7 results about "Betacyanins" patented technology

The application discloses a Cymbidium cultivation method capable of controlling gradual color change and a novel color Cymbidium, and relates to the technical field of plant genetic engineering. The method comprises the following steps: constructing a fusion expression vector containing a P2A self-cleavage peptide, introducing the vector into Agrobacterium to obtain an engineered Agrobacterium, introducing the engineered Agrobacterium into a Cymbidium receptor material by using an Agrobacterium-mediated transformation method, and culturing, screening positive transformants by observing red pigment accumulation under environmental light, and adjusting transformation conditions to realize the synergistic accumulation or different expression levels of anthocyanins and betalains. The method realizes independent expression of double genes from a single mRNA, realizes device-free screening by combining RUBY visual marking, realizes color gradient control by transformation condition regulation, can realize stable coexistence of anthocyanins and betalains in the same tissue of Cymbidium, creates novel colors such as a purplish red color system or a gradual color, and shortens the transformation period to 5 days.

The present application provides a betalain-cyanidin composite pigment composition, a dyeing agent and application, the composition is combined by cyanidin freeze-dried powder and betalain freeze-dried powder according to the mass ratio of 5:1-5. The betalain-cyanidin composite dyeing agent formed by the composition has good temperature stability and pH stability, and strong coloring power, and can be widely applied to product materials containing kapok, silk, cotton, hemp and the like.

The invention discloses a transcriptional activation type RUBY visual virus infection system as well as a method and application thereof. The system is based on a betacyanin biosynthesis pathway, a transcriptional activation system is utilized, and a yeast GAL4 DNA binding domain (BD) and a herpes virus VP16 transcriptional activation domain (AD) are fused and constructed in a virus vector; when the virus infects expressed transgenic tobacco, the expression of the RUBY whole gene can be activated, so that a red signal is generated. The construction method of the system is clear, complex instruments are not needed, an efficient and convenient tool platform is provided for protein function analysis, host factor screening and infection mechanism exploration in virology research, and good expandability is achieved.

This invention relates to the field of plant genetic engineering, specifically providing a method for regulating the synthesis of betaine in bougainvillea bracts. BbMYB69 Genes and their encoded proteins and their applications. This invention constructs a gene based on VIGS technology. BbMYB69 Gene-specific silencing vector pTRV2- BbMYB69 After Agrobacterium-mediated infection of bougainvillea bracts, the incidence of disease can be significantly reduced. BbMYB69 The expression level of the gene caused a significant fading of the red bracts of bougainvillea, and the betalain content in the bracts was significantly lower than that in the control group. This invention clarifies for the first time that... BbMYB69 BbMYB69 The gene's core regulatory function in betalain synthesis in bougainvillea bracts provides a novel gene target and technical means for molecular breeding of bougainvillea flower color. At the same time, it provides important evidence for the study of transcriptional regulation mechanism of betalain synthesis in caryophyllales plants, and has significant scientific research value and industrial application prospects.

This invention belongs to the field of biopolymer material preparation technology, and discloses a betaine-loaded composite hydrogel, its preparation method and application, including the following steps: (1) heat-treating an ovalbumin solution under acidic conditions to induce its self-assembly into an ovalbumin amyloid fiber dispersion; (2) mixing the ovalbumin amyloid fiber dispersion with a gelatin solution, stirring and dissolving to obtain a matrix mixture; (3) adding betaine and transglutaminase to the obtained matrix mixture, reacting at 37~55℃ for 0.5~3h, and then cooling and fixing to obtain the betaine-loaded composite hydrogel. This invention prepares a composite hydrogel with a dense three-dimensional network structure and high mechanical strength, while improving the photothermal stability of betaine. The obtained composite hydrogel achieves efficient encapsulation and sustained-release control of betaine at a low addition amount, and can be widely used in functional foods and biopharmaceutical industries.

The invention discloses a separated RUBY visual virus infection system as well as a construction method and application thereof. According to the system, a key gene of a betacyanin biosynthetic pathway is split into two parts: CYP76AD1 and a GT gene are connected in series through a 2A peptide sequence and are constructed in a plant stable expression vector, and a stably inherited transgenic plant is obtained in nicotiana benthamiana; and cloning another key gene DODA into a plant virus vector to construct a recombinant virus expression vector. According to the method, real-time, lossless and in-situ visual observation of the virus infection and movement process is achieved, the system construction method is clear, complex instruments are not needed, an efficient and convenient tool platform is provided for protein function analysis, host factor screening and infection mechanism exploration in virology research, and good expandability is achieved.

The invention relates to the technical field of plant genetic engineering, and particularly provides molecular cloning of a rice endosperm specific promoter pROLM24 and application of the rice endosperm specific promoter pROLM24. Three tissue-specific promoters with nucleotide sequences as shown in SEQ ID NO.1-3 are separated from a rice alcohol-soluble protein gene, the three tissue-specific promoters are fused with an RUBY visual marker, and a result shows that the content of betacyanin in an expression product is measured to be up to 2.9% of the dry weight of rice seeds. The method has very high application value in the field of plant synthetic biology. The invention further discloses application of the promoter in the aspects of improving crop seed quality, improving crop traits, cultivating new varieties of transgenic plants by using key elements of the promoter or expressing high value-added proteins by using a seed bioreactor and the like.