71 results about "Molecular switch" patented technology

The invention relates to the technical field of biology, in particular to a molecular switch system with a signal cascade amplification function and application of the molecular switch system. The invention provides a molecular switch of a signal cascade method and a gene expression or regulation system. The molecular switch comprises an activator protein araC gene, a bidirectional pTet promoter, a repressor protein tetR gene, a pBad promoter and a tetO operon which are connected from upstream to downstream. When the molecular switch is used for expression or silencing of a target gene, lower background leakage, higher response rate, larger expression range and higher expression intensity are achieved, and expression is more homogeneous; and the inhibition efficiency is obviously improved.

The invention discloses a colorimetric aptamer sensor and application thereof, and belongs to the technical field of biological detection. The colorimetric aptamer sensor comprises Cas12a protein, HP, crRNA, dsDNA (double-stranded deoxyribonucleic acid) and a colorimetric probe. The colorimetric biosensor is applied to detection of the ochratoxin A, the content of the ochratoxin A is detected through the colorimetric biosensor, and detection and analysis of a target object can be achieved; cas12a aptamer inhibitors are developed as molecular switches for biosensors, which mediate target recognition through engineering modification of HP hairpin sequences. A new thought for improving the mycotoxin detection sensitivity is explored, and good detection potential is expected to be shown in the field of food safety.

The present invention relates to a modified stem cell and an ex vivo method for preparing defined mature cells from genetic programming of said modified stem cell which can be carried out in absence of certain growth factors, as well as a kit allowing to transform stem cells, and the different uses of the mature cells, for example for in vitro drug screening and toxicology or as cultivated meat. The genetic programming involves a molecular switch between two mutually exclusive programs of stem cell expansion and differentiation. The invention may be used for stem cells or other progenitors cells of any type, from any eukaryotic organism, but finds particular application in pluripotent stem cells from humans and livestock animals.

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.

The invention provides a DNA enzyme triple-helix molecular switch detection system adaptive to a personal glucometer as well as a preparation method and application of the DNA enzyme triple-helix molecular switch detection system, and belongs to the technical field of biosensors. The DNA enzyme triple-helix molecular switch disclosed by the invention comprises an aptamer containing two nucleotide arm segments and DNA enzyme covalently coupled with a magnetic bead, two nucleotide arm segments of the aptamer and DNA enzyme form a triple helix structure; the two nucleotide arm segments comprise a nucleotide sequence SEQ ID NO.1 at the 5'end of the aptamer and a nucleotide sequence SEQ ID NO.2 at the 3 'end of the aptamer. According to the invention, the flexibility of an original aptamer is limited by using a terminal fixation strategy, the spatial conformation of the original aptamer is stabilized, the affinity of the aptamer to a target is improved, and the sensitivity of the aptamer to the target is improved by using a triple-helix molecular switch containing DNA enzyme and nano-enzyme-gold nanoparticles. Accurate and sensitive detection of trace pollutants in food is realized by measuring the reduction amplitude of the glucose concentration in the detection liquid through a household glucometer.

The invention designs a preparation method of an electrochemical sensor for detecting acrylamide based on a Ce-MOF and AuPd nanoflower composite material. The advantages of large surface area and good stability of Ce-MOF are utilized to load a large amount of AuPd NFs, and the composite material Ce-MOF (at) AuPd NFs with high conductivity is obtained. The preparation method comprises the following steps: modifying an electrode by virtue of a physical adsorption effect, fixing a triple-helix molecular switch THMS on the electrode by virtue of a gold-sulfur bond and a palladium-sulfur bond, when acrylamide exists, combining acrylamide with an aptamer, forming DNAzyme by virtue of single-stranded CDNA1 and single-stranded CDNA2, and driving a DNA walker amplification strategy in the presence of Mg < 2 + >, so that a hairpin HP1 is sheared, and a large amount of single-stranded S1 is obtained. According to the method, S1, signal tags combined on an electrode are reduced and signals are reduced by destroying a triple helix structure, so that acrylamide detection is realized. The electrochemical biosensor constructed by the method disclosed by the invention has relatively high specificity and relatively high sensitivity.

This invention discloses a molecularly switched adsorbent for adsorbing triphenylmethane dyes and its application, belonging to the field of wastewater treatment technology. The molecularly switched adsorbent proposed in this invention can rapidly remove triphenylmethane dyes such as crystal violet, malachite green, and methylene blue dyes from water; it can remove tens of ppm concentrations of crystal violet, malachite green, and methylene blue dyes from water in a very short time (90 min), with a removal rate of ≥92%. Moreover, this adsorbent can maintain a removal rate of ≥90% under a wide pH range (6-11) and high ion concentration conditions (1M). The activation and regeneration conditions of the adsorbent of this invention are mild; dye molecules can be removed by elution in a low-concentration alkaline solution only a few times. The adsorbent can be continuously recycled at least 10 times without significant attenuation of adsorption capacity.

The present invention relates to a capsule luminescence pigment comprising a luminescent substance and a photochemically switchable absorber as a molecular switch, wherein the luminescent substance and the photochemically switchable absorber are dissolved and / or dispersed as separate molecules in a polymer particle, the luminescent substance has an energy transfer level relevant for luminescence, the photochemically switchable absorber has a first absorption spectrum in a first switching state and a second absorption spectrum in a second switching state, wherein the energy transfer level relevant for the luminescence of the luminescent substance falls significantly into an absorption band of the photochemically switchable absorber in the second switching state, so that significantly more intense luminescence of the luminescent substance occurs in the first switching state than in the second switching state of the photochemically switchable absorber.The present invention further relates to a switchable luminescent printing ink comprising a capsule luminescent pigment as described herein and to a security document, preferably a banknote, comprising an imprint with a first switchable luminescent printing ink as described herein.

The application discloses a spiropyran derivative with color and fluorescence dual switch, a photochromic material and a preparation method and application thereof, the spiropyran derivative is a compound with a chemical structure general formula (1); and further discloses a photochromic material prepared by mixing the spiropyran derivative with an epoxy resin and a preparation method thereof. The aromatic fluorescent group is introduced into the side group of the spiropyran material, the conjugated system of the fluorescent group itself is large, and thus the fluorescence emission intensity after the spiropyran is opened is increased. The compound shows good photochromic and photoluminescent effects in organic solvents and solid media; the fluorescent group and the spiropyran material are combined, and a molecular switch material with significant color and fluorescence changes can be prepared; the spiropyran derivative can be used for preparing a two-dimensional optical display device and an intelligent material molecular switch.

This invention discloses a quinoline hydrazone ligand, its Fe(II) spin-crossover complex, and a method for synthesis. The quinoline hydrazone ligand is ( E )-4-bromo- N' -(quinoline-8-methylene)benzoylhydrazine, with the structural formula shown, and the complex structure shown. The ligands of this invention have sufficiently strong coordination ability with metals. The complex is not easily oxidized in air, does not easily dissociate in solution, and has good acid resistance. The complex is in a low-spin state and is suitable for photoacid switching in solution, making it applicable to fields such as spin-crossed molecular switches and acid-base stimulation sensors.

The invention belongs to the field of new materials, and particularly relates to a benzoxazine phenoxazine modified dithienyl ethylene derivative as well as a preparation method and application of the benzoxazine phenoxazine modified dithienyl ethylene derivative. The invention provides a full visible light regulated benzoxazine phenoxazine conjugate modified dithienyl ethylene molecular switch, the photochromic degree of the molecular switch is the same as the effect under ultraviolet irradiation when the molecular switch drives a ring-closing reaction through visible light, and the molecular switch has high visible light responsiveness and light isomerization conversion rate.

The present invention relates to a capsule luminescence pigment comprising a luminescent substance and a photochemically switchable absorber as a molecular switch, wherein the luminescent substance and the photochemically switchable absorber are dissolved and / or dispersed as separate molecules in a polymer particle, the luminescent substance has an excitation spectrum, the photochemically switchable absorber has a first absorption spectrum in a first switching state and a second absorption spectrum in a second switching state, the first absorption spectrum of the photochemically switchable absorber does not significantly overlap with the excitation spectrum of the luminescent substance, and the second absorption spectrum of the photochemically switchable absorber significantly overlaps with the excitation spectrum of the luminescent substance, so that in the first switching state significantly more intense luminescence of the luminescent substance occurs than in the second switching state of the photochemically switchable absorber.The present invention further relates to a switchable luminescent printing ink comprising a capsule luminescent pigment as described herein and to a security document, preferably a banknote, comprising an imprint with a first switchable luminescent printing ink as described herein.

The application provides a DNA enzyme triple helix molecular switch detection system suitable for a personal blood glucose meter, and a preparation method and application thereof, and belongs to the technical field of biosensors.The DNA enzyme triple helix molecular switch comprises an aptamer containing two nucleotide arm segments and DNA enzyme covalently coupled with a magnetic bead; the two nucleotide arm segments of the aptamer form a triple helix structure with the DNA enzyme; and the two nucleotide arm segments comprise a 5' end nucleotide sequence SEQ ID NO.1 of the aptamer and a 3' end nucleotide sequence SEQ ID NO.2 of the aptamer.The application uses a terminal fixation strategy to limit the flexibility of the original aptamer, stabilize the spatial conformation thereof, improve the affinity of the aptamer to the target, utilize the triple helix molecular switch containing the DNA enzyme and the nano-enzyme-gold nanoparticles, and realize accurate and sensitive detection of trace pollutants in food through determination of the reduction range of the glucose concentration in the detection liquid by using the household blood glucose meter.

The application discloses a kind of multiple stimulus response type Zn (II) -DASA complex molecular switch and preparation method and application thereof.The Zn (II) -DASA complex molecular switch is prepared by grafting DASA ligand with salicylaldehyde acylhydrazone and ZnCl2 in methanol, and crystal is obtained by solution evaporation method.The Zn (II) -DASA complex molecular switch of the application exhibits multifunctional switching characteristics of solid-state light, heat and water-induced color change and fluorescence emission, and has wide application prospect in the field of information storage medium such as photo-thermal color-reproducible erasable material, intelligent display material or anti-fake encryption material.

The invention discloses an EVOH packaging material with long-acting antibacterial and antioxidant functions and a preparation method of the EVOH packaging material, wherein the EVOH packaging material is obtained on the basis of a dynamic molecular switch and a microcapsule targeted release technology. The material comprises an EVOH matrix (A), an environmental response type molecular switch polymer (B) and an intelligent microcapsule (C) sensitive to at least one putrefying biomarker, and a compatibilizer (D). In a normal state, the molecular switch is turned off, and the release of the active agent is inhibited; when the environment humidity is increased or the concentration of the putrefying biomarker is changed, the molecular switch is turned on, and the microcapsule is disintegrated in a targeted manner, so that the efficient and intelligent release of active substances is realized. The material can prolong the effective period of an active agent by more than 300%, realizes accurate intervention on food spoilage, and is suitable for the field of high-end intelligent packaging.

The application discloses a HAT probe with adjustable switch characteristics and application thereof, which comprises a secondary structure capable of forming specific steric hindrance, the secondary structure is a convex "bubble" or "ring", and the core of the secondary structure is a polydeoxythymidine sequence with a length of 5-30 nucleotides. The HAT probe has a "switch" regulation function: a molecular switch is constructed by utilizing the inhibition and activation of Cas12a activity of the complete structure and the linear structure after cutting of the HAT probe. The "switch" can respond to specific biomolecules (such as miRNA, protein), and convert the existence of the biomolecules into a detectable signal.

The invention relates to the technical field of high-value utilization of marine biological resources, and discloses a high-activity marine biological peptide prepared by an enzymolysis method and application of the high-activity marine biological peptide in food. The target high-activity marine biological peptide and an affinity molecular switch sensitive to the concentration change of the target high-activity marine biological peptide are jointly fixed on a magnetic nano-carrier with bitter peptide adsorption functional groups on the surface to obtain the intelligent enzyme carrier; and adding the intelligent enzyme carrier into a reaction system containing a marine protein raw material and a first amphiphilic microcapsule wall material to carry out an enzymatic hydrolysis reaction in the reaction process. By constructing an intelligent enzyme carrier integrated with a directional enzyme and an affinity molecular switch, precise cutting and process self-control of an enzymolysis reaction are realized, and the affinity molecular switch can respond to the change of the concentration of the high-activity marine biological peptide and spontaneously adjust the enzyme activity, so that excessive degradation of a target product is effectively avoided.

The application discloses a reversible single-molecule switch based on local cation regulation and belongs to the technical field of molecular electronics. The application is based on the crack junction technology of an electrochemical scanning tunneling microscope. The distribution of local cations in an interface double electric layer is changed by controlling an electric potential, so that the state of a carboxylic acid molecule is influenced. The contact action of the carboxylic acid molecule and a gold needle tip is adjusted, and a single-molecule switch is realized. The electric potential is-0.5-0V. The application proves the influence of local cations in an electrochemical interface on carboxyl molecules, and opens up a new way for realizing the practical application of a reversible single-molecule switch through a gate electrode.

The invention discloses internal peroxide singlet oxygen carrier molecules capable of being quickly converted and application thereof, and belongs to the field of functional molecule design and intelligent materials. The molecules are functionalized on the ferroferric oxide magnetic nano material, and a singlet oxygen storage system which is kept inert under the normal temperature condition is successfully constructed. When the system is in contact with fluorine ion sources such as tetrabutylammonium fluoride and the like, carbon-silicon bonds are triggered to break through nucleophilic substitution reaction, so that a molecular structure is promoted to be quickly converted into 1, 4-dimethylnaphthalene endoperoxide and is subjected to ring cracking, singlet oxygen is released, and accurate regulation and control of a molecular switch are realized. A DPBF probe verifies that singlet oxygen release of the system can be controlled through fluorine ions, and excellent time-space controllability is shown. Furthermore, a magnetically-controlled singlet oxygen source is constructed by loading on a ferroferric oxide nano-carrier, and a singlet oxygen nano-carrier controlled by a magnetic field is constructed; the limitation that a traditional photosensitizer depends on an external light source and oxygen is broken through, and wide application prospects are achieved.

The application discloses a ClHAK8 gene promoter variation site related to watermelon salt tolerance and application thereof, and relates to the technical field of biology.The ClHAK8 gene promoter variation site is located at the position of-1534 to-1529 bp in the ClHAK8 gene promoter region; and the nucleotide sequence of the ClHAK8 gene is shown as SEQ ID NO.1.It is first clarified in the application that the presence or absence of the W-box element (TTGACC) at the position of-1534 to-1529 bp in the upstream promoter region of the watermelon ClHAK8 gene is a core molecular switch for regulating watermelon salt tolerance.The application clarifies the molecular mechanism of the variation of the promoter cis-element for affecting the salt stress response of watermelon by regulating the expression of a potassium transport gene, perfects the salt tolerance regulation network of watermelon, and provides a stable and practical detection tool for the molecular marker assisted breeding of salt-tolerant watermelon.

The invention belongs to the technical field of monomolecular organic electronics, and discloses a preparation method, performance research and application of imidazole-based organic molecules. On the basis of imidazole organic molecules, methylthio is used as an anchoring group, and the imidazole organic molecules have the following structure: # imgabs0 #. The methylthio anchoring groups are introduced into the unique conjugated structure and two ends of the imidazole molecules, and under certain conditions, the molecules change from a single molecular system to a bimolecular system, so that the molecular weight is increased, and the molecular weight is increased. And the conductivity value, the molecular pulling length and other corresponding characteristics can also change correspondingly. Due to the fact that free ions in imidazole molecules can react with adjacent molecules to a certain extent under specific conditions, the imidazole molecules can serve as molecular devices related to molecular switches and the like to be applied in monomolecular devices, and the imidazole molecules can also be used for information storage and encryption due to the performance of response under the specific conditions. Therefore, the molecule has a good application prospect in the field of organic single-molecule wires.

The present invention discloses a multicolor fluorescent molecular switch, which has a merocyanine structure and a pyrene group, and its structural formula is as shown in I. The molecular switch provided by the present invention can adjust its fluorescence switching performance by changing the merocyanine structure and the aggregation state of the molecule, and can realize the switching of multiple fluorescent colors. It can have the advantages of visible light stimulus response, high isomerization efficiency, spontaneous recovery, etc. It can be converted by more than 95% within 120 seconds under white light stimulation, can be switched cyclically for more than 5 times, and has good fatigue resistance. Moreover, the preparation method is simple. The present invention provides a synthetic idea of a fluorescent molecular switch with high conversion rate and simple adjustment of fluorescence performance, which greatly enriches the spiropyran-based molecular switch system.

The present invention relates to the field of lysosome imaging probes and optical super-resolution imaging. A light-controlled fluorescent molecular switch targeting lysosomes has a structural formula as shown in Formula I: wherein R is any one of NMe2 and NEt2.

The invention relates to the technical field of molecular switches, and discloses an optical switch molecule and a preparation method and application thereof. The structural general formula of the optical switch molecule is as follows: R1 is a hydrogen bond acceptor group, R2 is an electron donating group or an electron withdrawing group, and R3 and R4 are spatial substituent groups. According to the invention, sp3 hybrid carbon atoms are used as bridging units, and a series of space folding type ESIPT optical switch molecules are constructed for the first time and are applied to the aspects of chemical sensing, information encryption, data storage and the like. According to the method, light is used as an external stimulation condition to induce the photoswitch molecules to generate a rapid six-level photophysical-chemical process and generate a cis-trans isomerization process in an excited state, so that the photoswitch molecules are macroscopically represented as high-contrast color change before and after photostimulation.

The present invention relates to automotive products, as well as uses thereof, which can facilitate warming of engine oil in an energy efficient and, therefore, more environmentally friendly manner. More specifically, a fluid reservoir is disclosed that includes a double walled vessel that defines a fluid containing compartment and has a self-contained region that shares a wall with the fluid containing compartment. A composition in the self-contained region at least partially covers the shared wall surface, and one or more light sources are positioned in the self-contained region to induce a phase change in the composition. The composition includes a phase-change material (PCM) and a molecular switch material. This fluid reservoir can be used to heat oil in an engine or oil pan.

The invention belongs to the technical field of gene and agricultural product storage, and discloses a PRX3 gene and application of acetylation modification of the PRX3 gene in regulation and control of dormancy and / or oxidation, the PRX3 gene is StPRX3, and the gene sequence of the PRX3 gene is shown as SEQ ID NO.1. The positive regulation effect of the deacetylation protease (StPRX3) on the dormancy of the potatoes in the storage period is confirmed for the first time by integrating bioinformatics prediction and an instantaneous conversion technology. A technical path for accurately regulating and controlling the dormancy process through a molecular means is also established, the uncertainty influence of environmental factors on the tuber quality in a traditional storage mode is effectively reduced, a key molecular switch for connecting apparent modification and redox regulation and control is determined, and a new thought is provided for preservation of the harvested potatoes.

Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

The invention discloses a gene chip biosensor based on DNAzyme and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost of a single nucleotide polymorphism detection method in the prior art, the invention provides a gene chip biosensor based on DNAzyme, and the gene chip biosensor comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The invention provides a protease-free and space-oriented biosensing strategy, accurate SNP recognition is realized by using a molecular switch based on a DNAzyme probe, and signal amplification is enhanced and background noise is inhibited by using a steric hindrance parallel amplification system, so that the detection performance is remarkably improved; the gene chip biosensor provided by the invention can be applied to genetic typing and character selection in crop improvement and other applications.

The invention relates to the technical field of liquid crystal materials, and discloses a preparation method and application of a liquid crystal membrane capable of dynamically programming and locking a molecular switch, and the method comprises the following steps: blending a liquid crystal polymerizable monomer, a polymerizable monomer containing a dynamic covalent bond, a polymerizable molecular switch, dithiol, polythiol, a photoinitiator, a thermal initiator and toluene; and carrying out click reaction in a dark environment, and carrying out self-polymerization reaction under the irradiation of green light to obtain the liquid crystal film. According to the invention, a dynamic cross-linked polymer network formed by dynamic covalent bonds endows the molecular switch in the system with an adjustable molecular motion environment, and the motion environment is freely or limitedly switched according to the size of the free volume. Therefore, the molecular switch can endow the liquid crystal polymer with programmable stimulation response capability, and programming locking or unlocking can be realized by adjusting the free volume. The invention provides a new perspective for improving the controllability of the stimulation response behavior of the intelligent material, and has huge potential in the fields of anti-counterfeiting, information encryption and the like.

This invention belongs to the field of photonics, functional optical materials and devices, specifically relating to a spectral dynamic programming device, its fabrication method, and its applications. The spectral dynamic programming device has a three-layer structure, comprising, from bottom to top, a light-emitting thin film, a cholesteric liquid crystal layer, and a circular polarization analyzer. The light-emitting thin film is a fluorescent thin film or an organic room-temperature phosphorescent polymer thin film; the cholesteric liquid crystal layer is a mixture of a photoresponsive chiral molecular switch, a static inverse chiral dopant, and a nematic liquid crystal host. By alternately irradiating the liquid crystal layer with light of different wavelengths, the helical pitch of the liquid crystal layer can be reversibly and continuously adjusted, allowing its photonic bandgap reflection band to sweep across a wide spectral range. This dynamically "tailors" the emission spectrum of the lower light-emitting thin film, achieving continuous and reversible programming of the output color. This device can also generate emission with a high circular polarization asymmetry factor and possesses excellent cycling stability, making it applicable to the optical field.