63results about How to "Increase hybridization rate" patented technology

The invention relates to a microfluidic device with microchannels that have separated regions which have a member of a specific binding pair member such as DNA or RNA bound to porous polymer, beads or structures fabricated into the microchannel. The microchannels of the invention are fabricated from plastic and are operatively associated with a fluid propelling component and detector.

The invention discloses a method for promoting the hybridization of willows, which comprises the steps of collecting female flower branches, culturing the flower branches, pollinating and the like. In the invention, the hybridization place is centralized, and the management is convenient; the hybridizing technique is easy, and large-scale operation can be realized; flower forcing is performed by using carbon dioxide, the blooming time of female flowers can be advanced to promote the success of the hybridization; and the hybrid offspring is cultured in nutritional solution, full seeds can be produced, and the fruiting rate of the hybridization can be improved by over 80 percent. When the method is used, the hybridization breeding process of willows can be effectively accelerated, and hybrid seeds of willows can be obtained within 15 to 30 days.

The invention relates to an artificial hybridization type petal pollination method for a tea tree, and belongs to the technical field of cross breeding methods. The artificial hybridization type petal pollination method is characterized by comprising following steps of (1) confirming an object to be pollinated; (2) collecting pollen; (3) drying the pollen and storing; (4) gathering the pollen; (5) castrating; (6) pollinating petals; (7) bagging; and (8) removing bags. When the efficient artificial hybridization is performed in a land for growing field crops, as the method is adopted, the pollination speed is high and the use ratio of the pollen is high, the pollen consumption amount is saved, and compared with conventional pollination methods, for the method disclosed by the invention, the use ratio of the pollen and the pollination speed are increased by more than two times, the method is simple to operate; through the petals are relatively soft, the stigma can not be damaged; the hybridization maturing rate is increased.

The invention relates to a method for improving the hybridization fructifying rate of wheat and elytrigia repens, which comprises the following steps: selecting a shortest stalk sterile plant of wheat as a female parent and selecting elytrigia repens as a male parent to be hybridized; planting the elytrigia repens in a greenhouse at 23 DEG C-28 DEG C from August to September and heating and irradiating the elytrigia repens at the temperature lower than 15 DEG C in winter for 10 hours / day of illumination time to enable the elytrigia repens to bloom and scatter pollen in late April of the next year; transplanting the shortest stalk sterile plant of the wheat in a flowerpot to be placed under an elytrigia repens plant and cutting one third of glume to enable a stigma of the shortest stalk sterile plant to be exposed after growth; and leading the pollen of the elytrigia repens to freely fall on the stigma of the shortest stalk sterile plant of the wheat during pollen scattering. The invention solves the problems of difficult hybridization and low fructifying rate between common wheat and the elytrigia repens, saves power and time and has high efficiency and can transfer good genes of the elytrigia repens in the common wheat at maximum so as to synthesize novel species and types.

The invention discloses a wheat hybridizing method which comprises the following steps: selecting a strongly grown main spike on a mother plant, removing stunted spikelet at the base part and the upper part of the main spike of the mother plant, and retaining a first small flower and a second small flower at both sides of each stunted spikelet so as to finish the spike trimming process; cutting off one third of the first small flower and the second small flower to enable three anthers of the first small flower and the second small flower to be level to glumes so as to finish the glume cutting process; removing the three anthers of the first small flower and the second small flower to form stigmas and sleeving a transparent glass paper bag on the main spike of the mother plant so as to finish the castration process; standing for 4 days-8 days and pollinating the grown stigmas in a spike twisting way so as to finish the pollinating process. The inner glume and the outer glume do not need to be pressed in the castration process in a glume cutting way, and the invention has the characteristics of simple and convenient operation, easy mastering, high purity of harvested wheat hybridized seed, good effect, and the like.

The invention relates to a rapid seed selection method of a high-anthocyanin purple sweet potato variety and belongs to the technical field of crossbreeding. The rapid seed selection method comprises the steps that F1-generaiton hybrid true potato seeds are obtained, a citric acid- disodium hydrogen phosphate buffer solution extraction colorimetric method is used for measuring the content of anthocyanin in sweet potato tubers, single plants of fresh tuber with the content of anthocyanin being larger than or equal to 70 mg / 100 g are selected, and rapid breeding and seed breeding are conducted. Because selection according to the index of the content of anthocyanin is conducted in the first step, the materials with the content of anthocyanin being smaller than 70 mg / 100 g are weeded out, and it is guaranteed to the maximum extent that the high-anthocyanin single plants in the filial generation are selected; because the number of single plants selected according to the citric acid- disodium hydrogen phosphate buffer solution extraction colorimetric method is remarkably smaller than that of single plants selected according to a method for observing the color depths of sweet potato pulp with eyes, the number of single plants needing following field authentication is greatly reduced, and the workload is remarkably lightened; a different-place multi-point yield comparison test can be completed in a one-off mode, and the seed breeding time is shortened by 2-3 years.

The invention discloses a method for breeding a chromatic potato variety with high pigment content. The method comprises the following steps: determining the pigment content; analyzing the genetic relationship; carrying out mixed pollination; screening cross generations; carrying out field screening and pigment evaluation; carrying out variety comparison testing and regional testing on screened later generations which are high in pigment content, and breeding a new chromatic potato variety with high pigment content and good agronomic traits. According to the method disclosed by the invention,the proportion of strains, which are high in the pigment content, in the cross generations can be remarkably increased, and the selecting efficiency of breeding a new variety with high pigment contentcan be increased; the genetic relationship of cross parents of the chromatic potatoes can be analyzed by utilizing molecular markers, materials having a distant genetic relationship can be selected for pairing and hybridizing, and the agronomic traits such as the pigment content, the quality and the yield of the cross generations are increased by utilizing heterosis; the mixed pollination is adopted, the hereditary basis is expanded through a multiple male parent pollination strategy, and the variation types of the cross generations are added while the cross fertile rate is increased.

The invention discloses an artificial hybridization method for millet. The method comprises the following steps: 1) for ears of maternal plants of millet with the total number of flowers accounting for 10 to 15% of the total number of spikelets, removing a part of seed strings of the ears until each of the ears has 15 to 25 seed strings and removing all the blossomed flowerets and unfully-grown flowers in the retained seed strings; 2) wetting the ears of the maternal plants processed in step 1), wrapping the ears of the maternal plants to allow the ears to be in an environment with a temperature of 23 to 30 DEG C and relative air humidity of more than 95% and carrying out emasculation and pollination after 18 to 21 h; and 3) culturing pollinated plants under the condition that the ears ofthe maternal plants are isolated from external pollen and collecting seeds. According to the invention, a method of moisture retention and emasculation through wrapping is employed; therefore, cracking of anther of the maternal plants is effectively prevented, working time for emasculation can be prolonged, and mechanical damage to stigma during manual emasculation is avoided.

The invention discloses a male sterilant used for head cabbage, and a preparation method thereof. The finished product of the prepared male sterilant used for the head cabbage contains tribenuron-methyl and gibberellin (GA3); and the preparation method comprises the following steps: the tribenuron-methyl solution and the gibberellin (GA3) solution are prepared, and then the tribenuron-methyl solution and the gibberellin (GA3) solution are evenly mixed and then are fixed on volume to obtain the finished product. The male sterilant has the effects of thorough male-killing, complete sterility of the stamen, normal growth and development of the plants and normal pollination of the insects; the crossing rate of the produced first generation of hybrid seeds reaches 100 percent; the preparation technique is simple, and the bath production is easy; when the maximum length of the flower bud of the main scape or the first-order branch of the plant of the cabbage is about 2 mm, the spraying period is selected, the whole plant is sprayed by a compression hand-held sprayer, and each plant is sprayed with 15 ml of working fluid.

The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

The invention belongs to the technical field of crop cultivation, and provides a method for increasing the quinoa hybridizing rate for solving the problems that due to the fact that quinoa flowers are small, complex and not suitable for emasculating, very big obstacles are brought to the quinoa breeding work. Hybrid seeds are obtained through the steps of greenhouse planting, parent selecting, top-end flower bud treating, isolating, emasculating and pollinating, and the problems that the quinoa flowers are small and difficult to emasculate are solved by adopting a magnifying lens to assist an inoculating loop technology. Microscopic observation and actual operation effects determine that the optimal pollinating time is about 9:30 am and 3:00 pm. Living body pollinating is performed by adopting a 3:1 mode, that is, three male parents and a female parent are adopted, the pollen activity is high, and the flower season duration time is long. Pollinating is performed by adopting a vibrating rod indirect contact method, and compared with a traditional finger picking method and vibrating method, the regularity is high, the controllability is better, the vibrating amplitude is small, the strength is high, shed pollen is large in amount and does not drift away all round, the stigma pollinating rate is higher, and the hybridizing rate reaches 35%-40%.

The invention discloses a crossbreeding method of common kidney beans, and relates to the field of crossbreeding. The crossbreeding method comprises the following steps: picking the anther and stigma of a male parent carina peridium simultaneously; arranging carina picked from a male parent in a female parent stigma through a small patulous opening in a sleeving manner. The crossbreeding method has the following benefits: 1, the workload is decreased: the anther and stigma of the male parent carina peridium are directly picked simultaneously, so that the steps of obtaining male parent pollen are simplified, and the workload is decreased; 2, the seed setting index is increased: the technical key point is based on the male parent, the male parent carina is complete, all the pollen is in the carina, pollen loss is avoided, the complete male parent carina provides an optimal environment for the female parent stigma, and the optimal environment is similar to the stigma self-pollination environment in a female parent carina, so that water loss of the female parent stigma is reduced, and the probability of pollination success is increased; 3, the hybridizing rate is increased: the female parent stigma is sleeved by the male parent carina, the female parent stigma is enabled to be in contact with the male parent pollen only, and other pollen is isolated, so that the hybridizing rate is guaranteed.

The invention provides a breeding method of intraspecific hybridization of black shrimp, comprising the steps of collecting and separately cultivating excellent local population, selecting female shrimps and male shrimps with sexual maturity and cross mating with excellent seed shrimps in different places. The breeding method of the invention has the remarkable advantages in the hybrid rate and the economical rate of male parent, etc; compared with the existing shrimp larvae, the shrimp larvae cultivated by the breeding method of the invention has remarkable progress in such aspects as growth rate, ability of resisting diseases and inverse and delaying sexual maturity, etc, fully shows the heterosis, and improves the commodity rate, individual specification and economic benefit of shrimp cultivation.

The invention relates to a detection reagent and a kit for detecting male-sterility. Concretely, the detection reagent comprises 1) at least one of a GLP DAZ a probe set and a GLP DAZ b probe set, and 2) a CSP 18 probe set. The kit comprises the detection reagent dissolved in a TE Buffer, wherein each one of the probe sets in the kit has concentration of 50 to 300 ng / microliter. The invention also relates to a use of the detection reagent and the kit in detection of DAZ gene family deletion, and a method for detection of DAZ gene family deletion.

The invention discloses a large-field pepper hybrid seed production method and belongs to the technical field of plant hybrid seed production. The large-field pepper hybrid seed production method is used for overcoming conditions of great labor wastes, low seed production and plenty hybrid seeds in the existing large-field pepper hybrid seed production process. The large-field pepper hybrid seed production method comprises the following specific steps: (1) collecting and treating pollen; (2) castrating; (3) pollinating; (4) marking labels; and (5) performing hybridization aftertreatment and harvesting. The large-field pepper hybrid seed production method has the characteristics of low pollen consumption, convenient castration, simple pollination, simple marking and convenient allocarpy confirmation during harvesting, and is capable of greatly reducing the labor consumption during the large-field pepper hybrid seed production process and improving the hybrid seed purity.

The invention discloses a method for improving the hybridization efficiency of wutah, which belongs to the field of horticultural technology. It mainly aims at the labor-intensive, time-consuming and low-efficiency characteristics of artificial hybridization of Wutaicai, and improves the hybridization efficiency of Wutaicai through isolation, reasonable assembly, bagging, and removal of shriveled seeds. The invention has the characteristics of simple operation, low cost, good effect and the like.

The invention relates to an emasculation method for sunflowers, particularly to an artificial emasculation method for sunflower hybridization and is applied to fields of artificial emasculation and hybridization of the sunflowers. The artificial emasculation method for the sunflowers is convenient and quick to operate, capable of saving manpower and material resources and good in emasculation effect. The artificial emasculation method for the sunflowers comprises the step as follows: when stamens and pistils of the sunflowers are separated at a certain distance, removing anther with bent-head tweezers. The artificial emasculation method for the sunflowers is convenient to operate and high in efficiency, the anther can be removed effectively, and the hybrid rate can be increased, so that resources are saved, and the cost is reduced.

The invention provides a breeding method of intraspecific hybridization of black shrimp, comprising the steps of collecting and separately cultivating excellent local population, selecting female shrimps and male shrimps with sexual maturity and carrying out the cross mating with excellent seed shrimps in different places. The breeding method of the invention has the remarkable advantages in the hybrid rate and the economical rate of male parent, and the like; compared with the existing shrimp larvae, the shrimp larvae cultivated by the breeding method of the invention has remarkable progressin such aspects as growth rate, ability of resisting diseases and inverse and delaying sexual maturity, and the like, fully shows the heterosis, and improves the commodity rate, individual specification and economic benefit of shrimp cultivation.

The invention discloses a hybridization method for oats. The hybridization method includes the steps of parent selecting, scion finishing, castrating, tassel preparing, bagging and pollination. The parent selecting step is improved, wherein female parents select individuals that 3 / 4 scions ear, male parents select individuals that 3 / 4 scions ear, the quantity of florets after being castrated can be effectively increased after improvement, and therefore the working efficiency is improved; the scion finishing step is improved, wherein 1 / 4 florets are reserved while clipping is carried out duringscion finishing, and the weight of seeds can be effectively increased; the tassel preparing and bagging steps are improved, wherein robust plants are selected in male parent lines, glume is not clipped, and the robust plants are directly inserted into test tubes, and positions of the male parent scions are higher than that of the female parent scions, so that it is guaranteed that pollen of the male parent scions can fall on stigmas of female parent florets from top to bottom. According to the hybridization method, the survival time of the male parent scions can be prolonged, the pollinationtime can be prolonged, the hybridization setting rate is increased, hybridization time can be effectively shortened when the glume is not clipped, and the influence on the hybridization efficiency isavoided.

The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

The invention discloses a method for applying a silkworm moth dark flowery wing gene to domestic silkworm genetic breeding and aims to solve the technical problems of pollution caused by artificial chemical staining technology and low degree of practicality of darkened gene resources. According to the method, the dark flowery wing gene found in practical variety resources is introduced to one variety of a new hybridized combination to distinguish and reject a pure pair by using the silkworm body color quite different from that of another pair of hybrid varieties without reducing the degree of practicality of economic character, so that the aim of improving the hybridization rate of the hybridized varieties is achieved. The method for applying the silkworm moth dark flowery wing gene to the domestic silkworm genetic breeding provided by the invention is simple in procedure, low in cost, free from pollution, good in economic character and high in practical value; in addition, the hybridization rate of the hybridized varieties can be greatly improved by the composition characteristics of the dark flowery wing genes in the new hybridized combination, that is, one is a dark flowery wing, and the other is an ordinary white wing. Therefore, the phenomenon that the whole batch of silkworm eggs is rejected due to the unqualified hybridization rate index is avoided.

The invention discloses an oat crossbreeding method. The method includes steps: selecting different oat varieties as male parents and female parents, culturing the male parents right to an initial flowering stage, and culturing the female parents until 3-5 top spikelets are exposed outside flag leaves; selecting strong plants from the cultured female parents, stripping all spikelets of plants, cutting 1 / 2 of the spikelets, removing kernels and glumes in the spikelets to keep outmost kernels and glumes, removing stamens, and fixing; selecting ears cut from highest joints of the cultured male parents; performing pollination on the ears of the male parents and the female plants in a same transparent bags, continuing culturing, and collecting seeds of the female plants to obtain oat hybrid seeds. By improvement of a hybridization method, a crossbreeding technique suitable for main hereditary characters of oat varieties in China is provided, and the setting percentage of hybrid oat is increased while yield stability and resistance are improved.

The invention discloses a crossbreeding method of high-yield disease-resistant celery. The method comprises the following steps of hybridizing a male sterile line with a maintainer line to obtain a male sterile line combined with excellent properties of a male parent and a female parent, and hybridizing the male sterile line with a restorer line to obtain a celery hybrid. By spraying drugs in theplanting process, bacterial infection is prevented, the disease resistance of seedlings is improved, the seedling stage is shortened, celery blooming is induced, celery plants which are not in the blooming stage are pulled out before cross pollination, the blooming stage is adjusted, the hybridization rate is increased, and celery hybrids which are high in yield, resistant to diseases, good in quality and high in offspring regularity are obtained.

The invention discloses a method for hybrid seed production, flowering period coordination and field seedling preservation for millet. Seedlings are cultured according to different staggered periods and different proportions of male and female parents and at suitable time in the hybrid millet seed production field, and during field planting, the seedlings are cultured at suitable time according toactual production demands. According to the method, by transplanting cultured millet seedlings, the hybrid seed production and flowering period coordination for the millet are achieved, a suitable seedling reservation density is adopted for millet planting, and the problems are solved that the flowering periods of produced hybrid millet seeds are not coordinated, the hybridization rate and the seed production rate are low, seedling emergence is difficult through direct seeding of traditional millet seeds in the field, lacking seedlings and seedlingless ridges are frequently caused, the per unit area yield of the millet in the field is seriously affected, varieties with a long growth period are planted, or the accumulated temperature of millet for multiple cropping is insufficient after preceding crops are harvested, and the planting enthusiasm of farmers is not high.

The invention discloses a tartary buckwheat crossbreeding method. The tartary buckwheat crossbreeding method comprises the steps of selecting two tartary buckwheat varieties with relatively large target character difference as a male parent and a female parent respectively, cultivating the male parent tartary buckwheat until entering an initial flowering stage, cultivating the female parent tartary buckwheat until more than five or more than flower buds with the size of 1.5-2 mm are exposed from the top, and starting hybridization; selecting unpollinated flower buds of robust plants from the cultured female parents for castration; and selecting flowers with cracked anthers and more pollen attached to the surfaces from the cultivated male parents for pollination, carrying out bagging for continuous cultivation, harvesting seeds of the female parent plants, and thus obtaining the tartary buckwheat hybrid seeds. Through a tartary buckwheat bud castration artificial hybridization technology, a tartary buckwheat hybrid variety which is high in yield, high in stress resistance, wide in adaptability, excellent and high in yield is bred.

The invention discloses a comprehensive technology for improving Arachis hypogaea Linn. hybridization efficiency. The comprehensive technology comprises the following steps of: (1) constructing a hybridization pool: adopting a brick-concrete structure for the hybridization pool, taking neutral sandy loam as a lower base layer of the bottom of the hybridization pool, and taking an upper part as a plantation layer; (2) planting a hybridization parent: after hybridization parent Arachis hypogaea Linn. seeds are subjected to coating processing, carrying out plantation processing according to plantation density that two-parent line spacing is 38-42 cm, female parent row spacing is 22-28 cm and male parent row spacing is 13-18 cm; (3) adopting a before-hybridization management technology; (4) carrying out manual emasculation on a female parent; (5) carrying out artificial pollination; and (6) carrying out comprehensive management after pollination: after all combined pollination is finished,before 7:00 every day, manually picking all non-hybrid-pollination flowers on a female parent plant, continuing for 10-15 days, and carrying out proper manual ridging. According to the comprehensivetechnology, which is provided by the invention, for improving Arachis hypogaea Linn. hybridization efficiency, an Arachis hypogaea Linn. hybridization fructification rate can be improved to be about 18% from original 10%, and breeding efficiency is obviously improved.

The invention discloses a compounding agent for efficiently relieving male sterile rice panicle enclosure as well as a preparation method and application thereof. The compounding agent is prepared from gibberellin 3, indole-3-acetic acid, urea phosphate, Tween 20 and azone according to a certain proportion. The preparation method comprises the following steps of (1) proportionally weighing gibberellin 3, indole-3-acetic acid and ethanol; continuously performing stirring to obtain a mixed solution A; (2) proportionally weighing urea phosphate, Tween 20, azone and water; continuously performingstirring to obtain a mixed solution B; (3) sufficiently mixing the mixed solution A and the mixed solution B to obtain the compounding agent for efficiently relieving male sterile rice panicle enclosure. The compounding agent is low in cost; the method can be easily implemented; the operation is simple and convenient; the reverse one section growth of the male sterile rice can be obviously promoted; the male sterile rice panicle enclosure is relieved; the hybrid rice breeding seed yield increase by 80.4 to 195.6 percent can be realized.

The invention relates to a treatment agent for increasing the hybridization setting percentage of unhusked rice and a method and belongs to the technical field of unhusked rice hybridization. The treatment agent for increasing the hybridization setting percentage of unhusked rice comprises a compounded water solution of gibberellins, indoleacetic acid and naphthylacetic acid; the mass concentration of the gibberellins is 10-30mg / L; the mass concentration of the indoleacetic acid is 0.1-0.5mg / L; and the mass concentration of the naphthylacetic acid is 100-150mg / L. With the combination of the gibberellins, the naphthylacetic acid and the indoleacetic acid, the treatment agent provided by the invention is capable of increasing the hybridization setting percentage of unhusked rice.

The invention relates to the technical field of cross breeding of marine economic shellfish, in particular to a breeding method based on three-way cross of argopecten purpuratus, argopecten irradians and Argopecten irradians concentricus. The method comprises the following steps: firstly, carrying out intersubspecific hybridization on argopecten irradians and argopecten irradians concentricus to obtain first filial generation of hybridized scallops of which the fertility is not influenced; performing interspecific hybridization on the obtained first filial generation of scallops and argopecten purpuratus to obtain the first filial generation of interspecific hybridization scallops which normally develop and have remarkable growth and stress resistance advantages, and purifying the three-way filial generation through group mixing; and the new species of the sea-black-black-purple (or black-black-black-black-purple) three-way hybrid scallops with genetic stability and excellent characters can be obtained through continuous breeding. According to the method disclosed by the invention, the excellent characters of the argopecten purpuratus, the argopecten irradians and the Argopecten irradians concentricus can be integrated, so that a new three-way hybrid scallop variety with more remarkable growth and stress resistance advantages is cultivated, and the improved variety preservation and supply capability of the scallop breeding industry is improved.

The invention relates to a method for improving artificial hybridization efficiency of wheat in Huang-Huai-Hai wheat regions by utilizing greenhouses. According to the method, certain varieties and quantities of wheat breeding parents are planted in field and greenhouse conditions, and by utilizing the different wheat breeding times under the two different conditions, the wheat heading and blooming periods are manually adjusted to achieve the aims of prolonging the manual hybridization time of winter wheat, improving the hybridized spike fructification quality, increasing the quantity of crosscombinations obtained in each year and improving the manual hybridization efficiency.