The invention relates to an optical frequency comb, and specifically relates to a deterministic optical soliton frequency comb generation system and method based on a micro-cavity. The system comprises a pump laser system, an auxiliary laser system, a packaged micro-cavity resonant cavity, and a temperature controller. The pump laser system and the auxiliary laser system consist of a narrow line width laser with the fixed wavelength or adjustable wavelength, a light amplifier, a polarization controller and an optical circulator. The temperature control system consists of a semiconductor coolerand a controller of the semiconductor cooler, or consists of an on-chip integrated metal lien and a current control system. The system employs the temperature tuning for the position of the harmonicpeak of the micro-cavity resonant cavity, achieves the deterministic generation of a micro-cavity optical soliton frequency comb. According to the scheme provided by the invention, the system is simple in structure, is low in cost, and is high in operability, solves a problem of the dependence on a high-frequency device in a generation process of the micro-cavity optical soliton frequency comb, and has a high practical value in the application field of the micro-cavity optical soliton frequency comb in future.