57 results about "Molecular switch" patented technology

The invention discloses a colorimetric aptamer sensor and application thereof, and belongs to the technical field of biological detection. The colorimetric aptamer sensor comprises Cas12a protein, HP, crRNA, dsDNA (double-stranded deoxyribonucleic acid) and a colorimetric probe. The colorimetric biosensor is applied to detection of the ochratoxin A, the content of the ochratoxin A is detected through the colorimetric biosensor, and detection and analysis of a target object can be achieved; cas12a aptamer inhibitors are developed as molecular switches for biosensors, which mediate target recognition through engineering modification of HP hairpin sequences. A new thought for improving the mycotoxin detection sensitivity is explored, and good detection potential is expected to be shown in the field of food safety.

The present invention relates to a modified stem cell and an ex vivo method for preparing defined mature cells from genetic programming of said modified stem cell which can be carried out in absence of certain growth factors, as well as a kit allowing to transform stem cells, and the different uses of the mature cells, for example for in vitro drug screening and toxicology or as cultivated meat. The genetic programming involves a molecular switch between two mutually exclusive programs of stem cell expansion and differentiation. The invention may be used for stem cells or other progenitors cells of any type, from any eukaryotic organism, but finds particular application in pluripotent stem cells from humans and livestock animals.

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.

The invention provides a DNA enzyme triple-helix molecular switch detection system adaptive to a personal glucometer as well as a preparation method and application of the DNA enzyme triple-helix molecular switch detection system, and belongs to the technical field of biosensors. The DNA enzyme triple-helix molecular switch disclosed by the invention comprises an aptamer containing two nucleotide arm segments and DNA enzyme covalently coupled with a magnetic bead, two nucleotide arm segments of the aptamer and DNA enzyme form a triple helix structure; the two nucleotide arm segments comprise a nucleotide sequence SEQ ID NO.1 at the 5'end of the aptamer and a nucleotide sequence SEQ ID NO.2 at the 3 'end of the aptamer. According to the invention, the flexibility of an original aptamer is limited by using a terminal fixation strategy, the spatial conformation of the original aptamer is stabilized, the affinity of the aptamer to a target is improved, and the sensitivity of the aptamer to the target is improved by using a triple-helix molecular switch containing DNA enzyme and nano-enzyme-gold nanoparticles. Accurate and sensitive detection of trace pollutants in food is realized by measuring the reduction amplitude of the glucose concentration in the detection liquid through a household glucometer.

This invention discloses a molecularly switched adsorbent for adsorbing triphenylmethane dyes and its application, belonging to the field of wastewater treatment technology. The molecularly switched adsorbent proposed in this invention can rapidly remove triphenylmethane dyes such as crystal violet, malachite green, and methylene blue dyes from water; it can remove tens of ppm concentrations of crystal violet, malachite green, and methylene blue dyes from water in a very short time (90 min), with a removal rate of ≥92%. Moreover, this adsorbent can maintain a removal rate of ≥90% under a wide pH range (6-11) and high ion concentration conditions (1M). The activation and regeneration conditions of the adsorbent of this invention are mild; dye molecules can be removed by elution in a low-concentration alkaline solution only a few times. The adsorbent can be continuously recycled at least 10 times without significant attenuation of adsorption capacity.

The present invention relates to a capsule luminescence pigment comprising a luminescent substance and a photochemically switchable absorber as a molecular switch, wherein the luminescent substance and the photochemically switchable absorber are dissolved and / or dispersed as separate molecules in a polymer particle, the luminescent substance has an energy transfer level relevant for luminescence, the photochemically switchable absorber has a first absorption spectrum in a first switching state and a second absorption spectrum in a second switching state, wherein the energy transfer level relevant for the luminescence of the luminescent substance falls significantly into an absorption band of the photochemically switchable absorber in the second switching state, so that significantly more intense luminescence of the luminescent substance occurs in the first switching state than in the second switching state of the photochemically switchable absorber.The present invention further relates to a switchable luminescent printing ink comprising a capsule luminescent pigment as described herein and to a security document, preferably a banknote, comprising an imprint with a first switchable luminescent printing ink as described herein.

The application discloses a spiropyran derivative with color and fluorescence dual switch, a photochromic material and a preparation method and application thereof, the spiropyran derivative is a compound with a chemical structure general formula (1); and further discloses a photochromic material prepared by mixing the spiropyran derivative with an epoxy resin and a preparation method thereof. The aromatic fluorescent group is introduced into the side group of the spiropyran material, the conjugated system of the fluorescent group itself is large, and thus the fluorescence emission intensity after the spiropyran is opened is increased. The compound shows good photochromic and photoluminescent effects in organic solvents and solid media; the fluorescent group and the spiropyran material are combined, and a molecular switch material with significant color and fluorescence changes can be prepared; the spiropyran derivative can be used for preparing a two-dimensional optical display device and an intelligent material molecular switch.

This invention discloses a quinoline hydrazone ligand, its Fe(II) spin-crossover complex, and a method for synthesis. The quinoline hydrazone ligand is ( E )-4-bromo- N' -(quinoline-8-methylene)benzoylhydrazine, with the structural formula shown, and the complex structure shown. The ligands of this invention have sufficiently strong coordination ability with metals. The complex is not easily oxidized in air, does not easily dissociate in solution, and has good acid resistance. The complex is in a low-spin state and is suitable for photoacid switching in solution, making it applicable to fields such as spin-crossed molecular switches and acid-base stimulation sensors.

The invention belongs to the field of new materials, and particularly relates to a benzoxazine phenoxazine modified dithienyl ethylene derivative as well as a preparation method and application of the benzoxazine phenoxazine modified dithienyl ethylene derivative. The invention provides a full visible light regulated benzoxazine phenoxazine conjugate modified dithienyl ethylene molecular switch, the photochromic degree of the molecular switch is the same as the effect under ultraviolet irradiation when the molecular switch drives a ring-closing reaction through visible light, and the molecular switch has high visible light responsiveness and light isomerization conversion rate.

The present invention relates to a capsule luminescence pigment comprising a luminescent substance and a photochemically switchable absorber as a molecular switch, wherein the luminescent substance and the photochemically switchable absorber are dissolved and / or dispersed as separate molecules in a polymer particle, the luminescent substance has an excitation spectrum, the photochemically switchable absorber has a first absorption spectrum in a first switching state and a second absorption spectrum in a second switching state, the first absorption spectrum of the photochemically switchable absorber does not significantly overlap with the excitation spectrum of the luminescent substance, and the second absorption spectrum of the photochemically switchable absorber significantly overlaps with the excitation spectrum of the luminescent substance, so that in the first switching state significantly more intense luminescence of the luminescent substance occurs than in the second switching state of the photochemically switchable absorber.The present invention further relates to a switchable luminescent printing ink comprising a capsule luminescent pigment as described herein and to a security document, preferably a banknote, comprising an imprint with a first switchable luminescent printing ink as described herein.

The application provides a DNA enzyme triple helix molecular switch detection system suitable for a personal blood glucose meter, and a preparation method and application thereof, and belongs to the technical field of biosensors.The DNA enzyme triple helix molecular switch comprises an aptamer containing two nucleotide arm segments and DNA enzyme covalently coupled with a magnetic bead; the two nucleotide arm segments of the aptamer form a triple helix structure with the DNA enzyme; and the two nucleotide arm segments comprise a 5' end nucleotide sequence SEQ ID NO.1 of the aptamer and a 3' end nucleotide sequence SEQ ID NO.2 of the aptamer.The application uses a terminal fixation strategy to limit the flexibility of the original aptamer, stabilize the spatial conformation thereof, improve the affinity of the aptamer to the target, utilize the triple helix molecular switch containing the DNA enzyme and the nano-enzyme-gold nanoparticles, and realize accurate and sensitive detection of trace pollutants in food through determination of the reduction range of the glucose concentration in the detection liquid by using the household blood glucose meter.

The application discloses a kind of multiple stimulus response type Zn (II) -DASA complex molecular switch and preparation method and application thereof.The Zn (II) -DASA complex molecular switch is prepared by grafting DASA ligand with salicylaldehyde acylhydrazone and ZnCl2 in methanol, and crystal is obtained by solution evaporation method.The Zn (II) -DASA complex molecular switch of the application exhibits multifunctional switching characteristics of solid-state light, heat and water-induced color change and fluorescence emission, and has wide application prospect in the field of information storage medium such as photo-thermal color-reproducible erasable material, intelligent display material or anti-fake encryption material.

The invention discloses an EVOH packaging material with long-acting antibacterial and antioxidant functions and a preparation method of the EVOH packaging material, wherein the EVOH packaging material is obtained on the basis of a dynamic molecular switch and a microcapsule targeted release technology. The material comprises an EVOH matrix (A), an environmental response type molecular switch polymer (B) and an intelligent microcapsule (C) sensitive to at least one putrefying biomarker, and a compatibilizer (D). In a normal state, the molecular switch is turned off, and the release of the active agent is inhibited; when the environment humidity is increased or the concentration of the putrefying biomarker is changed, the molecular switch is turned on, and the microcapsule is disintegrated in a targeted manner, so that the efficient and intelligent release of active substances is realized. The material can prolong the effective period of an active agent by more than 300%, realizes accurate intervention on food spoilage, and is suitable for the field of high-end intelligent packaging.

The application discloses a HAT probe with adjustable switch characteristics and application thereof, which comprises a secondary structure capable of forming specific steric hindrance, the secondary structure is a convex "bubble" or "ring", and the core of the secondary structure is a polydeoxythymidine sequence with a length of 5-30 nucleotides. The HAT probe has a "switch" regulation function: a molecular switch is constructed by utilizing the inhibition and activation of Cas12a activity of the complete structure and the linear structure after cutting of the HAT probe. The "switch" can respond to specific biomolecules (such as miRNA, protein), and convert the existence of the biomolecules into a detectable signal.

The invention relates to the technical field of high-value utilization of marine biological resources, and discloses a high-activity marine biological peptide prepared by an enzymolysis method and application of the high-activity marine biological peptide in food. The target high-activity marine biological peptide and an affinity molecular switch sensitive to the concentration change of the target high-activity marine biological peptide are jointly fixed on a magnetic nano-carrier with bitter peptide adsorption functional groups on the surface to obtain the intelligent enzyme carrier; and adding the intelligent enzyme carrier into a reaction system containing a marine protein raw material and a first amphiphilic microcapsule wall material to carry out an enzymatic hydrolysis reaction in the reaction process. By constructing an intelligent enzyme carrier integrated with a directional enzyme and an affinity molecular switch, precise cutting and process self-control of an enzymolysis reaction are realized, and the affinity molecular switch can respond to the change of the concentration of the high-activity marine biological peptide and spontaneously adjust the enzyme activity, so that excessive degradation of a target product is effectively avoided.

The application discloses a reversible single-molecule switch based on local cation regulation and belongs to the technical field of molecular electronics. The application is based on the crack junction technology of an electrochemical scanning tunneling microscope. The distribution of local cations in an interface double electric layer is changed by controlling an electric potential, so that the state of a carboxylic acid molecule is influenced. The contact action of the carboxylic acid molecule and a gold needle tip is adjusted, and a single-molecule switch is realized. The electric potential is-0.5-0V. The application proves the influence of local cations in an electrochemical interface on carboxyl molecules, and opens up a new way for realizing the practical application of a reversible single-molecule switch through a gate electrode.

The application discloses a ClHAK8 gene promoter variation site related to watermelon salt tolerance and application thereof, and relates to the technical field of biology.The ClHAK8 gene promoter variation site is located at the position of-1534 to-1529 bp in the ClHAK8 gene promoter region; and the nucleotide sequence of the ClHAK8 gene is shown as SEQ ID NO.1.It is first clarified in the application that the presence or absence of the W-box element (TTGACC) at the position of-1534 to-1529 bp in the upstream promoter region of the watermelon ClHAK8 gene is a core molecular switch for regulating watermelon salt tolerance.The application clarifies the molecular mechanism of the variation of the promoter cis-element for affecting the salt stress response of watermelon by regulating the expression of a potassium transport gene, perfects the salt tolerance regulation network of watermelon, and provides a stable and practical detection tool for the molecular marker assisted breeding of salt-tolerant watermelon.

The present invention relates to the field of lysosome imaging probes and optical super-resolution imaging. A light-controlled fluorescent molecular switch targeting lysosomes has a structural formula as shown in Formula I: wherein R is any one of NMe2 and NEt2.

The invention relates to the technical field of molecular switches, and discloses an optical switch molecule and a preparation method and application thereof. The structural general formula of the optical switch molecule is as follows: R1 is a hydrogen bond acceptor group, R2 is an electron donating group or an electron withdrawing group, and R3 and R4 are spatial substituent groups. According to the invention, sp3 hybrid carbon atoms are used as bridging units, and a series of space folding type ESIPT optical switch molecules are constructed for the first time and are applied to the aspects of chemical sensing, information encryption, data storage and the like. According to the method, light is used as an external stimulation condition to induce the photoswitch molecules to generate a rapid six-level photophysical-chemical process and generate a cis-trans isomerization process in an excited state, so that the photoswitch molecules are macroscopically represented as high-contrast color change before and after photostimulation.

The present invention relates to automotive products, as well as uses thereof, which can facilitate warming of engine oil in an energy efficient and, therefore, more environmentally friendly manner. More specifically, a fluid reservoir is disclosed that includes a double walled vessel that defines a fluid containing compartment and has a self-contained region that shares a wall with the fluid containing compartment. A composition in the self-contained region at least partially covers the shared wall surface, and one or more light sources are positioned in the self-contained region to induce a phase change in the composition. The composition includes a phase-change material (PCM) and a molecular switch material. This fluid reservoir can be used to heat oil in an engine or oil pan.

The invention belongs to the technical field of gene and agricultural product storage, and discloses a PRX3 gene and application of acetylation modification of the PRX3 gene in regulation and control of dormancy and / or oxidation, the PRX3 gene is StPRX3, and the gene sequence of the PRX3 gene is shown as SEQ ID NO.1. The positive regulation effect of the deacetylation protease (StPRX3) on the dormancy of the potatoes in the storage period is confirmed for the first time by integrating bioinformatics prediction and an instantaneous conversion technology. A technical path for accurately regulating and controlling the dormancy process through a molecular means is also established, the uncertainty influence of environmental factors on the tuber quality in a traditional storage mode is effectively reduced, a key molecular switch for connecting apparent modification and redox regulation and control is determined, and a new thought is provided for preservation of the harvested potatoes.

Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

This invention belongs to the field of photonics, functional optical materials and devices, specifically relating to a spectral dynamic programming device, its fabrication method, and its applications. The spectral dynamic programming device has a three-layer structure, comprising, from bottom to top, a light-emitting thin film, a cholesteric liquid crystal layer, and a circular polarization analyzer. The light-emitting thin film is a fluorescent thin film or an organic room-temperature phosphorescent polymer thin film; the cholesteric liquid crystal layer is a mixture of a photoresponsive chiral molecular switch, a static inverse chiral dopant, and a nematic liquid crystal host. By alternately irradiating the liquid crystal layer with light of different wavelengths, the helical pitch of the liquid crystal layer can be reversibly and continuously adjusted, allowing its photonic bandgap reflection band to sweep across a wide spectral range. This dynamically "tailors" the emission spectrum of the lower light-emitting thin film, achieving continuous and reversible programming of the output color. This device can also generate emission with a high circular polarization asymmetry factor and possesses excellent cycling stability, making it applicable to the optical field.

Provided herein is a nucleotide cassette comprising an inducible promoter, a nucleotide sequence corresponding to at least one single-stranded RNA diagnostic target, a nucleotide sequence encoding a neurilemmal, a molecular switch, and a nucleotide sequence encoding a DNA enzyme under the control of the molecular switch, wherein the single-stranded RNA diagnostic target is a sequence detected by a molecular diagnostic assay. In some embodiments, the nucleotide cassette can be used to obtain an RNA expression product. Also provided are vectors and cells comprising the nucleotide cassette or its RNA expression product. The nucleotide cassette can also be used to obtain a diagnostic control composition comprising a non-pathogenic recombinant bacterium and a method for producing such a recombinant bacterium, wherein the non-pathogenic recombinant bacterium has a modified genetic content comprising the nucleotide cassette.

This invention discloses a system, method, and reagent kit for detecting biochemical oxygen demand (BOD) in wastewater, belonging to the field of environmental monitoring technology. The detection system of this invention includes at least two independent detection channels and a signal processing module. Each detection channel independently includes an enzymatic conversion module, which contains hydrolytic enzyme systems, oxidoreductase systems, and coenzyme NAD for specific types of organic matter. + The signal transduction and amplification module includes a CRISPR-Cas effector protein, a fluorescent reporter probe, and a molecular switch. The signal processing module detects the fluorescence signal intensity of each detection channel and converts the fluorescence signal into the biochemical oxygen demand (BOD) contribution value of each detection channel based on a pre-stored standard curve, summing them to obtain the total BOD. This invention effectively overcomes the detection uncertainty caused by fluctuations in microbial activity and differences in substrate degradation rates in traditional methods, significantly shortening the detection cycle to within 30 minutes.

The application provides a method for building a 9-bit parity check molecular switch circuit, and the steps are as follows: based on DNA strand displacement technology, the structure and reaction mechanism of the molecular switch circuit are described; the reaction rate and leakage rate are set, and the input signal of the molecular switch circuit and the concentration ratio of the molecular switch at each stage are optimized; the fan-in molecular switch circuit and the fan-out molecular switch circuit are set according to the molecular switch circuit; the 3-bit parity check molecular switch circuit is built based on the fan-in molecular switch circuit and the fan-out molecular switch circuit by using the switch canvas routing strategy, the 9-bit parity check molecular switch circuit is built by using three 3-bit parity check molecular switch circuits with the same structure; and the correctness of the 9-bit parity check molecular switch circuit is verified by using a Simbiology simulation platform. In the construction of a large-scale complex logic circuit, the application has a simple and unified DNA chain structure, a fast reaction speed and good stability, and provides a new scheme for the construction of a modular DNA circuit.

The present invention relates to a capsule-luminescent pigment comprising a luminescent substance and a photochemically switchable absorber as a molecular switch, wherein the luminescent substance and the photochemically switchable absorber are dissolved and / or dispersed as separate molecules in a polymer particle, the luminescent substance having an excitation spectrum, and the photochemically switchable absorber having a first absorption spectrum in a first switching state and a second absorption spectrum in a second switching state, wherein the first absorption spectrum of the photochemically switchable absorber does not significantly overlap with the excitation spectrum of the luminescent substance and the second absorption spectrum of the photochemically switchable absorber does significantly overlap with the excitation spectrum of the luminescent substance, so that the luminescence of the luminescent substance is considerably more intense in the first switching state of the photochemically switchable absorber than in the second switching state of the photochemically switchable absorber. The present invention also relates to a switchable luminescent printing ink, comprising a capsule-luminescent pigment as described herein, and to a value document, preferably a banknote, comprising a print having a first switchable luminescent printing ink as described herein.

The invention relates to an encapsulated luminescent pigment comprising a luminescent substance and a diarylethene as a molecular switch, wherein the luminescent substance and the diarylethene are dissolved and / or dispersed as separate molecules in a polymer particle. The luminescent substance has an emission spectrum, and the diarylethene has a first absorption spectrum in a first switched state and a second absorption spectrum in a second switched state, the first absorption spectrum of the diarylethene not significantly overlapping with the emission spectrum of the luminescent substance and the second absorption spectrum of the diarylethene significantly overlapping with the emission spectrum of the luminescent substance such that the luminescence of the luminescent substance is substantially more intense in the first switched state than in the second switched state of the diarylethene. The invention further relates to a switchable luminescent printing ink, comprising an encapsulated luminescent pigment as described herein, and to a value document, preferably a banknote, comprising an imprint which has a first switchable luminescent printing ink as described herein.

The application discloses a preparation method of a stimulus-responsive anti-pollution easy-to-clean nanofiltration membrane and belongs to the technical field of nanofiltration membranes. In the application, an amino monomer containing a dynamic disulfide bond (S-S) is used as a molecular switch, the disulfide bond can be reversibly broken into a mercapto group (-SH) under the action of a reducing agent and re-oxidized into a disulfide bond under the action of an oxidizing agent, the disulfide bond is introduced into a polyamide separation layer through an interfacial polymerization technology, and a membrane pore dynamic regulation mechanism of "reduction expansion-oxidation shrinkage" is constructed. When the membrane is polluted, the membrane pores are expanded through reduction treatment, and the pollutants are physically detached; then, the membrane pore structure is restored through oxidation treatment, and self-cleaning is realized.

The application is a divisional application of the Chinese invention patent application with application number 202410914209.2, application date of 2024.07.09 and invention name of "Molecular switch for controlling the transformation of colorectal cancer cell genome from MSS to MSI, inhibitor and application thereof". The application discloses the application of verteporfin or VT103 in reducing the level of MMR protein, and belongs to the technical field of medicine. The application finds that verteporfin and VT103 can transform the microsatellite stable state of colorectal cancer cell genome into a microsatellite unstable state, induce the tumor cells with microsatellite instability to exhibit reduced expression of mismatch repair proteins, increased mutation load and increased neoantigen load, can increase the mutation frequency of tumor cells, stimulate the generation of tumor neoantigen peptides, realize the transformation from "cold" tumor cells that cannot respond to immunotherapy to "hot" tumor cells, and provide a theoretical basis for the development of drugs for microsatellite stable digestive tract tumors and the treatment strategy of microsatellite stable tumors by targeting YAP / TAZ-TEAD combined with immunotherapy.