52results about How to "High fluorescence brightness" patented technology

The invention discloses a multi-shell-structure quantum dot composite particle, and a high-fluorescent-brightness quantum dot probe and a preparation method thereof. The composite particle uses a quantum dot as a core, a semiconductor shell is coated outside the quantum dot, and a composite silicon dioxide shell is coated outside the semiconductor shell. A biological molecule can be connected to the composite silicon dioxide shell to form the probe. The composite particle and probe with required fluorescent wavelength can be obtained by controlling the reaction conditions. The method has strong operability. The obtained composite particle has favorable chemical and physical stability, biocompatibility and environmental stability. The formed probe has high stability and high fluorescent brightness, can have high application value in the fields of medicine and biology, and can be used as a common fluorescent probe, immunologic detection agent or any other biosensor.

The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.

This invention relates to a method for dyeing polymer microspheres to obtain fluorescent polymer microspheres. The method comprises: uniformly mixing fluorescein 0.01-80%, polymer microspheres 0.1-80%, emulsifier 0-10%, tackifier 0-10%, and solvent (one or more of good solvents and poor solvents) 18.9-99.89%, dyeing under 1-101 kPa in dark for 1-720 h, taking out the dyed polymer microspheres, and washing repeatedly. The obtained fluorescent polymer microspheres have such advantages as simple process, high fluorescent brightness, wide fluorescent spectrum range, uniform particle size distribution and low relative standard deviation, and can be used as absolute counting microspheres of flow cytometry, fluoroimmunoassay microspheres, biosensor, microfluidic chip, and calibrator of fluorescence microscope.

The invention relates to a microfluidic-control preparation method for a microsphere of a polymethylmethacrylate-coated cadmium telluride (CdTe) quantum dot, and relates to a preparation method for the microsphere of a polymethylmethacrylate-coated CdTe quantum dot. The microfluidic-control preparation method for the microsphere of the polymethylmethacrylate-coated (CdTe) quantum dot aims to solve the problems that the surface of the quantum dot needs to be modified and the quantum dot is easy to dissolve by the microsphere of the polymethylmethacrylate-coated CdTe quantum dot by adopting a traditional preparation method for the microsphere of the polymethylmethacrylate-coated CdTe quantum dot. The method comprises the following steps: preparing a sodium hydrogen telluride solution; preparing a CdTe quantum dot; preparing a polymer solution; connecting a microfluidic-control system; respectively putting the polymer solution and silicone oil into injectors A and B of the microfluidic-control system; setting the propulsion speed of the injectors A and B; after starting, formed liquid beads flow into a rotary evaporator; and curing and evaporating the silicone oil to obtain the microsphere of the polymethylmethacrylate-coated CdTe quantum dot. In the microfluidic-control preparation method for the polymethylmethacrylate-coated CdTe quantum dot, the polymethylmethacrylate-coated CdTe quantum dot is selected, therefore, fluorescence is stable, and the microsphere can be adjusted in size and can be used for field of fluorescent display and the like.

The invention discloses water-dispersible multicolour fluorescent polymer nanoparticles and a preparation method thereof. The water-dispersible multicolour fluorescent polymer nanoparticles are prepared by taking methyl methacrylate as a monomer, taking 4-ethyoxyl-9-allyl-1,8-naphthalimide (EANI), 4-amido-7-nitro-N-allylbenzo[1,2,5] oxadiazole (NBDAA), and Nile red methacrylate (NRME) as polymerizable fluorescent dyes, taking lauryl sodium sulphate (SDS) as a surfactant, and using a one-step miniemulsion polymerization method. The water-dispersible multicolour fluorescent polymer nanoparticles are great in dispersibility in water, quite high in fluorescent brightness, and capable of emitting multicolour fluorescence from blue to red under the excitation of a single wave. The water-dispersible multicolour fluorescent polymer nanoparticles obtained by the preparation method disclosed by the invention are uniform in size, and good in structure and light stability, has a fluorescence emission signal capable of being adjusted for many times in a wide interval under a single excitation wavelength, is simple in synthesis route, convenient to use, suitable for amplification synthesis and actual production applications, and has a great application prospect in the fields of biological encoding and multiple biological analysis.

The invention relates to a preparation method of a chemical nanomaterial catalyst, and especially relates to a preparation method of an iron and nitrogen doped carbon nanoparticle photocatalyst. The method comprises the following steps: mixing materials: weighing 1.0g of a carbon source substance and 1.0g of a substance rich in amino groups, carboxyl groups and hydroxy groups, mixing the above substances in a 250mL beaker, adding 0.1g of iron trichloride, and adding a small amount of distilled water to dissolve above substances in order to obtain a purple solution; generating iron and nitrogen doped carbon nanoparticles: placing the solution in a constant temperature drying box, and heating at 210DEG C for 2h; and naturally cooling to obtain a brown-yellow foam solid in the beaker, wherein the solid is the iron and nitrogen doped carbon nanoparticles. The iron and nitrogen doped carbon nanoparticle solid is synthesized through direct co-heating with the carbon source substance and the substance rich in amino groups, carboxyl groups and hydroxy groups as precursors and iron trichloride as an iron source, so the method has the advantages of wide material sources, few synthesis steps, fast synthesis speed, no need of large scale devices, less investment, realization of macro production, and suitableness for industrial requirements.

The invention discloses a method for simultaneously labelling collagen type IV, macrophage and neovascularisation of tumors. The method comprises the following steps: 1. immobilizing tissue sections on a plus microscope slide processed by polylysine; 2. dewaxing the tissue sections in dimethylbenzene; 3. carrying out antigen retrieval; 4. washing the sections; 5. carrying out confining; 6. addingprimary antibodies; 7. washing the sections; 8. carrying out confining in the same way as the step 5; 9. using the fragment antigen-binding F(ab')2-QDs525 of goat anti-mouse immunoglobulin G labelledby quantum dot QDs525, the fragment antigen-binding F(ab')2-QDs585 of goat anti-rabbit immunoglobulin G labelled by quantum dot QDs585 and the fragment antigen-binding F(ab')2-QDs655 of rabbit anti-goat immunoglobulin G labelled by quantum dot QDs655 as secondary antibodies and dropwise adding the mixture after removing the confining liquid; 10. washing the sections; and 11. sealing the sections:preparing buffered glycerol with glycerol and 10ml of tris buffered saline (TBS) and storing the sections after sealing the sections with the buffered glycerol. The method is used for efficiently, accurately, rapidly and simultaneously detecting various components in tumor tissue microenvironment.

The invention relates to a method for preparing a high-brightness red light long afterglow luminous material. The method comprises the following steps: (1) dissolving a red fluorescent dye in deionized water, then adding a mixed solution of tetraethyl orthosilicate and absolute ethyl alcohol in deionized water, adjusting the pH value of the mixed solution to 2-3, and carrying out magnetic stirring for 20-30 minutes under conditions of the temperature of 60-70 DEG C and the stirring speed of 400 r/min, to form a uniform and transparent sol solution; (2) pouring a yellow green light long afterglow luminous material into the sol solution of the step (1), and carrying out magnetic heating stirring for 5-10 minutes, to form a flocculent gel; and (3) placing the flocculent gel of the step (2) in a room-temperature closed environment, aging for 20-24 h, then putting the aged product into a drying box, and drying for 20-24 h at the temperature of 60-80 DEG C; and then calcining the dried product for 3-4 h at the temperature of 300 DEG C in a calcining furnace, then taking out the calcined product, carrying out suction filtration of the calcined product with deionized water, and drying the product. The prepared red light long afterglow luminous material has the advantages of high fluorescent brightness, long afterglow time, no toxicity, no radioactivity, simple preparation process and the like.

The invention discloses a near-infrared IIb fluorescent probe which is based on a DPP (diketopyrrolopyrrole) conjugated polymer DT-R. The invention further discloses a preparation method of the fluorescent probe and nanoparticles of the fluorescent probe. The maximum emission peak of the prepared nanoparticles is located in the range of a near-infrared second region (NIR-II), and the longest emission wavelength of the prepared nanoparticles can extend to a near-infrared IIb region (NIR-IIb). The nanoparticle provided by the invention can realize NIR-IIb mouse blood vessel imaging under 808nm laser excitation, has high imaging resolution and signal-to-noise ratio, and has wide application prospects in the aspects of blood vessel monitoring, repairing and the like.

A thermochromic luminous cable comprises a plurality of conductors formed by twisting and an insulating shell outside the conductors. Flame-retardants are arranged between the conductors and the insulating shell in an extruding manner. The surface of the insulating shell is coated with a thermochromic layer. Raised spherical particles are uniformly distributed on the thermochromic layer, and the particles are of fluorescent materials. The thickness of the thermochromic layer is 15-30% of the diameter of the cable. The illumination brightness of the cable is greatly improved, an effective warning function is achieved, and the occurrence of accidents is reduced.

The invention relates to a preparation method of carbon-based nano particle multi-color fluorescent ink, and reates to a preparation method of fluorescent ink. According to the method, carbon-based nano particles are adopted as illuminants, a formula is regulated to enhancing illumination through carbon nano particles, the illumination color is changed through regulating the types of carbon nano points, and the carbon-based nano particle multi-color fluorescent ink is heated. The preparation method comprises the following steps: adding nano silicon dioxide, nano starch, nano protein powder, apreservative, polyethylene glycol 1500 and water into the fluorescent carbon nano particles, carrying out uniform ultrasonic stirring, and keeping the temperature at 60 DEG C for 2 hours to obtain thecarbon-based nano particle multi-color fluorescent ink. The fluorescent ink provided by the invention has wide raw material sources, simple synthesis method, simple required equipment and fast preparation speed; the obtained multi-color fluorescent ink is green and environmentally friendly and is convenient to carry and store; solid luminescence of fluorescent mark is realized; and the multi-color fluorescent ink is a good tool for advertisement, entertainment and teaching.

The invention belongs to the technical field of super-resolution microscopic imaging, and particularly relates to a fluorescent staining method for target protein of a DNA nano-structure labeled cell. Streptavidin is used as an intermediate, and a DNA nanostructure and an antibody of a biotin-modified target protein are connected together, so that the target protein is marked; and biotin, a group anchored on the hydrogel and more than two fluorescent dye molecules are simultaneously modified on the DNA nano structure. When the DNA nanostructure modifies the same dye, the brightness is very high, and the quality of the obtained microscopic picture can be greatly improved. When the dominant dye of the STED super-resolution microtechnique is modified, the expansion microtechnique can be well combined with the STED super-resolution technique. When a fluorescence resonance energy transfer dye pair is modified, the DNA nanostructure can have a flickering property, so that an expansion microtechnique is more conveniently combined with STORM and SOFI super-resolution techniques.

The invention discloses a water-carrying flow atomic fluorescence analysis device for analytical chemistry and an innovative analysis method, and relates to the field of water-carrying flow atomic fluorescence analysis. The water-carrying flow atomic fluorescence analysis device comprises an analyzer, the bottom of the analyzer is fixedly connected with a bottom plate, a limiting groove is formed in the upper wall of the bottom plate, and three clamping grooves are formed in the outer wall of the bottom plate at equal intervals; and clamping pins are rotationally connected to the middle parts of the three clamping grooves. Through the arrangement of the driving gear and the driven elliptical gear, when the inner walls of the three lamps face inwards synchronously and form a triangle, the lamps are excited to emit light, lamplight is fully irradiated into a reagent bottle, the three light sources are concentrated in the reagent bottle at the same time, the fluorescence brightness is improved, the three light sources are arranged in the triangle shape, scattering of light is prevented to a certain extent, and the practicability is improved; the fluorescence intensity contrast ratio is greatly increased through the setting of the step 3, the operation method is simple and convenient to operate, the adopted chemical reagent is low in cost, and the imaging effect of further imaging of atomic fluorescence is guaranteed.