanol-acetone binary mixed solvent as an extraction solvent system, and realizes efficient and controllable extraction of the target object under specific conditions. The ASE extraction liquid is purified by using an HLB solid phase extraction column, the interference in the matrix is effectively removed, the specificity of the method is improved, and the signal-to-noise ratio of the chromatographic peak is improved. The method has high pretreatment efficiency, good repeatability and low solvent consumption, can realize trace detection at the level of 0.1 mg / kg, can fully meet the compliance detection requirements of disperse red 9 in PET colored products for food contact, and is suitable for popularization and use in various food safety detection institutions and enterprise quality control laboratories.

The application provides a kind of automated extraction of polybrominated organic pollutants in sediment sample method, comprising the following steps: step 1: collecting sediment sample, wrapping with aluminum foil, low temperature frozen preservation;Step 2: freeze-drying machine is used to freeze-dry sample, then impurity is removed by screening with metal screen;Step 3: the parameter and process setting of the automatic sample pretreatment system built in laboratory are carried out;Step 4: the pretreated sediment is mixed with diatomite, then loaded into stainless steel extraction tank, filter membrane is placed at the bottom of tank body, placed in sample extraction position of accelerated solvent extraction instrument, the sample is extracted, and sediment extraction liquid is obtained;Step 5: every extraction is completed, the obtained extraction liquid is automatically transferred to full-automatic gel purification quantitative concentration system instrument by mechanical arm, and concentrated and dissolved again, then submitted to gel permeation chromatography by instrument sampling needle;Step 6: after the extraction liquid is concentrated, gel permeation chromatography elution purification is used;Step 7: the concentrated liquid after gel permeation chromatography purification is introduced into concentrator through internal pipeline, concentrated by two modes of reduced pressure and heating, finally transferred to brown sample vial by sampling needle, and the extraction liquid of sample is obtained.The purpose of the application is to provide a kind of efficient and rapid extraction method of brominated organic pollutants in sediment, which has less manual operation, shortens the time of sample pretreatment, improves the extraction efficiency, and provides convenience for subsequent compound analysis and identification.

An accelerated solvent extraction apparatus comprising: a mixing chamber for combining a first gas stream or a mixture gas stream and a first solvent stream or a solvent mixture stream and providing the gas and solvent mixture to a plurality of extraction vessels containing a first plurality of samples; a temperature control zone for maintaining the plurality of extraction vessels at a predetermined temperature; a first rack for fluidly coupling the plurality of extraction vessels to a first plurality of evaporation flasks, each evaporation flask fluidly coupled to a respective extraction vessel; and a second rack for fluidly coupling a second plurality of evaporation flasks to a second gas stream to evaporate and concentrate a second plurality of extracted samples, wherein the mixing chamber and the first rack are operated simultaneously with the second rack.

The application belongs to the technical field of detection, and particularly relates to a detection method and application of a sensitizing disperse dye in soil and sediment. The detection method of the sensitizing disperse dye comprises the following steps: (1) obtaining and extracting a sample, then treating the extracted sample to obtain a to-be-tested sample solution; configuring a standard solution, testing the standard solution by liquid chromatography, and establishing a standard curve of concentration and peak area; (2) detecting the to-be-tested sample solution by liquid chromatography, and quantifying by an external standard method to realize detection and analysis of the sensitizing disperse dye in the sample. The liquid chromatography detection method of the sensitizing disperse dye in soil and sediment provided by the application mainly aims at the technical problems of long time consumption, large solvent consumption, and decomposition of heat-sensitive dyes under high-temperature conditions in the current technology, and has the advantages of short processing period, relatively low instrument and technology cost, and small solvent consumption compared with the Soxhlet extraction method, accelerated solvent extraction, and oscillation extraction.

The invention discloses a method for detecting neonicotinoid compounds in sediments, which comprises the following steps: S100, drying, grinding and sieving a sediment sample, uniformly mixing the sediment sample with diatomite, extracting by using a methanol-acetonitrile mixed solvent as an extracting agent and adopting an accelerated solvent extraction method, and concentrating an extracting solution to be purified; s200, purifying the concentrated extract by using a solid-phase extraction column, collecting the eluent, concentrating, and filtering to obtain a sample to be detected; and S300, analyzing the concentration of the neonicotinoid compounds in the sample to be detected by using high performance liquid chromatography-tandem triple quadrupole mass spectrometry. The method is simple to operate, rapid and time-saving, has relatively good sensitivity and precision, and can be applied to detection of river sediments in a chemical aggregation area.

The invention relates to the technical field of analysis and detection, in particular to a method for detecting drugs and personal care products in soil. According to the extraction, separation and enrichment steps of the traditional Chinese medicines and the personal care products, accelerated solvent extraction and solid-phase extraction are combined, so that the extraction rate of the various medicines and the personal care products in the soil can be remarkably increased, the sample loss rate is reduced, and the extraction time is shortened. According to the method, accelerated solvent extraction and solid-phase extraction are combined with a high-performance liquid chromatography coupling technique, isocratic elution is performed for 15-20 minutes, so that various trace drugs and personal care products with different structures and different polarities, such as ibuprofen, carbamazepine, bisphenol A and triclosan, which are remained in soil can be simply, quickly, accurately and universally detected, and the sensitivity is high. Moreover, the detection method provided by the invention is high in accuracy and good in reproducibility, and a rapid, accurate, visual, qualitative and quantitative determination method for the soil traditional Chinese medicines and the personal care products is provided for environmental detection departments, governments and enterprises.