35 results about "Nucleotide base sequence" patented technology

A novel phasing algorithm harnesses sequencing read information from next generation sequencing technologies to guide and improve local haplotype reconstruction from genotypes. Techniques include determining correlated occurrences of single nucleotide polymorphisms (SNPs) in genes of a population of individuals. A plurality of sequences of nucleotide bases in one or more individuals from the populations of individuals is determined based on ultra-high throughput sequencing of a sample from the one or more individuals. Haplotypes included in the population of individuals are determined based on both the correlated occurrences and the plurality of sequences. The inclusion of paired end read data is especially advantageous for the phasing of rare variants, including singletons.

The invention belongs to the fields of a gene sequence and an obtaining method of the gene sequence, and particularly relates to an obtaining method of an SNP (single nucleotide polymorphism) site and a CAPS (cleaved amplified polymorphic sequence) mark interlocked with a citrullus lanatus fruit bitter taste gene Bt (bitterness). The SNP site and the CAPS mark interlocked with the citrullus lanatus fruit bitter taste have nucleotide base sequences in SEQ ID NO:1-2 in a sequence table. The citrullus lanatus fruit bitter taste gene is primarily positioned by screening a bitter taste single plant in an RILs colony and combining with the high-density genetic map information of the citrullus lanatus; the candidate SNP site interlocked with the character is combined with separate colony verification analysis and natural colony verification analysis; the mark tightly interlocked with a target character is obtained; the SNP site and the CAPS mark can be applied to improvement of citrullus lanatus variety and molecular assisted breeding; a technical support is provided for molecular breeding of the citrullus lanatus quality; and meanwhile, the traditional gene positioning time is greatly shortened.

The invention relates to the fields of gene sequences and acquiring methods thereof, and concretely relates to SNP loci linked with a blight resistant gene Fon-1 in a watermelon, markers thereof, and acquiring methods of the loci and the markers. The SNP loci linked with a blight resistant gene Fon-1 in a watermelon and the markers thereof have nucleotide base sequences represented by SEQ ID NO:1-8 in a sequence table. The acquiring method of the loci comprises the following steps: 1, selecting a tested material; 2, carrying out preliminary positioning of the blight resistant gene Fon-1 in the watermelon; 3, acquiring candidate SNP; and 4, acquiring the SNP loci closely linked with the blight resistant gene Fon-1 in the watermelon, and verifying the candidate SNP loci. According to the invention, the phenotype identification and the molecular detection of F2 generation separation populations and natural populations are carried out through utilizing a series of markers to confirm the close linkage degree of the series of the SNP loci with target properties; and the SNP loci are utilized to design the markers respectively, and the markers can be utilized to carry out the initial-stage screening of the kind in order to reach a molecule assisted breeding purpose, so the breeding period is substantially shortened, and an important scientific research meaning is possessed.

The invention discloses a PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3, and belongs to the technical field of agro-biological engineering. According to the PCR molecular marking method, four specific molecular marking primers are designed and synthesized for PCR amplification of DNA of different rice based on the difference of long-grain and large-grain variety TD70 and short-grain and small-grain variety Kasalath on the nucleotide base sequence of the long-grain gene qGL3, the amplified product is subjected to electrophoresis detection with 2.0% of agarose gel, and DNA brands of different dimension represent different allele types of qGL3 after DuRed nuclear acid developing. With the adoption of the molecular marking method, the difference of different alleles of the long-grain gene qGL3 in rice germplasm resources or breeding population can be quickly and accurately identified; in addition, the selection efficiency for the long-grain homozygous genotype qGL3-TqGL3-T is obviously improved; and therefore, the coordinative improvement of the appearance quality and output traits of rice can be realized.

The present invention relates to a system and a method for quickly and accurately identifying and classifying resistance genes of a plant from a protein or DNA sequence. In order to identify and classify resistance genes of a plant using a hidden marcov model, conceived is a profile matrix made using a protein sequence of a domain which is encoded by the resistance genes, and a system for identifying the domain of the resistance genes using the profile matrix and classifying the resistance genes by domain combination. The present invention enables effective identification and classification of the resistance genes of a plant using the profile matrix and program, of which the nucleotide base sequence or protein sequence is detected.

The invention discloses a CAPS (cleaved amplified polymorphic sequence) molecular marking method for identifying solanum lycopersicum with purple black striped pericarp and an application. The sequence of a molecular marker carried by the solanum lycopersicum with purple black striped pericarp is a nucleotide base sequence shown as SEQ ID NO.1, PCR amplification is performed with genomic DNA of the solanum lycopersicum with purple black striped pericarp as a template, nucleotide base sequences shown as SEQ ID NO.3-4 are amplification primers, amplification and cleavage are performed, a specific band different from other solanum lycopersicum pericarp colors can be obtained after cleavage, and the molecular marking method is applied to breed improvement and breeding of the solanum lycopersicum. Compared with the prior art, the CAPS molecular marking method has the advantages that the polymorphism of a cleaved fragment is observed through PCR amplification, cleavage and gel electrophoresis separation, the step of membrane transfer in the traditional RFLP (restricted fragment length polymorphism) analysis is omitted, the operation is simplified, the precision of the RFLP technology is kept, the polymorphism detecting probability is higher, the solanum lycopersicum with purple black striped pericarp is obtained rapidly and applied to breed improvement and assisted breeding, and the method has important theoretical and practical meanings for culturing new varieties of solanum lycopersicum containing rich anthocyanidin.

The invention discloses a novel MAR (matrix attachment region) core fragment-containing animal cell expression vector. The nucleotide base sequence of the MAR core fragment of the recombinant expression vector is as shown in SEQ ID No. 1, SEQ ID No.2 or SEQ ID No. 3. The expression vector disclosed by the invention can effectively increase the yield of protein in mammalian cells and reduce production cost.

The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

The invention belongs to the field of gene engineering, and particularly relates to a molecular marker GI1354 closely linked with a pepper CMV resistance gene and an acquisition method and application. The molecular marker GI1354 has at least one of nucleotide base sequences shown in SEQ ID NO:1 and SEQ ID NO:2 in the sequence list. During detection of the cr gene in the molecular marker application pepper material, the steps of extraction of genome DNA of the pepper material, the PCR amplification reaction, analysis and the like are included. The screened PCR primer is directly used as the molecular marker, whether the cr gene is contained in the pepper material or not can be accurately detected, and the method is convenient to implement, rapid and capable of saving cost.

The invention belongs to the technical field of molecular biological detection and in particular relates to a pathogenic detection kit for a lentivirus of a small ruminant. The kit comprises (1) a genetic marker for detecting the lentivirus of the small ruminant, wherein the genetic marker has a sequence as shown in SEQ ID NO.3 or other sequences, the sequence consistency of which is greater than 80%; (2) a specific primer pair for amplifying the genetic marker, the specific primer pair comprising an upstream primer sequence as shown in SEQ ID NO.1 and a downstream primer sequence as shown in SEQ ID NO.2 or other sequences, the consistency of any continuous eight nucleotide base sequences of which is greater than 80%, among the upstream and downstream primer sequences; and (3) a kit for detecting the lentivirus of the small ruminant, wherein the kit comprises the specific primer pair in (2).

The invention discloses a GhRFP1 gene and a recombinant vector thereof. The nucleotide base sequence of the gene is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO.2. Accordingto the novel gene GhRFP1 and the recombinant vector provided by the invention, after the gene GhRFP1 is introduced into cotton, the length of cotton fibers is increased.

A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterized by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets.

Derivatives of 7-deaza-2′-deoxyguanosine are described. Also described is the use of such compounds in synthesis of nucleic acid polymers and in methods for determining a nucleotide base sequence.