Detection kit for NPC (nasopharyngeal carcinoma) CTCs (circulating tumor cells)

Abstract

The invention provides a detection kit for NPC (nasopharyngeal carcinoma) CTCs (circulating tumor cells). The detection kit comprises a human peripheral blood leucocyte removal part and an immunofluorescence in-situ hybridization identification part, wherein the human peripheral blood leucocyte removal part comprises a dilution buffer, a red blood cell lysis buffer, a density gradient centrifugation medium, a cell immobilization liquid A and immunomagnetic beads, and the red blood cell lysis buffer is formed by mixing ammonium chloride, sodium bicarbonate and an EDTA-2Na solution; the immunofluorescence in-situ hybridization identification part comprises a buffer, a hybridization stationary liquid B, a biotin labeled CEP8 probe, a red quantum dot labeled CD45 antibody, green quantum dot labeled streptavidin, a mounting medium and bovine serum albumin powder. The detection kit combines an immunomagnetic enrichment and separation technology, a fluorescence in-situ hybridization technology, an immunofluorescence cytochemical technology and an NPC CTC counting technology and can determine the number of the NPC CTCs in a sample precisely.

Description

technical field [0001] The invention provides a detection kit for circulating tumor cells of nasopharyngeal carcinoma, which belongs to the field of detecting circulating tumor cells of nasopharyngeal carcinoma. Background technique [0002] Circulating Tumor Cells (CTCs) refer to tumor cells that enter the blood. These tumor cells participate in the blood circulation, and can migrate to related tissues or organs along the direction of blood circulation, and develop into tumor lesions under suitable conditions. The main sources of CTCs are divided into two aspects: one is shedding, that is, shedding from the primary tumor or metastases, penetrating the blood vessel wall and entering the blood. The second is entry at an earlier stage, that is, before the formation of imaging-visible tumor lesions, it enters the blood in the form of cancerous cells in advance. discovery. [0003] Common examination methods for nasopharyngeal carcinoma include: magnetic resonance imaging exa...

AI Technical Summary

Problems solved by technology

The current detection method generally has the following defects: due to the low removal rate of leukocytes in peripheral blood, leukocytes as nucleated cells will interfere with the enrichment of tumor cells

In addition, blood-borne abnormal cells can also interfere with the selection of non-blood-derived abnormal cells (circulating tumor cells)

In addition, most of the current positive sorting methods are used to capture circulating tumor cells, and specific antibodies (such as Epcam, CK19, etc.) coupled to the surface of magnetic beads are used to capture tumor cells. The cells obtained by this method have magnetic beads on the surface, resulting in cell viability reduce

And due to the relationship between markers, the recovery rate of tumor cell enrichment is low

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