829 results about "Anthocyanidins" patented technology

The invention belongs to the technical field of sweet potato breeding, and provides an SNP marker related to sweet potato root color and anthocyanin content and application of the SNP marker, the SNP marker is located at the 25658800th site of a chromosome 10a of sweet potatoes, and the base polymorphism is A / T. When the SNP genotype of the sample to be detected is A / T, the content of anthocyanin in the tuberous roots is high, and the color is purple; when the SNP genotype is T / T, the content of anthocyanin in the tuberous roots is low, and the tuberous roots are non-purple in a large probability. According to the detection method and the kit provided by the invention, the efficiency of identifying the purple sweet potatoes by the developed SNP marker through sweet potato natural population phenotype identification and verification reaches 100%, the efficiency of identifying the non-purple sweet potatoes reaches 85.04%, the color of the sweet potato tuberous roots and the anthocyanin content can be rapidly and accurately detected, and the detection method and the kit have the advantages of being simple and convenient to operate, low in cost and the like, and have wide application prospects. The requirement of large-scale molecular marker-assisted selection can be met.

The invention relates to the technical field of high-barrier environment-friendly packaging films, and discloses a high-barrier environment-friendly packaging film with an antibacterial function, the high-barrier environment-friendly packaging film comprises a base layer, a nano composite barrier layer, a bio-based functional layer and an outer protective layer from bottom to top in sequence, and the layers are tightly attached through a coated starch-based adhesive. According to the high-barrier environment-friendly packaging film with the antibacterial function, good biodegradability of polyhydroxyalkanoate adopted in the base layer film, polycaprolactone which is adopted in the nano-composite barrier layer film and has a biodegradable function and konjac glucomannan in the biological functional layer film are utilized; the composite packaging film can be decomposed into carbon dioxide and water by microorganisms in various environments, has excellent flexibility and processability, can adapt to different packaging requirements, provides basic flexibility, structural support and green and environment-friendly effects for the whole packaging film, and has a wide application prospect. The procyanidine arranged in the outer protective layer film provides stronger antibacterial property and water solubility for the whole packaging film.

The invention belongs to the field of medical instrument coloring agents, and provides a natural anthocyanin endoscope coloring agent. According to the invention, the innovative design of combining a ternary color-stabilizing compound formed by anthocyanin, a co-chromophore and hydroxypropyl beta cyclodextrin with a chitosan sodium alginate polysaccharide system and a composite buffer system is adopted, and the viscosity is controlled to be 200-320 millisecond, the particle size of spray droplets is 40-60 microns, and the thickness of a thin film is 10-30 microns through specific molar ratio compatibility and a genipin crosslinking technology. Through accurate regulation and control, the excellent performance that a double-network thin layer is formed under the calcium ion environment that the absorbance retention rate at 520 nanometers is not lower than 90% and the elution rate is not lower than 90% under the irradiation of hundred thousand lux white light is achieved, the technical problems that a traditional endoscope coloring agent is poor in safety, low in stability and insufficient in mucous membrane contrast ratio are solved, and the wide clinical application value is achieved.

The invention relates to the technical field of toothpaste preparation, in particular to hydrolyzed procyanidine-containing Chinese herbal medicine nursing toothpaste as well as a preparation method and application thereof.The preparation method of the hydrolyzed procyanidine-containing Chinese herbal medicine nursing toothpaste comprises the following preparation steps: adding a humectant, a thickening agent, a surfactant, a sweetening agent and a preservative into deionized water, and uniformly stirring; uniformly stirring to obtain a mixed solution; adding the abrasive into the mixed solution, and uniformly stirring to obtain paste; and adding the active microcapsules into the paste, uniformly stirring, carrying out vacuum degassing, and filling to obtain the Chinese herbal medicine nursing toothpaste containing hydrolyzed procyanidine. The Chinese herbal medicine care toothpaste is fused with hydrolyzed procyanidine, and shows excellent oral care performance. The oral care solution has obvious inhibition capability on a plurality of common oral pathogenic bacteria, has extremely high bacteriostasis rate, and can effectively block bacterial breeding and maintain oral health from the source. Meanwhile, the toothpaste is excellent in deodorization effect and capable of rapidly decomposing oral cavity peculiar smell molecules and remarkably improving oral cavity freshness.

The invention discloses a mint McTTG1 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the mint McTTG1 gene disclosed by the invention is shown as SEQ ID NO.1, the amino acid sequence of the expression protein of the mint McTTG1 gene is shown as SEQ ID NO.2, and the mint McTTG1 gene belongs to WD40 family genes participating in regulation and control of epidermal hair development and synthesis of anthocyanin and procyanidine. Transgenic experiments show that after the McTTG1 gene is over-expressed in the arabidopsis thaliana mutant ttg1-13 with epidermal hair loss and significantly reduced accumulation of anthocyanin and procyanidine, the formation of the epidermal hair on the surface of the mutant can be recovered, and the accumulation of anthocyanin and procyanidine can be promoted. Gene resources are provided for plant variety improvement, and cultivation of new mint varieties is facilitated.

The invention belongs to the technical field of extraction and purification of plant components, and relates to a low-temperature freezing vacuum drying nano processing method for extracting anthocyanin from purple corn, in particular to a method for extracting anthocyanin from corn kernels, cores, straws, roots and stigmas of purple corn. The processing method comprises the steps of pretreatment, concentration, hydroxyl radical treatment, quick freezing, nano crushing and the like. A hydroxyl radical treatment method is adopted, so that harmful substances such as pesticide residues can be efficiently, quickly and thoroughly removed; the effect of extracting the anthocyanin from corn kernels, roots, cores, stems and stigma of purple corn by adopting a low-temperature freezing vacuum drying technology is remarkable, the purification rate is high, and the anthocyanin containing 99% of biological activity is achieved. The nanoscale anthocyanin is processed by adopting jet mill equipment and can be conveniently absorbed by a human body and widely applied to various fields.

The invention belongs to the field of nano-medicine and traditional Chinese medicine, and particularly discloses an antioxidant nano-material based on self-assembly of traditional Chinese medicine and copper ions and a preparation method thereof.The nano-material is prepared from BPCu nano-particles formed by self-assembly of berberine and procyanidine B2 under induction of the copper ions, the BPCu nano-particles are further subjected to surface modification through polyvinylpyrrolidone, and the antioxidant nano-material is prepared. And the stably dispersed PVP-coated BPCu nano structure is obtained. The material is small in particle size, uniform in distribution, good in water solubility and storage stability, excellent in free radical scavenging capacity and catalase-like activity and suitable for adjuvant therapy of oxidative stress related diseases, and the preparation method is easy and convenient to operate, does not need an organic solvent and has good industrialization and disease treatment potential.

The invention discloses application of a plant lactobacillus CCFM1357 to conversion of anthocyanin to generate various active metabolites, inhibition of a cGAS-STING pathway and enhancement of improvement of brain aging, and belongs to the technical field of microorganisms and the technical field of medicines. The phytobacterium plantarum CCFM1357 fermented anthocyanin and the compound preparation thereof provided by the invention have the following effects: (1) a marker for relieving D-gal induced mouse brain senescence and a marker for relieving D-gal induced mouse brain senescence; (2) promoting and relieving D-gal induced mouse histopathologic characterization; (3) relieving D-gal induced mouse neuroinflammation; (4) relieving behavioral characterization of the D-gal induced aging mouse; and (5) a cGAS-STING signal channel is inhibited. The phytobacterium plantarum CCFM1357 fermented anthocyanin and the compound preparation thereof provided by the invention can be used for a product for relieving brain aging, and have a huge application prospect.

The invention belongs to the technical field of microorganisms, and relates to a plant lactobacillus NXU-F1 strain and application thereof. The classification name of the bacterial strain is lactobacillus plantarum, the bacterial strain is preserved in Guangdong Microbial Culture Collection Center on June 20, 2025, and the preservation number of the bacterial strain is GDMCC No.66563. The invention also discloses a preparation method of the bacterial strain. The plant lactobacillus is strong in growth capability, has good adaptive capacity to environment, and can obviously increase the content of antioxidant substances when being applied to plant matrix fermentation. The lycium barbarum and grape compound fermented beverage is prepared by fermenting the lycium barbarum and grape compound fermented beverage, the content of polyphenol, flavone, polysaccharide and procyanidine in the beverage is obviously increased, and the increase of resveratrol and ferulic acid is particularly obvious; meanwhile, ester compounds are increased and aldehyde substances are reduced, so that the beverage is endowed with strong longan fruity flavor, and the flavor of harmonious sour and sweet taste and lingering aftertaste is formed.

The invention relates to the field of aquatic feed, and provides a low-temperature adhesive type aquatic feed particle composition and a preparation method thereof. The composition is composed of puffed soybean meal, flour, fish meal, low-temperature directed enzyme, microcrystalline cellulose, modified zein nanoparticles, a protein adhesive and a modified zein ethanol water solution. The preparation method comprises the following specific steps: reacting soybean protein isolate with cross-linking enzyme to generate a protein adhesive, reacting proanthocyanidins with gallic acid to generate gallic acylated proanthocyanidins, and carrying out multi-step treatment on zein. Experimental results show that the composition has excellent water resistance and oxidation resistance, and the growth efficiency and the survival rate of aquatic animals are remarkably improved. The preparation method is simple, the cost is low, heat-sensitive substances in the feed are not damaged, and an economical, efficient and nutrient-rich feed solution is provided for aquaculture.

The invention discloses application of a scutellaria baicalensis SbMYB8 gene in promotion of anthocyanin synthesis, the application comprises the steps that the SbMYB8 gene is constructed to a plant expression vector and introduced into plant cells to promote synthesis of plant anthocyanin, and the nucleotide sequence of the SbMYB8 gene is shown as SEQ ID NO: 1, or the amino acid sequence coded by the SbMYB8 gene is shown as SEQ ID NO: 2. The MYB transcription factor SbMYB8 gene with the function of promoting anthocyanin synthesis is cloned and identified from the scutellaria baicalensis for the first time, and the blank of a transcription regulation mechanism for regulating anthocyanin metabolism in the scutellaria baicalensis is filled; the SbMYB8 gene can significantly promote accumulation of anthocyanin in various plants, shows good heterologous expression stability and adaptability, and has wide cross-species application potential.

The invention belongs to the technical field of emulsion gel preparation, and discloses a preparation method of interpenetrating cross-linked network emulsion gel for improving the stability of anthocyanin and xanthophyll. According to the invention, a whey protein network induced by TG enzyme and a sodium alginate network induced by Ca < 2 + > are mutually crosslinked to form emulsion gel with an interpenetrating network structure, and purple sweet potato anthocyanin extract and xanthophyll are jointly loaded. The whey protein and sodium alginate interpenetrating cross-linked network emulsion gel is good in rheological property, uniform in micro-pore distribution and high in anthocyanin and xanthophyll stability, has the advantages of being natural, environmentally friendly, green and sustainable, and has wide application prospects in the field of functional food.

The invention discloses aronia melanocarpa plant composite powder and a preparation method thereof. The aronia melanocarpa plant composite powder comprises the following raw materials in parts by weight: 40-50 parts of aronia melanocarpa powder, 15-20 parts of dihydroquercetin powder, 10-15 parts of black sesame powder, 2-5 parts of honeysuckle flower powder, 1-2 parts of dandelion powder, 0.5-2 parts of radix polygonati officinalis powder and 1-3 parts of mint powder. The problems that existing aronia melanocarpa powder is single in component and prone to functional limitation, the single aronia melanocarpa powder mainly provides anthocyanin antioxidant components and has the functions of resisting oxidation, protecting eyes and resisting inflammation, but the effect dimension is single, the health requirements of consumers for comprehensive conditioning are difficult to meet, and the single aronia melanocarpa powder is obvious in acerbity and poor in taste and has a certain health care effect are solved. The aronia melanocarpa plant composite powder compounded with functional plant components is urgently needed to solve the problem that the aronia melanocarpa plant composite powder is easy to cause taste fatigue after being drunk for a long time.

The invention discloses a preparation method of a quinoa-fruit compound beverage, and belongs to the technical field of beverage processing. Comprising the following steps: S1, raw material pretreatment (in the quinoa pretreatment, high-viscosity enzymolysis modified quinoa pulp is prepared through double-enzyme synergistic hydrolysis of acid protease and natural kiwi fruit protease in kiwi fruit juice in combination with gradient heating and grinding; the preparation method comprises the following steps: pretreating raspberries, namely preparing suspended raspberry seed pulp by freeze-drying, superfine grinding and coating with enzymolysis modified quinoa pulp, and meanwhile, adding tea polyphenol, glycerol and a compound buffer agent to prepare color-protecting anti-crosslinking raspberry juice), S2, blending and homogenizing, and S3, sterilizing and filling. Through three stages of raw material pretreatment, blending and homogenization, and sterilization and filling, the nutritional conflict problems mentioned in the background technology, such as quinoa protein acid denaturation and aggregation, quinoa PPO-mediated raspberry anthocyanin oxidation browning, raspberry seed retention and suspension stability, and taste adaptation, are solved.

The application belongs to the technical field of food processing, and particularly relates to an oat protein-pectin-proanthocyanidin compound and a preparation method thereof. The oat protein is mixed with water, and an alkali liquor is added to adjust the pH to obtain a protein material solution for medium grinding. Then, pectin is added to the ground protein material solution, and proanthocyanidin powder is added after the pH is adjusted, and secondary medium grinding is performed to obtain an oat protein-pectin-proanthocyanidin solution. Then, hydrochloric acid is slowly added to adjust the pH to 6.8-7.0. Finally, the protein solution is centrifuged, and the supernatant is spray dried to obtain the oat protein-pectin-proanthocyanidin compound. The oat protein-pectin-proanthocyanidin compound prepared by the method is a brand-new protein system, and solves the problems of poor dispersibility, nutritional defects and poor emulsifying property of oat protein, and has a wide application prospect.

The invention relates to the technical field of preparation of Pickering emulsion stabilizers, in particular to a protein-polyphenol-polysaccharide ternary covalent complex as well as a preparation method and application thereof. The method comprises the following steps: step 1, preparing a whey protein isolate aqueous solution, completely hydrating the whey protein isolate aqueous solution, compounding a grape seed oligomeric proanthocyanidins aqueous solution and a hydrated protein aqueous solution, adjusting the pH value, and carrying out stirring reaction and dialysis freeze-drying to prepare a protein-polyphenol binary compound; and 2, compounding the binary compound solution with the lycium barbarum polysaccharide solution, uniformly stirring, carrying out Maillard reaction to obtain a ternary compound solution, and freeze-drying to obtain the protein-polyphenol-polysaccharide ternary compound. The invention provides a non-toxic, harmless and pollution-free natural emulsifier and stabilizer, and simultaneously prepares a Chinese wolfberry seed oil Pickering emulsion capable of loading nutritional ingredients and active substances, thereby providing reference for screening research of the natural emulsifier and protection of nutritional ingredients of Chinese wolfberry seed oil, which are easy to photodecompose and oxidize; the problem that the effect of protein serving as a Pickering emulsion stabilizer in the prior art is poor is solved.

The invention relates to the technical field of flammulina velutipes planting, in particular to a flammulina velutipes culture method and compost, which comprises the following steps: S1, preparing purple sweet potato water; the preparation method comprises the following steps: washing fresh purple sweet potatoes, mixing with water in proportion, boiling, cooling, filtering, and taking filtrate as purple sweet potato water for later use; s2, preparing a culture material; the raw materials comprise corncobs, rice bran, bran, cottonseed hulls, sugar beet pulp, soybean hulls, corn flour, brewer's grains, calcium carbonate and shellfish fossils. The preparation method comprises the following steps: weighing solid raw materials in proportion, stirring the raw materials in a stirrer for 10 minutes to obtain a mixture, uniformly mixing the mixture, adding the purple sweet potato water prepared in the step S1, fully stirring the mixture until the mixture is uniformly mixed, and controlling the water content of a final culture material to be 62-68% to obtain the culture medium. The purple sweet potato water is used for completely replacing tap water to serve as a culture material component, the purple sweet potato water is not only a solvent, but also a natural nutrient solution rich in anthocyanin, polysaccharide, vitamins and mineral substances, flammulina velutipes hyphae can absorb and convert the substances, and the anthocyanin content in sporocarp is remarkably increased.

The invention relates to the technical field of scenic spot plant growth monitoring, and particularly discloses a scenic spot plant growth intelligent monitoring method and system, and the method comprises the steps: carrying out the multi-band image data collection of ornamental vegetation, and obtaining leaf spectrum purification data; extracting wave band response curves of anthocyanin, carotenoid and chlorophyll according to the spectral purification data to obtain pigment wave band characteristic data; performing spectrum aliasing sensitivity analysis on the pigment wave band characteristic data to obtain spectrum aliasing nonlinear characteristic data; carrying out differentiation attribution calculation on a wave band aliasing region between the healthy purple leaves and the iron-deficient yellowing to obtain aliasing difference attribution data; and finally obtaining vegetation pigment unmixing mapping data. Performing physiological status difference identification on the pigment unmixing mapping data to obtain a vegetation health monitoring result; the precision, high efficiency and foresight of scenic spot vegetation monitoring are technically guaranteed, the technical requirements of modern scenic spot vegetation management are met, and losses caused by vegetation health problems are effectively reduced.

A trace element supplement consisting of lutein, zeaxanthin, astaxanthin, anthocyanins, and proanthocyanidins is designed to improve retinal health in children and adolescents, relieve eye strain, and slow the progression of myopia. The daily dose is 1–20 mg lutein, 0.2–4 mg zeaxanthin, 0.5–8 mg astaxanthin, 10–700 mg taurine, and 0.5–8.5 mg zinc; the nighttime dose is 1–100 mg eyebright extract, 10–300 mg chamomile extract, 1–200 mg anthocyanins, and 1–500 mg proanthocyanidins.

The invention discloses a high-nutrition additive-free candy jam based on seaweed dietary fibers and a preparation method of the high-nutrition additive-free candy jam. The jam comprises the following components in parts by weight: 55-70 parts of a fruit base material, 0.7-1.3 parts of sugar beet-sourced low-methoxy amidated pectin, 2.2-4.2 parts of a seaweed dietary fiber compound system, 0.06-0.18 part of calcium salt, 0.08-0.25 part of an anthocyanin stable system and the balance of water, and exogenous saccharides and artificial or natural sugar substitutes are not added. Wherein the methoxylation degree of pectin is smaller than or equal to 35%, the amidation degree of pectin is 5-20%, and the seaweed dietary fiber compound system comprises brown seaweed soluble dietary fibers and red seaweed galactan dietary fibers. The preparation method comprises the following steps: pulping at low temperature and adjusting acid, dispersing and hydrating polysaccharide, adding glue in batches, degassing and controlling oxygen in stages, introducing laccase for covalent cross-linking, adding calcium in stages to form ionic cross-linking, adding an anthocyanin stabilizing system, filling at low temperature, and carrying out 580-620MPa ultrahigh pressure treatment. A stable gel structure and good water binding capacity are obtained under the condition of no exogenous sugar, bleeding is reduced, anthocyanin and color stability are improved, and meanwhile non-thermal sterilization and storage period safety are achieved.

The invention discloses the technical field of anthocyanin extraction, and particularly relates to a method for extracting anthocyanin in blueberries by using a sodium alginate immobilized enzyme. According to the method, blueberries are used as a raw material, sodium alginate is used as a carrier to immobilize cellulase, and process conditions for extracting anthocyanin are optimized, including sodium alginate concentration, enzymolysis temperature and time, enzyme concentration and enzyme proportion. The immobilized enzyme is applied to an anthocyanin extraction process, when the temperature is 50 DEG C and the reaction time is 3h, the yield of anthocyanin is improved by 18.7% compared with that of free enzyme, and the activity of the immobilized enzyme still keeps 75% or above after the immobilized enzyme is repeatedly used for five times. In addition, the sodium alginate immobilized enzyme shows good thermal stability.

The invention discloses a formula composition which is prepared from the following components in parts by weight: 100 to 400 parts by weight of grape seed procyanidine, 100 to 300 parts by weight of pomegranate polyphenol, 10 to 100 parts by weight of ergothioneine, 50 to 200 parts by weight of catechin and 100 to 400 parts by weight of astragalus polysaccharide. According to the invention, cell-level anti-aging is taken as a core logic, five active ingredients of grape seed procyanidine, pomegranate polyphenol, ergothioneine, catechin and astragalus polysaccharide are scientifically compounded, a multi-dimensional anti-oxidation defense system is constructed, and multiple effects of scavenging free radicals, repairing cell damage, delaying an aging process and the like can be realized.

The invention relates to the technical field of functional nutritious food, in particular to multi-layer skin nutrition powder with a whole-spectrum skin growth and repair promoting effect and a preparation method and application thereof, the core of the multi-layer skin nutrition powder is composed of a collagen peptide system (60-62%), a proteoglycan system (20-22%) and the like, collagen peptide is compounded with three sources of cattle, fish and chicken, and the multi-layer skin nutrition powder has the advantages that the multi-layer skin nutrition powder is prepared; according to the present invention, with the combination of the following components, the skin structure and activity are jointly promoted by using the synergistic effect of the functional polypeptide compound (2.5-3.5%) and the plant active component (3.5-4.5%) rich in asiaticoside and procyanidine, and the nutrition enhancers (4-5%) such as L-ascorbic acid and amino acid chelated zinc and the food-grade auxiliary materials (8-10%) such as microcrystalline cellulose are integrated in the formula, such that the various systems achieve the synergistic effect so as to achieve the effect of nourishing the skin; through the synergistic compatibility of the three-source collagen peptide, the comprehensive supplement of various types of collagen in skin tissues is realized.

The invention discloses a preparation method and application of a blueberry anthocyanin extract, and belongs to the technical field of natural product extraction. The preparation method comprises the following steps: mixing blueberry pomace powder and water according to a solid-liquid ratio of 1: (10-50), exhausting, performing ultrasonic-assisted extraction, adding hemicellulase accounting for 0.6-1.4% of the mass of the pomace powder, extracting at 40-60 DEG C for 1-4 hours, and finally centrifuging to obtain a blueberry anthocyanin extracting solution. According to the method, the blueberry pomace is cooperatively treated by ultrasonic waves and hemicellulase, so that the defects of low efficiency and easy degradation of anthocyanin in a traditional extraction method are overcome, and the method has the outstanding advantages of high extraction rate, good activity maintenance and green and environment-friendly process. The obtained extract shows excellent DPPH and ABTS < + > free radical scavenging ability, and can be widely applied to functional food, health care products and cosmetics as a natural antioxidant.

The invention relates to the technical field of preparation of cured beef, and discloses low-salt cured beef which comprises main materials and auxiliary materials in parts by mass as preparation raw materials, the main materials comprise fresh yellow cattle meat and compound salt, and the auxiliary materials comprise tea polyphenol, alanine, calcium gluconate and a kelp powder aqueous extract. The formula comprises 100 parts of fresh yellow cattle meat, 5 parts of compound salt, 0.1 part of tea polyphenol, 0.2 part of alanine, 0.5 part of calcium gluconate and 1 part of kelp powder aqueous extract. The method comprises the following steps: putting cleaned beef strips into a microwave oven, mixing 5% of compound salt with tea polyphenol, anthocyanin, rosemary extract, alanine and calcium gluconate in order to improve the pickling efficiency, adding 50 parts of clear water, and uniformly stirring to prepare a pickling solution; and cooling the fried cured beef to room temperature, packaging with a vacuum packaging bag, and carrying out ultrahigh pressure treatment (300MPa, 5 minutes) to replace irradiation, prolong the shelf life and retain the flavor, so that the method has the advantages of traditional flavor, prolonged shelf life, simple production process and the like.

The present disclosure provides compositions and methods for producing condensed tannins in maize. The present disclosure further provides maize plants, seeds, cells, and plant parts comprising increased catechin based proanthocyanidins or increased condensed tannins. Aspects of the disclosure further relate to methods for producing, growing, and breeding modified maize plants.

The invention relates to the technical field of food packaging, in particular to a dual-antioxidant packaging film as well as a preparation method and application thereof. The dual antioxidant packaging film disclosed by the invention is prepared from the following components in parts by mass: 3.2 to 4 parts of starch, 0.8 to 1 part of glycerol, 0.8 to 1 part of polysaccharide, 0.02 to 0.16 part of berberine / anthocyanin self-assembled nanoparticles and 93.98 to 95.2 parts of water. The packaging film prepared by the invention is uniform in structure, has good mechanical properties and moisture and oxygen barrier properties, shows an intelligent slow release characteristic in a pH value change or oxidation environment, can realize controllable release of the tea tree essential oil, and significantly improves the antioxidant and antibacterial activity of the film. The material source is natural, renewable and biodegradable, and the environment-friendly fresh-keeping packaging material is suitable for fresh-keeping packaging of foods such as fruits, vegetables and meat products, and has good environment friendliness and industrial application prospects.

The application discloses a pH-responsive degradable composite bacteriostatic film derived from waste bone powder extract and a preparation method thereof. The main components of the film are collagen and hydroxyapatite extracted from waste bone powder, citric acid monohydrate-betaine and lactic acid-betaine eutectic solvent, and in addition, purple corn anthocyanin is used as a pH response agent, and citric acid monohydrate-betaine eutectic solvent is used as a crosslinking agent and a bacteriostatic agent. In the preparation process, collagen and hydroxyapatite are mixed with an aqueous solution of eutectic solvent and heated, and after the system is reduced to room temperature, the anthocyanin aqueous solution is added, mixed, and ultrasonic defoaming is performed, then the film forming liquid is poured into a clean mold, vacuum dried, and cooled to remove the film. The pH-responsive degradable composite film prepared based on the above method has good properties, not only has the characteristics that the overall color changes with the microenvironment pH, but also has good mechanical strength, bacteriostasis and degradability, and has wide application potential in many fields such as food preservation.

The invention relates to a process for purifying maritime pine bark by selective sieving and grinding, as well as the extracts obtained from this purified bark. The purified bark of the invention is obtained by a three-step process consisting of pre-sieving the raw bark, followed by selective grinding of the pre-sieved bark with a non-shearing grinder, followed by sieving of the selectively ground raw bark. The maritime pine bark extracts obtained from this purified bark can be obtained by extraction with water or with a mixture of water and ethanol. Extracts obtained by water extraction have a procyanidin content of between 65% and 75%, while extracts obtained with a mixture of water and ethanol have a procyanidin content greater than or equal to 70%.Extracts of maritime pine bark obtained from the purified bark of the invention can be used as ingredients for the formulation of food supplements, functional foods or cosmetic products.

The present application relates to anthocyanin extraction technical field, disclose a kind of berry anthocyanin extraction equipment and extraction method, extraction equipment includes: extraction machine, the working area of extraction machine is provided with fruit juice cold filtering mechanism, for cold treatment berry and realize efficient extraction;Fruit juice cold filtering mechanism includes four vertical setting mid vertical tube, cloth bag and supply suction liquid component;Wherein: four mid vertical tubes are fixed in matrix in the center position of extraction machine working area, form stable support structure;Mid vertical tube top and limiting disc form rigid connection, bottom is fixedly arranged bottom disc by welding mode;Supply suction liquid component is connected with four mid vertical tubes;Limiting disc is rotatably provided with bevel gear disc outside by bearing assembly, form rotary drive structure;The present application is by setting fruit juice cold filtering mechanism, using 0 to 5 ℃ low temperature extraction liquid and pure water form high pressure cold vortex flow, while physically crushing berry, effectively avoid the damage of high temperature to anthocyanin activity.