35 results about "Betacyanins" patented technology

The invention relates to the field of betacyanin content detection, and discloses a method for rapidly detecting betacyanin content based on spectrophotometry, which comprises the following steps: experimental operation: preparing samples into standard solutions with different concentrations, carrying out full-spectrum scanning on the standard solutions by using a spectrophotometer, and determining the content of betacyanin by using a spectrophotometer; recording absorbance values under different wavelengths to form a spectral data matrix; preprocessing: preprocessing the spectral data matrix to obtain basic data; first analysis: analyzing the basic data by adopting a multivariate correction technology so as to obtain first data; and second analysis: analyzing the basic data by adopting a spectral feature extraction and mode recognition technology. A complete detection process is formed from data acquisition, preprocessing and analysis of different technologies to final fusion and integration, each step provides better data or more accurate analysis results for subsequent steps, and rapid and accurate detection of the betacyanin content is jointly realized.

The invention relates to the technical field of preparation of food additives, in particular to a betacyanin microcapsule as well as a preparation method and application thereof. The preparation method comprises the following steps: jointly dissolving sodium alginate and soybean protein isolate in water to obtain an SA-SPI compound; the SA-SPI compound, CaCO3 and betacyanin are mixed, and a water phase is formed; mixing liquid paraffin, glacial acetic acid and Tween 80 to obtain an acetic acid-containing oil phase; mixing the water phase with the acetic acid-containing oil phase to form a water-in-oil emulsion, and standing to form calcium alginate gel beads; the calcium alginate gel beads are subjected to pre-freezing-freeze-drying treatment, and the betacyanin microcapsules are obtained. The prepared betacyanin microcapsule has the characteristics of controllable particle size, high stability and easiness in industrialization, and the application range of betacyanin is widened. In addition, the preparation method disclosed by the invention is safe and simple to operate, has the advantages of low cost and high benefit, and can be directly applied to large-scale production.

The invention provides a pitaya wine processing technology. The pitaya wine processing technology is optimized aiming at the problems that pigments are easy to degrade, the fermentation efficiency is low, and the flavor is single in a traditional technology. According to the raw material pretreatment, ultrasonic cleaning (0.05% sodium hexametaphosphate solution) is combined with metal ion control (Fe2 / Cu2 is less than or equal to 0.1 mg / L), and the juice yield is increased through composite enzymolysis (pectinase and cellulase). In the low-temperature fermentation stage, the period is shortened to 10 hours by utilizing fed-batch strain activation, and the retention rate of characteristic aroma is remarkably improved by combining pulse ultrasound and nitrogen protection (the oxygen content is less than or equal to 0.5 ppm); and through infrared monitoring and gradient cooling, accurate control of the alcoholic strength being 12% vol and the residual sugar being less than or equal to 4g / L is realized. In the aging stage, nitrogen is adopted to isolate oxygen (high-purity nitrogen is greater than or equal to 99.99%) and a KPA stabilizer, so that the degradation rate of betacyanin is less than or equal to 10%. Finally, the stability is improved through ceramic membrane filtration, cold stabilization and pasteurization, and the product is purplish red in color and luster and complex in flavor and has industrial application potential.

The invention discloses a chicken freshness detection method and system combining a colorimetric gel label and a convolutional neural network, and belongs to the technical field of food safety detection. An indicating gel dot matrix label is prepared from curcumin, anthocyanin and betacyanin, the optimal load concentration of curcumin, anthocyanin and betacyanin is optimized and determined, and sensitive color response is generated to volatile biogenic amine gas such as dimethylamine and trimethylamine; gel label images are collected, the images are divided into fresh samples, secondary fresh samples and decayed samples in combination with the TVB-N content, the samples are subjected to data enhancement and then input into an OfficientNet-B0 model for training, and efficient classification is achieved. According to the method, a detection system combining the natural pigment-based multi-channel colorimetric gel and the lightweight convolutional neural network is constructed, lossless, rapid and intelligent detection of the freshness of the fresh chicken is realized, and a green and efficient solution is provided for real-time monitoring of the freshness of food and application of a mobile terminal.

Pigment compositions comprising betalain pigment molecules are provided. Compositions comprising betanin and betanidin are also provided as are compositions comprising less than 5% sugars by weight. Cells comprising at least one copy of an exogenous tyrosine hydroxylase gene, at least one copy of an exogenous L-DOPA oxidase gene, at least one copy of an exogenous DOPA 4,5-dioxygenase (DOD) gene, at least one copy of an exogenous NADPH cytochrome P450 reductase gene, and at least one copy of a cyclo- DOPA-5-O-glucosyltransferase gene integrated into the genome of the cell are also provided.

Aspects of the disclosure relate to tyrosine hydroxylases, including engineered enzymes, and their use in catalyzing chemical reactions to produce betalain. Additional aspects of the disclosure relate to genetic modifications of host cells to improve betalain production.

The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 plasmid taking a Leu2 gene as a selection marker, and belongs to the technical field of gene engineering. According to the CRISPR / Cas9 plasmid developed by the invention, Cas9 protein and components required for transcription of sgRNA are constructed on one plasmid, and the complete CRISPR / Cas9 system assembly can be realized only by one-step construction. And the plasmid is used for knocking out an endogenous gene Yl4HPPD (YALI1B28454g) of the yarrowia lipolytica, and meanwhile, an exogenous gene EcaroGS180F from escherichia coli is introduced, so that the betacyanin production capacity of the strain BE12 is further improved. According to the invention, exogenous gene knock-in is successfully realized, the high-yield betacyanin strain BE26 is obtained, and the total yield of betacyanin is 66.1 mg / L after the strain is cultured in an SC liquid culture medium for 48 hours.

The invention relates to a quantitative detection method of betacyanin. The invention provides a betacyanin quantitative detection method which comprises the following steps: (1) establishing a standard curve: preparing a gradient standard solution from a betacyanin standard sample, and adding the gradient standard solution on an elisa plate to measure a light absorption value; obtaining a standard curve according to the absorbance, and obtaining a linear regression equation according to the standard curve and the Lambert-Beer law; (2) quantitative detection: pre-washing a to-be-detected sample to obtain an extracting solution, removing impurities of the extracting solution, diluting to obtain a loading solution, adding the loading solution to the ELISA plate, detecting to obtain a light absorption value of the to-be-detected sample, and substituting the light absorption value of the to-be-detected sample into the linear regression equation for calculation to obtain the concentration of the betacyanin in the to-be-detected sample. Finally, the content of betacyanin in the sample is obtained through calculation according to the formula # imgabs0 #, the quantitative detection method is faster, simpler, more convenient and more accurate, the method can be applied to the samples containing grease, and batch determination of the samples is achieved.

The application discloses a Cymbidium cultivation method capable of controlling gradual color change and a novel color Cymbidium, and relates to the technical field of plant genetic engineering. The method comprises the following steps: constructing a fusion expression vector containing a P2A self-cleavage peptide, introducing the vector into Agrobacterium to obtain an engineered Agrobacterium, introducing the engineered Agrobacterium into a Cymbidium receptor material by using an Agrobacterium-mediated transformation method, and culturing, screening positive transformants by observing red pigment accumulation under environmental light, and adjusting transformation conditions to realize the synergistic accumulation or different expression levels of anthocyanins and betalains. The method realizes independent expression of double genes from a single mRNA, realizes device-free screening by combining RUBY visual marking, realizes color gradient control by transformation condition regulation, can realize stable coexistence of anthocyanins and betalains in the same tissue of Cymbidium, creates novel colors such as a purplish red color system or a gradual color, and shortens the transformation period to 5 days.

The invention discloses a method for extracting betacyanin with high antioxidant activity from pitaya peel. Fresh pitaya peel is used as a raw material, the betacyanin is extracted by adopting an ethanol extraction combined ultrasonic method, powder is obtained through dilution, suction filtration, centrifugation, rotary evaporation and vacuum freeze drying, and the betacyanin with the content of about 133mg / 100g is extracted; the obtained betacyanin has high oxidation resistance and can effectively replace artificially synthesized pigments. The method provides theoretical basis and technical support for efficient extraction of natural pigments, resource utilization of pitaya byproducts and functional development of betacyanin.

The present application provides a betalain-cyanidin composite pigment composition, a dyeing agent and application, the composition is combined by cyanidin freeze-dried powder and betalain freeze-dried powder according to the mass ratio of 5:1-5. The betalain-cyanidin composite dyeing agent formed by the composition has good temperature stability and pH stability, and strong coloring power, and can be widely applied to product materials containing kapok, silk, cotton, hemp and the like.

The invention provides a promoter pchsA derived from petunia, a gene expression cassette of betacyanin and application of the gene expression cassette, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the promoter pchsA is shown as SEQ ID No.1; according to the invention, a petunia organ specific promoter pchsA is utilized to construct a gene expression cassette of the betacyanin, so that heterologous synthesis of the betacyanin is successfully driven in tobacco, and expression and accumulation of the betacyanin in tissues of roots, stems, leaves and flowers of the tobacco are realized. According to the invention, a new transgenic tobacco germplasm capable of efficiently accumulating the betacyanin is created, a new material is provided for plant bioreactor production of the high-value pigment (betacyanin), and meanwhile, a method for rapidly, intuitively and visually detecting the activity of the promoter is established.

The invention provides a method for extracting selenium-containing betacyanin from selenium-rich pitaya, and relates to the technical field of natural product extraction, the method comprises the following steps: (1) peel pretreatment, (2) pulp pretreatment, (3) mixing, (4) extraction, (5) elution, and (6) concentration and drying. The selenium-rich betacyanin is extracted from the selenium-rich pitaya, the method has the advantages of being simple in process, easy to operate, high in extraction yield and the like, and the betacyanin obtained through the method is rich in selenium and high in oxidation resistance.

This invention relates to the field of medical materials, and discloses a hydrogel dressing with dual-response properties, its preparation method, and its applications. The dressing is composed of a visualization indicator layer and a pH / temperature-responsive treatment layer, composited through a gradient cross-linking interface: the indicator layer is composed of sodium alginate, PVP, and betalain, enabling visual monitoring of wound pH through pigment color development; the treatment layer uses gelatin / carboxymethyl cellulose as a matrix, loaded with chlorogenic acid and notoginseng saponins, forming a porous scaffold with a pore size of 50-200 μm, possessing dual pH / temperature-responsive drug release capabilities. Ca²⁺ is used in the preparation process. + The alternating spraying strategy of genipin constructs a gradient interface, which, combined with low-temperature plasma treatment, forms an interpenetrating network structure. The interface bonding strength is enhanced through secondary wet curing. This dressing achieves a synergistic effect of precise drug release and infection early warning through microenvironment response. It is especially suitable for the treatment of diabetic wound infections and has multiple functions such as real-time monitoring, intelligent drug release, and promotion of tissue repair.

Contemplated compositions and methods employ betalains for treatment of various conditions, and especially osteoarthritis, sinusitis, contact dermatitis, acne, an allergic condition, reduced mental alertness, reduced physical strength, reduced physical endurance, and / or impaired mood.

The invention discloses a transcriptional activation type RUBY visual virus infection system as well as a method and application thereof. The system is based on a betacyanin biosynthesis pathway, a transcriptional activation system is utilized, and a yeast GAL4 DNA binding domain (BD) and a herpes virus VP16 transcriptional activation domain (AD) are fused and constructed in a virus vector; when the virus infects expressed transgenic tobacco, the expression of the RUBY whole gene can be activated, so that a red signal is generated. The construction method of the system is clear, complex instruments are not needed, an efficient and convenient tool platform is provided for protein function analysis, host factor screening and infection mechanism exploration in virology research, and good expandability is achieved.

Based on three key enzymes of a betacyanin synthetic route, a plant expression vector tool capable of simply, conveniently and quickly detecting the transcriptional activity of the bidirectional promoter at high throughput is developed. By utilizing the tool provided by the invention, the bidirectional transcriptional activity can be judged by only constructing the candidate DNA sequence to the specified position of the vector and observing whether red color appears in the tissue through transient expression or stable genetic transformation, so that the method is simple, convenient and efficient.

The invention relates to a betacyanin extraction method, in particular to a betacyanin ultrasonic extraction method based on a red beet nutritional ingredient accumulation rule. The problems that impurities are too high and the extraction amount is small in a betacyanin extraction project formed by a common technology are solved. Comprising the following steps: adding an extracting agent into freeze-dried powder, carrying out ultrasonic extraction, filtering with gauze after extraction, carrying out suction filtration with filter paper, removing residual dietary fibers in beet juice, centrifuging the beet juice in a centrifugal machine for 10 minutes at the speed of 12,000 r / min after removal, taking 665 mu L of supernate, and fixing the volume to 10 mL. According to the method disclosed by the invention, relatively high extraction efficiency of the betacyanin is ensured, and certain data support is provided for stabilization and industrial research of the betacyanin.

The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and / or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as Mirabilis jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.

This invention relates to the field of plant genetic engineering, specifically providing a method for regulating the synthesis of betaine in bougainvillea bracts. BbMYB69 Genes and their encoded proteins and their applications. This invention constructs a gene based on VIGS technology. BbMYB69 Gene-specific silencing vector pTRV2- BbMYB69 After Agrobacterium-mediated infection of bougainvillea bracts, the incidence of disease can be significantly reduced. BbMYB69 The expression level of the gene caused a significant fading of the red bracts of bougainvillea, and the betalain content in the bracts was significantly lower than that in the control group. This invention clarifies for the first time that... BbMYB69 BbMYB69 The gene's core regulatory function in betalain synthesis in bougainvillea bracts provides a novel gene target and technical means for molecular breeding of bougainvillea flower color. At the same time, it provides important evidence for the study of transcriptional regulation mechanism of betalain synthesis in caryophyllales plants, and has significant scientific research value and industrial application prospects.

This invention belongs to the field of biopolymer material preparation technology, and discloses a betaine-loaded composite hydrogel, its preparation method and application, including the following steps: (1) heat-treating an ovalbumin solution under acidic conditions to induce its self-assembly into an ovalbumin amyloid fiber dispersion; (2) mixing the ovalbumin amyloid fiber dispersion with a gelatin solution, stirring and dissolving to obtain a matrix mixture; (3) adding betaine and transglutaminase to the obtained matrix mixture, reacting at 37~55℃ for 0.5~3h, and then cooling and fixing to obtain the betaine-loaded composite hydrogel. This invention prepares a composite hydrogel with a dense three-dimensional network structure and high mechanical strength, while improving the photothermal stability of betaine. The obtained composite hydrogel achieves efficient encapsulation and sustained-release control of betaine at a low addition amount, and can be widely used in functional foods and biopharmaceutical industries.

The invention provides a combined gene RUBY capable of generating betacyanin through expression in sweet potatoes and application thereof, the combined gene RUBY is formed by sequentially connecting a CYP76AD1 gene, a DODA gene and a GT gene in series through a 2A peptide sequence capable of being automatically cut through coding, the nucleotide sequence of the CYP76AD1 gene is shown as SEQ ID NO.1, the nucleotide sequence of the DODA gene is shown as SEQ ID NO.3, and the nucleotide sequence of the GT gene is shown as SEQ ID NO.5. The invention further provides a preparation method of the combined gene RUBY capable of generating betacyanin through expression in sweet potatoes and application of the combined gene RUBY capable of generating betacyanin through expression in sweet potatoes. According to the invention, a combined gene participating in betacyanin synthesis is obtained, a combined coding region of the combined gene is constructed into an enhanced over-expression vector, and after the vector is transferred into sweet potatoes, three key genes participating in betacyanin biosynthesis in obtained transgenic sweet potatoes have obvious expression compared with non-transgenic sweet potatoes so as to form betacyanin; and different tissues of the transgenic plant are pink. The method disclosed by the invention has important guiding significance and good production and application values for directionally breeding novel functional sweet potato varieties through a genetic engineering technology.

Aspects of the disclosure relate to tyrosine hydroxylases, including engineered enzymes, and their use in catalyzing chemical reactions to produce betalain. Additional aspects of the disclosure relate to genetic modifications of host cells to improve betalain production.

The invention discloses a separated RUBY visual virus infection system as well as a construction method and application thereof. According to the system, a key gene of a betacyanin biosynthetic pathway is split into two parts: CYP76AD1 and a GT gene are connected in series through a 2A peptide sequence and are constructed in a plant stable expression vector, and a stably inherited transgenic plant is obtained in nicotiana benthamiana; and cloning another key gene DODA into a plant virus vector to construct a recombinant virus expression vector. According to the method, real-time, lossless and in-situ visual observation of the virus infection and movement process is achieved, the system construction method is clear, complex instruments are not needed, an efficient and convenient tool platform is provided for protein function analysis, host factor screening and infection mechanism exploration in virology research, and good expandability is achieved.

The invention discloses a gene combination for enhancing synthesis of betacyanin in crop seed endosperm and application of the gene combination. The gene combination comprises a CYP76AD1 gene, a DODA1 gene, a DOPA5GT gene and an ADH [alpha] gene. According to the gene combination, through co-expression of a tyrosine synthetase gene and a betacyanin synthesis gene, the content of betacyanin in endosperm is remarkably increased. CYP76AD1, DODA1 and DOPA5GT genes are used for constructing a double expression cassette of endosperm specific expression betacyanin synthesis expression cassette RUBY and endosperm specific expression ADH alpha or a single expression cassette of endosperm specific expression CYP76AD1, DODA1, DOPA5GT and ADH alpha, the expression cassette is transferred into a plant transformation vector, the plant transformation vector is introduced into a breeding crop, and the breeding crop is obtained. The transgenic crop with endosperm specific efficient accumulation of betacyanin can be directly used as a functional food or a betacyanin biological manufacturing raw material.

The invention relates to the technical field of food processing, in particular to a fermentation type beet root pigment and a preparation method and application thereof. The method comprises the following steps: slicing and crushing beet roots, inoculating a proper amount of specific strains, fermenting for a certain time in an intermittent multi-frequency ultrasonic mode, collecting fermentation liquor, adding a proper amount of maltodextrin and acid substances, and obtaining the fermented beet root pigment powder with dual functions of reducing blood sugar and uric acid by adopting a spray drying mode. Through the synergistic effect of a specific strain combination and an ultrasonic fermentation process, the content of betacyanin in a fermentation product is remarkably increased, and the beet root pigment powder has good inhibitory activity of alpha-amylase, alpha-glucosidase and xanthine oxidase. The prepared pigment powder not only has good stability, can be used as a natural pigment for baked foods such as bread, but also can effectively reduce the starch digestibility of the bread, and has a wide application prospect in preparation of functional products for reducing blood sugar and uric acid.

The invention discloses a method for creating a new germplasm of sweet corn with high betacyanin content. According to the method, a carrier containing a single expression cassette (CDGA) of CYP76AD1, DODA1, DOPA5GT and ADHalpha driven by a corn endosperm specific promoter PGt1 is converted into corn, the converted corn of endosperm specific accumulation betacyanin is obtained through screening, and the result shows that the efficiency of expressing betacyanin by the CDGA in the corn is obviously superior to that of a double expression cassette (CDG + A). Furthermore, a new transgenic sweet corn germplasm (ZCDGA) with endosperm specific efficient accumulation of betacyanin is obtained through a cross breeding technology, and the betacyanin content, the sugar content and the yield of the new transgenic sweet corn germplasm are remarkably improved compared with those of parents. According to the method, betacyanin is successfully and efficiently synthesized in the corn endosperm, a new sweet corn germplasm with high nutritional value and excellent eating quality is created, and a new thought and method are provided for biological enhancement of nutritional functional crops.

The invention relates to the technical field of plant genetic engineering, and particularly provides molecular cloning of a rice endosperm specific promoter pROLM24 and application of the rice endosperm specific promoter pROLM24. Three tissue-specific promoters with nucleotide sequences as shown in SEQ ID NO.1-3 are separated from a rice alcohol-soluble protein gene, the three tissue-specific promoters are fused with an RUBY visual marker, and a result shows that the content of betacyanin in an expression product is measured to be up to 2.9% of the dry weight of rice seeds. The method has very high application value in the field of plant synthetic biology. The invention further discloses application of the promoter in the aspects of improving crop seed quality, improving crop traits, cultivating new varieties of transgenic plants by using key elements of the promoter or expressing high value-added proteins by using a seed bioreactor and the like.

The invention belongs to the technical field of plant treatment, and particularly relates to a method for increasing the betacyanin content of suaeda heteroptera cluster buds and application thereof.In the process of replacing a culture medium after primary culture of the suaeda heteroptera cluster buds, NaCl is added into the culture medium or the pH of the culture medium is adjusted to 6.5-7.0, and the plant hormone concentration of a cluster bud induced proliferation culture medium is obtained; the influence of environmental conditions on betacyanin biosynthesis is determined.

The invention provides a method for rapidly obtaining a high-yield betacyanin yarrowia lipolytica strain, and belongs to the technical field of genetic engineering. According to the invention, the chassis BE00 suitable for producing tyrosine derivatives is constructed, the construction process of the yarrowia lipolytica chassis in which a high-copy betacyanin pathway gene is externally introduced is accelerated, and a stable and high-yield betacyanin production chassis BE12 is obtained.