Method for automatically extracting polybrominated organic pollutants in bottom mud sample

Abstract

The invention provides a method for automatically extracting polybrominated organic pollutants in a bottom mud sample, which comprises the following steps: step 1, collecting the bottom mud sample, wrapping the bottom mud sample with an aluminum foil, and performing low-temperature cryopreservation; 2, freeze-drying the sample by using a freeze-drying machine, and then sieving by using a metal sieve to remove impurities; step 3, performing parameter and process setting on an automatic sample pretreatment system built in a laboratory; 4, mixing the pretreated bottom mud with diatomite, filling the mixture into a stainless steel extraction pool, placing a filter membrane at the bottom of the pool body, placing the pool body into a sample extraction position of an accelerated solvent extraction instrument, and extracting a sample to obtain a bottom mud extracting solution; step 5, each time one sample is extracted, the obtained extracting solution is automatically transferred to a full-automatic gel purification quantitative concentration system instrument by a mechanical arm to be concentrated and dissolved, and then is transferred and submitted to a gel permeation chromatography by a sample injection needle of the instrument; step 6, concentrating the extracting solution, and eluting and purifying by using gel permeation chromatography; and 7, introducing the concentrated solution purified by the gel permeation chromatography into a concentrator through an internal pipeline, concentrating in two modes of pressure reduction and heating, and finally transferring the concentrated solution into a brown small sample injection bottle through a sample injection needle to obtain an extracting solution of the sample. The invention aims to provide a method for efficiently and quickly extracting brominated organic pollutants in bottom mud, manual operation is less, the sample pretreatment time is shortened, the extraction efficiency is improved, and convenience is provided for subsequent compound analysis and identification.