The invention discloses a preparation method of stability-enhanced
alginic acid-
keratin-based double-coated nanoparticles. The preparation method comprises the following steps: carrying out controllable enzymolysis,
enzyme deactivation,
centrifugation and freeze-
drying on
keratin to obtain
keratin nanoparticles formed by self-
assembly; dispersing the keratin nanoparticles in an acidic
buffer solution to carry out structure development, adding
bioactive molecules, and adjusting the pH to be neutral to recombine and close the keratin
nanoparticle structure to obtain a keratin
nanoparticle solution loaded with the
bioactive molecules; the preparation method comprises the following steps: taking keratin as a template, dispersing the keratin into a
sodium alginate solution, dropwise adding into a
calcium chloride solution, cross-linking to form a
calcium alginate shell, dialyzing or centrifuging to remove free
bioactive molecules, and freeze-
drying to obtain the bioactive molecule loaded
alginic acid-keratin-based double-coated nanoparticles. The invention also discloses double-coated nanoparticles and application thereof, through controllable enzymolysis and
pH regulation and control, the self-
assembly and bioactive molecule encapsulation of the keratin nanoparticles are improved, and the double-coated nanoparticles with regular shape, good dispersibility, good redissolvability and good
environmental stability are formed.