This invention provides a quantitative detection method for exosomes based on
aptamer-functionalized tilted
fiber optic gratings combined with gold
nanoparticle signal amplification. The detection device consists of a
broadband light source, a single-mode
fiber, an electrically driven
polarization controller,
fiber optic clamp 1, a TFBG
aptamer sensor, a
reaction chamber, an optical three-dimensional adjustment frame, another fiber optic clamp 2, and a
spectrometer. The detection principle can be summarized as follows: light emitted from the
broadband light source is transmitted through the single-mode fiber to the electrically driven
polarization controller, where the incident light is modulated into a P-polarization state. The P-state light is transmitted to the TFBG
aptamer sensor, which is tightly suspended and fixed in the
reaction chamber by the fiber optic clamp. The final output
light signal is presented as a transmission spectrum on the
spectrometer. During the detection process, the MUC1 aptamer layer on the surface of the TFBG aptamer sensor selectively captures exosomes produced by
breast cancer cell lines. Furthermore, by combining with CD63 aptamers to aggregate gold nanoparticles, the
refractive index change on the surface of the TFBG aptamer sensor is amplified, further amplifying the drift in the transmission spectrum. By comparing the relationship between different
exosome solution concentrations and the amount of transmission spectrum drift, rapid and
highly sensitive quantitative detection of exosomes is achieved. This invention provides a new method for the quantitative detection of exosomes that is
highly sensitive, simple to operate, and rapid, and has broad application value in early diagnosis and prognosis in clinical
medicine.