The invention relates to a cascade acoustic microstructure optical fiber long cycle grating interferometer which comprises a light source, a 3dB coupler, a microstructure optical fiber, two three-dimensional regulation frames, a signal generator, a piezoelectric ceramic chip, an acoustic amplifier, an acoustic damper, a spectrum analyzer, a connecting single-mode optical fiber and a lead, and belongs to the technical field of optical fiber induction. A sine electric signal is generated through the signal generator, acts on the piezoelectric ceramic chip, is converted into an acoustic signal, amplified through the acoustic amplifier and laterally loaded to the microstructure optical fiber to form an acoustic long cycle optical fiber grating, and then a Mach-Zehnder interference effect is formed through a high reverse film; and an induction optical fiber is corroded to generate an evanescent field and is arranged in an environment to be measured. In addition, a functional material is filled into the cladding air hole of the microstructure optical fiber, so that an electric field, a magnetic filed and the like can be measured and the induction sensitivity is further improved. The cascade acoustic microstructure optical fiber long cycle grating interferometer has the advantages that the structure is simple, the tunability is good, the sensitivity is high, and refractive index, temperature, the electric field, the magnetic field, gas concentration and other parameters can be induced.