Rapid seed selection method of high-anthocyanin purple sweet potato variety

A high-anthocyanin, purple sweet potato technology, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problem of less attention to parental anthocyanin content, low probability of emergence of a single plant, more manpower and material resources, etc. problem, to achieve the effect of overcoming infertility in the same group, increasing the rate of hybridization and good economy

Inactive Publication Date: 2015-09-30
CROP RES INST SHANDONG ACAD OF AGRI SCI
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Problems solved by technology

There are certain deficiencies in the traditional purple sweet potato hybrid breeding method: First, when matching hybrid combinations, more attention is paid to the yield level and quality traits of the father and mother, and less attention is paid to the anthocyanin content of the parents, and the super-high anthocyanin content of the offspring appears The probability is low; secondly, due to sweet potato self-fertilization and infertility in the same group, the pollination rate of open insect pollination

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  • Rapid seed selection method of high-anthocyanin purple sweet potato variety

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Experimental program
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Effect test

Embodiment 1

[0026] 1. Breeding process

[0027] (1) Hybrid combination configuration

[0028] Hybrid combination 1: Select high anthocyanin sweet potato varieties Lingzi, Shanchuanzi, Nonglin 54, Jizi 01121, Jizi 01214 and Jizi 20058 with anthocyanin content ≥ 50mg / 100g as parents. Parents were pollinated by artificial pollination (referred to as "artificial pollination") and natural pollination by open insects (referred to as "natural pollination") by mixing the parental pollen evenly, and F1 generation sexual hybrid seeds were obtained respectively. The results are shown in Table 1:

[0029] Table 1 Comparison of results of different pollination methods

[0030] project artificial pollination natural pollination Number of blooms (flowers) 3780 4120 number of results 2173 1454 Seed setting rate (%) 57.2 35.8 hybrid seed (grain) 3476 2617

[0031] The results in Table 1 show that the seed setting rate and the number of hybrid seeds harvested...

Embodiment 2

[0048] 1. Breeding process

[0049] (1) Hybrid combination configuration

[0050] Hybrid combination 1: Select high anthocyanin sweet potato varieties Lingzi, Shanchuanzi, Xuzishu 43-14, Jizi 08123, Jizi 08365 and Jizi 08265 with anthocyanin content ≧ 50mg / 100g as parents, and adopt the parents respectively F1 generation sexual hybrid seeds were obtained by artificial pollination after the pollen was evenly mixed and natural pollination by open insects. The results are shown in Table 4:

[0051] Table 4 Comparison of seed setting rate of different pollination methods

[0052] project pollen mix artificial pollination natural pollination of insects Number of blooms (flowers) 4520 3845 number of results 2770 1195 Seed setting rate (%) 61.3 31.1 hybrid seed (grain) 4100 1635

[0053] The results in Table 4 show that the seed setting rate and the number of hybrid seeds differ greatly among different pollination methods, and the seed...

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Abstract

The invention relates to a rapid seed selection method of a high-anthocyanin purple sweet potato variety and belongs to the technical field of crossbreeding. The rapid seed selection method comprises the steps that F1-generaiton hybrid true potato seeds are obtained, a citric acid- disodium hydrogen phosphate buffer solution extraction colorimetric method is used for measuring the content of anthocyanin in sweet potato tubers, single plants of fresh tuber with the content of anthocyanin being larger than or equal to 70 mg/100 g are selected, and rapid breeding and seed breeding are conducted. Because selection according to the index of the content of anthocyanin is conducted in the first step, the materials with the content of anthocyanin being smaller than 70 mg/100 g are weeded out, and it is guaranteed to the maximum extent that the high-anthocyanin single plants in the filial generation are selected; because the number of single plants selected according to the citric acid- disodium hydrogen phosphate buffer solution extraction colorimetric method is remarkably smaller than that of single plants selected according to a method for observing the color depths of sweet potato pulp with eyes, the number of single plants needing following field authentication is greatly reduced, and the workload is remarkably lightened; a different-place multi-point yield comparison test can be completed in a one-off mode, and the seed breeding time is shortened by 2-3 years.

Description

technical field [0001] The invention relates to a rapid breeding method for purple sweet potato varieties with high anthocyanin content, and belongs to the technical field of hybrid breeding. Background technique [0002] High anthocyanin sweet potatoes are rich in anthocyanins, and the potato flesh is purple or deep purple. Scientific research shows that anthocyanins are a kind of strong antioxidant, which can protect the human body from free radical damage, and have anti-mutation and anti-inflammation properties. Efficacy, and anthocyanin is a water-soluble natural pigment, which can replace the synthetic pigment used in the food processing industry, so the market prospect of high anthocyanin type purple sweet potato is very broad. Since 2003, my country introduced and planted the Japanese fresh purple sweet potato variety Norin 54. In 2004, the Japanese varieties Yamakawa Zi and Yaya Purple were used to process and extract anthocyanins. After 2010, a large number of high ...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 王庆美董顺旭李爱贤侯夫云张立明秦祯
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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