37 results about "Molecular switch" patented technology

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.

The invention provides a DNA enzyme triple-helix molecular switch detection system adaptive to a personal glucometer as well as a preparation method and application of the DNA enzyme triple-helix molecular switch detection system, and belongs to the technical field of biosensors. The DNA enzyme triple-helix molecular switch disclosed by the invention comprises an aptamer containing two nucleotide arm segments and DNA enzyme covalently coupled with a magnetic bead, two nucleotide arm segments of the aptamer and DNA enzyme form a triple helix structure; the two nucleotide arm segments comprise a nucleotide sequence SEQ ID NO.1 at the 5'end of the aptamer and a nucleotide sequence SEQ ID NO.2 at the 3 'end of the aptamer. According to the invention, the flexibility of an original aptamer is limited by using a terminal fixation strategy, the spatial conformation of the original aptamer is stabilized, the affinity of the aptamer to a target is improved, and the sensitivity of the aptamer to the target is improved by using a triple-helix molecular switch containing DNA enzyme and nano-enzyme-gold nanoparticles. Accurate and sensitive detection of trace pollutants in food is realized by measuring the reduction amplitude of the glucose concentration in the detection liquid through a household glucometer.

The application provides a DNA enzyme triple helix molecular switch detection system suitable for a personal blood glucose meter, and a preparation method and application thereof, and belongs to the technical field of biosensors.The DNA enzyme triple helix molecular switch comprises an aptamer containing two nucleotide arm segments and DNA enzyme covalently coupled with a magnetic bead; the two nucleotide arm segments of the aptamer form a triple helix structure with the DNA enzyme; and the two nucleotide arm segments comprise a 5' end nucleotide sequence SEQ ID NO.1 of the aptamer and a 3' end nucleotide sequence SEQ ID NO.2 of the aptamer.The application uses a terminal fixation strategy to limit the flexibility of the original aptamer, stabilize the spatial conformation thereof, improve the affinity of the aptamer to the target, utilize the triple helix molecular switch containing the DNA enzyme and the nano-enzyme-gold nanoparticles, and realize accurate and sensitive detection of trace pollutants in food through determination of the reduction range of the glucose concentration in the detection liquid by using the household blood glucose meter.

The invention discloses an EVOH packaging material with long-acting antibacterial and antioxidant functions and a preparation method of the EVOH packaging material, wherein the EVOH packaging material is obtained on the basis of a dynamic molecular switch and a microcapsule targeted release technology. The material comprises an EVOH matrix (A), an environmental response type molecular switch polymer (B) and an intelligent microcapsule (C) sensitive to at least one putrefying biomarker, and a compatibilizer (D). In a normal state, the molecular switch is turned off, and the release of the active agent is inhibited; when the environment humidity is increased or the concentration of the putrefying biomarker is changed, the molecular switch is turned on, and the microcapsule is disintegrated in a targeted manner, so that the efficient and intelligent release of active substances is realized. The material can prolong the effective period of an active agent by more than 300%, realizes accurate intervention on food spoilage, and is suitable for the field of high-end intelligent packaging.

The application discloses a HAT probe with adjustable switch characteristics and application thereof, which comprises a secondary structure capable of forming specific steric hindrance, the secondary structure is a convex "bubble" or "ring", and the core of the secondary structure is a polydeoxythymidine sequence with a length of 5-30 nucleotides. The HAT probe has a "switch" regulation function: a molecular switch is constructed by utilizing the inhibition and activation of Cas12a activity of the complete structure and the linear structure after cutting of the HAT probe. The "switch" can respond to specific biomolecules (such as miRNA, protein), and convert the existence of the biomolecules into a detectable signal.

The invention relates to the technical field of high-value utilization of marine biological resources, and discloses a high-activity marine biological peptide prepared by an enzymolysis method and application of the high-activity marine biological peptide in food. The target high-activity marine biological peptide and an affinity molecular switch sensitive to the concentration change of the target high-activity marine biological peptide are jointly fixed on a magnetic nano-carrier with bitter peptide adsorption functional groups on the surface to obtain the intelligent enzyme carrier; and adding the intelligent enzyme carrier into a reaction system containing a marine protein raw material and a first amphiphilic microcapsule wall material to carry out an enzymatic hydrolysis reaction in the reaction process. By constructing an intelligent enzyme carrier integrated with a directional enzyme and an affinity molecular switch, precise cutting and process self-control of an enzymolysis reaction are realized, and the affinity molecular switch can respond to the change of the concentration of the high-activity marine biological peptide and spontaneously adjust the enzyme activity, so that excessive degradation of a target product is effectively avoided.

The application discloses a reversible single-molecule switch based on local cation regulation and belongs to the technical field of molecular electronics. The application is based on the crack junction technology of an electrochemical scanning tunneling microscope. The distribution of local cations in an interface double electric layer is changed by controlling an electric potential, so that the state of a carboxylic acid molecule is influenced. The contact action of the carboxylic acid molecule and a gold needle tip is adjusted, and a single-molecule switch is realized. The electric potential is-0.5-0V. The application proves the influence of local cations in an electrochemical interface on carboxyl molecules, and opens up a new way for realizing the practical application of a reversible single-molecule switch through a gate electrode.

The application discloses a ClHAK8 gene promoter variation site related to watermelon salt tolerance and application thereof, and relates to the technical field of biology.The ClHAK8 gene promoter variation site is located at the position of-1534 to-1529 bp in the ClHAK8 gene promoter region; and the nucleotide sequence of the ClHAK8 gene is shown as SEQ ID NO.1.It is first clarified in the application that the presence or absence of the W-box element (TTGACC) at the position of-1534 to-1529 bp in the upstream promoter region of the watermelon ClHAK8 gene is a core molecular switch for regulating watermelon salt tolerance.The application clarifies the molecular mechanism of the variation of the promoter cis-element for affecting the salt stress response of watermelon by regulating the expression of a potassium transport gene, perfects the salt tolerance regulation network of watermelon, and provides a stable and practical detection tool for the molecular marker assisted breeding of salt-tolerant watermelon.

The invention relates to the technical field of molecular switches, and discloses an optical switch molecule and a preparation method and application thereof. The structural general formula of the optical switch molecule is as follows: R1 is a hydrogen bond acceptor group, R2 is an electron donating group or an electron withdrawing group, and R3 and R4 are spatial substituent groups. According to the invention, sp3 hybrid carbon atoms are used as bridging units, and a series of space folding type ESIPT optical switch molecules are constructed for the first time and are applied to the aspects of chemical sensing, information encryption, data storage and the like. According to the method, light is used as an external stimulation condition to induce the photoswitch molecules to generate a rapid six-level photophysical-chemical process and generate a cis-trans isomerization process in an excited state, so that the photoswitch molecules are macroscopically represented as high-contrast color change before and after photostimulation.

The present invention relates to automotive products, as well as uses thereof, which can facilitate warming of engine oil in an energy efficient and, therefore, more environmentally friendly manner. More specifically, a fluid reservoir is disclosed that includes a double walled vessel that defines a fluid containing compartment and has a self-contained region that shares a wall with the fluid containing compartment. A composition in the self-contained region at least partially covers the shared wall surface, and one or more light sources are positioned in the self-contained region to induce a phase change in the composition. The composition includes a phase-change material (PCM) and a molecular switch material. This fluid reservoir can be used to heat oil in an engine or oil pan.

The invention belongs to the technical field of gene and agricultural product storage, and discloses a PRX3 gene and application of acetylation modification of the PRX3 gene in regulation and control of dormancy and / or oxidation, the PRX3 gene is StPRX3, and the gene sequence of the PRX3 gene is shown as SEQ ID NO.1. The positive regulation effect of the deacetylation protease (StPRX3) on the dormancy of the potatoes in the storage period is confirmed for the first time by integrating bioinformatics prediction and an instantaneous conversion technology. A technical path for accurately regulating and controlling the dormancy process through a molecular means is also established, the uncertainty influence of environmental factors on the tuber quality in a traditional storage mode is effectively reduced, a key molecular switch for connecting apparent modification and redox regulation and control is determined, and a new thought is provided for preservation of the harvested potatoes.

Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

This invention belongs to the field of photonics, functional optical materials and devices, specifically relating to a spectral dynamic programming device, its fabrication method, and its applications. The spectral dynamic programming device has a three-layer structure, comprising, from bottom to top, a light-emitting thin film, a cholesteric liquid crystal layer, and a circular polarization analyzer. The light-emitting thin film is a fluorescent thin film or an organic room-temperature phosphorescent polymer thin film; the cholesteric liquid crystal layer is a mixture of a photoresponsive chiral molecular switch, a static inverse chiral dopant, and a nematic liquid crystal host. By alternately irradiating the liquid crystal layer with light of different wavelengths, the helical pitch of the liquid crystal layer can be reversibly and continuously adjusted, allowing its photonic bandgap reflection band to sweep across a wide spectral range. This dynamically "tailors" the emission spectrum of the lower light-emitting thin film, achieving continuous and reversible programming of the output color. This device can also generate emission with a high circular polarization asymmetry factor and possesses excellent cycling stability, making it applicable to the optical field.

This invention discloses a system, method, and reagent kit for detecting biochemical oxygen demand (BOD) in wastewater, belonging to the field of environmental monitoring technology. The detection system of this invention includes at least two independent detection channels and a signal processing module. Each detection channel independently includes an enzymatic conversion module, which contains hydrolytic enzyme systems, oxidoreductase systems, and coenzyme NAD for specific types of organic matter. + The signal transduction and amplification module includes a CRISPR-Cas effector protein, a fluorescent reporter probe, and a molecular switch. The signal processing module detects the fluorescence signal intensity of each detection channel and converts the fluorescence signal into the biochemical oxygen demand (BOD) contribution value of each detection channel based on a pre-stored standard curve, summing them to obtain the total BOD. This invention effectively overcomes the detection uncertainty caused by fluctuations in microbial activity and differences in substrate degradation rates in traditional methods, significantly shortening the detection cycle to within 30 minutes.

The application provides a method for building a 9-bit parity check molecular switch circuit, and the steps are as follows: based on DNA strand displacement technology, the structure and reaction mechanism of the molecular switch circuit are described; the reaction rate and leakage rate are set, and the input signal of the molecular switch circuit and the concentration ratio of the molecular switch at each stage are optimized; the fan-in molecular switch circuit and the fan-out molecular switch circuit are set according to the molecular switch circuit; the 3-bit parity check molecular switch circuit is built based on the fan-in molecular switch circuit and the fan-out molecular switch circuit by using the switch canvas routing strategy, the 9-bit parity check molecular switch circuit is built by using three 3-bit parity check molecular switch circuits with the same structure; and the correctness of the 9-bit parity check molecular switch circuit is verified by using a Simbiology simulation platform. In the construction of a large-scale complex logic circuit, the application has a simple and unified DNA chain structure, a fast reaction speed and good stability, and provides a new scheme for the construction of a modular DNA circuit.

The application discloses a preparation method of a stimulus-responsive anti-pollution easy-to-clean nanofiltration membrane and belongs to the technical field of nanofiltration membranes. In the application, an amino monomer containing a dynamic disulfide bond (S-S) is used as a molecular switch, the disulfide bond can be reversibly broken into a mercapto group (-SH) under the action of a reducing agent and re-oxidized into a disulfide bond under the action of an oxidizing agent, the disulfide bond is introduced into a polyamide separation layer through an interfacial polymerization technology, and a membrane pore dynamic regulation mechanism of "reduction expansion-oxidation shrinkage" is constructed. When the membrane is polluted, the membrane pores are expanded through reduction treatment, and the pollutants are physically detached; then, the membrane pore structure is restored through oxidation treatment, and self-cleaning is realized.

The application is a divisional application of the Chinese invention patent application with application number 202410914209.2, application date of 2024.07.09 and invention name of "Molecular switch for controlling the transformation of colorectal cancer cell genome from MSS to MSI, inhibitor and application thereof". The application discloses the application of verteporfin or VT103 in reducing the level of MMR protein, and belongs to the technical field of medicine. The application finds that verteporfin and VT103 can transform the microsatellite stable state of colorectal cancer cell genome into a microsatellite unstable state, induce the tumor cells with microsatellite instability to exhibit reduced expression of mismatch repair proteins, increased mutation load and increased neoantigen load, can increase the mutation frequency of tumor cells, stimulate the generation of tumor neoantigen peptides, realize the transformation from "cold" tumor cells that cannot respond to immunotherapy to "hot" tumor cells, and provide a theoretical basis for the development of drugs for microsatellite stable digestive tract tumors and the treatment strategy of microsatellite stable tumors by targeting YAP / TAZ-TEAD combined with immunotherapy.

The invention discloses a supramolecular liquid crystal compound and a preparation method thereof. The liquid crystal property and the light response property of the compound are mainly tested by POM (Polyoxymethylene), UV (Ultraviolet) and other methods. The compound can form a smectic phase (SmA), the photoresponse property of the compound is researched through an ultraviolet spectrum, and the result shows that the compound has the stratified phase (SmA), has good photoresponse behavior and reversible photophysical properties, and is expected to be used for photoelectric molecular switches.

This invention discloses a dibenzylamine-bridged DASA molecular switch for salicylaldehyde hydrazone and its preparation method. The DASA molecule has the structure 4-((benzyl((1E,3Z)-5-(1,3-dimethyl-2,4,6-trioxotetrahydropyrimidine-5(2H)-ylidene)-4-hydroxypentan-1,3-dien-1-yl)amino)methyl)-N′-((E)-2-hydroxybenzylene)benzoylhydrazide, which is prepared by reacting the donor (E)-4-((benzylamino)methyl)-N′-(2-hydroxybenzylene)benzoylhydrazide with the acceptor 5-(furan-2-methylene)-1,3-dimethylpyrimidine-2,4,6(1H,3H,5H)-trione in methanol. Its advantages include: exhibiting excellent negative photochromic behavior in moderately strong aprotic polar solvents such as dichloromethane and acetonitrile; undergoing reversible linear-ring isomerization under visible light irradiation; showing significant changes in absorption peaks; and exhibiting good cyclic fatigue resistance. Furthermore, the introduced salicylaldehyde hydrazone group enhances its responsiveness to acids, bases, and metal ions, enabling reversible switching in various protic and aprotic solvents and selective recognition of different metal ions, thus showing broad application prospects in colorimetric sensing and metal ion detection.

The invention relates to an acid-resistant nanofiltration membrane and a preparation method thereof, the acid-resistant nanofiltration membrane comprises a porous support layer and an active separation layer formed on the surface of the porous support layer; the active separation layer is a polyamide network layer formed by carrying out interfacial polymerization reaction on a water-phase solution containing a phosphoric acid esterification piperazine derivative and an oil-phase solution containing trimesoyl chloride on the surface of the porous support layer; the phosphate esterification piperazine derivative is a compound in which a phosphate ester group is covalently connected to a nitrogen atom of a piperazine ring through a connecting arm, and the polyamide network layer has reversible hydrogen bond interaction sites introduced by the phosphate esterification piperazine derivative. The membrane has the beneficial effects that a molecular switch which is dynamically adjusted along with the pH is constructed in the membrane, so that the separation performance of the membrane can realize intelligent response along with the change of the environmental pH.

This invention belongs to the field of new materials technology, and more specifically, relates to an alkoxytriphenylamine conjugated modified dithienylethylene derivative and its applications. The dithienylethylene derivative has structural units as shown in formula (I): wherein R1O and R2O are each independently a C1-C4 alkoxy group, and Ar is a C6-C4 alkoxy group. 14 The aryl group. The alkoxytriphenylamine-modified dithienylethylene molecular switch designed in this invention has an absorption wavelength red-shifted to the visible light region, so that the ring-closing reaction can be driven by visible light, while the ring-opening reaction itself is driven by long-wavelength visible light. This achieves full visible light control of the dithienylethylene molecular switch and has strong and efficient visible light responsiveness and remarkable photoisomerization conversion rate.

The invention provides a small molecule switch induced AAV8-tetracycline switch-hEPO (human erythropoietin) for treating anemia. Specifically, the invention provides a construction for inducing expression of a tetracycline regulatory sequence and a target gene (such as human erythropoietin (hEPO)), a vector containing the construction, a host cell, a gene delivery system, a pharmaceutical composition, a product combination and application of the construction in treatment of diseases (such as anemia). According to the invention, a wide application prospect is provided for accurate, controllable, changeable and customizable treatment, and the safety of gene treatment is improved. For example, under regulation and control of a tetracycline switch, the anemia treatment method provided by the invention has very high safety while effectively treating anemia, and the problems of serious and fatal polycythemia and the like caused by continuous increase of erythrocyte generation due to excessive production of EPO are avoided.

The application discloses a Fe(II) spin crossover material based on perchlorate, and a preparation method and application thereof. The application adopts a room temperature volatilization method to prepare [Fe(bamp)2]·(ClO4)2 with a higher yield, and compared with a high-temperature and high-pressure solvothermal synthesis method and a poor solvent solvent diffusion method, the method has the advantages of simple reaction condition and easy realization. The organic ligand 2,6-bis(amino methyl) pyridine has rich benzene groups and amino groups, and is easy to construct a pi-pi stacking and hydrogen bonding to finely control spin transition properties; meanwhile, ClO4 ‑ The control enhances the pi-pi intermolecular stacking effect, and under the synergistic action of the two, the material appears spin transition behavior. The Fe(II) spin crossover magnetic material has the characteristics of cheap raw materials, stable structure and performance, and the synthesized spin crossover material can be applied to the fields of molecular switch, molecular sensor and ultra-high density information storage.

Provided herein is a system, apparatus and method for generating a three-dimensional image and / or printing a three dimensional structures, the system comprising: a medium comprising a photocurable monomer and an optical molecular switch molecule that has a non-fluorescent state and a fluorescent state, wherein the monomer forms a polymer upon exposure to the emitted light. Also taught are novel fluorescent photoswitch molecules.

This invention discloses a molecular switch-type nucleic acid framework fluorescent probe and its application in the detection of ATP molecules, a biomarker for depression, belonging to the field of biodetection technology. The fluorescent probe of this invention has a tetrahedral structure, comprising three complementary DNA single strands and four additional DNA single strands forming the tetrahedral structure. The three complementary DNA single strands include a Cy3 dye modified with a central backbone, a phosphate group modified at the 5' end, and a Cy5 dye modified at the 3' end. The fluorescent probe of this invention is activated by ATP, rapidly responding to ATP to emit a fluorescent signal, and is deactivated by an endonuclease, automatically returning to its initial state. This probe enables dynamic cyclic detection of ATP molecules and can directly perform rapid, highly sensitive, and highly selective quantitative analysis of the purine neurotransmitter ATP in complex biological samples such as plasma and urine, providing an effective means for early screening of neurological diseases.

The invention discloses a preparation method of a molecular switch type UHPC (Ultra High Performance Concrete) internal curing agent. The preparation method comprises the following steps: preparing end group sulfhydrylated cyclodextrin; preparing an end group sulfhydrylation hyperfine network structure; and carrying out oxidation reaction on the product in an aqueous solution to synthesize the molecular switch intelligent UHPC internal curing agent. According to the internal curing agent, a-S-S-dynamic bond is introduced to serve as a molecular switch, response and accurate water release can be achieved in the UHPC hydration high-temperature stage, and the problems of interface weakening and later water shortage caused by premature water release of a traditional internal curing agent are effectively solved. The prepared internal curing agent has excellent water absorption and storage capacity and temperature-triggered water release characteristics, can remarkably reduce drying shrinkage of UHPC, has no adverse effect on compressive strength of the UHPC, and is suitable for internal curing engineering of ultra-high performance concrete.

Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

The invention discloses a method for screening tumor-related drug targets aiming at fibroblasts. According to the invention, the tumor-related drug targets in the fibroblasts are screened by implementing double-disturbance scCRISPR. According to the invention, not only can a causal gene regulation network of myofibroblast differentiation be systematically analyzed in a single experiment, but also key molecular switches such as ADAM12 capable of reprogramming myofibroblasts from a cancer promoting state to an anti-cancer state are successfully identified. The method can provide a brand new research normal form and a definite therapeutic target for solving the problem of drug resistance of myofibroblast mediated immunotherapy.

The invention discloses a near-infrared emission asymmetric BODIPY fluorescent material and a preparation method of the near-infrared emission asymmetric BODIPY fluorescent material. Diketopyrrolopyrrole with an asymmetric structure is used as a raw material and is subjected to a condensation reaction with an aromatic acetonitrile compound and a coordination reaction with BF3. Et2O to prepare the near-infrared emission asymmetric BODIPY fluorescent molecule with an electronic push-pull effect and an asymmetric structure. The method has the characteristics of simple synthesis steps, mild conditions, easiness in structural modification and function regulation and control, high molar extinction coefficient, large Stokes displacement and the like, and has potential application prospects in the fields of fluorescent probes, biological imaging, molecular switches, intelligent sensing and the like.

This invention relates to the field of analytical detection technology, and discloses a high-throughput rapid detection platform for contaminants in food, its construction method, and its applications. The high-throughput rapid detection platform is constructed by immobilizing universally applicable biorecognition sensing probes targeting contaminants on a 96-well plate to achieve high-throughput rapid detection. The biorecognition sensing probes include a Pt NP-modified signal transduction probe (Bond) and an arm-containing aptamer that specifically recognizes the contaminants. This invention constructs an aptamer sensor by modifying Pt NPs onto a triple-helix molecular switch signal transduction probe. Simultaneously, utilizing its peroxidase mimic activity and fluorescence quenching capability, the activity of Pt NPs is modulated via the triple-helix molecular switch, developing a universal dual-mode aptamer sensing platform. This platform retains the inherent advantages of each signal mode while supporting real-time cross-validation, helping to reduce systematic errors and suppress external interference, effectively reducing false positive and false negative rates.