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52results about How to "High fluorescence brightness" patented technology

System and method for quantitative detection of test strips on basis of continuous fluorescent-substance markers

ActiveCN101592659AEmitting characteristic wavelengths with high fluorescence brightnessHigh fluorescence brightnessBiological testingFluorescence/phosphorescenceAcquired characteristicFluorescence
The invention belongs to the field of bio-medical instruments, and in particular relates to a system and a method for quantitative detection of test strips on the basis of continuous fluorescent-substance markers. The system comprises a continuous fluorescent-substance marker test strip, a test strip frame, a lighting system, an imaging system, a fluorescent image receiver, a signal amplifier, an analog / digital converter, a data processing-controlling system, an output display device, a printer, a keyboard and an IC card matched with the test strip. The data processing-controlling system reads parameters of the IC card and then controls the test strip frame to move so as to ensure that the light emitted by the lighting system is reflected via a dichroic mirror and then automatically scans the test strip; acquired characteristic wavelength reflection fluorescence is transmitted to the data processing-controlling system for optical density identification and concentration calculation via the fluorescent image receiver, the signal amplifier and the analog / digital converter; and the output display device displays results. The invention can quickly and accurately realize the quantitative or qualitative detection of single-component and multi-component samples. The system has the characteristics of high detection sensitivity, objective results, flexible use and the like.
Owner:马义才

Multilayer fluorescent nanoparticles and methods of making and using same

InactiveUS20160018404A1Minimize energy transferNot decrease relative fluorescence emissionUltrasonic/sonic/infrasonic diagnosticsSurgeryMaterials scienceFluorescent nanoparticles
A multilayer, fluorescently responsive material (FRM)-containing nanoparticle and compositions comprising such nano-particles. The nanoparticles can be made using a layer-by-layer deposition method. The nanoparticles can be used in imaging methods such as, for example, cellular imaging methods.
Owner:CORNELL UNIVERSITY

Multi-shell-structure quantum dot composite particle, and high-fluorescent-brightness quantum dot probe and preparation method thereof

The invention discloses a multi-shell-structure quantum dot composite particle, and a high-fluorescent-brightness quantum dot probe and a preparation method thereof. The composite particle uses a quantum dot as a core, a semiconductor shell is coated outside the quantum dot, and a composite silicon dioxide shell is coated outside the semiconductor shell. A biological molecule can be connected to the composite silicon dioxide shell to form the probe. The composite particle and probe with required fluorescent wavelength can be obtained by controlling the reaction conditions. The method has strong operability. The obtained composite particle has favorable chemical and physical stability, biocompatibility and environmental stability. The formed probe has high stability and high fluorescent brightness, can have high application value in the fields of medicine and biology, and can be used as a common fluorescent probe, immunologic detection agent or any other biosensor.
Owner:UNIV OF JINAN

Chromophoric polymer dots

Owner:UNIV OF WASHINGTON CENT FOR COMMERICIALIZATION

Chromophoric polymer dots

Owner:UNIV OF WASHINGTON CENT FOR COMMERICIALIZATION

Method for dyeing fluorescent microballons

This invention relates to a method for dyeing polymer microspheres to obtain fluorescent polymer microspheres. The method comprises: uniformly mixing fluorescein 0.01-80%, polymer microspheres 0.1-80%, emulsifier 0-10%, tackifier 0-10%, and solvent (one or more of good solvents and poor solvents) 18.9-99.89%, dyeing under 1-101 kPa in dark for 1-720 h, taking out the dyed polymer microspheres, and washing repeatedly. The obtained fluorescent polymer microspheres have such advantages as simple process, high fluorescent brightness, wide fluorescent spectrum range, uniform particle size distribution and low relative standard deviation, and can be used as absolute counting microspheres of flow cytometry, fluoroimmunoassay microspheres, biosensor, microfluidic chip, and calibrator of fluorescence microscope.
Owner:TIANJIN UNIV

Method for fluorescent dyeing and finishing of cotton knit fabric

The invention discloses a method for the fluorescent dyeing and finishing of cotton knit fabric. The method comprises the following steps of: 1, pretreatment: soaking grey fabric in a bath tub filled with water soluble refining solution for refining; and neutralizing a pH value of the grey fabric to 6 to 7 by using aqueous solution of glacial acetic acid; 2, dyeing: soaking the refined grey fabric in a dye vat filled with water soluble dyeing solution for soaking, pickling the dyed colored fabric by using the aqueous solution of glacial acetic acid till the pH value of the washing solution is 4 to 5, and performing soaping and color fixing; and 3, post-finishing: taking the dyed colored fabric out of the dye vat, and soaking the dyed colored fabric in a reagent tank of a forming machine for forming. The method of the invention has the advantages of improving brightness and brilliance of the cotton fabric, improving the fluorescent color fastness of the pure cotton fabric to the fourth level or fifth level, and making the cotton fabric having certain depth and brilliance.
Owner:CHANGZHOU NEW WIDE KNITTING & DYEING

Chromophoric polymer dots

Owner:UNIV OF WASHINGTON CENT FOR COMMERICIALIZATION

Microfluidic-control preparation method for microsphere of polymethylmethacrylate-coated cadmium telluride (CdTe) quantum dot

InactiveCN102504822AUniform shapeConsistent and uniform sizeLuminescent compositionsInjectorQuantum dot
The invention relates to a microfluidic-control preparation method for a microsphere of a polymethylmethacrylate-coated cadmium telluride (CdTe) quantum dot, and relates to a preparation method for the microsphere of a polymethylmethacrylate-coated CdTe quantum dot. The microfluidic-control preparation method for the microsphere of the polymethylmethacrylate-coated (CdTe) quantum dot aims to solve the problems that the surface of the quantum dot needs to be modified and the quantum dot is easy to dissolve by the microsphere of the polymethylmethacrylate-coated CdTe quantum dot by adopting a traditional preparation method for the microsphere of the polymethylmethacrylate-coated CdTe quantum dot. The method comprises the following steps: preparing a sodium hydrogen telluride solution; preparing a CdTe quantum dot; preparing a polymer solution; connecting a microfluidic-control system; respectively putting the polymer solution and silicone oil into injectors A and B of the microfluidic-control system; setting the propulsion speed of the injectors A and B; after starting, formed liquid beads flow into a rotary evaporator; and curing and evaporating the silicone oil to obtain the microsphere of the polymethylmethacrylate-coated CdTe quantum dot. In the microfluidic-control preparation method for the polymethylmethacrylate-coated CdTe quantum dot, the polymethylmethacrylate-coated CdTe quantum dot is selected, therefore, fluorescence is stable, and the microsphere can be adjusted in size and can be used for field of fluorescent display and the like.
Owner:HEILONGJIANG UNIV

Water-dispersible multicolour fluorescent polymer nanoparticles and preparation method thereof

The invention discloses water-dispersible multicolour fluorescent polymer nanoparticles and a preparation method thereof. The water-dispersible multicolour fluorescent polymer nanoparticles are prepared by taking methyl methacrylate as a monomer, taking 4-ethyoxyl-9-allyl-1,8-naphthalimide (EANI), 4-amido-7-nitro-N-allylbenzo[1,2,5] oxadiazole (NBDAA), and Nile red methacrylate (NRME) as polymerizable fluorescent dyes, taking lauryl sodium sulphate (SDS) as a surfactant, and using a one-step miniemulsion polymerization method. The water-dispersible multicolour fluorescent polymer nanoparticles are great in dispersibility in water, quite high in fluorescent brightness, and capable of emitting multicolour fluorescence from blue to red under the excitation of a single wave. The water-dispersible multicolour fluorescent polymer nanoparticles obtained by the preparation method disclosed by the invention are uniform in size, and good in structure and light stability, has a fluorescence emission signal capable of being adjusted for many times in a wide interval under a single excitation wavelength, is simple in synthesis route, convenient to use, suitable for amplification synthesis and actual production applications, and has a great application prospect in the fields of biological encoding and multiple biological analysis.
Owner:HUNAN UNIV OF SCI & TECH

Fluorescent glaze and preparation method thereof

The invention provides a fluorescent glaze and a preparation method thereof. The fluorescent glaze is mainly prepared from the following raw materials by weight percentage: 50%-70% of glass frit, 20%-40% of feldspar and 2%-15% of calcined kaolin. The preparation method comprises the following steps of: firstly, mixing feldspar, calcium carbonate, barium carbonate, zinc oxide, aluminium oxide, borax, boric acid, quartz, strontium carbonate, vanadium pentoxide and bismuth oxide together in a certain weight ratio and then smelting the mixture at a temperature in the range from 1250 to 1350 DEG C; secondly, forming the glass frit after water quenching; and then enabling the glass frit to be subjected to coarse crushing, fine crushing and dosing grinding orderly, and mixing the ground powder with the feldspar and the calcined kaolin proportionally through a ball mill and grinding evenly, thereby obtaining the fluorescent glaze. The fluorescent glaze provided by the invention is free of rare-earth element, and can be excited under either natural light or a fluorescent lamp; the fluorescent glaze is high in fluorescent brightness and has persistence as long as 1300 min; the process of the preparation method of the fluorescent glaze is simple and capable of well meeting common requirements of the building ceramic industry.
Owner:美艺陶(福建)高新建材有限公司

Preparation method of iron and nitrogen doped carbon nanoparticle photocatalyst

The invention relates to a preparation method of a chemical nanomaterial catalyst, and especially relates to a preparation method of an iron and nitrogen doped carbon nanoparticle photocatalyst. The method comprises the following steps: mixing materials: weighing 1.0g of a carbon source substance and 1.0g of a substance rich in amino groups, carboxyl groups and hydroxy groups, mixing the above substances in a 250mL beaker, adding 0.1g of iron trichloride, and adding a small amount of distilled water to dissolve above substances in order to obtain a purple solution; generating iron and nitrogen doped carbon nanoparticles: placing the solution in a constant temperature drying box, and heating at 210DEG C for 2h; and naturally cooling to obtain a brown-yellow foam solid in the beaker, wherein the solid is the iron and nitrogen doped carbon nanoparticles. The iron and nitrogen doped carbon nanoparticle solid is synthesized through direct co-heating with the carbon source substance and the substance rich in amino groups, carboxyl groups and hydroxy groups as precursors and iron trichloride as an iron source, so the method has the advantages of wide material sources, few synthesis steps, fast synthesis speed, no need of large scale devices, less investment, realization of macro production, and suitableness for industrial requirements.
Owner:SHENYANG UNIV

Method for simultaneously labelling collagen type IV, macrophage and neovascularisation of tumors

The invention discloses a method for simultaneously labelling collagen type IV, macrophage and neovascularisation of tumors. The method comprises the following steps: 1. immobilizing tissue sections on a plus microscope slide processed by polylysine; 2. dewaxing the tissue sections in dimethylbenzene; 3. carrying out antigen retrieval; 4. washing the sections; 5. carrying out confining; 6. addingprimary antibodies; 7. washing the sections; 8. carrying out confining in the same way as the step 5; 9. using the fragment antigen-binding F(ab')2-QDs525 of goat anti-mouse immunoglobulin G labelledby quantum dot QDs525, the fragment antigen-binding F(ab')2-QDs585 of goat anti-rabbit immunoglobulin G labelled by quantum dot QDs585 and the fragment antigen-binding F(ab')2-QDs655 of rabbit anti-goat immunoglobulin G labelled by quantum dot QDs655 as secondary antibodies and dropwise adding the mixture after removing the confining liquid; 10. washing the sections; and 11. sealing the sections:preparing buffered glycerol with glycerol and 10ml of tris buffered saline (TBS) and storing the sections after sealing the sections with the buffered glycerol. The method is used for efficiently, accurately, rapidly and simultaneously detecting various components in tumor tissue microenvironment.
Owner:WUHAN UNIV

Detection kit for NPC (nasopharyngeal carcinoma) CTCs (circulating tumor cells)

The invention provides a detection kit for NPC (nasopharyngeal carcinoma) CTCs (circulating tumor cells). The detection kit comprises a human peripheral blood leucocyte removal part and an immunofluorescence in-situ hybridization identification part, wherein the human peripheral blood leucocyte removal part comprises a dilution buffer, a red blood cell lysis buffer, a density gradient centrifugation medium, a cell immobilization liquid A and immunomagnetic beads, and the red blood cell lysis buffer is formed by mixing ammonium chloride, sodium bicarbonate and an EDTA-2Na solution; the immunofluorescence in-situ hybridization identification part comprises a buffer, a hybridization stationary liquid B, a biotin labeled CEP8 probe, a red quantum dot labeled CD45 antibody, green quantum dot labeled streptavidin, a mounting medium and bovine serum albumin powder. The detection kit combines an immunomagnetic enrichment and separation technology, a fluorescence in-situ hybridization technology, an immunofluorescence cytochemical technology and an NPC CTC counting technology and can determine the number of the NPC CTCs in a sample precisely.
Owner:湖北省肿瘤医院

System and method for quantitative detection of test strips on basis of continuous fluorescent-substance markers

ActiveCN101592659BEmitting characteristic wavelengths with high fluorescence brightnessHigh fluorescence brightnessBiological testingFluorescence/phosphorescenceConvertersFluorescence
The invention belongs to the field of bio-medical instruments, and in particular relates to a system and a method for quantitative detection of test strips on the basis of continuous fluorescent-substance markers. The system comprises a continuous fluorescent-substance marker test strip, a test strip frame, a lighting system, an imaging system, a fluorescent image receiver, a signal amplifier, ananalog / digital converter, a data processing-controlling system, an output display device, a printer, a keyboard and an IC card matched with the test strip. The data processing-controlling system reads parameters of the IC card and then controls the test strip frame to move so as to ensure that the light emitted by the lighting system is reflected via a dichroic mirror and then automatically scansthe test strip; acquired characteristic wavelength reflection fluorescence is transmitted to the data processing-controlling system for optical density identification and concentration calculation via the fluorescent image receiver, the signal amplifier and the analog / digital converter; and the output display device displays results. The invention can quickly and accurately realize the quantitative or qualitative detection of single-component and multi-component samples. The system has the characteristics of high detection sensitivity, objective results, flexible use and the like.
Owner:马义才

Method for preparing high-brightness red light long afterglow luminous material

The invention relates to a method for preparing a high-brightness red light long afterglow luminous material. The method comprises the following steps: (1) dissolving a red fluorescent dye in deionized water, then adding a mixed solution of tetraethyl orthosilicate and absolute ethyl alcohol in deionized water, adjusting the pH value of the mixed solution to 2-3, and carrying out magnetic stirring for 20-30 minutes under conditions of the temperature of 60-70 DEG C and the stirring speed of 400 r / min, to form a uniform and transparent sol solution; (2) pouring a yellow green light long afterglow luminous material into the sol solution of the step (1), and carrying out magnetic heating stirring for 5-10 minutes, to form a flocculent gel; and (3) placing the flocculent gel of the step (2) in a room-temperature closed environment, aging for 20-24 h, then putting the aged product into a drying box, and drying for 20-24 h at the temperature of 60-80 DEG C; and then calcining the dried product for 3-4 h at the temperature of 300 DEG C in a calcining furnace, then taking out the calcined product, carrying out suction filtration of the calcined product with deionized water, and drying the product. The prepared red light long afterglow luminous material has the advantages of high fluorescent brightness, long afterglow time, no toxicity, no radioactivity, simple preparation process and the like.
Owner:JIANGNAN UNIV

Near-infrared IIb fluorescent probe, nano particle and preparation method and application thereof

The invention discloses a near-infrared IIb fluorescent probe which is based on a DPP (diketopyrrolopyrrole) conjugated polymer DT-R. The invention further discloses a preparation method of the fluorescent probe and nanoparticles of the fluorescent probe. The maximum emission peak of the prepared nanoparticles is located in the range of a near-infrared second region (NIR-II), and the longest emission wavelength of the prepared nanoparticles can extend to a near-infrared IIb region (NIR-IIb). The nanoparticle provided by the invention can realize NIR-IIb mouse blood vessel imaging under 808nm laser excitation, has high imaging resolution and signal-to-noise ratio, and has wide application prospects in the aspects of blood vessel monitoring, repairing and the like.
Owner:NANJING UNIV OF POSTS & TELECOMM

Thermochromic luminous cable

A thermochromic luminous cable comprises a plurality of conductors formed by twisting and an insulating shell outside the conductors. Flame-retardants are arranged between the conductors and the insulating shell in an extruding manner. The surface of the insulating shell is coated with a thermochromic layer. Raised spherical particles are uniformly distributed on the thermochromic layer, and the particles are of fluorescent materials. The thickness of the thermochromic layer is 15-30% of the diameter of the cable. The illumination brightness of the cable is greatly improved, an effective warning function is achieved, and the occurrence of accidents is reduced.
Owner:江苏海达电缆有限公司

Preparation method of carbon-based nano particle multi-color fluorescent ink

The invention relates to a preparation method of carbon-based nano particle multi-color fluorescent ink, and reates to a preparation method of fluorescent ink. According to the method, carbon-based nano particles are adopted as illuminants, a formula is regulated to enhancing illumination through carbon nano particles, the illumination color is changed through regulating the types of carbon nano points, and the carbon-based nano particle multi-color fluorescent ink is heated. The preparation method comprises the following steps: adding nano silicon dioxide, nano starch, nano protein powder, apreservative, polyethylene glycol 1500 and water into the fluorescent carbon nano particles, carrying out uniform ultrasonic stirring, and keeping the temperature at 60 DEG C for 2 hours to obtain thecarbon-based nano particle multi-color fluorescent ink. The fluorescent ink provided by the invention has wide raw material sources, simple synthesis method, simple required equipment and fast preparation speed; the obtained multi-color fluorescent ink is green and environmentally friendly and is convenient to carry and store; solid luminescence of fluorescent mark is realized; and the multi-color fluorescent ink is a good tool for advertisement, entertainment and teaching.
Owner:SHENYANG UNIV

Carborane-based hydrostatic pressure fluorescence sensing material as well as preparation method and application of hydrostatic pressure fluorescent sensing material

PendingCN113637028AIncrease brightnessOvercoming the problem of mechanofluorescence quenchingGroup 3/13 element organic compoundsFluorescence/phosphorescencePhenanthrolineBoronic acid
The invention discloses a carborane-based hydrostatic pressure fluorescence sensing material and a preparation method and an application thereof, the molecular formula of the material is C22H24B10, and the material is prepared through Suzuki coupling reaction by taking phenanthroline boric acid and 4-o-carborane bromobenzene as raw materials. The material can be used as a force-induced fluorescence discoloration pressure sensing probe, overcomes the problem of force-induced fluorescence quenching of a traditional material, shows a force-induced fluorescence enhancement property under low pressure, and has huge application potential in a pressure sensing system.
Owner:HUZHOU TEACHERS COLLEGE

Manufacturing method for lasting high-brightness fluorescent fishing rod

The invention discloses a manufacturing method for a lasting high-brightness fluorescent fishing rod. The manufacturing method comprises the following steps: manufacturing a blank fishing rod: uniformly mixing fluorescent powder and varnish according to a certain mass ratio to prepare a fluorescent paint; manufacturing an annular rubber baffle and mounting the annular rubber baffle on a paint pulling device; putting the fluorescent paint into a paint box of the paint pulling device; inserting one thin end of the blank fishing rod into a paint pulling hole from the paint box; rotating the paint pulling device to deposit the fluorescent paint on the blank fishing rod; pulling out the blank fishing rod from the paint pulling hole at the speed of 30cm-50cm per second and painting; and drying and assembling to obtain the lasting high-brightness fluorescent fishing rod. The fluorescent fishing rod manufactured by the method is long in luminous time and commonly can be luminous for eight to nine hours; the fluorescent brightness is high.
Owner:李占刚

Fluorescent staining method for target protein of DNA nano-structure labeled cell

The invention belongs to the technical field of super-resolution microscopic imaging, and particularly relates to a fluorescent staining method for target protein of a DNA nano-structure labeled cell. Streptavidin is used as an intermediate, and a DNA nanostructure and an antibody of a biotin-modified target protein are connected together, so that the target protein is marked; and biotin, a group anchored on the hydrogel and more than two fluorescent dye molecules are simultaneously modified on the DNA nano structure. When the DNA nanostructure modifies the same dye, the brightness is very high, and the quality of the obtained microscopic picture can be greatly improved. When the dominant dye of the STED super-resolution microtechnique is modified, the expansion microtechnique can be well combined with the STED super-resolution technique. When a fluorescence resonance energy transfer dye pair is modified, the DNA nanostructure can have a flickering property, so that an expansion microtechnique is more conveniently combined with STORM and SOFI super-resolution techniques.
Owner:FUDAN UNIV +1

Fluorescence Dyeing and Finishing Method of Cotton Knitted Fabric

The invention discloses a method for fluorescent dyeing and finishing of cotton knitted fabrics. The value is 6~7; ② Dyeing: Dip the refined gray cloth into the dye vat filled with water-soluble dye solution, and then pickle the dyed cloth with an aqueous solution of glacial acetic acid until the pH value is 4~5; Soaping and color fixing; ③Finishing: After the dyed cloth is taken out of the dyeing vat, it is dipped in the setting liquid in the medicine tank of the setting machine and then shaped. The method of the invention can improve the brightness and color fastness of cotton fabrics, raise the fluorescent color fastness of pure cotton fabrics to level 4 or level 5, and reach certain depth and color fastness.
Owner:CHANGZHOU NEW WIDE KNITTING & DYEING

Fluorescent yellow arc-proof fabric, application thereof and protective clothing

The invention provides a fluorescein arc-preventing fabric, application thereof and a protective garment, and relates to the technical field of arc-preventing fabrics, the fluorescein arc-preventing fabric comprises a surface fabric and an inner fabric which are stacked; wherein the surface-layer fabric and the inner-layer fabric are laminated and attached to form a whole through the connection of the connecting yarns; the surface-layer fabric is formed by weaving surface-layer yarns, and the surface-layer yarns comprise yarns obtained by blending modacrylic and flame-retardant nylon fibers; the inner-layer fabric is formed by weaving inner-layer yarns, and the inner-layer yarns comprise aramid fibers; the connecting yarns comprise yarns obtained by blending modacrylic, flame-retardant cellulosic fibers and aramid fibers. The technical problems that an existing fluorescein arc-proof fabric is thick and poor in breathability are solved, and the technical effects of being light, thin, comfortable, breathable, soft, good in flame-retardant effect and good in arc-proof function are achieved.
Owner:CHANGSHU BAOFENG SPECIAL FIBER

A kind of preparation method of carbon dot solid phosphor powder

InactiveCN105647529BRealize solid state light emissionEasy to prepareLuminescent compositionsOrganic acidPhosphor
The invention discloses a method for preparing carbon-dot solid-state fluorescent powder, and relates to a method for preparing fluorescent powder. The method includes particular steps of grinding amino compounds and organic acid, uniformly mixing the amino compounds and the organic acid with one another to obtain mixtures, transferring the mixtures into a constant-temperature tank, enabling the mixtures to react to one another at the temperatures of 120-210 DEG C for 1-5 hours to obtain a product and cooling the product to obtain brown foamy solid; ultrasonically dispersing solid powder in water to obtain fluorescent carbon-dot solution; adding an appropriate quantity of starch into the carbon-dot solution, ultrasonically dispersing the starch in the carbon-dot solution and then naturally drying the carbon-dot solution to obtain the carbon-dot solid-state fluorescent powder. The method has the advantages that raw materials for the carbon-dot solid-state fluorescent powder can come from wide sources, the method and required equipment for synthesizing the carbon-dot solid-state fluorescent powder are simple and are high in synthesis speed, the carbon-dot solid-state fluorescent powder is environmentally friendly, light can be emitted by fluorescent carbon dots in solid states, and the method is convenient to apply to the fields of photoelectric components, biological measurement, entertainment and the like.
Owner:SHENYANG UNIV

Water-carrying flow atomic fluorescence analysis device for analytical chemistry and innovative analysis method

The invention discloses a water-carrying flow atomic fluorescence analysis device for analytical chemistry and an innovative analysis method, and relates to the field of water-carrying flow atomic fluorescence analysis. The water-carrying flow atomic fluorescence analysis device comprises an analyzer, the bottom of the analyzer is fixedly connected with a bottom plate, a limiting groove is formed in the upper wall of the bottom plate, and three clamping grooves are formed in the outer wall of the bottom plate at equal intervals; and clamping pins are rotationally connected to the middle parts of the three clamping grooves. Through the arrangement of the driving gear and the driven elliptical gear, when the inner walls of the three lamps face inwards synchronously and form a triangle, the lamps are excited to emit light, lamplight is fully irradiated into a reagent bottle, the three light sources are concentrated in the reagent bottle at the same time, the fluorescence brightness is improved, the three light sources are arranged in the triangle shape, scattering of light is prevented to a certain extent, and the practicability is improved; the fluorescence intensity contrast ratio is greatly increased through the setting of the step 3, the operation method is simple and convenient to operate, the adopted chemical reagent is low in cost, and the imaging effect of further imaging of atomic fluorescence is guaranteed.
Owner:路桂娟

A kind of high-brightness, high-stability fluorescent dye excited by 488nm and its synthesis method

The invention provides a fluorescent dye with high brightness and high stability excited at 488nm and a synthesis method thereof. The fluorescent dye is a series of high brightness, high Stability Fluorescent dyes excited at 488nm, whose structural formula is shown in (1); Compared with existing commercial 488nm dyes FITC, Alexa488, etc., this series of dyes have higher photostability, and are sensitive to microenvironments such as pH, viscosity, temperature, and polarity. Both are insensitive and can maintain the accuracy of fluorescent signals in complex environments. The fluorescence half-peak width of this kind of dye is narrower in different solvents (<30nm), the fluorescence quantum yield in different solvents is greater than 0.80 (in water), and the molar extinction coefficient is greater than 4×10 4 m ‑1 cm ‑1 . The 488nm series dyes invented in this project are easy to be derived, and have good application prospects in the fields of fluorescence imaging and fluorescent labeling.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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