43 results about "Flavan" patented technology

The invention discloses recombinant saccharomyces cerevisiae, a 11-seed oil fermentation product as well as a preparation method and application of the 11-seed oil fermentation product, and belongs to the technical field of synthetic biology and fermentation product preparation. According to the recombinant saccharomyces cerevisiae disclosed by the invention, a caffeic acid coenzyme A ligase 4CL coding gene, a chalcone isomerase CHI coding gene, a flavanone-3-hydroxylase F3H coding gene and a chalcone synthase CHS mutant coding gene are over-expressed, and the chalcone synthase CHS mutant is preferably Y69H / H71Y / Q161K. The preparation method of the 11-seed oil fermentation product comprises the following steps: fermenting a recombinant saccharomyces cerevisiae fermentation solution in the presence of a sophora flower bud extract and caffeic acid to obtain a first fermentation solution; fermenting the lactic acid bacteria fermentation liquor in the presence of 11 seed oil to obtain second fermentation liquor; and mixing the first fermentation liquid and the second fermentation liquid, continuing fermentation, and respectively collecting an oil phase and a water phase after fermentation is finished, so as to obtain a fermentation product. The product provided by the invention has multivitamin antioxidant activity.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a glycyrrhiza uralensis n-butyl alcohol extract and application of effective components thereof. The invention relates to a glycyrrhiza uralensis n-butyl alcohol extract. The effective components of the glycyrrhiza uralensis n-butyl alcohol extract are prepared from glycyrrhiza uralensis isoflavanone, glycyrrhiza stem alcohol A, licorcidine, dibutyl phthalate, glycyrrhizin I, 3 '-Dimethylallylkievitone, lupinone, glycyrrhiza flavonol, edediol, 1-methoxy phaseolin and isoderrone. The glycyrrhiza uralensis n-butyl alcohol extract is prepared from the following raw materials: glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis, glycyrrhiza uralensis and glycyrrhiza uralensis, the anti-streptococcus mutans activity of the glycyrrhiza uralensis n-butyl alcohol extract provided by the invention is superior to that of an extract extracted by ethanol in the same extraction method. The method has more advantages in the aspect of preventing streptococcus mutans infection and decayed teeth, and a theoretical basis is provided for research and development of new products.

This invention discloses 2'-hydroxy-4,4',6'-trimethoxychalcone and 5,7,4'-trimethoxyflavanone sulfonamide derivatives, their synthesis methods and applications, belonging to the field of pharmaceutical technology, and involving two series of compounds: 3-sulfonamido-2'-hydroxy-4,4',6'-trimethoxychalcone and 8,3'-disulfonamido-5,7,4'-trimethoxyflavanone, and their preparation methods. Starting with 2-hydroxy-4,6-dimethoxyacetophenone and p-anisaldehyde or 4-methoxy-3-nitrobenzaldehyde, 2'-hydroxy-4,4',6'-trimethoxychalcone or 3-amino-2'-hydroxy-4,4',6'-trimethoxychalcone intermediates were synthesized via the Claisen-Schmidt reaction. Then, through sulfonation and intramolecular Michael addition reactions, 2'-hydroxy-4,4',6'-trimethoxychalcone and 5,7,4'-trimethoxyflavanone sulfonamide derivatives were synthesized. The preparation method is simple, mild, and yields high results. In vitro and in vivo activity tests show that the derivatives disclosed in this invention have significant in vitro inhibitory activity against gastric cancer, esophageal cancer, colorectal cancer, and lung cancer cell lines, acting as inhibitors of the protein YBX1 / RPN1. Clinically, they can be applied to targeted therapy for cancers such as gastric cancer, esophageal cancer, colorectal cancer, and lung cancer.

A cycloheptenone adduct of flavanols and stilbene compounds as shown in formula (I), its preparation method, and its use in treating cerebral ischemia. The compound of this invention has a simple preparation method and exhibits good neuroprotective activity.

The invention belongs to the technical field of nano-drug carriers, and particularly relates to a method for encapsulating flavantriol with fullerol and application of the method. Fullerol is used as a carrier to entrap flavantriol, a nano-composite system is successfully constructed through a mild and environment-friendly ultrasonic-assisted process, and the encapsulation efficiency and stability of flavantriol are improved. The prepared flavantriol-entrapped solid product can significantly improve the bioavailability and biological activity of flavantriol, and has wide application prospects in preparation of drugs, cosmetics or functional foods for resisting inflammation, resisting oxidation and relieving chemotherapy side effects.

Present invention discloses a novel green fluorescence silver nanodots prepared with naringenin, a flavanone, by a near green synthesis without adding harsh reducing agent such as sodium borohydride and others. Particularly, the present invention provides silver nanodots (quantum dot like nanostructure) of average size 3.5 nm. The preparation method is a unique kind of single pot green synthesis method wherein no common and strong reagent like sodium borohydride, ascorbic acid surfactant, thiol containing reagent or protein solutions are used. Alternatively, a simple flavanone, aromatic organic molecule that contains three OH groups is used both as a mild reducing and stabilizing agent for the preparation of the particles at room temperature (25°C). The aqueous suspension of the invented nanodots product is quite stable in room temperature. It produces strong green fluorescence with average fluorescence lifetime ~2.37 ns and produces a large stocks shift.

The invention relates to application of flavanone as a bactericidal synergist in plant disease control. Specifically, the bactericidal synergist, a substituted aniline bactericide, a methoxy acrylate bactericide, a triazole bactericide and a substituted benzene bactericide are jointly applied to prevention and treatment of diseases. Flavanone has a synergistic effect on the bactericide, and can enhance the toxicity of the bactericide, improve the sensitivity of a drug-resistant strain to the bactericide and promote the control effect of the bactericide on indoor or field drug-resistant phytopathogens when being mixed with the bactericide, and can effectively reduce the dosage of the bactericide. And a new method is provided for the development and application of the existing new bactericide preparation.

A composition for forming a protective film for a wet etching solution for semiconductors, comprising: component (A): a film-forming component; component (B): a compound selected from the group consisting of a condensed tannin (b1) obtained by polymerizing a compound having a flavanol skeleton, a hydrolyzable tannin (b2) obtained by ester-bonding a sugar and an aromatic compound of gallic acid or ellagic acid, and a flavonoid (b3) derived from a compound having a flavanone skeleton structure; and component (C): a solvent.

The invention belongs to the technical field of bioengineering, and particularly relates to an AbF3H enzyme and application thereof, it is found through bioinformatics analysis that the enzyme belongs to an alpha-ketoglutaric acid dependent dioxygenase family and has the potential of catalytic synthesis of dihydrokaempferol, and the amino acid sequence of the enzyme is shown as SEQ ID No.1. Escherichia coli BL21 (DE3) is used as host bacteria, plasmid pACYCDuet-1 is used for constructing an expression system of the enzyme, and a recombinant strain can be used for synthesizing dihydrokaempferol by taking naringenin as a substrate and supplementing vitamin C, ferrous sulfate and alpha-ketoglutaric acid through a whole-cell catalysis method. The preservation number of the recombinant strain is CCTCC (China Center For Type Culture Collection) M 20251244. The content of the invention enriches the resource library of flavanone 3-hydroxylase, and lays a foundation for industrial production of dihydrokaempferol and other flavonoid compounds.

The invention discloses a gingko GbF3H gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbF3H gene is obtained by analyzing and screening on the basis of ginkgo transcriptome data in the earlier stage of a research group, the GbF3H gene is flavanone 3-hydroxylase, the nucleotide sequence of the GbF3H gene is shown as SEQ ID NO.1, and the amino acid sequence of expression protein of the GbF3H gene is shown as SEQ ID NO.2. A GbF3H overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the synthesis of flavonoid substances in a transgenic plant is obviously changed, the difference of 28 flavonoid metabolites is obvious, and the contents of 9 flavonoid substances such as formononin are up-regulated. The invention provides a theoretical basis, a valuable gene resource and a method for analyzing a ginkgo flavone synthesis mechanism and improving the flavone yield through metabolic engineering, and has important significance on development and utilization of ginkgo resources and cultivation of new germplasm with medicinal value.

The application discloses an ionic liquid 7-galloyl-tetrol flavan molecular imprinting polymer, which is prepared by using 7-galloyl-tetrol flavan as a template molecule, using an ionic liquid as a functional monomer, using an acetonitrile:1-ethyl-3-methyl imidazole tetrafluoroborate mixed solution as a porogen, and through a bulk polymerization method, under the joint action of a crosslinking agent, an initiator and the porogen, the functional monomer is polymerized with the template molecule, so that the ionic liquid 7-galloyl-tetrol flavan molecular imprinting polymer is prepared. The 7-galloyl-tetrol flavan is used as the template molecule for the first time, and the green and environment-friendly imprinting polymer material is prepared in combination with the ionic liquid, the imprinting polymer material has obvious specific adsorption, has a wide application range, can still exhibit strong specific selectivity to the template molecule in an aqueous phase, and can efficiently separate and enrich effective components.

The invention belongs to the technical field of medicinal chemistry and pharmacology, and particularly relates to a flavanone thiazole derivative as well as a preparation method and application thereof. The structural formula of the flavanone thiazole derivative is shown in the specification, and in the structural formula, R represents one of 2-iodophenyl, 2, 4-dichlorophenyl, 4-fluorophenyl, 2-bromobenzyl, 4-bromophenyl, 2-bromophenyl, 4-chlorobenzyl and 4-methylphenyl. The flavanone thiazole derivative is a flavanone thiazole ring-fused derivative, and pharmacological research shows that the compound has high liver cancer cell proliferation inhibition activity and can be applied to preparation of drugs for preventing and treating liver cancer.

This invention discloses the application of cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins in the preparation of drugs for treating or preventing aging and lowering lipids. In vivo experiments have confirmed that the cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins of this invention, without reproductive toxicity to a *C. elegans* model, can prolong the average lifespan of nematodes, improve their motility, enhance their stress resistance, reduce endogenous ROS levels and lipofuscin content, and decrease the body fat content of nematodes. Therefore, the cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins of this invention can be used in the preparation of drugs for treating or preventing aging and lowering lipids.

The application discloses a kind of for anti-aging regulation blood fat blood sugar, the purification method of high-purity tea pigment of uric acid.It is with summer and autumn tea crude leaf, tea residue as raw material, by enzyme directed conversion, double-frequency ultrasonic subcritical water extraction, three-stage membrane separation, molecularly imprinted resin precision purification, the integrated innovative process of low-temperature freeze drying, prepare high-purity tea pigment with total purity ≥95%, the flavan-3-ols ≥35%, active retention rate ≥95%, realize the safety standard of no solvent residue, low caffeine, desalination and heavy metal removal.The high-purity tea pigment has four synergistic effects of strong antioxidant anti-aging, protecting blood vessels and improving circulation, regulating blood sugar, inhibiting uric acid generation and promoting excretion, and can be widely applied to functional food, health food, pharmaceutical intermediates and special medical food.The application has advanced process, green environmental protection, high raw material utilization rate and batch stability, solves the technical bottleneck of low purity, poor activity and insufficient safety of traditional tea pigment, and has significant innovation, practicality and industrialization value.

This invention discloses a method for preparing flavanone extracts through microbial co-fermentation and its application in lipid-lowering activity, belonging to the field of food nutrition and functional components. By co-fermenting orange peel raw materials with yeast and Monascus purpureus, the synergistic metabolic effects of different microorganisms are utilized to increase the content and reconstruct the composition of flavanones from orange peel. The bioactivity of the fermentation products was evaluated using a mouse 3T3-L1 preadipocyte differentiation model. The results showed that the flavanones from orange peel treated with co-fermentation had a significant inhibitory effect on lipid accumulation, and their lipid-lowering activity was significantly better than that of unfermented or single-strain fermentation products. The method of this invention is simple, reproducible, and suitable for large-scale production, and can be applied to the development of lipid-lowering functional health products or dietary supplements.

The invention discloses an application of a compound or a composition thereof as a semen cryopreservation solution. According to the invention, through whole genome re-sequencing and seminal plasma non-target metabonomics, a regulation mechanism of interaction of genome variation and metabolites on semen traits is analyzed. Key metabolites having a protection effect on sperm motility are screened from 3613 kinds of metabolites and 38 pieces of sperm character analysis of breeding bulls. The function is verified through a standardized freezing process, it is found that after the sperm concentration is adjusted to 6 * 10 < 8 > / mL, and incubation at 37 DEG C, gradient cooling and liquid nitrogen programmed freezing are carried out, it is found through detection that the effect of a treatment group added with 15 [mu] M of 4, 6-dihydroxyquinoline and 5 [mu] M of flavanone is the most remarkable, and the vitality of frozen sperms can be improved. The invention further finds that the 4, 6-dihydroxyquinoline and the flavanone are combined for use, so that a remarkable synergistic interaction effect can be generated. The method is simple and convenient to operate and easy to popularize, and provides a reliable means for cryopreservation of the semen.

To provide a polyphenol composition allowing specific polyphenols, being water-poorly-soluble compounds, to be dissolved at high concentration in a water-based solvent and enabling easy preparation of a highly transparent solution, and a method for producing the same.SOLUTION: The polyphenol composition of the present invention includes a water-poorly-soluble compound and a sucrose fatty acid ester, wherein the water-poorly-soluble compound is one polyphenol selected from the group consisting of flavones, flavanones, isoflavones, and flavonols, and the sucrose fatty acid ester has an average degree of esterification of 1.01 or more and 1.1 or less.SELECTED DRAWING: None

This application belongs to the field of food processing technology, and particularly relates to a preparation technology and application of fruit powder products with high citrus flavonoid content. The preparation technology of fruit powder products with high citrus flavonoid content provided by this application first extracts blood orange peel through supercritical CO2 combined with ethanol extraction. The obtained blood orange peel flavonoid extract contains rich polymethoxylated flavonoid active ingredients, flavanones, flavonols, and flavonoid active ingredients. After being added to fruit powder, the types of flavonoids and the total flavonoid content are greatly increased, and the antioxidant and other biological activities are improved, thereby solving the problem of the lack of deep processing technology for whole blood oranges in the prior art.

The invention belongs to the field of gene engineering, and relates to a gene combination for synthesizing delphinidin, a recombinant plasmid combination, a strain and a method for heterologous biosynthesis of delphinidin. The gene combination comprises the following genes: a flavanone 3-hydroxylase gene TbF3H, a P450 cytochrome oxidase gene TbCYP, a flavanonol 4-reductase TbDFR, an anthocyanin synthetase TbANS and a P450 cytochrome reductase gene LjCPR, and the flavanone 3-hydroxylase gene TbF3H, the P450 cytochrome oxidase gene TbCYP, the flavanonol 4-reductase TbDFR, the anthocyanin synthetase TbANS and the P450 cytochrome reductase gene The invention provides a potential method for replacing traditional artificial cultivation or submerged fermentation acquisition, and lays a foundation for further transforming efficient biosynthesis through metabolic engineering.

Various embodiments of the invention utilize flavanones, including, but not limited to, 3-hydroxyflavone compounds and 3-hydroxyflavone sugar compounds, to treat blood, bleeding, and / or bleeding disorders. As described herein, flavanones significantly reduce blood clotting time in normal / hemophilic blood and normal / hemophilic animal models. As also described herein, flavanones reduced blood clotting time and increased clotting efficiency in blood without any apparent risk of immunogenicity or risk of unwanted blood clots. As also described herein, flavanones reduce the inhibitory activity of antithrombin on thrombin-driven blood clotting, thereby increasing the effectiveness of the thrombin mechanism in clotting blood.

The invention relates to a method for constructing HPLC characteristic chromatograms of a Yumai decoction reference sample, two sets of HPLC methods are adopted to comprehensively characterize component characteristics of four medicines in a Yumai decoction prescription, the first set of HPLC characteristic chromatogram determines 10 characteristic peaks, and the first set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of prepared rehmannia root, and the second set of HPLC characteristic chromatogram belongs to six characteristic peaks of the medicine flavor of Yumai decoction. The components are respectively catalpol, adenosine, 5-hydroxymethylfurfural, rehmannia D, verbascoside and isoacteoside; the rhizoma anemarrhenae has three characteristic peaks, namely neomangiferin, mangiferin and isomangiferin; the first set of HPLC characteristic chromatogram determines five characteristic peaks which belong to beta-ecdysterone, the second set of HPLC characteristic chromatogram determines five characteristic peaks which belong to five characteristic peaks of radix ophiopogonis, and two peaks of the characteristic peaks are identified to be methylophiopogonanone A and methylophiopogonanone B respectively; the two sets of specific chromatogram methods established by the invention have good performance and better pertinence, can comprehensively characterize the component characteristics of the four medicines in the Yumai decoction prescription, and can effectively monitor the quality of the Yumai decoction preparation.

The invention belongs to the technical field of biological medicines, and particularly relates to application of a NOTCH1 inhibitor or a compound in resisting diabetic nephropathy. The NOTCH1 inhibitor is a reagent for inhibiting the expression of NOTCH1 or a reagent for inhibiting the expression of an ANK structural domain of NOTCH1; the compound is prepared from any one or more of 2 ', 4', 5, 7-tetrahydroxyflavanone, icariin A, cassythine, anonaine, tenuigenin, bergamotin, teasel root saponin VI, (+) magnoline and piperlongumine. The invention discloses a new mechanism that NOTCH1 is directly recruited through an intracellular structural domain of NOTCH1 and is combined with KEAP1 to promote proteasome-dependent NRF2 polyubiquitination degradation so as to regulate and control oxidative damage and ferroptosis of podocytes. Meanwhile, it is found that all the nine compounds can inhibit a NOTCH1 signal channel and have the potential of treating DKD through a targeted NOTCH1 / KEAP1 / NRF2 axis.

An electrolyte complex is derived from a reaction mixture comprising an anionic saccharide, or a pharmaceutically acceptable salt thereof; and a flavylium cation, a glycoside thereof, an acylated derivative thereof, a pharmaceutically acceptable salt thereof, or a combination thereof. The flavylium cation may be derived from polyphenol enriched plants including berries. Also disclosed are polyphenol-enriched berry extracts and methods of preparing them. In particular, the disclosed extracts and complexes may be used in the treatment and prevention of metabolic disorders such as obesity, insulin resistance, type (2) diabetes, and polycystic ovarian syndrome.

The application belongs to the technical field of bioengineering, and particularly relates to an AbF3H enzyme and application thereof. Bioinformatics analysis finds that the enzyme belongs to an alpha-ketoglutarate-dependent dioxygenase family, has the potential of catalyzing synthesis of dihydromorin, and an amino acid sequence thereof is shown as SEQ ID No.: 1. Escherichia coli BL21 (DE3) is used as a host bacterium, a plasmid pACYCDuet-1 is used to construct an expression system of the enzyme, the recombinant strain can use naringenin as a substrate, and dihydromorin is synthesized by using a whole cell catalysis method with the aid of vitamin C, ferrous sulfate and alpha-ketoglutarate. The preservation number of the recombinant strain is CCTCC M 20251244. The content of the application enriches a flavanone 3-hydroxylase resource library, and lays a foundation for industrialized production of dihydromorin and other flavonoid compounds.

To provide a bioflavonoid composition for establishment and regulation of homeostasis of a host defense mechanism.SOLUTION: A composition is provided that comprises at least one standardized bioflavonoid extract enriched for at least one free-B-ring flavonoid and at least one standardized bioflavonoid extract enriched for at least one flavan.SELECTED DRAWING: None

The application discloses a salt-tolerant endophytic Pantoea and application thereof, and relates to the technical field of microorganisms. Pantoea endophytica A salt-tolerant endophytic Pantoea (TH1) is isolated from grape young fruits, and the preservation number of the strain is CCTCC NO: M 2025960. The strain can promote grape growth, improve grape quality, increase the content of vanillin in the grape, increase the content of anthocyanin, flavonoids and flavanone in the grape, and reduce the content of tannin and total phenol in the grape. In addition, the strain has the abilities of high-efficiency potassium solution, nitrogen fixation, phosphorus dissolution and IAA production, and salt tolerance, can improve plant photosynthesis, improve soil environment and promote plant growth under salt stress, and can also be used for preventing and treating grape powdery mildew.

The invention provides a thin-layer identification method for radix ophiopogonis and rhizoma anemarrhenae in a Yuyu decoction reference sample and application thereof.The radix ophiopogonis and rhizoma anemarrhenae are identified through an established thin-layer chromatography method, and the method mainly comprises the steps that trichloromethane is adopted as an extraction solvent, methylophiopogonanone B is adopted as a reference substance, toluene-methanol (80: 3) is adopted as a developing solvent, and the thin-layer chromatography method is adopted to identify the radix ophiopogonis and rhizoma anemarrhenae; identifying the medicinal flavor of the radix ophiopogonis under the condition of 365nm ultraviolet light; 50% ethanol is used as an extraction solvent, a rhizoma anemarrhenae reference medicinal material, mangiferin and timosaponin BII are used as contrasts, an upper-layer solution of n-butyl alcohol-glacial acetic acid-water (4: 1: 5) is used as a developing solvent, 10% ethanol sulfate is used as a color developing agent, and the medicine flavor of rhizoma anemarrhenae is identified under the condition of 365nm ultraviolet light. The thin-layer chromatography identification method developed by the invention not only has a visual and vivid identification result, but also has good specificity, durability and reproducibility, and perfects a quality control system of the Yumai decoction preparation.

The invention discloses an application of a soybean flavanone-3-hydroxylase gene and a soybean flavanone-3-hydroxylase gene and a soybean flavanone-3-hydroxylase gene. The nucleotide sequences of the soybean GmF3H1, the soybean GmF3H2 and the soybean GmFNSII-1 are as follows: SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3 respectively. The CRISPR / Cas9 is used for simultaneously knocking out GmF3H1, GmF3H2 and GmFNSII-1 in the soybean, so that the expression level of the CRISPR / Cas9 in the soybean can be obviously reduced. Compared with a wild type, the hundred-grain weight, the branching number and the protein content of the three-gene mutant material are also stably increased in multiple generations. Therefore, the soybean flavanone-3-hydroxylase genes GmF3H1 and GmF3H2 and the flavone synthase gene GmFNSII-1 disclosed by the invention can be applied to the aspects of soybean hundred-grain weight, plant type improvement and high-protein variety quality improvement of soybean knockout strains through gene engineering regulation.

This invention relates to a polyphenolic compound extracted from *Pterocarya stenoptera* and its application in the preparation of drugs for inhibiting enteritis. The polyphenolic compound is the first to be isolated from *Pterocarya stenoptera*, and is a novel, stable, and naturally occurring flavan-3-ol dimer monomer, rather than a polymer degradation product. The preparation method can stably produce high-purity polyphenolic compounds, suitable for the large-scale preparation of novel active ingredients from natural products. Experiments have shown that the polyphenolic compound of this invention possesses intestinal barrier repair capabilities, can inhibit inflammatory factors in the intestinal environment, and is more effective than commonly used clinical drugs. Furthermore, it is naturally derived, highly safe, and has significant clinical application value.

The application belongs to the technical field of pharmaceutical chemistry and pharmacology, and particularly relates to a flavanone schmidt rearrangement derivative, a preparation method and application thereof. A structural formula of the flavanone schmidt rearrangement derivative is shown in the following formula: wherein R represents one of benzyl, propynyl, allyl, isopentenyl, methyl, ethyl, isopropyl and n-butyl. The flavanone schmidt rearrangement derivative is a rearrangement compound obtained by schmidt rearrangement reaction of flavanone, and a derivative obtained by further alkylation. Pharmacological research shows that the compound has strong liver cancer cell proliferation inhibition activity, and can be applied to preparation of a drug for preventing and treating liver cancer.