A method for the isolation and extraction of nucleic acid binding proteins in solid tissue cells
By preparing single-cell suspensions through mechanical grinding and enzymatic digestion, combined with formaldehyde cross-linking and Ti4+-IMAC capture, the problems of low extraction efficiency of nucleic acid-binding proteins and interference from plasma proteins in solid tissues were solved, achieving efficient and specific extraction of nucleic acid-binding proteins, which is suitable for a variety of downstream analyses.
CN116162125BActive Publication Date: 2026-06-23SHANGHAI JIAOTONG UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHANGHAI JIAOTONG UNIV
- Filing Date
- 2023-03-02
- Publication Date
- 2026-06-23
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Figure CN116162125B_ABST
Abstract
The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.
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