480 results about "Betacyanins" patented technology

The invention relates to lycium ruthenicum tablets and a production method thereof. The lycium ruthenicum tablets are prepared by taking lycium ruthenicum as a raw material, selecting, washing, extracting, centrifuging for separation, performing membrane concentration, performing vacuum concentration, weighing, drying, grinding, mixing, pelletizing, drying, straightening and tabletting. The purpose of the invention is to solve the problems of high procyanidine loss and high organic solvent residue content when the lycium ruthenicum tablets are produced by a traditional method. The production method is simple and low in cost, the extraction rate of procyanidine reaches more than 92%, and the prepared lycium ruthenicum tablets are convenient to carry, high in added value and free from organic solvent residues.

The invention relates to an industrial application technology of lycium ruthenicum fruit. The fresh or dry lycium ruthenicum fruit is sorted, wherein the raw materials with color and weight reaching the standard are prepared into oral capsules or fresh juice, and the disqualified raw materials are prepared into condensed juice, brewed drinks or oral chewable tablets. The fresh lycium ruthenicum fruit which contains more than 3.5% of total flavonoids and more than 3.0% of procyanidine is the qualified lycium ruthenicum fruit. The produced industrial product has the efficacies of protecting the liver, keeping the cardiac muscle healthy, supplementing energy, nourishing brain, enhancing the immunity of the human body and delaying ageing, has the effects of reducing cholesterol in the body, reducing blood fat, preventing and treating cardiovascular and cerebrovascular diseases, cancer and diabetes mellitus, and also has the efficacy of promoting digestion, inducing diuresis, relieving constipation, activating collaterals and relieving pain. The produced industrial product consumes the least energy, keeps the strong wild natural flavor of the lycium ruthenicum fruit, has remarkable functions of resisting oxidation and fatigue and can serve as an important healthcare food and a nutritional food additive.

The invention relates to a technology for extracting effective components from a natural product and in particular relates to a method for extracting oil and procyanidin from grape seeds. The method comprises the following process steps: preparing grape seeds, grinding, performing ultrasonic extraction to obtain grape seed oil, and performing ultrasonic extraction to obtain procyanidin. The method integrates the characteristics of an ultrasonic extraction method and a double-solvent extraction method, has the characteristics of low solvent dosage, low extraction cost, high extraction efficiency, less influence on the environment and the like, and can be used for effectively avoiding the problem that in the extraction process of the prior art, the bioactivities of grape seed oil and procyanidin are damaged.

The invention relates to a lycium ruthenicum Murr proanthocyanidin product. The product is a pale purple pink powder product which is prepared from fresh lycium ruthenicum Murr as raw materials and contains 40% to 70% of oligomeric proanthocyanidins, 10% to 35% of high polymer procyanidins, and 10% to 40% of other impurities after being separated and purified. The invention also discloses a preparation method of the product. The invention adopts the membrane separation technique, has simple and quick method, not only avoids using toxicant organic reagent and acquires the product having stable and uniform property, but also efficiently lowers the producing cost and can adopt the raw materials of health care product which has an antioxidant function.

The invention described herein encompasses methods and compositions of preventing or treating cancer comprising the administration of a combination of catechins and proanthocyanadins. Compositions of catechins include but not limited to, epigallocatechin gallate (EGCg), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC). Compositions of proantho-cyanains include, but are not limited to, blue fruit extracts. The unique compositions of the invention contain various combinations of the catechins and proanthocyanadins, in combination with each other or other therapeutic agents and are used to treat primary and metastatic cancers in humans. The invention also encompasses various modes of administration of the therapeutic compounds, including formulations which may be used as a dietary or nutritional supplement or as a therapeutic compound.

The invention discloses a preparation method of polyphenol of chestnut shells. The preparation method comprises the following steps of digestion, filtration and condensation, enrichment/separation, condensation and drying to obtain the polyphenol of brownish red chestnut shells. The preparation method has the advantages of high content and good quality of polyphenol with the content of oligomeric proantho cyanidins reaching more than 50 percent, simple production technology, low cost and no pollution, and is applicable to commercial production. The preparation method maximizes the utilization of the waste chestnut shell which accounts for 20 percent of a chestnut, thus enhancing the added value of chestnuts and simultaneously creating a new resource of antioxidant of polyphenol from natural plants for the food and pharmaceuticals industries.

The invention discloses a method for continuously extracting grape seed oil and proanthocyanidin from grape seeds. The method comprises the following steps of: (1) treating grape seeds, and performing crushing, enzymolysis and vacuum drying on the grape seeds; and (2) firstly extracting the grape seed oil by using the supercritical carbon dioxide extraction technology, changing the extracting temperature and the extracting pressure after the grape seed oil is extracted, adding an entrainer, collecting a secondary extract, and obtaining a proanthocyanidin extract through separation and purification. By adopting the method, the total unsaturated fatty acid in the grape seed oil is 80-90 mass percent, and the proanthocyanidin in the proanthocyanidin extract is 90-98 mass percent. By adopting the method, the technologies of vacuum drying, cellulose enzymolysis and supercritical extraction are effectively integrated, so that low-temperature, low-energy, green, efficient and continuous extraction of the active ingredients in the grape seeds is realized. The grape seed oil and the proanthocyanidin extract are widely applied to the fields of medicine, food and health-care food, and have wide prospects.

The invention provides purple potato glutinous wheat noodles and a preparation method thereof. The purple potato glutinous wheat noodles are prepared from the following raw and auxiliary materials by weight: 10 to 40 grams of purple potato flour, 15 to 50 grams of glutinous wheat flour, 25 to 70 grams of ordinary flour, 1 to 2 grams of edible salt and the balance of water; and 100g of noodles comprise more than 15mg of cyanin, more than 0.0005mg of selenium, less than 20g of amylose, more than 70g of amylopectin and about 10g of coarse fiber. The purple potato glutinous wheat noodles prepared in the ratio have a lavender red color, noodles have a fresh aubergine color and smooth mouthfeel, and noodle soup has a purple color. The purple potato glutinous wheat noodles integrate health care and good mouthfeel, and are suitable to be consumed by various people.

The invention provides blueberry selenium-rich leaf fertilizer, a preparation method and an application method. The blueberry selenium-rich leaf fertilizer, 100% in total, is prepared from, by weight, 1.0-8.0% of one or combinations of urea and ammonium sulfate, 1.0-10.0% of at least one or combinations of ammonium polyphosphate, monoammonium phosphate and diammonium phosphate, 5.0-15.0% of one or combinations of potassium sulphate and monopotassium phosphate, 1.0-10.0% of trace elements, 1.0-1.5% of sodium selenite, 1.0-10.0% of fulvic acid, 0.5-5% of sugar alcohol substances, 0.5-1.0% of surface activated agents and the balance water. The application method includes the steps of spraying the fertilizer in the blueberry primary flowering period and spraying the fertilizer in the fruit expanding period before blueberry picking. The selenium-rich leaf fertilizer is developed for the characteristic that blueberry crops need the fertilizer; the selenium-rich leaf fertilizer is high in specificity and can promote blueberry growth and increase the selenium content, anthocyanin content and soluble protein content in blueberries.

The invention provides a preparation method for a grape seed proanthocyanidin extract. The preparation method comprises the following steps: firstly, preparing trichoderma koningii and aspergillus niger direct vat set; and secondly, adding water into grape seeds for grinding; adding glucose for mixing, sterilizing at high temperature, inoculating the bacteria and fermenting, centrifugally removing slag, carrying out ultrafiltration extraction, drying and other processes to obtain the grape seed proanthocyanidin extract.

The invention discloses a preparation method of a high-stability cellulose base natural edible anthocyan pigment compound. The compound consists of two parts of main materials and auxiliary materials, wherein the main materials comprise anthocyan as a functional component, and a nanometer/micron cellulose substance or a derivative colloidal solution thereof as a base material, wherein the nanometer/micron cellulose substance or the derivative colloidal solution thereof is used for loading the functional component; the auxiliary materials contain a stabilizing agent and a polysaccharide thickening agent. The preparation method comprises the following steps: at a lower rotating speed, dropwise adding an aqueous/organic solvent solution of anthocyan natural pigment into a pre-sheared base material mother solution using a cellulose base colloidal solution as a main component, and shearing the aqueous/organic solvent solution of the anthocyan natural pigment at a high speed so as to obtain the cellulose base pigment compound. The pigment compound is an aqueous-based anthocyan pigment compound emulsion which is post-processed and treated to be prepared into solid granules of the anthocyan pigment compound, the anthocyan pigment compounds of various forms can be well dispersed in water, the storage stability of the pigment can be improved, and the action period of the pigment can be prolonged.

The invention relates to a preparation method of Rhodiola rosea proanthocyanidin, comprising the following steps of: taking Rhodiola rosea rhizomes as a raw material, crushing the Rhodiola rosea rhizomes, sieving, carrying out a refluxing process for two hours by the use of ethanol at the concentration of 50% to obtain a proanthocyanidin crude extract; removing impurities from the crude extract, removing carbohydrates by high concentration ethanol, and removing lipid substances by petroleum ether; extracting by the use of ethyl acetate, separating high polymeric proanthocyanidin from oligomeric proanthocyanidin; finally purifying the high polymeric proanthocyanidin and the oligomeric proanthocyanidin by the use of a macroporous adsorption resin, followed by freeze-drying until the purity of the high polymeric proanthocyanidin and that of the oligomeric proanthocyanidin are respectively 95.0% and 88.3%. The method provided by the invention has advantages of simple technology and no pollution, and is easy to apply in industrial production; and the prepared proanthocyanidin has high purity and can be applied in natural pigment food additives as well as medical health-care raw materials which have an antioxidant function.

The invention provides an extraction method for high-purity high-oxidative-activity aronia melanocarpa procyanidine and belongs to the technical field of extraction and purification of procyanidine. The method comprises the following steps: as aronia melanocarpa is taken as a raw material, firstly, adopting a freeze drying technology to obtain aronia melanocarpa freeze-dried powder; then, extracting the aronia melanocarpa freeze-dried powder by adopting a supercritical CO2 extraction technology; finally, purifying the aronia melanocarpa procyanidine by adopting an AB-8 type macroporous adsorption resin purification technology. According to the extraction method, the freeze drying technology and the supercritical CO2 extraction technology are adopted so that the extraction efficiency is improved and the biological activity is kept; the macroporous resin purification technology is adopted so that the extraction purity of the aronia melanocarpa procyanidine is improved and the purity reaches to 90.1%-98.6%; the cleaning capability of the aronia melanocarpa procyanidine to DPPH (1,6-bis(diphenylphosphino)hexane) is within a range from 11.2 micrograms/milliliter to 15.6 micrograms/milliliter; the obtained procyanidine is high in purity and good in activity, can be directly applied to medicines and health-care foods.

The invention relates to a method for extracting golden buckwheat high polymeric proanthocyanidin and preparing oligomeric proanthocyanidin by carrying out catalytic degradation on the same. The method comprises the following steps: taking golden buckwheat root tuber as a raw material, taking an aqueous solution of ethanol as a solvent to leach the golden buckwheat root tuber, centrifugating the leaching liquor after volatilizing the ethanol, extracting a supernatant by an organic solvent, passing water phase raffinate through a macroporous resin, washing by an ethanol-water system, collecting an eluted solution, and carrying out decompression concentration and vacuum drying on the eluted solution to obtain a high polymer of the golden buckwheat proanthocyanidin; taking the aqueous solution of the ethanol as a solvent to prepare a solution of the high polymer of the golden buckwheat proanthocyanidin, carrying out an H2 degradation reaction under the high pressure in the presence of a palladium/carbon catalyst, filtering the reaction product, passing the filtrate through a macroporous resin column for adsorption and purification, eluting the aqueous solution of the ethanol, and carrying out decompression concentration and vacuum drying on the eluted solution to obtain the golden buckwheat oligomeric proanthocyanidin. The method can improve the yield of the high activity substance, namely the oligomeric proanthocyanidin, thereby expanding the application range of the golden buckwheat in the fields of medicaments, foods, cosmetics and health care products.

The invention relates to extraction of effective components of plants, and specifically provides a production method of extracting procyanidine from huckleberries. The method comprises the following steps: crushing a huckleberry raw material; extracting by adopting a negative pressure cavitation method; filtering by using an ultrafiltration organic membrane; purifying by using resin to obtain a procyanidine product. The method provided by the invention can be used for increasing the yield of the procyanidine and reducing the environmental pollution, and facilitating the factory production and the reduction of the production cost.

The invention discloses a method for extracting proanthocyanidins from Yunnan pine bark. The method comprises the following steps: (1) crushing Yunnan pine bark, and then adding cellulase and acetic acid-sodium acetate buffer solution to carry out enzymolysis on the crushed bark; (2) carrying out ultrasonic treatment in an enzymolysis process; (3) adding an ethanol solution into the enzymatic hydrolysis suspension, uniformly mixing, and carrying out ultrasonic treatment again; (4) separating and purifying the crude extract to obtain the proanthocyanidins extract. The extraction method is simple in process and convenient to operate, the yield of proanthocyanidins is increased by 15%-25% higher than that of a common solvent extraction method, and the method is suitable for industrial production.

The invention aims at providing a method for extracting oligomeric procyanidine from grape seeds. The method comprises the following steps: pulverizing defatted grape seeds, adding ethyl acetate and carrying out ultrasonic extraction to obtain a crude extract (shown in the specification), adding cellulose for enzymolysis, extracting twice with ethyl acetate to respectively obtain a crude extract (shown in the specification) and another crude extract (shown in the specification), mixing the crude extracts and purifying to obtain oligomeric procyanidine of which the purity is above 95%. According to the method, by many rounds of extraction and low-temperature preservation of extracts in a dark place, the long-term exposure of oligomeric procyanidine is avoided and thus the oxidation of oligomeric procyanidine and the degradation of excessive procyanidine into inactive monomers are decreased; the subsequent purification process steps of the method disclosed by the invention are simpler, the conventional steps of purifying with macroporous adsorption resin, HPLC refining and the like in the field are omitted, a large amount of solvents are saved and environment friendliness is achieved.

The invention discloses a method for efficiently and quickly extracting grape seed oil and proanthocyanidin, which comprises the following steps: 1) pulverizing grape seed, adding the pulverized grape seed into a subcritical extraction kettle, introducing a nonpolar organic reagent, dynamically extracting at 20-80 DEG C under the pressure of 0.3-5 MPa for 20-80 minutes, recycling the reagent from the extract, and adding atlapulgite and activated carbon for decolorization and deodorization, thereby obtaining the grape seed oil; and 2) introducing a polar organic reagent or water into the extraction residue, dynamically extracting at 20-150 DEG C under the pressure of 0.3-8 MPa for 30-90 minutes, recycling the reagent from the extract, adding into a macroporous resin column for adsorption, carrying out gradient elution with an ethanol solution, concentrating the eluate, and carrying out spray drying to obtain the proanthocyanidin. The method disclosed by the invention has the advantages of low energy consumption, low solvent volume and high product yield, and is suitable for industrialized popularization.

The invention discloses low-AGEs inulin cookies. The cookies are prepared from, by weight, 40-60 parts of low-gluten flour, 10-20 parts of corn flour, 5-15 parts of egg yolk, 10-20 parts of butter, 5-10 parts of white sugar, 10-20 parts of nature inulin, and 0.1-0.5 part of litchi procyanidine extractive. The low-AGEs inulin cookies belong to the technical field of food processing. The low-AGEs inulin cookies have the advantages of reducing production of AGEs in cookies and reaching the effects of maintaining health and delaying senescence. The low-AGEs inulin cookies are more suitable for the middle aged and elderly people and diabetics.

The invention relates to a method for preparing radish red pigment homopolymer and radish proanthocyanidin from red-core radishes, belonging to the technical field of preparation of natural red pigments and proanthocyanidin. The method comprises the following steps: by using red-core radishes as the raw material, extracting, removing proteins, removing glucoraphanin, purifying by ultrafiltration,concentrating, and carrying out spray drying to obtain the radish red pigment homopolymer and radish proanthocyanidin products. The invention has the advantages of great convenience, simple technique, wide raw material sources, and cheap and accessible raw materials; all the materials in the production process can be recycled, so that the material resources are fully utilized and no waste is discharged, and thus, the invention also has the advantages of energy saving and low production cost; and the products have the advantages of high purity, no peculiar smell of radishes, favorable solubility and the like. The invention can be widely used for preparing radish red pigment homopolymer and radish proanthocyanidin from red-core radishes. The products prepared by the method provided by the invention can be widely used in the industries of food, medicines, health products, cosmetics, dyes and the like.

The invention specifically discloses a tea tree MYB transcription factor CsAN1 and an application thereof in regulation of anthocyanin metabolism. A nucleotide sequence of the tea tree MYB transcription factor CsAN1 is represented as SEQ ID NO:1. CsAN1 is connected with a 35S promoter and then is connected to a pBI121 carrier, meditated transformation of tobaccos is realized by agrobacteria GV3101, meanwhile, the gene as well as genes CsGL3 and CsTTG1 capable of forming compounds with the gene is used for transforming the tobaccos respectively, leaves of overexpressed tobacco plants turn red, anthocyanin content detected by a spectrophotometer is remarkably increased, and two main pigments including cyanidine and delphinidin are detected through HPLC (high performance liquid chromatography).

The invention discloses a mulberry, blueberry and plant beverage rich in anthocyanidins, and a preparation method thereof. Every 1000ml of the beverage comprises 6-35g of a mulberry juice concentrate, 4-10g of a blueberry juice concentrate, 1g of Sedum aizoon L. extract, 1g of a lime juice concentrate, 0.01-0.25g of stevioside, 20-30g of white granulated sugar, 1g of citric acid, 0.4-0.5g of carboxymethyl cellulose, and the balance of water. The beverage has the advantages of reasonable formula, no essences, pigments or antiseptics, keeping of the sweetness, and effective reduction of heat energy, and is a healthy drink; mulberry and blueberry are respectively rich in anthocyanidins, Sedum aizoon L. contains various amino acids and vitamins, so mulberry, blueberry and Sedum aizoon L. can be compounded to clear heat, detoxify, nourish heart, relieve asthma, improve eyesight, remove free radicals, prevent ageing and improve immunity; and the beverage has natural color, is clear and transparent, has refreshing mouthfeel, and is suitable for people of all ages.

The present invention relates to a composition with function of beautifying face and its application. The composition contains no any chemical synthetic components and additive, and is composed of 15-500 weight portions of isoflavone which is soybean isoflavone and 20-500 weight portions of protocyanidine which is grape-seed extract protocyanidine. The composition has the good synergistic action for beautifying and nourishing face, and said composition also relates to the application for preparing medicines for curing menopausal syndrome, osteoporosis, cancers, cardiovascular disease and for raising and regulating immunity.

The invention discloses a method for quickly extracting vitis amurensis rupr seed procyanidin.The method mainly includes the steps that vitis amurensis rupr seeds are separated from pomace after wine making, vitis amurensis rupr seed powder is obtained through freezing drying, smashing, degreasing and screening, a 50-70% ethanol aqueous solution is added as an extraction solvent according to the material-liquid ratio (g/mL) of 1:(15-25), 0.3-0.5% (w/v) sodium hexametaphosphate is added at the same time as an assistant agent, the mixture is evenly stirred, passes a high-voltage pulse electric field and is centrifuged under the conditions that the number of pulses is 8-12 and the intensity of the electric field is 20-30kV/cm, supernatant is subjected to vacuum concentration and freezing drying, and a target product is obtained.Compared with a conventional extraction method, the technology can obviously increase the procyanidin field, shorten extraction time and reduce energy consumption, and the high-voltage pulse electric field serves as a non-heat-processing technology so that the activity of procyanidin can be well kept.