Method for extracting proanthocyanidins from Yunnan pine bark

A technology of proanthocyanidins and pine bark, which is applied in the direction of organic chemistry, can solve the problems of high toxicity and side effects, low extraction rate of products, and simultaneous enzymatic hydrolysis, etc., and achieves the effects of convenient operation, simple process, and reduced energy consumption and operating costs

Inactive Publication Date: 2017-05-10
田珩
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The principle of this method is simple, and the requirements for equipment are low. The disadvantage is that most organic solvents have high toxicity and side effects and the extraction rate of products is low.
In this method, the high-voltage pulsed electric field treatment cannot be processed simultaneously with

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Weigh 100 g of 60-mesh crushed Yunnan pine bark raw material, and put it into the pre-prepared cellulase and acetic acid-sodium acetate buffer solution with a pH of 4.8 at a weight ratio of 1:10. The enzyme activity of the cellulase used is 0.2-1.0 U / mg, and the weight is 0.3% of the bark raw material of Yunnan pine. The temperature of the solution is controlled at 35-38°C, and the stirring is continued for 40 minutes, supplemented by ultrasonic intensity of 6-12W / cm 2 , ultrasonic frequency range of 2MHz.

[0044] After 40 minutes of enzymatic hydrolysis, add 95% ethanol solution to the mixed solution according to 35% of the volume of the acetic acid-sodium acetate buffer solution, raise the temperature to 70°C, and supplement with ultrasonic intensity of 6-12W / cm 2 , ultrasonic frequency range of 2MHz, stirring continuously for 15 minutes.

[0045] Finally, the extract is subjected to traditional separation and purification. The extract is centrifuged and separated...

Embodiment 2

[0054] The pH value of the acetic acid-sodium acetate buffer solution was adjusted to 3.8 instead of 4.8, and the preparation steps of Example 1 were repeated. 3.4 g of the finished product was obtained, and the proanthocyanidin content was 75.3%.

Embodiment 3

[0056] The temperature of the enzymolysis solution was adjusted to 50°C instead of 35-38°C, and the preparation steps of Example 1 were repeated. 3.3 g of the finished product was obtained, and the proanthocyanidin content was 72.5%.

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Abstract

The invention discloses a method for extracting proanthocyanidins from Yunnan pine bark. The method comprises the following steps: (1) crushing Yunnan pine bark, and then adding cellulase and acetic acid-sodium acetate buffer solution to carry out enzymolysis on the crushed bark; (2) carrying out ultrasonic treatment in an enzymolysis process; (3) adding an ethanol solution into the enzymatic hydrolysis suspension, uniformly mixing, and carrying out ultrasonic treatment again; (4) separating and purifying the crude extract to obtain the proanthocyanidins extract. The extraction method is simple in process and convenient to operate, the yield of proanthocyanidins is increased by 15%-25% higher than that of a common solvent extraction method, and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for separating and extracting proanthocyanidins from the bark of Yunnan pine. Background technique [0002] Proanthocyanidins are polyphenolic compounds formed by the condensation of flavan-3-ol monomers and their polymers, usually composed of two types of proanthocyanidin monomers, catechin and epicatechin. Proanthocyanidins are hydroxyl donors, and their main function in plant tissues is to protect easily oxidized components in plants. In the 1980s, people gradually realized that scavenging free radicals and anti-oxidation are important prerequisites and foundations for nutrition and health care. Proanthocyanidins, as the antioxidant with the strongest ability to scavenge free radicals, are unmatched by other antioxidants. [0003] Antioxidant properties are mainly reflected in the following aspects: [0004] 1. Effectively remove supe...

Claims

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Application Information

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IPC IPC(8): C07D311/62C07G99/00
CPCC07D311/62C07G99/00
Inventor 田珩
Owner 田珩
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