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Method for extracting grape seed oil and proanthocyanidin

A technology for grape seed oil and proanthocyanidins, which is applied in the field of extracting grape seed oil and proanthocyanidins, can solve the problems of low product purity, poor membrane filtration selectivity, large solvent consumption, etc., and achieves a high degree of industrialization, short production cycle, and low solvent capacity. Effect

Inactive Publication Date: 2013-01-02
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent also uses atmospheric pressure and high temperature extraction, the amount of solvent used is large, and membrane filtration has poor selectivity and low product purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 1 kg of grape seeds, crush them, put them into a subcritical extraction kettle, feed propane, and extract dynamically for 30 minutes at a temperature of 40°C and a pressure of 4 MPa. The obtained extracts are recovered as reagents, and then 10 g of activated clay and activated carbon are added for decolorization and deodorization to obtain grape seeds Oil 120g. Pass water into the residue, and extract dynamically for 40 minutes at a temperature of 140°C and a pressure of 0.5 MPa. The obtained extract is cooled to room temperature and added to a HZ818 macroporous resin column for adsorption. The impurities are eluted with 3 column volumes of 50% ethanol solution, and then used for 4 The column volume was 80% ethanol to elute the active ingredient, the eluent was concentrated, and spray-dried to obtain 40 g of proanthocyanidins. Content 95%.

Embodiment 2

[0017] Take 1 kg of grape seeds, crush them, put them into a subcritical extraction kettle, pass in butane, and extract dynamically for 50 minutes at a temperature of 60°C and a pressure of 4 MPa. The obtained extracts are recovered as reagents, and then 20 g of activated clay and activated carbon are added for decolorization and deodorization to obtain grapes. Seed oil 109g. Pass 70% ethanol into the residue, and extract dynamically for 40 minutes at a temperature of 120°C and a pressure of 1 MPa. Reagents are recovered from the obtained extract, which is added to a NK-II macroporous resin column for adsorption, and ethanol gradient elution is 30% by volume of 4 columns Impurities were eluted with ethanol solution, and the active ingredient was eluted with 5 column volumes of 70% ethanol. The eluate was concentrated and spray-dried to obtain 36 g of proanthocyanidins. The content is 98%.

Embodiment 3

[0019] Take 1 kg of grape seeds, crush them, put them into a subcritical extraction kettle, pass them into dimethyl ether, and extract them dynamically for 30 minutes at a temperature of 50°C and a pressure of 2 MPa, and then add 15 g of activated clay and activated carbon for decolorization and deodorization to obtain Grape seed oil 112g. Add ethyl acetate to the residue, and extract dynamically for 40 minutes at a temperature of 90°C and a pressure of 0.3 MPa. The obtained extract is then diluted with an appropriate amount of water and added to an ADS-21 macroporous resin column for adsorption. The volume of 4 columns is 20% Impurities were eluted with ethanol solution, and the active ingredient was eluted with 4 column volumes of 75% ethanol. The eluent was concentrated and spray-dried to obtain 34 g of proanthocyanidins. The content is 92%.

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Abstract

The invention discloses a method for efficiently and quickly extracting grape seed oil and proanthocyanidin, which comprises the following steps: 1) pulverizing grape seed, adding the pulverized grape seed into a subcritical extraction kettle, introducing a nonpolar organic reagent, dynamically extracting at 20-80 DEG C under the pressure of 0.3-5 MPa for 20-80 minutes, recycling the reagent from the extract, and adding atlapulgite and activated carbon for decolorization and deodorization, thereby obtaining the grape seed oil; and 2) introducing a polar organic reagent or water into the extraction residue, dynamically extracting at 20-150 DEG C under the pressure of 0.3-8 MPa for 30-90 minutes, recycling the reagent from the extract, adding into a macroporous resin column for adsorption, carrying out gradient elution with an ethanol solution, concentrating the eluate, and carrying out spray drying to obtain the proanthocyanidin. The method disclosed by the invention has the advantages of low energy consumption, low solvent volume and high product yield, and is suitable for industrialized popularization.

Description

technical field [0001] The invention relates to a method for extracting grape seed oil and proanthocyanidins, in particular to a method for extracting grape seed oil and proanthocyanidins by using a subcritical fluid. Background technique [0002] Grape seed is the seed of grapes. It is rich in various oils, polyphenols, vitamins and minerals, etc. It has super antioxidant effect and is widely used in beauty and health care. Among them, studies have found that linoleic acid in grape seed oil can resist free radicals, anti-aging, help absorb vitamins C and E, strengthen the elasticity of the circulatory system, reduce UV damage, protect collagen in the skin, improve venous swelling and edema, Prevent melanin precipitation. Proanthocyanidin (OPC) in grape seed is an efficient free radical scavenger. Its anti-free radical oxidation function is 50 times that of vitamin E and 20 times that of vitamin C. It can remove toxic free radicals in the human body, protect human cells a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C07D311/62
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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