1348 results about "Anthocyanidins" patented technology

The invention discloses noodles with a blood sugar control function, which comprise the following components in parts by weight: 150-170 parts of triticale flour, 10-30 parts of cyperus esculentus powder, table salt and water, and 4-10 parts of dietary fibers, 0.5 to 1 part of anthocyanin; 0.03 to 0.12 part of mineral substances and 0.2 to 0.5 part of vitamin B. The traditional Chinese medicine composition has the effects of improving digestion and metabolism, delaying senescence, strengthening body functions, assisting in controlling blood sugar, enhancing satiety and assisting weight management.

The invention provides a technical method for improving the anti-aging effect of eurotium cristatum tea compound fermentation liquor, and belongs to the technical field of microorganisms, the Latin name of eurotium cristatum is Eurotium cristatum, the eurotium cristatum is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 2024643. According to the method, the yeast seed solution is inoculated into the tea-sea buckthorn leaf culture solution fermented by the eurotium cristatum, so that the content of anti-aging active components such as procyanidine B2, quercitrin, cyanidin-3-glucoside, nicotinic acid, naringenin and rutin in the fermentation culture solution can be remarkably increased through co-fermentation, and the elastase inhibition rate can be remarkably increased; the anti-aging effect of a component system of the fermentation culture solution is enhanced. The prepared tea-hippophae rhamnoides leaf compound fermentation liquor is suitable for development of anti-aging cosmetics and has a wide market prospect.

The invention belongs to the technical field of sweet potato breeding, and provides an SNP marker related to sweet potato root color and anthocyanin content and application of the SNP marker, the SNP marker is located at the 25658800th site of a chromosome 10a of sweet potatoes, and the base polymorphism is A / T. When the SNP genotype of the sample to be detected is A / T, the content of anthocyanin in the tuberous roots is high, and the color is purple; when the SNP genotype is T / T, the content of anthocyanin in the tuberous roots is low, and the tuberous roots are non-purple in a large probability. According to the detection method and the kit provided by the invention, the efficiency of identifying the purple sweet potatoes by the developed SNP marker through sweet potato natural population phenotype identification and verification reaches 100%, the efficiency of identifying the non-purple sweet potatoes reaches 85.04%, the color of the sweet potato tuberous roots and the anthocyanin content can be rapidly and accurately detected, and the detection method and the kit have the advantages of being simple and convenient to operate, low in cost and the like, and have wide application prospects. The requirement of large-scale molecular marker-assisted selection can be met.

The invention discloses a beta-glucuronidase inhibitor composition as well as a preparation method and application thereof. The composition is prepared from the following substances: ursolic acid, procyanidine, icariin, curcumin, epigallocatechin gallate and glycyrrhetinic acid. The beta-glucuronidase inhibitor composition has a broad-spectrum and good inhibition effect, the preparation method is simple, large-scale production is easy, and the inhibitor with a natural source is higher in use compliance, lower in toxic and side effects and more suitable for developing intestinal health products and has an application prospect.

The invention relates to the technical field of high-barrier environment-friendly packaging films, and discloses a high-barrier environment-friendly packaging film with an antibacterial function, the high-barrier environment-friendly packaging film comprises a base layer, a nano composite barrier layer, a bio-based functional layer and an outer protective layer from bottom to top in sequence, and the layers are tightly attached through a coated starch-based adhesive. According to the high-barrier environment-friendly packaging film with the antibacterial function, good biodegradability of polyhydroxyalkanoate adopted in the base layer film, polycaprolactone which is adopted in the nano-composite barrier layer film and has a biodegradable function and konjac glucomannan in the biological functional layer film are utilized; the composite packaging film can be decomposed into carbon dioxide and water by microorganisms in various environments, has excellent flexibility and processability, can adapt to different packaging requirements, provides basic flexibility, structural support and green and environment-friendly effects for the whole packaging film, and has a wide application prospect. The procyanidine arranged in the outer protective layer film provides stronger antibacterial property and water solubility for the whole packaging film.

The invention relates to a natural antioxidant for prefabricated vegetables and a preparation method of the natural antioxidant, and belongs to the technical field of food additives. The antioxidant is prepared from rosmarinic acid, carnosic acid, drug-loaded cellulose and double-phase chelating microspheres; the drug-loaded cellulose is prepared by modifying cellulose with quercetin, loading anthocyanin and then wrapping with a trehalose-gamma-polyglutamic acid composite film; the biphase chelating microspheres are phytic acid-citric acid biphase sustained-release microspheres formed by wrapping phytic acid and citric acid with a chitosan and sodium alginate composite film. Through multi-component functional complementation, multi-stage slow release design and physical-chemical synergistic barrier, comprehensive inhibition of the oxidation problem of the prefabricated vegetable is realized, a'free radical scavenging-metal chelating-phase interface stabilization 'triple protection system is constructed, accurate oxidation resistance in different stages of processing, storage and transportation is realized, and the prepared vegetable has good market prospects. The bottlenecks of short action period and single protection dimension of a natural antioxidant are broken through.

The invention belongs to the field of medical instrument coloring agents, and provides a natural anthocyanin endoscope coloring agent. According to the invention, the innovative design of combining a ternary color-stabilizing compound formed by anthocyanin, a co-chromophore and hydroxypropyl beta cyclodextrin with a chitosan sodium alginate polysaccharide system and a composite buffer system is adopted, and the viscosity is controlled to be 200-320 millisecond, the particle size of spray droplets is 40-60 microns, and the thickness of a thin film is 10-30 microns through specific molar ratio compatibility and a genipin crosslinking technology. Through accurate regulation and control, the excellent performance that a double-network thin layer is formed under the calcium ion environment that the absorbance retention rate at 520 nanometers is not lower than 90% and the elution rate is not lower than 90% under the irradiation of hundred thousand lux white light is achieved, the technical problems that a traditional endoscope coloring agent is poor in safety, low in stability and insufficient in mucous membrane contrast ratio are solved, and the wide clinical application value is achieved.

The invention belongs to the technical field of plant biology, and discloses application of FvNAC073 in regulation and control of strawberry fruit ripening or fruit size. The invention finds that FvNAC073 has an important biological function in strawberry ripening or fruit size, after the FvNAC073 is knocked out, the anthocyanin content in strawberry fruits is reduced, the fruit softening is delayed, the abscisic acid content in the fruits is reduced, the longitudinal and transverse diameters of the fruits are reduced, and the weight of a single fruit is reduced; after the FvNAC073 is over-expressed, the content of anthocyanin in strawberry fruits is increased, the fruits are softened in advance, the content of abscisic acid in the fruits is increased, the longitudinal and transverse diameters of the fruits are increased, and the weight of a single fruit is increased, so that a new gene resource can be provided for molecular breeding for researching the sizes of the strawberry fruits and ripening of the strawberries.

The invention relates to the technical field of pet food antioxidants, in particular to a compound pet food antioxidant containing plant polyphenol compounds and a preparation method of the compound pet food antioxidant. 10 to 20 parts of a grape seed extract; 8 to 15 parts of a rosemary extract; 5 to 10 parts of L-ascorbyl palmitate; 5 to 10 parts of alpha-tocopheryl acetate; 3-8 parts of citric acid; 8 to 15 parts of soybean lecithin; 20 to 30 parts of medium chain triglyceride; eGCG (epigallocatechin gallate) in the green tea contains a plurality of o-dihydroxyl structures, the grape seed procyanidine has a unique C6-C3-C6 skeleton, and carnosic acid in the rosemary belongs to a diterpenoid compound. Due to the diversity of the structure, the compound can play a role in different types of free radicals and oxidation reactions, and a broad-spectrum anti-oxidation network is formed.

The invention relates to the technical field of food packaging, and discloses a composite preservative film as well as a preparation method and application thereof. The composite preservative film comprises a pectin film layer and a chitosan film layer which are arranged up and down, the pectin film layer contains an additive, the chitosan film layer contains lignin, and the additive is anthocyanin and / or an anthocyanin silver ion compound. The method comprises the following steps that S1, under the condition that a solvent exists, pectin and an additive are mixed I, then a pectin film layer is prepared through a tape casting method, and the additive is anthocyanin and / or an anthocyanin silver ion compound; in the presence of a solvent, chitosan and lignin are mixed II, and a chitosan film layer is prepared through a tape casting method; and S2, compounding the pectin film layer and the chitosan film layer to prepare the composite preservative film. The composite preservative film has antibacterial activity on various food spoilage bacteria and is high in oxidation resistance.

The invention discloses an anthocyanin content detection method, system and device and a medium, and relates to the field of agricultural product detection.The anthocyanin content detection method includes the steps that by setting an initial learning rate, using a regularization strategy to optimize weight attenuation, setting a threshold value of a tolerance value and adding a batch normalization and random inactivation module, a CNN model is prevented from being over-fitted; in a convolutional neural network model CNN for preventing overfitting, a residual block and an attention mechanism Mangban MANBa based on multi-head attention and normalization are added for optimization, and an activation function in the optimized convolutional neural network model CNN is replaced with a self-regular non-monotonic neural activation function Mish to obtain an adjustment model; and training and adjusting the model by using the characteristic spectrum variable of the anthocyanin so as to obtain the anthocyanin prediction model capable of predicting the anthocyanin content. According to the invention, an accurate component content result can be detected.

1. A composition, which is an anthocyanin-based colorant composition comprising 50-90 mol-%, based on the total amount of anthocyanins, of pelargonidin-based anthocyanins, and wherein (i) >70 mol-% of all anthocyanins are acylated with at least one phenolic acid; and (ii) >20 mol-% of all anthocyanins are acylated with at least one hydroxycinnamic acid; and wherein the composition has a red color with a hue value H≥ in the L*C*h* color system in the range of 10-30, measured at an L*-value of (70.0±0.1) in a 0.1 mol / l trisodium citrate dihydrate buffer at pH 3 in a 1 cm-length quartz cell using Spectraflash 650 (Datacolor) in transmission mode under D65 illuminant 10 Deg. Also, the present invention relates to use of the above compositions as a food colorant.

The present invention discloses a method and application of preparing a freshness indicator label by electrostatic spinning, which is obtained by the following steps: S1: adding bayberry anthocyanin to a matrix solution to obtain a spinning solution; S2: after ultrasonic filtration of the matrix solution, taking part of the solution and putting it into a 10ml syringe, fixing it on a propulsion pump, spinning using a high-voltage electrostatic spinning device under a specific voltage, receiving distance, flow rate, temperature and humidity to prepare a nanofiber membrane; S3: balancing the prepared nanofiber membrane for 48 hours, and cutting it into a specific size to prepare a freshness indicator label. The freshness indicator label prepared by the method of the present invention has a large specific surface area and porosity, and is sensitive to pH response. The bayberry anthocyanin freshness indicator label based on pullulan / polyvinyl alcohol has great application potential in the freshness monitoring and intelligent packaging of white shrimp.

The invention relates to application of procyanidine in preparation of a medicine for inhibiting and killing in-vitro parasitic ciliates of fishes, and belongs to the technical field of medicines for fish parasitic diseases. The procyanidine is applied to preparation of drugs for inhibiting and killing in-vitro parasitic ciliates of fishes. The procyanidine is applied to preparation of the medicine for inhibiting and killing the in-vitro parasitic ciliates of the marine fishes for the first time, especially for inhibiting and killing cryptocaryon irritans, the in-vitro parasites can be completely inhibited and killed within an effective dosage range, and the effect of preventing and treating the ichthyophthiriasis of the marine fishes is achieved. The procyanidine serving as a main medicine component is wide in source, has an industrial preparation process, is low in price, has a huge potential of being developed into a novel fish medicine for preventing and treating in-vitro parasitic ciliates of fishes, and is wide in application prospect. The procyanidine has small toxic and side effects, is easy to degrade and eliminate, can effectively avoid negative effects such as environmental pollution and drug residues, and promotes sustainable, green and healthy development of the aquaculture industry.

The invention relates to the technical field of toothpaste preparation, in particular to hydrolyzed procyanidine-containing Chinese herbal medicine nursing toothpaste as well as a preparation method and application thereof.The preparation method of the hydrolyzed procyanidine-containing Chinese herbal medicine nursing toothpaste comprises the following preparation steps: adding a humectant, a thickening agent, a surfactant, a sweetening agent and a preservative into deionized water, and uniformly stirring; uniformly stirring to obtain a mixed solution; adding the abrasive into the mixed solution, and uniformly stirring to obtain paste; and adding the active microcapsules into the paste, uniformly stirring, carrying out vacuum degassing, and filling to obtain the Chinese herbal medicine nursing toothpaste containing hydrolyzed procyanidine. The Chinese herbal medicine care toothpaste is fused with hydrolyzed procyanidine, and shows excellent oral care performance. The oral care solution has obvious inhibition capability on a plurality of common oral pathogenic bacteria, has extremely high bacteriostasis rate, and can effectively block bacterial breeding and maintain oral health from the source. Meanwhile, the toothpaste is excellent in deodorization effect and capable of rapidly decomposing oral cavity peculiar smell molecules and remarkably improving oral cavity freshness.

The invention discloses a mint McTTG2 gene as well as an expression protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the mint McTTG2 gene disclosed by the invention is shown as SEQ ID NO. 1, and the amino acid sequence of the expression protein of the mint McTTG2 gene is shown as SEQ ID NO. 2. The method comprises the following steps: constructing an expression vector of a mint McTTG2 gene, and transforming the expression vector into an arabidopsis thaliana mutant; and cultivating and screening to obtain a transgenic arabidopsis thaliana plant of which the procyanidine content is obviously increased. A transgenic experiment shows that after the McTTG2 gene is over-expressed in the mutant ttg2-7, the content of procyanidine is remarkably increased; compared with the mutant ttg2-7, the number of epidermal hairs on leaves of ttg2-7 / 35S:: McTTG2-1 and-2 strains is obviously increased, which is close to that of wild strains. Gene resources are provided for plant variety improvement, and cultivation of new mint varieties is facilitated.

The invention relates to the field of cosmetics, in particular to a berry composition for scalp care and a preparation method and application thereof, and the preparation method comprises the following steps: A, pretreating fruits to obtain berry freeze-dried powder; b, preparing a deep eutectic solvent; c, mixing the berry freeze-dried powder with the deep-eutectic solvent, and performing oscillation extraction to obtain a berry procyanidine crude extract; d, purifying the berry procyanidine crude extract with macroporous resin to obtain a first purified berry procyanidine product; e, removing the solvent from the first purified berry proanthocyanidins to obtain a second purified berry proanthocyanidins; and F, dissolving epicatechin, mixing the dissolved epicatechin with the second purified product of the berry procyanidine, uniformly heating in a vacuum environment, cooling after relieving the vacuum environment, mixing with amino acid hydrochloride, and preserving heat to obtain the berry extract.

The invention relates to the technical field of biological medicine, and particularly provides visual hydrogel based on multiple response mechanisms as well as a preparation method and application of the visual hydrogel. The preparation method comprises the following steps: (1) preparing an inclusion compound from beta-cyclodextrin, hydroxypropyl-beta-cyclodextrin and anthocyanin; (2) respectively preparing a polyvinyl alcohol / rhein solution and a mixed solution of quaternary ammonium salt chitosan / hyaluronic acid / kappa-carrageenan; and (3) initiating dynamic crosslinking through phenylboronic acid, constructing a multi-network structure by cooperating with tannic acid, the clathrate compound and genipin, and performing gradient temperature control curing to form the hydrogel. The hydrogel has the pH / temperature / ROS response characteristic, the wound surface microenvironment state can be fed back in real time by means of anthocyanin color change, meanwhile, hyaluronic acid / polyvinyl alcohol can construct a 3D microchannel structure, ordered arrangement of collagen is promoted, wound surface healing is accelerated, and the wound surface healing effect is improved. Therefore, the problems that direct loading of anthocyanin is unstable, and a single response mechanism is difficult to adapt to a complicated microenvironment of a diabetic wound surface are solved.

The invention discloses a mint McTTG1 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the mint McTTG1 gene disclosed by the invention is shown as SEQ ID NO.1, the amino acid sequence of the expression protein of the mint McTTG1 gene is shown as SEQ ID NO.2, and the mint McTTG1 gene belongs to WD40 family genes participating in regulation and control of epidermal hair development and synthesis of anthocyanin and procyanidine. Transgenic experiments show that after the McTTG1 gene is over-expressed in the arabidopsis thaliana mutant ttg1-13 with epidermal hair loss and significantly reduced accumulation of anthocyanin and procyanidine, the formation of the epidermal hair on the surface of the mutant can be recovered, and the accumulation of anthocyanin and procyanidine can be promoted. Gene resources are provided for plant variety improvement, and cultivation of new mint varieties is facilitated.

The invention belongs to the technical field of extraction and purification of plant components, and relates to a low-temperature freezing vacuum drying nano processing method for extracting anthocyanin from purple corn, in particular to a method for extracting anthocyanin from corn kernels, cores, straws, roots and stigmas of purple corn. The processing method comprises the steps of pretreatment, concentration, hydroxyl radical treatment, quick freezing, nano crushing and the like. A hydroxyl radical treatment method is adopted, so that harmful substances such as pesticide residues can be efficiently, quickly and thoroughly removed; the effect of extracting the anthocyanin from corn kernels, roots, cores, stems and stigma of purple corn by adopting a low-temperature freezing vacuum drying technology is remarkable, the purification rate is high, and the anthocyanin containing 99% of biological activity is achieved. The nanoscale anthocyanin is processed by adopting jet mill equipment and can be conveniently absorbed by a human body and widely applied to various fields.

The invention belongs to the technical field of gene engineering, and provides a gene OsMYB1 and application thereof in negative regulation of anthocyanin synthesis. The cDNA (complementary deoxyribonucleic acid) sequence of the gene OsMYB1 is as shown in SEQ ID NO. 1. A systematic molecular biology experiment proves that the OsMYB1 inhibits the expression of the OsDFR by combining with a promoter region of an anthocyanin synthesis key structural gene OsDFR, so that the synthesis of anthocyanin is inhibited. Meanwhile, the OsMYB1 can form an MBW ternary complex with the OsB2 and the OsPAC1 to competitively inhibit the synthesis of the anthocyanin, which indicates that the transcription factor OsMYB1 is a negative regulation factor for the synthesis of the rice anthocyanin, the content of the rice anthocyanin can be increased by knocking out the transcription factor OsMYB1, and a certain theoretical basis is provided for cultivating high-quality purple rice with high anthocyanin content.

The invention belongs to the field of nano-medicine and traditional Chinese medicine, and particularly discloses an antioxidant nano-material based on self-assembly of traditional Chinese medicine and copper ions and a preparation method thereof.The nano-material is prepared from BPCu nano-particles formed by self-assembly of berberine and procyanidine B2 under induction of the copper ions, the BPCu nano-particles are further subjected to surface modification through polyvinylpyrrolidone, and the antioxidant nano-material is prepared. And the stably dispersed PVP-coated BPCu nano structure is obtained. The material is small in particle size, uniform in distribution, good in water solubility and storage stability, excellent in free radical scavenging capacity and catalase-like activity and suitable for adjuvant therapy of oxidative stress related diseases, and the preparation method is easy and convenient to operate, does not need an organic solvent and has good industrialization and disease treatment potential.

The invention discloses application of a plant lactobacillus CCFM1357 to conversion of anthocyanin to generate various active metabolites, inhibition of a cGAS-STING pathway and enhancement of improvement of brain aging, and belongs to the technical field of microorganisms and the technical field of medicines. The phytobacterium plantarum CCFM1357 fermented anthocyanin and the compound preparation thereof provided by the invention have the following effects: (1) a marker for relieving D-gal induced mouse brain senescence and a marker for relieving D-gal induced mouse brain senescence; (2) promoting and relieving D-gal induced mouse histopathologic characterization; (3) relieving D-gal induced mouse neuroinflammation; (4) relieving behavioral characterization of the D-gal induced aging mouse; and (5) a cGAS-STING signal channel is inhibited. The phytobacterium plantarum CCFM1357 fermented anthocyanin and the compound preparation thereof provided by the invention can be used for a product for relieving brain aging, and have a huge application prospect.

The invention provides a composition which comprises two components, and the component A comprises lutein; and the component B comprises a berry fruit extract containing anthocyanin. The composition has the effects of eliminating free radicals, resisting oxidation, protecting eyes, inhibiting tear secretion reduction, relieving eyestrain and eye dryness, relieving ciliary muscle tension, repairing retina, preventing and controlling ocular axes, protecting eyesight, preventing myopia, improving eye microcirculation, improving couperose skin and the like.

The invention belongs to the technical field of microorganisms, and relates to a plant lactobacillus NXU-F1 strain and application thereof. The classification name of the bacterial strain is lactobacillus plantarum, the bacterial strain is preserved in Guangdong Microbial Culture Collection Center on June 20, 2025, and the preservation number of the bacterial strain is GDMCC No.66563. The invention also discloses a preparation method of the bacterial strain. The plant lactobacillus is strong in growth capability, has good adaptive capacity to environment, and can obviously increase the content of antioxidant substances when being applied to plant matrix fermentation. The lycium barbarum and grape compound fermented beverage is prepared by fermenting the lycium barbarum and grape compound fermented beverage, the content of polyphenol, flavone, polysaccharide and procyanidine in the beverage is obviously increased, and the increase of resveratrol and ferulic acid is particularly obvious; meanwhile, ester compounds are increased and aldehyde substances are reduced, so that the beverage is endowed with strong longan fruity flavor, and the flavor of harmonious sour and sweet taste and lingering aftertaste is formed.

Cat litter, comprising the following raw materials in parts by weight: 40-60 parts of a bamboo extract, 10-20 parts of bean dregs powder, 5-10 parts of sweet potato dregs powder, 4-8 parts of pine sawdust, 30-50 parts of bentonite, 5-8 parts of diatomite, 5-8 parts of volcanic rock particles, 4-6 parts of perlite powder, 0.5-1.5 parts of anthocyanin, and 4-6 parts of a binder. Further provided is a preparation method for cat litter, comprising the following steps: S1, preparing raw materials; S2, preliminarily mixing the raw materials; S3, grinding; S4, preparing a liquid; S5, performing secondary mixing; S6, performing granulation; and S7, drying. The bamboo extract is added, so that the raw materials are green and healthy, improving the safety of use, and the bean dregs powder, the sweet potato dregs powder and the pine sawdust are added, so that the material costs can be reduced, and the softness can be increased; and the cat litter has high water absorption, can be directly buried in the ground when discarded so as to serve as a soil nutrient, is environmentally friendly, degradable, safe and pollution-free, and has sustainable raw materials, thereby increasing the resource utilization rate.

The invention relates to the technical field of crop cultivation, and particularly discloses a method for increasing the anthocyanin content of triticale grains, which comprises the following steps: respectively spraying 80-100 times of leaf fertilizer diluent once at the elongation stage and filling stage of triticale growth, and spraying 6-10kg / mu each time; the formula of the foliar fertilizer is as follows: 100 mg to 150 mg of mannose, 100 mg to 150 mg of fructose, 130 mg to 160 mg of mannitol, 160 mg to 200 mg of betaine, 180 mg to 200 mg of alginic acid, 180 mg to 200 mg of fucoidin, 300 mg to 350 mg of amino acid and 1000 g of water. According to the method, the content of anthocyanin in triticale grains can be remarkably increased by spraying 80-100 times of foliar fertilizer diluent prepared by the method in the elongation stage and the pustulation stage of the triticale.

The invention relates to the field of aquatic feed, and provides a low-temperature adhesive type aquatic feed particle composition and a preparation method thereof. The composition is composed of puffed soybean meal, flour, fish meal, low-temperature directed enzyme, microcrystalline cellulose, modified zein nanoparticles, a protein adhesive and a modified zein ethanol water solution. The preparation method comprises the following specific steps: reacting soybean protein isolate with cross-linking enzyme to generate a protein adhesive, reacting proanthocyanidins with gallic acid to generate gallic acylated proanthocyanidins, and carrying out multi-step treatment on zein. Experimental results show that the composition has excellent water resistance and oxidation resistance, and the growth efficiency and the survival rate of aquatic animals are remarkably improved. The preparation method is simple, the cost is low, heat-sensitive substances in the feed are not damaged, and an economical, efficient and nutrient-rich feed solution is provided for aquaculture.

The invention provides an application of a PavMYB1 gene in regulation and control of sweet cherry anthocyanin synthesis. The nucleotide sequence of the PavMYB1 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the encoded protein of the PavMYB1 gene is as shown in SEQ ID NO. 2. The invention provides an overexpression vector and a VIGS gene silencing vector constructed by related genes, a construction method of the overexpression vector and the VIGS gene silencing vector, and a recombinant microorganism containing the vectors. Experiments prove that overexpression of PavMYB1 can increase fruit pigment accumulation and anthocyanin synthesis key gene expression, technical support and theoretical basis are provided for improvement of sweet cherry fruit quality, and revealing of a regulation mechanism of sweet cherry anthocyanin synthesis is facilitated.

The invention discloses a preparation method of a chitosan oligosaccharide-anthocyanin nanocomposite. The preparation method comprises the following steps: dissolving purple corn anthocyanin in distilled water; dissolving chitosan oligosaccharide in distilled water; the method comprises the following steps: adding an organic solvent into a reaction kettle, reacting at room temperature in a dark place for 4-48 hours, transferring a mixed solution into a centrifugal tube, centrifuging for 10-30 minutes to separate out nano-composite precipitates and uncombined supernatant, and then adding ultrapure water for repeated centrifugation. And finally, carrying out freeze drying treatment on the precipitate to obtain the final chitosan oligosaccharide-anthocyanin nano-composite. The nano-composite prepared by the method has high stability, good dispersibility and enhanced biological activity, and provides a new way for wide application of chitosan oligosaccharide and anthocyanin in the fields of food, cosmetics, biological medicine and the like.