Multi-shell-structure quantum dot composite particle, and high-fluorescent-brightness quantum dot probe and preparation method thereof

A technology of composite particles and quantum dots, which is applied in the direction of chemical instruments and methods, luminescent materials, material inspection products, etc., can solve the problem of affecting the accuracy and repeatability of the detection response of the substance to be tested, severe impact, unstable physical and chemical properties, etc. problems, achieve good biological adaptability and stability, good effect, and improve the effect of fluorescence brightness

Inactive Publication Date: 2013-02-13
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large specific surface area of ​​quantum dots, their own physical and chemical properties are unstable, and they are severely affected by the environment in applications, which greatly affects the accuracy and repeatability of the detection response of the substance to be tested.

Method used

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  • Multi-shell-structure quantum dot composite particle, and high-fluorescent-brightness quantum dot probe and preparation method thereof
  • Multi-shell-structure quantum dot composite particle, and high-fluorescent-brightness quantum dot probe and preparation method thereof
  • Multi-shell-structure quantum dot composite particle, and high-fluorescent-brightness quantum dot probe and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0052] 1.1 The CdTe quantum dots were synthesized by the aqueous phase method. First synthesize the NaHTe required for the preparation of CdTe quantum dots: combine Te powder with NaBH 4 Mix according to the molar ratio of 1:10, add 2ml of water, heat in a water bath at 60 degrees Celsius under nitrogen protection and stir until the solution turns lavender. 0.4 mmol of CdCl 2 2.5H 2 O was dissolved in 25 mL of water, 0.6 mmol of thioglycolic acid (TGA) was added, stirred, and then 1M NaOH solution was added until the pH value of the solution was adjusted to 11.2. The solution was deoxygenated with nitrogen for 30 minutes, then injected into the NaHTe solution prepared above, and heated to reflux at 100 degrees Celsius for 10 minutes to obtain water-soluble green-emitting CdTe quantum dots (particle size: 1.5 nm, wavelength: 520 nm).

[0053] 1.2 Add 0.5 mmol of CdCl 2 2.5H 2 Dissolve O in 10 mL of water, add 0.75 mmol TGA, that is, the molar ratio of mercapto compound to ...

Embodiment 2

[0061] 2.1 CdTe quantum dots were synthesized by the aqueous phase method. First synthesize the NaHTe required for the preparation of CdTe quantum dots: combine Te powder with NaBH 4 Mix according to the molar ratio of 1:10, add 2ml of water, heat in a water bath at 60 degrees Celsius under nitrogen protection and stir until the solution turns lavender. 0.4 mmol of CdCl 2 2.5H 2 O was dissolved in 25 mL of water, 0.6 mmol of mercaptoethanol was added, stirred, and then 1M NaOH solution was added until the pH value of the solution was adjusted to 11.2. The solution was deoxygenated with nitrogen for 30 minutes, then injected into the NaHTe solution prepared above, and heated to reflux at 100 degrees Celsius for 4 hours to obtain water-soluble yellow fluorescent CdTe quantum dots (particle size: 3.2 nm, wavelength: 580 nm) .

[0062] 2.2 Add 0.5 mmol of CdCl 2 2.5H 2 Dissolve O in 10 mL of water, add 1 mmol TGA, that is, the molar ratio of mercapto compound to cation is 2:...

Embodiment 3

[0070] 3.1 The CdTe quantum dots were synthesized by the aqueous phase method. First synthesize the NaHTe required for the preparation of CdTe quantum dots: combine Te powder with NaBH 4 Mix according to the molar ratio of 1:10, add 2ml of water, heat in a water bath at 60 degrees Celsius under nitrogen protection and stir until the solution turns lavender. 0.4 mmol of CdCl 2 2.5H 2 O was dissolved in 25 mL of water, 0.6 mmol of thioglycolic acid (TGA) was added, stirred, and then 1M NaOH solution was added until the pH value of the solution was adjusted to 11.2. The solution was deoxygenated with nitrogen for 30 minutes, then injected into the NaHTe solution prepared above, and heated to reflux at 100 degrees Celsius for 18 hours to obtain water-soluble red fluorescent CdTe quantum dots (particle size: 3.48 nm, wavelength: 620 nm) ,like image 3 shown in (a). fluorescence spectrum at Figure 4 middle.

[0071] 3.2 Add 0.5 mmol of CdCl 2 2.5H 2 Dissolve O in 10 mL of ...

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Abstract

The invention discloses a multi-shell-structure quantum dot composite particle, and a high-fluorescent-brightness quantum dot probe and a preparation method thereof. The composite particle uses a quantum dot as a core, a semiconductor shell is coated outside the quantum dot, and a composite silicon dioxide shell is coated outside the semiconductor shell. A biological molecule can be connected to the composite silicon dioxide shell to form the probe. The composite particle and probe with required fluorescent wavelength can be obtained by controlling the reaction conditions. The method has strong operability. The obtained composite particle has favorable chemical and physical stability, biocompatibility and environmental stability. The formed probe has high stability and high fluorescent brightness, can have high application value in the fields of medicine and biology, and can be used as a common fluorescent probe, immunologic detection agent or any other biosensor.

Description

Technical field [0001] The present invention involves a quantum dot composite particle, its probe, and preparation method with a multi -shell structure containing high fluorescent brightness, especially a SIO with a water -soluble semiconductor quantum dot, semiconductor shell, and semiconductor cluster. 2 The quantum dot composite particles composed of the shell, the probe formed by the composite particles as the main ingredient and its preparation method. Background technique [0002] After decades of research and development, the research on traditional II-Vi and III-V semiconductor quantum dots has formed a series of complete preparation methods and theoretical systems.Related research on semiconductor quantum dots has begun to expand in the direction of application.Since the United States "Science" magazine reported in the field of fluorescence probes in 1998, scientists in various countries have begun to use the light emitting quantum dots for biological detection systems, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/70G01N33/52
Inventor 杨萍刘宁
Owner UNIV OF JINAN
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