Method for preparing oligomeric proanthocyanidins

A technology of proanthocyanidins and oligomers, applied in the direction of organic chemistry, can solve problems affecting product performance, etc., and achieve the effects of improved solubility, best extraction effect, and reduced content

Inactive Publication Date: 2014-07-16
PERFECT CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention is that the oligomer proanthocyanidins produced in the prior art contain a large amount of monomers and hi...

Method used

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  • Method for preparing oligomeric proanthocyanidins
  • Method for preparing oligomeric proanthocyanidins

Examples

Experimental program
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Embodiment 1

[0034] The oligomer proanthocyanidins described in this embodiment are prepared by the following method:

[0035] (1) Freeze-dry 100 g of fresh grape seeds at -15°C to -5°C and 1 to 10 Pa, then crush them and pass through a 50-mesh sieve to obtain dry powder;

[0036] (2) Take 700ml of ethanol aqueous solution with a volume concentration of 70%, extract the grape seed dry powder at 50°C, and control the pH at 4.0, extract 3 times for 30 minutes each time, combine the extracts and rough filter to obtain the crude proanthocyanidin extract ;

[0037] (3) Concentrate the above extract under reduced pressure at a vacuum degree of 0.09Mpa and a temperature of 40-50°C to a specific gravity of 1.10, and perform adsorption treatment on the obtained concentrated solution on AB-8 macroporous resin for 4 hours; and wash it away with pure water impurities;

[0038] (4) Elute the resin with 5% ethanol-water solution 2BV, collect the eluate to obtain monomeric proanthocyanidins, and discar...

Embodiment 2

[0043] The oligomer proanthocyanidins described in this embodiment are prepared by the following method:

[0044] (1) Freeze-dry 100g of fresh grape seeds at -15°C to -5°C and 1 to 10 Pa, then pulverize them, and pass through a 80-mesh sieve to obtain dry powder;

[0045](2) Take 600ml of ethanol aqueous solution with a volume concentration of 70%, extract the dry grape seed powder at 45°C, and control the pH at about 4.3, extract 3 times for 40 minutes each time, combine the extracts and rough filter to obtain the crude proanthocyanidins. liquid;

[0046] (3) Concentrate the above extract under reduced pressure at a vacuum degree of 0.09Mpa and a temperature of 40-50°C to a specific gravity of 1.15, and apply the AB-8 macroporous resin to the obtained concentrate for 3 hours; and wash away impurities with pure water ;

[0047] (4) Elute the resin with 2BV of ethanol aqueous solution with a volume concentration of 10%, collect the eluate to obtain monomeric proanthocyanidins...

Embodiment 3

[0052] The oligomer proanthocyanidins described in this embodiment are prepared by the following method:

[0053] (1) Freeze-dry 100g of fresh grape seeds at -15°C to -5°C and 1 to 10 Pa, then pulverize them, and pass through a 140-mesh sieve to obtain dry powder;

[0054] (2) Take 650ml of ethanol aqueous solution with a volume concentration of 30%, extract the dry grape seed powder at 40°C, and control the pH at 4.4, extract 3 times for 40 minutes each time, combine the extracts and roughly filter to obtain the crude proanthocyanidin extract ;

[0055] (3) Concentrate under reduced pressure to a specific gravity of 1.15 at a vacuum degree of 0.09Mpa and a temperature of 40-50°C, and perform adsorption treatment on AB-8 macroporous resin on the obtained concentrated solution for 3 hours; and wash away impurities with pure water;

[0056] (4) Elute the resin with 2BV of ethanol aqueous solution with a volume concentration of 8%, collect the eluate to obtain monomeric proantho...

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Abstract

The invention belongs to the technical field of chemical effective component extraction, and particularly relates to a method for preparing oligomeric proanthocyanidins with uniform polymerization. According to the method, 60-70% ethanol is adopted in extraction, and the pH in the extracting process is controlled to be 4.0-4.4, so that monomer, oligomeric proanthocyanidins and polymer proanthocyanidins can be effectively extracted; macroporous resin is used for adsorbing, and elution is performed according to the principle that proanthocyanidins with different polymerization degrees have different soluble properties in ethanol with different concentrations, so that oligomer products with polymerization degree truly concentrated in 2-4 can be further obtained, and the content of the effective component oligomer in proanthocyanidins can be increased.

Description

technical field [0001] The invention belongs to the technical field of extraction of chemical active ingredients, and in particular relates to a method for preparing oligomeric proanthocyanidins with uniform polymerization degree. Background technique [0002] Proanthocyanidins are polyphenolic compounds of a class of flavanol monomers and their polymers. Their common feature is that they can produce anthocyanidins when heated in an acidic medium, so they are also called proanthocyanidins. Pigment components widely exist in various plants. [0003] Grape seed procyanidine is a kind of natural plant active substances existing in grape seeds. It has good anti-oxidation and free radical scavenging effects, and has many applications in food, health products, pharmaceuticals and other fields. The basic structure of grape seed proanthocyanidins is a polymer formed by the polymerization of different numbers of catechin or epicatechin monomers. Usually, those containing 2-4 monomer...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 胡瑞连段朝辉夏颖李子安
Owner PERFECT CHINA
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