119 results about "Lysis buffer" patented technology

The invention discloses a preparation method and application of a gynostemma pentaphylla exosome. According to the method, a high-activity callus is obtained by optimizing a callus induction culture medium, and a suspension culture system is established to realize biomass accumulation with the dry cell weight of 19.8 + / -0.7 g / L. A sequential progressive composite induction strategy (methyl jasmonate is added after chitosan is pre-induced for 24 hours) is innovatively adopted, so that the saponin content is remarkably increased to 102.6 + / -4.8 mg / g DW. Exosomes (P-Exos) are extracted from induced cells through an enzymolysis-magnetic bead affinity purification technology (WGA modified magnetic bead affinity capture after mild enzymolysis of a cracking buffer solution), the yield reaches 1.45 + / -0.08 mg / g cells, and the extraction efficiency is remarkably improved compared with that of a traditional gradient centrifugation method (C-Exos). Moreover, the gynostemma pentaphyllum exosome prepared by the invention can effectively improve the synthesis of skin collagen, has a better effect, can be applied to medicines for promoting skin wound repair or can be added into anti-aging cosmetics as a functional component, and has a very high application value.

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

The invention belongs to the technical field of biomolecular interaction detection, and particularly relates to a method for synergistically analyzing a molecular interaction state based on multimodal optical parameters of a rare earth up-conversion nano material. The method comprises the following steps: S1, providing a ligand coated rare earth up-conversion nano material UCNPs probe; s2, enabling the rare earth up-conversion nano material UCNPs probe to be in contact with a solution sample to be detected; s3, exciting the rare earth up-conversion nano material UCNPs probe by using near-infrared pulse laser, and collecting the following optical parameters: luminous intensity change, molecular layer thickness / mass change delta D, luminous life change delta tau and emission peak displacement delta lambda; and S4, based on at least two of the parameters, cooperatively analyzing and judging the interaction state of the ligand on the surface of the probe and the molecules in the solution sample to be detected. The molecular interaction state is synergistically analyzed based on changes of multiple optical parameters, and the technical problem that an existing molecular interaction analysis technology cannot be applied to complex biological samples (serum and cell lysis buffer) is solved.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention discloses a method and a device suitable for CUAMP. The invention discloses a method for extracting liriodendron tulipifera cell nucleuses by a Tag technology and application of the liriodendron tulipifera cell nucleuses. Hybridized liriodendron tulipifera calluses are used as materials, and protoplasts are released through enzymolysis solution vacuumizing and mild enzymolysis for 1.5 h; the method comprises the following steps: sequentially purifying by using a W5 solution and mannitol, detecting the activity by FDA, cracking by using an NE buffer solution, and washing by using a Wash buffer solution in two steps, thereby obtaining 2 * 10 < 6 >-3 * 10 < 6 > high-purity and high-integrity cell nucleuses. The obtained core is clean in background and complete in membrane structure, and can be directly used for CUTamp; tag is used for building a library, so that the magnetic bead capturing efficiency is improved by 46%, and protein-DNA interaction high-throughput sequencing under the condition of low sample size is realized. The method disclosed by the invention is simple and convenient to operate and good in repeatability, and provides key technical support for epigenetic research of rare tree species such as liriodendron tulipifera.

The invention discloses a method for accurately identifying and screening morchella strains by using molecular markers, and belongs to the technical field of edible mushrooms. The method comprises the following steps: transferring a purified morchella strain to a culture medium for dark light cultivation; adding the cultured morchella hyphae into liquid nitrogen, grinding into powder, then adding a lysis solution, and carrying out lysis; adding modified magnetic beads into the lysate for adsorption, and then filtering, washing and eluting to obtain a DNA extracting solution; carrying out PCR (Polymerase Chain Reaction) amplification on the DNA; then carrying out high-resolution detection on an ISSR product; and then, carrying out functional SNP-KASP typing. According to the method, by fusing ISSR polymorphism analysis and a functional SNP-KASP typing technology, magnetic beads are used for adsorbing DNA to remove polysaccharide interference, and on the basis that the low cost advantage of traditional ISSR is reserved, three functional markers are completed, and accurate screening of yield, heat resistance and flavor is achieved.

The present invention provides a cfDNA extraction kit and method. The cfDNA extraction kit provided by the present invention includes proteinase K, a lysis buffer, magnetic microbeads, and a binding buffer. By limiting the composition of the lysis buffer and the binding buffer, it can not only effectively improve the extraction efficiency of cfDNA, but also significantly increase the purity of cfDNA and effectively enrich cfDNA of 100-200 bp. In addition, the kit components are easily available and low-cost, and the extraction operation is simple and suitable for high-throughput processing.

The invention relates to a method for determining enzyme activity of ODC1 (ornithine decarboxylase 1), which comprises the following steps: digesting, centrifuging and resuspending cells, and taking a part of sample to detect protein concentration; the method comprises the following steps: cracking cells by using a specially-made enzyme activity cell lysis solution and a protein lysis solution, and ensuring full cracking through ultrasonication; carrying out quantitative analysis on the protein in the lysate by adopting a BCA kit; in the enzyme activity detection stage, a cell lysis solution and an enzyme activity detection working solution containing a specific fluorescent probe (CB6) are mixed and react at 37 DEG C for a certain time, and then the change of fluorescence intensity is determined by a multifunctional fluorescence microplate reader, so that the enzyme activity of the ODC1 is evaluated. According to the method, accurate determination of the ODC1 enzyme activity is realized by optimizing cell treatment, protein quantification and enzyme activity detection processes. The method has the advantages of simplicity and convenience in operation, high sensitivity, good specificity and the like, and is suitable for the fields of basic research, drug screening and the like related to the ODC1 enzyme activity.

The invention belongs to the technical field of molecular biology, and particularly relates to a pathogenic microorganism nucleic acid co-extraction kit, an extraction method and application. The invention provides a lysis solution for nucleic acid co-extraction of pathogenic microorganisms. The lysis solution comprises a first guanidine salt, an ionic surfactant, a first sodium salt and a first nonionic surfactant. According to the splitting system, guanidine salt is adopted and matched with a certain content of sodium salt and a surfactant, a sample is split and precipitated under the grinding condition, nucleic acid cannot be damaged in the crushing process, and pathogenic microorganism DNA and RNA with high concentration and complete fragments can be extracted.

The invention discloses a kidney cancer detection method based on BAFF / TACI interaction molecules and a kit thereof, and the method comprises the following steps: S1, sample pretreatment: collecting a serum or tissue supernatant sample, carrying out centrifugal separation on the serum for 10 min under the conditions of 3000 rpm and 4 DEG C, adding an RIPA lysate into the tissue sample for homogenization, then carrying out centrifugation for 15 min under the conditions of 12000 rpm and 4 DEG C, and taking the supernatant; diluting the sample with PBS (phosphate buffer solution) containing 0.1% of BSA (bovine serum albumin); s2, constant-temperature three-channel ELISA detection: adopting a pre-coated three-channel 96-hole detachable elisa plate (7), and synchronously detecting in a constant-temperature environment of 37 + / -0.5 DEG C; S3, result judgment: the critical values are sBAFF > = 85pg / mL, sTACI > = 42pg / mL, and BAFF-TACI complex > = 12pg / mL, and if any condition is met, the kidney cancer is positive, and the kit has the advantages that the kit is suitable for early screening, clinical diagnosis, pathological staging and prognosis evaluation of the kidney cancer, and has a good application prospect. Through constant temperature control and three-channel independent hole design, synchronous and accurate detection of BAFF, TACI and BAFF-TACI compounds in various samples such as serum, plasma, tumor tissue and the like is realized.

The invention discloses a method for extracting cell nucleuses from animal tissues, and relates to the technical field of biology. The background technology indicates that cell nucleus extraction is crucial to multidisciplinary research, but a traditional method has the problems of complex operation, time consumption, low purity, easiness in damaging cell nucleuses and the like, and the prior art is still limited although the prior art is improved. The method comprises the following steps: preparing a sample, and taking fresh or cryopreserved animal tissues; homogenizing the tissues, namely putting the tissues into a homogenizer, and homogenizing at a low speed by adding a cracking buffer solution; carrying out cell lysis, and incubating homogenate on ice; centrifugally separating, centrifuging at low temperature and medium speed, taking precipitate, and discarding supernatant; core washing, re-suspending the precipitate in a washing buffer solution, uniformly mixing and filtering; performing core extraction, re-suspending the precipitate in a suspension buffer solution, and lightly and uniformly mixing; purifying and washing, carrying out density gradient centrifugation on the suspension, dyeing with trypan blue, taking a core layer, and washing for subsequent experiments. The nuclear extraction lysis buffer solution has two proportions of LB1 and LB2, different tissues are suitable for different proportions, and an enzyme inhibitor can be added as required. The method can be used for rapidly extracting various animal tissue cell nucleuses, is complete in shape, large in quantity and few in impurities, is suitable for different tissues, and can be applied to downstream experiments such as single cell nucleus sequencing.

This invention discloses a kit and method for extracting cell nuclei from plant tissues, belonging to the field of biotechnology. The kit includes a nuclear lysis buffer and a nuclear washing buffer. The nuclear lysis buffer is prepared using nuclease-free water with sucrose, Tris-HCl (pH 7-8), NaCl, MgCl2, a surfactant, DTT, an RNase inhibitor, and spermidine. The nuclear washing buffer is prepared using nuclease-free water with sucrose, Tris-HCl (pH 7-8), NaCl, MgCl2, DTT, an RNase inhibitor, and spermidine. This kit is suitable for extracting cell nuclei from fresh yew stem tissue, yielding cell nuclei with intact nuclear membranes, stable gene expression, high purity, and few impurities, suitable for single-cell sequencing.

The invention relates to the technical field of nucleic acid detection, in particular to a lysis buffer solution, a kit and an extraction method for extracting free DNA of peripheral blood. The lysis buffer solution for extracting the free DNA of the peripheral blood comprises a first lysis solution and a second lysis solution, the first lysis solution takes guanidine hydrochloride as a main chaotropic agent and at least one of ammonium thiocyanate and guanidine isothiocyanate as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate and ascorbic acid; the second lysis solution takes guanidine hydrochloride as a main chaotropic agent and urea as an auxiliary chaotropic agent, and is compounded with a metal ion chelating agent, trehalose, dithiothreitol, digestive enzyme, sodium dodecyl sarcosinate, borate, ascorbic acid and polyethylene glycol. The buffer solution of the lysate does not contain SDS (sodium dodecyl sulfate), so that the residue of SDS in an extracted product can be avoided, and the amount of extracted free DNA (deoxyribonucleic acid) is more.

This invention relates to the field of peptide preparation technology, specifically to a preparation process for terfenadine. The terfenadine preparation process includes the following steps: (1) using resin as a solid-phase carrier, Boc-Pip(Fmoc)-OH is loaded onto the resin to obtain Boc-Pip(Fmoc)-resin; (2) under the action of a condensing agent, Fmoc-D-Lys(Boc)-OH and Boc-D-Phe-D-Phe-D-Leu-OH are sequentially coupled onto the Boc-Pip(Fmoc)-resin to obtain terfenadine peptide resin; (3) the terfenadine peptide resin is lysed using a lysis buffer, then concentrated, precipitated, and dried to obtain crude terfenadine. This invention's preparation process can effectively improve product purity while ensuring large-scale industrial production, and the steps are simple with a short reaction cycle.

The invention relates to the technical field of virus extraction kits, in particular to a large-volume sample virus nucleic acid rapid extraction kit and method.The large-volume sample virus nucleic acid rapid extraction kit comprises a protease K solution, magnetic beads, a lysis solution, a rinsing solution 1, a rinsing solution 2 and an eluent; the magnetic beads are hydroxyl magnetic beads; the lysate is prepared from guanidine isothiocyanate, Tris alkali, Tris hydrochloric acid, triton X-100, isopropyl alcohol and polyoxyethylene ether; the rinsing liquid 1 is composed of a guanidine hydrochloride solution and isopropanol; the rinsing liquid 2 is composed of absolute ethyl alcohol and purified water; the kit provided by the invention can easily obtain viral nucleic acid from a large-volume sample, is simple to operate, can extract a plurality of samples at one time, realizes rapid diagnosis, and makes up the vacancy of a kit for automatically extracting viral nucleic acid from a large-volume sample at high flux in the current market.

The present invention relates to a method for isolating and enriching pathogenic nucleic acids from a biological sample thereby reducing host nucleic acid contamination and enrich relative pathogenic DNA in the infectious disease sample and a device thereof. The method involves mixing pathogenic nucleic acid containing biological sample with a lysis buffer at a temperature between 25-35°C for partial or incomplete lysis of the sample; passing the lysed sample through a depth filter wherein the filter paper size ranges from 0.22 microns to 20 microns; passing an elution buffer by reverse plunging action and generating a vibration of 200 to 350 Hz with discontinuous pulse of 20-30 seconds for 2-5 minutes for eluting pathogenic nucleic acids into the elution buffer. The invention further provides a device for isolating and enriching pathogenic nucleic acids from a nucleic acid containing biological sample thereby reducing host nucleic acid contamination using said method.

This invention provides an oligonucleotide cleavage reagent (JS2 & S4 reagent) and its preparation method, as well as a method for cleaving oligonucleotides. The oligonucleotide cleavage reagent comprises NaOH, KOH, methanol, and a cleavage buffer. The oligonucleotide cleavage reagent JS2 & S4 and the cleavage method using it provided by this invention can efficiently cleave oligonucleotides, especially those synthesized using solid-phase phosphorous acid cleavage. This reduces chain breakage and side reactions caused by high-temperature cleavage, and also reduces equipment costs. While shortening the cleavage time, it can effectively reduce oligonucleotide quality problems caused by cleavage.

The invention belongs to the technical field of biomedical detection, and relates to a colon cancer stem cell marker detection kit. The kit comprises a processing assembly, a detection assembly, a reading assembly and a shell, a detection strip is arranged in the detection assembly, and three markers of CD133, LGR5 and ALDH1 are simultaneously detected by adopting a lateral flow immunochromatography technology; the treatment assembly comprises a sample collector, a cracking buffer solution storage cavity and a mixing cavity, and can automatically complete sample pretreatment; the reading assembly comprises an optical detection module and a signal processing module and can quantitatively analyze the detection result. The kit has the advantages of simplicity and convenience in operation, rapidness in detection, high precision, high portability and the like, the detection time is 15-30 minutes, the detection limit can reach the ng / mL level, and the kit is suitable for clinical rapid detection and basic medical institutions and provides technical support for early diagnosis and treatment monitoring of colon cancer.

The application provides a plasmid DNA extraction kit based on an anion exchange column and application thereof, and relates to the technical field of biology.The plasmid DNA extraction kit based on the anion exchange column comprises an anion exchange column, a lysis buffer group, a balance buffer, a washing buffer and an elution buffer; the anion exchange column comprises a solid-phase extraction empty column, upper and lower sieve plates arranged on the inner side of the empty column and located at the bottom of the empty column, and an anion exchange ligand filled between the upper and lower sieve plates; the anion exchange ligand comprises diethylaminoethyl dextran gel DEAE-A50 and diethylaminoethyl cellulose DE52.The application uses domestic fillers and optimizes the ratio of the fillers as the anion exchange ligand, the cost of which is only 1 / 20-1 / 40 of that of imported products, and the operation time and labor intensity are saved through the gravity flow purification process, and the obtained plasmid has high yield, and the transfection effect is equivalent to that of commercially available kits.

The invention belongs to the technical field of animal epidemic disease detection, and particularly relates to an animal epidemic disease high-sensitivity field detection system combining CRISPRCas and digital PCR, a microfluidic digital PCR-CRISPR integrated chip comprises a nucleic acid extraction area and a digital PCR amplification area which are communicated in sequence; the nucleic acid extraction area is pre-coated with magnetic beads and a lysis buffer solution, the digital PCR amplification area is pre-sub-packaged with an animal epidemic disease target specific primer pair, a fluorescent probe and a PCR premix solution, and the CRISPR verification area is pre-coated with crRNA, Cas12a protein and ssDNA fluorescent report probes aiming at a target sequence; the matched detection kit comprises a sample lysis solution, a quality control standard substance, a positive plasmid and a negative control, and the portable fluorescence detection reader can simultaneously read a fluorescence quantitative signal of the digital PCR amplification region and a fluorescence report signal of the CRISPR verification region and automatically output a detection result. The CRISPR verification of the invention further eliminates false positive; reagents are prepackaged in the chip, professional equipment is not needed, and detection is completed within 30 minutes; and at least four animal epidemic disease pathogens can be detected at the same time.

The invention discloses a single cell whole genome methylation and transcriptome biomics library construction method, and relates to the technical field of sequencing methods.The method comprises the steps that a single cell is added into a sorting tube with a preset lysis buffer for lysis, a nucleophilic coating is arranged on the inner wall of the sorting tube to enable cell nuclei to be adsorbed, and supernate containing mRNA is obtained; carrying out reverse transcription on supernate to obtain cDNA (complementary deoxyribonucleic acid) and constructing a transcriptome library; splitting the cell nucleus to release DNA (Deoxyribonucleic Acid) and carrying out hydrosulfite conversion; the transformed DNA is connected with a molecular tag-containing joint to obtain a methylated intermediate product, after exonuclease treatment, the intermediate products with different molecular tags are combined, and the methylated library is obtained through magnetic bead purification, single chain, connection with a sequencing joint at the other end, amplification and purification. According to the technical scheme, RNA and DNA are separated in the same tube and a library is established in parallel, separation and transfer steps are reduced, RNA degradation and nucleic acid loss are reduced, and methylation library establishment flux and biomics data consistency are improved.

This invention discloses a single-cell microarray-based kit and method for in situ quantitative detection of receptor-binding allergen-specific IgE. The kit includes: a single-cell microcavity array chip, an antigen barcode chip, a plastic clamp, IgE standards, fluorescently modified IgE detection antibodies, and auxiliary reagents. During detection, basophil single cells are loaded into the microcavity array chip, lysis buffer is added, and the cells are clamped together with the antigen barcode chip. Low-temperature incubation allows the allergen-specific IgE released from cell lysis to be captured in situ by the antigen on the chip. A sandwich immunoassay is then performed using a fluorescently labeled antibody, and absolute quantification is achieved using a standard curve. This invention is the first to achieve high-throughput, high-sensitivity, multi-component parallel in situ quantitative detection of basophil membrane receptor-binding sIgE at the single-cell level. It can directly reflect the functional sensitization state of effector cells and has advantages such as simple operation, low sample requirement, and no need for complex valve devices.

This invention provides a lysis buffer for nucleic acid extraction and its application. The lysis buffer comprises: 0.6M–0.8M NaH₂PO₄, 0.042–0.06M Na₂HPO₄, 0.007–0.009M NaCl, 0.03–0.05M Tris, 0.01–0.02M SDS, and 10%–20% anhydrous ethanol; wherein the pH of the lysis buffer is 5–6; and the nucleic acid is DNA and / or RNA. Using the lysis buffer of this invention, the sample can be efficiently lysed, effectively releasing and protecting the nucleic acid molecules in the sample, significantly improving the nucleic acid extraction efficiency. This invention provides a new reference for extracting DNA and / or RNA from low-biomass samples, contributing to improved sequencing quality of low-biomass samples.

The invention discloses an injector-based nucleic acid purification device, an extraction method and a viral nucleic acid lysate. The purification device comprises an injector assembly and a filter membrane assembly, wherein the injector assembly comprises an injector tube barrel and a push rod matched with the injector tube barrel; the filter membrane assembly is connected to the outlet end of the injector tube barrel, and the filter membrane assembly is configured to be of a detachable structure. The nucleic acid purification device provided by the invention perfects the processes of nucleic acid release, nucleic acid washing and elution, can remove components which interfere with subsequent PCR (Polymerase Chain Reaction), fluorescent quantitative PCR and constant-temperature detection in the nucleic acid release process, and can improve the detection efficiency; the viral nucleic acid lysate is suitable for common clinical samples such as nasal swabs, anus swabs, serum and ascites, and is suitable for nucleic acid extraction and purification of DNA and RNA viruses by optimizing the proportion of guanidine isothiocyanate, polyvinylpyrrolidone (PVP-40) and lauryl sodium sulfate (SDS).

The invention discloses a cell lysis solution of peripheral blood mononuclear cells and application of the cell lysis solution in arthritis treatment, and belongs to the technical field of medicine preparation.The cell lysis solution of the peripheral blood mononuclear cells is prepared by the steps that gardenia powder is hydrolyzed to prepare gardenia jasminoides hydrolysate, the gardenia jasminoides hydrolysate and PBMC cells are co-cultured for anti-inflammatory induction, and the cell lysis solution of the peripheral blood mononuclear cells is obtained. And carrying out homogeneous lysis on the induced PBMC cells to obtain a PBMC lysis buffer, and mixing the PBMC lysis buffer with trehalose dihydrate, strontium chloride, selenomethionine and astaxanthin to prepare the peripheral blood mononuclear cell lysis buffer. According to the composition disclosed by the invention, the lasting exertion of the drug effect is ensured, the swelling and pain symptom of a patient can be quickly relieved after injection, the immediate treatment effect and the compliance of the patient are improved, and the technical problems that the traditional water injection is quickly cleared in an articular cavity and the effectiveness time is short are solved.

This invention relates to the field of nucleic acid detection technology, specifically disclosing a liquid-phase nucleic acid chip for isothermal amplification of common algal blooms and its application. The method includes: collecting water samples and filtering to enrich algal cells; lysing the algal cells using cell lysis buffer to extract DNA; adding the extracted DNA to the liquid-phase nucleic acid chip for isothermal amplification; detecting the Ct value using a qPCR instrument; and determining the species and cell density of the algal bloom based on a preset standard curve. This invention directly quantifies algal cell density using algal gene copy number, with significantly higher accuracy than microscopic counting, and does not rely on the experience of professional personnel. It can be widely applied in frontline water resource management work.

The invention discloses a G protein coupled receptor family membrane receptor GPR40 related to eukaryotic expression and glycometabolism effect, and a method for extracting and detecting the concentration of a compound combined with the GPR40 receptor in a cell based on the affinity of receptor protein. The detection method comprises the following steps: eukaryotically expressing a membrane receptor GPR40 protein with a Flag tag in a human embryonic kidney cell HEK293, and after perfluorooctane sulfonic acid (PFOS) is exposed, extracting and purifying a GPR40-PFOS compound in a cell lysis solution by utilizing Protein G magnetic beads and a Flag antibody; after uncombined PFOS is washed away by a cracking buffer solution containing a protease inhibitor, acetonitrile is added, and PFOS in the compound is extracted; and centrifuging and treating with a 0.22 mu m filter membrane, and detecting the concentration of the PFOS by using a high performance liquid chromatography-mass spectrometer (HPLC-MS / MS). The method can directly detect the concentration of PFOS combined with the GPR40 receptor, can be used for screening other perfluorinated compounds and environmental pollutants which potentially act on the GPR40 receptor to play a glycometabolism effect, can simplify the steps of effect-oriented analysis, and plays an important role in compound recognition and screening.

The application provides a preparation kit for a Puccinellia distans cell nucleus suspension and relates to the field of molecular biology, and comprises a lysis buffer system, a phase separation system, a density gradient purification system and a lysis and staining system; wherein the lysis buffer system comprises 1xNIB working solution and 1xTAPS resuspension buffer, the pH value of the 1xTAPS resuspension buffer is 7.8-8.2; the phase separation system adopts ATPS two-phase separation solution; the density gradient purification system adopts 33%-37% Percoll separation solution; and the lysis and staining system comprises a cell lysis solution and a DAPI staining solution. The kit can obtain stable high-quality plant cell nucleus suspension, makes the cell nucleus damage small, improves the nucleus integrity rate, reduces the cell nucleus fragments and reduces the cell nucleus adhesion, and provides key technical support for plant single cell omics research of Puccinellia distans and the like.

The invention discloses a compound gel preparation for treating osteoarthritis as well as a preparation method and application thereof, relates to the field of biological medicines, and aims to solve the problems of low number of anti-inflammatory and repairing factors, few types and non-lasting effect in the existing multi-factor product for treating osteoarthritis. The compound gel preparation comprises a platelet-derived anti-inflammatory repair factor and a vesicle lysate. The method comprises the following steps: 1, adding schisandrin B into blood to prepare platelet-rich plasma, and performing freeze thawing treatment to prepare platelet-derived anti-inflammatory repair factors; 2, inducing the bone marrow mesenchymal stem cells by using an induction solution added with 18 beta-glycyrrhetinic acid; separating to obtain extracellular vesicles, and carrying out ultrasonic lysis to obtain a vesicle lysis solution; 3, mixing the platelet-derived anti-inflammatory repair factor with the vesicle lysis buffer to obtain a joint repair factor; and 4, mixing the joint repair factor with the chitosan-poloxamer temperature-sensitive gel to obtain the compound gel preparation. The compound gel preparation is used for treating osteoarthritis.

This invention relates to the field of fecal genomics kit technology, and particularly to a safe, rapid, and high-throughput kit for extracting fecal genomes and its usage method. The kit includes a sample preservation solution, magnetic beads, a lysis buffer, a washing buffer 1, a washing buffer 2, and an elution buffer. The sample preservation solution is composed of guanidine isothiocyanate, sodium citrate, Triton X-100, Tween 20, propidium iodide, and purified water. The magnetic beads are hydroxyl magnetic beads. The lysis buffer is composed of guanidine hydrochloride, Tris base, Tris hydrochloric acid, Triton X-100, isopropanol, and purified water. Washing buffer 1 is composed of guanidine hydrochloride solution and isopropanol. Washing buffer 2 is composed of anhydrous ethanol and purified water. This kit can easily extract the genome from fecal samples, is simple to operate, safe and non-toxic, and can extract multiple samples at once, enabling rapid diagnosis and filling the gap in the current market for automated high-throughput kits for extracting genomes from fecal samples.