The invention relates to a comb spectrum generation system based on a cyclic frequency shift pattern and an application method of the comb spectrum generation system based on a cyclic frequency shift pattern. The comb spectrum generation system based on the cyclic frequency shift pattern comprises an external-cavity semiconductor laser, a 3 dB coupler, a polarization controller, an intelligence quotient (IQ) modulator, a phase modulator, an optical frequency spectrum analyzer, a cosine microwave signal source, a phase shifter, an erbium-doped optical fiber amplifier, an optical filter, an adjustable optical delay line, a direct current bias supply and a section of optical fiber. Cosine radio-frequency signals, reversed cosine radio-frequency signals, sine radio frequency signals with same frequency, and reversed sine radio frequency signals with same frequency are inputted into the IQ modulator. Down-converted signals of optical carrier can be produced through adding appropriate bias voltage to a direct current electrode. The down-converted frequency is four radio frequency (RF) electrode incoming frequency. Elements of coupled seed light and the down-converted light of the optical carrier are modulated by the IQ modulator in a loop circuit. Flat comb spectrum can be obtained through reasonable gain conditions of the erbium-doped optical fiber amplifier and choices of the optical carrier bandwidth.