Extraction method for high-purity high-oxidative-activity aronia melanocarpa procyanidine

A technology for proanthocyanidins of aronia and aronia nigra, applied in the field of extraction and purification of proanthocyanidins, can solve the problems of inactivation, low purity, and low extraction rate of proanthocyanidins, and achieves maintaining biological activity, high purity, and avoiding the environment. Effects of problems and security concerns

Active Publication Date: 2013-12-18
吉林省轻工业设计研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a process capable of industrially extracting high-purity, high-antioxidative activity Aronia proanthocyanidins, and at the same time provide the best freeze-dry...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Select mature and pest-free raw materials of Sorbus adenocarpus, use a tissue grinder to beat the pulp, put the pulp in -40°C for 4 hours, put it on a freeze-drying tray, the material thickness is 3-4mm, and the temperature of the cold trap is -50°C , and then freeze-dried on a freeze dryer for 18 hours. After drying, the freeze-dried powder is pulverized with a pulverizer, and then passed through a 100-mesh sieve to obtain the freeze-dried powder of Aronia nigra.

[0020] Take 500g of freeze-dried powder and carry out supercritical CO 2 Extraction, extraction pressure 35MPa, extraction time 90min, extraction temperature 45°C, solid-liquid ratio 1:1.08, 60% ethanol as entrainer, extract proanthocyanidins under these conditions.

[0021] Supercritical CO 2 The extract was purified with AB-8 macroporous adsorption resin, the diameter of the adsorption column was 24mm, the ratio of height to diameter of the adsorption column was 10, the concentration of the stock solutio...

Embodiment 2

[0023] Select mature and pest-free raw materials of Sorbus adenocarpus, use a tissue grinder to beat the pulp, put the pulp in -40°C for 4 hours, put it on a freeze-drying tray, the material thickness is 3-4mm, and the temperature of the cold trap is -50°C , and then freeze-dried on a freeze dryer for 18 hours. After drying, the freeze-dried powder is pulverized with a pulverizer, and then passed through a 100-mesh sieve to obtain the freeze-dried powder of Aronia nigra.

[0024] Take 500g of freeze-dried powder and carry out supercritical CO 2Extraction, extraction pressure 30MPa, extraction temperature 45°C, extraction time 90min, solid-liquid ratio 1:1.08, 60% ethanol as entrainer, extract proanthocyanidins under these conditions.

[0025] Supercritical CO 2 The extract was purified with AB-8 macroporous adsorption resin, the diameter of the adsorption column was 24mm, the ratio of height to diameter of the adsorption column was 10, the concentration of the stock solution...

Embodiment 3

[0027] Select mature and pest-free raw materials of Sorbus adenocarpus, use a tissue grinder to beat the pulp, put the pulp in -40°C for 4 hours, put it on a freeze-drying tray, the material thickness is 3-4mm, and the temperature of the cold trap is -50°C , and then freeze-dried on a freeze dryer for 18 hours. After drying, the freeze-dried powder is pulverized with a pulverizer, and then passed through a 100-mesh sieve to obtain the freeze-dried powder of Aronia nigra.

[0028] Take 500g of freeze-dried powder and carry out supercritical CO 2 Extraction, extraction pressure 40MPa, extraction time 90min, extraction temperature 45°C, solid-liquid ratio 1:1.08, 60% ethanol as entrainer, extract proanthocyanidins under these conditions.

[0029] Supercritical CO 2 The extract was purified with AB-8 macroporous adsorption resin, the diameter of the adsorption column was 24mm, the ratio of height to diameter of the adsorption column was 10, the concentration of the stock solutio...

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PUM

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Abstract

The invention provides an extraction method for high-purity high-oxidative-activity aronia melanocarpa procyanidine and belongs to the technical field of extraction and purification of procyanidine. The method comprises the following steps: as aronia melanocarpa is taken as a raw material, firstly, adopting a freeze drying technology to obtain aronia melanocarpa freeze-dried powder; then, extracting the aronia melanocarpa freeze-dried powder by adopting a supercritical CO2 extraction technology; finally, purifying the aronia melanocarpa procyanidine by adopting an AB-8 type macroporous adsorption resin purification technology. According to the extraction method, the freeze drying technology and the supercritical CO2 extraction technology are adopted so that the extraction efficiency is improved and the biological activity is kept; the macroporous resin purification technology is adopted so that the extraction purity of the aronia melanocarpa procyanidine is improved and the purity reaches to 90.1%-98.6%; the cleaning capability of the aronia melanocarpa procyanidine to DPPH (1,6-bis(diphenylphosphino)hexane) is within a range from 11.2 micrograms/milliliter to 15.6 micrograms/milliliter; the obtained procyanidine is high in purity and good in activity, can be directly applied to medicines and health-care foods.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of proanthocyanidins, and in particular relates to a method for extracting and purifying proanthocyanidins by using Aronia nigra as a raw material. Background technique [0002] At present, the extraction methods of proanthocyanidins (referred to as OPC) mainly include the following: [0003] 1. Solvent method: This method is one of the most common methods in the extraction of proanthocyanidins, and it is also one of the earliest and most widely used methods. The solvents are commonly used in water, methanol, ethanol, acetone, and petroleum ether. Although the process is simple, it is polar. The extensive use of toxic solvents can easily cause problems in environmental pollution, production safety, and product safety. At the same time, high energy consumption has always been one of the outstanding problems in the application of this method. Chen Jian and others used methanol t...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCY02P20/54
Inventor 张佳霖汪长钢孙智谋周旭戚译天
Owner 吉林省轻工业设计研究院
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