Preparation method of Rhodiola rosea proanthocyanidin

A technology for anthocyanidin and procyanidin, which is applied in chemical instruments and methods, natural dyes, azo dyes, etc., can solve the problems of difficult to reach conditions, easy to block resin, resin pollution, etc., and achieve easy industrial production, easy recycling, and easy effect of operation

Inactive Publication Date: 2011-11-02
HEBEI AGRICULTURAL UNIV.
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Problems solved by technology

[0003] The above several methods have the following defects: (1) before the macroporous resin is adsorbed, there is no impurity removal, so that the impurities are easy to block the resin, causing resin pollution, and the amount of proanthocyanidin adsorption decreases; (2) it is suitable for harmful substances such as concentrated hydrochloric acid and methanol , which is not conducive to the application of proanthocyanidins in food, medicine, and cosmetics; (3) some conditions are not easy to achieve, such as low temperature

Method used

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Embodiment Construction

[0013] (1) crush the rhizome of Rhodiola rosea, add it to a 50% ethanol solution with a pH value of 5 according to the ratio of material to liquid 1:25, extract at a constant temperature of 80°C for 2 hours, filter, and repeat three times;

[0014] (2) Combine the filtrates, concentrate by rotary evaporation, and evaporate the ethanol to obtain the aqueous solution of the primary proanthocyanidin extract, use 4 times the volume of 75% ethanol to remove sugar, then degrease with an equal volume of petroleum ether, and concentrate to obtain the crude proanthocyanidin extract aqueous solution;

[0015] (3) The aqueous solution of the crude extract of proanthocyanidins is extracted 4 times with 1.5 times the volume of ethyl acetate, and each extraction takes 25 minutes to obtain an organic phase and an aqueous phase, which are concentrated by rotary evaporation respectively. The organic phase obtains the crude product of oligomeric procyanidins, and the aqueous phase obtains high ...

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Abstract

The invention relates to a preparation method of Rhodiola rosea proanthocyanidin, comprising the following steps of: taking Rhodiola rosea rhizomes as a raw material, crushing the Rhodiola rosea rhizomes, sieving, carrying out a refluxing process for two hours by the use of ethanol at the concentration of 50% to obtain a proanthocyanidin crude extract; removing impurities from the crude extract, removing carbohydrates by high concentration ethanol, and removing lipid substances by petroleum ether; extracting by the use of ethyl acetate, separating high polymeric proanthocyanidin from oligomeric proanthocyanidin; finally purifying the high polymeric proanthocyanidin and the oligomeric proanthocyanidin by the use of a macroporous adsorption resin, followed by freeze-drying until the purity of the high polymeric proanthocyanidin and that of the oligomeric proanthocyanidin are respectively 95.0% and 88.3%. The method provided by the invention has advantages of simple technology and no pollution, and is easy to apply in industrial production; and the prepared proanthocyanidin has high purity and can be applied in natural pigment food additives as well as medical health-care raw materials which have an antioxidant function.

Description

Technical field: [0001] The invention relates to extracting proanthocyanidins from rhizomes of rhodiola rosea and a preparation method thereof, belonging to the field of food industry. technical background: [0002] Procyanidins (PC) is a general term for flavan-3-ol derivatives widely present in plants, which are polymerized from different amounts of catechin or epicatechin monomers. Studies have shown that proanthocyanidins have strong antioxidant and free radical scavenging activities, prevent cardiovascular diseases, anti-tumor, lower blood pressure, anti-aging, etc., and have been widely used in food, medicine, cosmetics and other fields. In the prior art, the Chinese patent application No. 200410053240 discloses a patent document named "a method for preparing proanthocyanidins with high content". High content of proanthocyanidins was obtained through analysis, separation and solvent precipitation. The proanthocyanidin content of the solvent crude extract is about 50%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00
Inventor 赵文殷志萍孟玉彩
Owner HEBEI AGRICULTURAL UNIV.
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