75 results about "Lycium ruthenicum" patented technology

The invention discloses a lycium ruthenicum biomass carbon dot as well as a preparation method and application thereof. The novel lycium ruthenicum carbon dots are prepared by taking lycium ruthenicum as a raw material and adopting a one-step hydrothermal method, and the novel lycium ruthenicum carbon dots are used for detecting residual herbicides in soil without being interfered by other substances. The method for detecting the herbicide residues in the soil by adopting the prepared novel carbon dots is superior to a large instrument, the detection method has the advantages of simplicity in operation, low cost, high sensitivity and the like, the source of the lycium ruthenicum is wide, and the preparation process is green and environment-friendly. Therefore, the prepared carbon dots provide a new method for detection of the fine phosphinate ammonium salt, and a thought and a method are provided for specific application in agricultural production.

The invention discloses a special diet and a preparation process thereof, and the special diet comprises the following raw materials: 500-600 parts of a red date concentrated solution, 350-400 parts of purified water, 40-60 parts of collagen peptide, 4-6 parts of lycium ruthenicum primary pulp, 0.5-1.5 parts of donkey-hide gelatin oligopeptide powder, 0.5-1.5 parts of longan powder, 3-5 parts of gamma-aminobutyric acid essence, 0.5-1.5 parts of elastin peptide, 0.5-1.5 parts of cubilose peptide, and 0.5-1.5 parts of collagen tripeptide. And 0.5 to 1.5 parts of epigallocatechin gallate. According to the special diet disclosed by the invention, the matching of the red date concentrated solution and the lycium ruthenicum primary pulp not only provides abundant natural antioxidant substances, but also enhances the blood replenishing and beautifying functions of the product, and the combination of the collagen peptide, the elastin peptide and the collagen tripeptide provides multi-level nourishing protection and the like for skin and joint health. The whole preparation process is accurate and controllable in technological parameters, high in production efficiency and suitable for large-scale production, and the obtained product has the remarkable characteristics of being fine and smooth in taste, definite in effect and good in stability and can meet the diversified requirements of different people for functional dietary supplements.

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses an anti-aging and anti-fatigue traditional Chinese medicine formula and a preparation method thereof. The traditional Chinese medicine formula comprises the following raw materials in parts by weight: 10-12 parts of ginseng, 10-15 parts of rhodiola rosea, 8-10 parts of dendrobium officinale, 5-6 parts of turmeric, 5-8 parts of cordyceps militaris, 8-10 parts of radix puerariae, 6-10 parts of schisandra chinensis, 1-3 parts of liquorice, 8-10 parts of lycium ruthenicum, 8-10 parts of cistanche, 10-12 parts of acanthopanax and 8-10 parts of phellinus igniarius. The adaptive capacity of an organism to an anoxic environment is improved, fatigue is effectively relieved, and various active ingredients contained in the health-care food contribute to delaying the senescence process.

The invention discloses application of lycium ruthenicum polysaccharide and a medicine or health food for improving sleep, the lycium ruthenicum polysaccharide is used as a product for improving sleep, and the lycium ruthenicum polysaccharide has no tolerance while improving sleep and has high safety. Besides, as a natural traditional Chinese medicine extract, lycium ruthenicum polysaccharide is safe and green in source, small in side effect, capable of regulating the body in multiple aspects and suitable for long-term administration, has the prospect of being developed into products for improving sleep, provides more choices for improving insomnia clinically, and has important social benefits and economic values.

The invention discloses a female-breeding primary pulp composition based on a seed circulation method and a preparation method and application thereof, and relates to the technical field of functional foods.The female-breeding primary pulp composition is prepared from, by weight, 15%-25% of organic flaxseeds, 15%-25% of organic black beans, 10%-20% of figs, 10%-20% of soybeans, 5%-15% of the root of kudzu vine, 5%-15% of pumpkin seeds and 2%-8% of lycium ruthenicum. The invention also provides a preparation method of the female-nourishing protoplasm composition and application of the female-nourishing protoplasm composition in functional food for regulating the balance of female estrogen and progestational hormone. According to the female-nourishing puree composition based on the seed circulation method, various medicinal and edible raw materials are selected and scientifically compounded, a special processing technology is adopted for wall breaking and effect releasing, and the ready-to-drink puree which can be drunk fixedly every day so as to realize synchronous balance adjustment of estrogen and progesterone is prepared; the pain points of high cycle dependence, low absorption rate and inconvenience in use of the traditional method are fundamentally solved.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition and application of the traditional Chinese medicine composition in preparation of drugs for improving sleep disorder and treating mood disorder or cognitive disorder. The invention provides a traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by mass: 20-30 parts of lycium ruthenicum, 30-40 parts of nitraria fruit, 15-25 parts of gastrodia elata, 13-25 parts of hyperforin perforatum and 2-6 parts of lycopene. The traditional Chinese medicine composition provided by the invention is prepared from lycium ruthenicum, nitraria fruit, gastrodia elata, hyperforin perforatum and lycopene according to a specific ratio, and the composition can effectively relieve anxiety behaviors of chronic stress bound anxiety model mice through ways of resisting oxidation and inflammation, regulating expression of neurotransmitters and the like, prolong sleep time and improve sleep quality. The learning ability and the memory ability of cognitive impairment mice induced by scopolamine are improved, and the neuron density of brain tissues is increased, so that the neuroprotective effect is achieved.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to an external traditional Chinese medicine ointment for treating acute gouty arthritis and a preparation method thereof. Comprising the following components in parts by mass: 18-22 parts of rheum officinale, 20-26 parts of turmeric, 15-19 parts of cortex phellodendri, 15-19 parts of indigo naturalis, 15-19 parts of rhizoma smilacis glabrae, 14-18 parts of radix clematidis, 14-18 parts of moutan bark, 14-18 parts of herba lycopi, 10-14 parts of semen plantaginis, 8-12 parts of radix stephaniae tetrandrae, 8-12 parts of rhizoma corydalis and 5-9 parts of liquorice. The ointment not only aims at inflammation and pain in the acute stage of gout, but also promotes uric acid excretion through medicines such as rhizoma smilacis glabrae and semen plantaginis, and has a certain auxiliary uric acid reduction effect. The compound design of the traditional Chinese medicine composition integrates the functions of clearing heat and promoting diuresis and promoting blood circulation to remove blood stasis, body metabolism is regulated from multiple targets, symptoms are relieved, pathogenesis conditioning is also considered, and a more comprehensive solution is provided for treatment of gouty arthritis.

The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by mass: 5 to 10 parts of cortex phellodendri, 5 to 10 parts of radix scutellariae, 5 to 10 parts of radix notoginseng, 5 to 10 parts of melastoma dodecandrum lour, 8 to 15 parts of herba lycopi, 8 to 15 parts of rhizoma alismatis, 8 to 15 parts of herba lycopodii, 12 to 18 parts of semen coicis, 1 to 3 parts of borneol, 135 to 150 parts of beewax and 390 to 400 parts of sesame oil. The She nationality characteristic medicinal material melastoma dodecandrum serves as a core component, the Qi and blood theory of traditional Chinese medicine is combined, multiple blood circulation promoting and stasis removing medicinal materials are combined, the comprehensive treatment effects of promoting blood circulation to remove swelling, regulating Qi to dissipate blood stasis and stopping bleeding and relieving pain are jointly achieved, the red, swollen and heat pain symptoms in the acute stage of fracture and sprain can be remarkably relieved, and the curative effect is good. The traditional Chinese medicine composition has the action trend of reducing local exudation and preventing swelling persistence, and has a practical clinical external treatment value for promoting early functional recovery of wounds.

The invention belongs to the field of biological medicine, and particularly relates to a traditional Chinese medicine composition for treating xerophthalmia and relieving asthenopia and a preparation method thereof, the traditional Chinese medicine composition comprises mulberry leaf, chrysanthemum, cassia seed, wolfberry, rehmannia, dogwood, moutan bark, Chinese yam, lily, poria cocos, rhizoma alismatis, dendrobe, Chinese angelica, marigold, blueberry, lycium ruthenicum, raspberry, lutein ester and bear gall gold powder. Through the scheme, the traditional Chinese medicine composition provided by the invention can effectively treat clinical symptoms of xerophthalmia, increase tear secretion, improve fundus microcirculation and increase ciliary muscle blood flow, thereby eliminating asthenopia symptoms and improving eyesight.

The invention relates to an application of a traditional Chinese medicine composition in preparation of food, medicine or health food with an effect of delaying senescence, and the traditional Chinese medicine composition is prepared from the following raw material components in parts by weight: 10-35 parts of lycium ruthenicum, 3-10 parts of trollflower, 3-10 parts of liquorice, 2-9 parts of radix glehniae and 2-9 parts of lonicera confusa. The traditional Chinese medicine composition has the effects of improving physiological functions, resisting stress, preventing and treating neurodegenerative diseases and prolonging life, has the anti-aging effect proved from multiple aspects, and can be used for preparing medicines, health-care foods or foods with the effect of delaying aging. The traditional Chinese medicine composition disclosed by the invention can be prepared into a proper oral preparation together with pharmaceutically acceptable auxiliary materials according to needs to be used for assisting in delaying senescence treatment.

The invention belongs to the technical field of medicinal and edible products, and particularly relates to a composition for improving constipation and diarrhea as well as a preparation method and application thereof. The composition for improving constipation and diarrhea provided by the invention comprises the following raw materials in parts by weight: Chinese yam, resistant dextrin, ginseng, radix ophiopogonis, poria cocos, dogwood, semen coicis, aronia melanocarpa freeze-dried powder, mulberry, radix polygonati officinalis, lycium ruthenicum freeze-dried powder, fructo-oligosaccharide and crystalline fructose. Experiments prove that through synergistic cooperation of the raw materials, the composition can effectively improve the diarrhea index of diarrhea rats, improve the intestinal barrier function, increase the villus length of small intestine tissues, reduce the depth of crypts, increase the water content of excrement of constipation mice and promote intestinal peristalsis, and has the effect of bidirectionally regulating diarrhea and constipation; the intestinal barrier function can be favorably repaired.

This invention belongs to the field of plant active ingredient extraction technology, specifically relating to a process for efficiently extracting anthocyanins from black goji berries. The process includes the following steps: Step 1: Preparing black goji berry powder; Step 2: Adding buffer solution to the black goji berry powder to obtain a black goji berry suspension; adding a complex enzyme to the black goji berry suspension for enzymatic hydrolysis, enzyme inactivation, and cooling to obtain an enzymatic hydrolysate; Step 3: Adding Aspergillus niger to the enzymatic hydrolysate for fermentation, sterilization, and obtaining a primary fermentation broth; continuing to add a complex microbial culture for anaerobic fermentation, sterilization, and cooling to obtain a final fermentation broth; Step 4: Performing alcohol extraction on the final fermentation broth to remove ethanol, obtaining a concentrated crude extract; Step 5: Purifying the concentrated crude extract using AB-8 macroporous adsorption resin, drying, and obtaining the black goji berry extract. This invention's process can simultaneously improve the extraction rates of total alkaloids and anthocyanins from black goji berry extract.

The invention relates to the technical field of gene engineering, and provides a lycium ruthenicum MYB transcription factor LrMYB77, the amino acid sequence of which is as shown in SEQ ID NO.3. The invention also provides a gene LrMYB77 for coding a transcription factor, and a recombinant expression vector and host bacteria containing the gene. Meanwhile, the invention further provides application of the transcription factor or the gene in promoting synthesis of lycium ruthenicum anthocyanin and a specific method for increasing the synthesis amount of the lycium ruthenicum anthocyanin. According to the invention, the LrMYB77 gene with a regulation effect on anthocyanin synthesis is screened from lycium ruthenicum for the first time, and experiments verify that the expression or overexpression of the gene in a plant is regulated, so that the synthesis and accumulation of anthocyanin in the plant can be remarkably promoted, and the LrMYB77 gene has important significance on germplasm resource creation and molecular breeding of lycium ruthenicum rich in anthocyanin.

The invention relates to the field of functional beverages, and particularly discloses a lycium ruthenicum fermented beverage and a preparation method thereof. The lycium ruthenicum fermented beverage is prepared from the following raw materials in parts by weight: 18 to 25 parts of lycium ruthenicum primary pulp, 18 to 25 parts of red ginseng water, 0.2 to 0.8 part of lactic acid bacteria, 2 to 9 parts of erythritol and 0.02 to 0.08 part of donkey-hide gelatin peptide. In addition, the preparation method has the advantage of further improving the oxidation resistance of the lycium ruthenicum fermented beverage.

The invention discloses a separation process of an alpha-glucosidase inhibitor Lycbarbarspermides Q with a new structure in red Chinese wolfberry fruits and application of the alpha-glucosidase inhibitor Lycbarbarspermides Q with the new structure in the red Chinese wolfberry fruits. The specific preparation process comprises the following seven steps: extraction, on-line alpha-glucosidase inhibitor component identification, hydrophilic water pressure preparation column coarse separation, reversed-phase high-pressure preparation column preparation, hydrophilic high-pressure preparation column preparation, gel filtration chromatography and in-vitro alpha-glucosidase inhibition experiment. The method is low in cost, and the product purity is greater than 95%; the technical means adopted by the invention can be used for large-scale production: the raw material requirement is not high, the cost is low, and batch material preparation is easy; in the preparation process, solvents and separation materials used by an extraction solvent, a hydrophilic preparative column, a reversed-phase preparative column, gel chromatography and a reversed-phase chromatographic column can be recycled.

This invention relates to a genetic transformation method for Ningxia wolfberry using germinating seeds as explants, belonging to the field of plant genetic transformation technology. The invention mainly includes the following steps: (1) After disinfecting Ningxia wolfberry seeds, inoculate them onto a germination medium, and obtain germinating seeds through vernalization and dark culture treatment; (2) Activate and culture Agrobacterium rhizogenes and Agrobacterium tumefaciens strains carrying target gene expression frames to obtain a bacterial solution for infection; (3) Take the germinating seeds obtained in step (1) and mix them with the bacterial solution obtained in step (2) for infection, then transfer the infected germinating seeds to a co-culture medium or substrate for co-culture; (4) Cultivate the material after co-culture in step (3) to induce root growth, and detect the expression level of the target gene or reporter gene on the roots or plants to obtain transgenic positive plants. This invention has the advantages of simple operation, significantly shortened transformation cycle, and high transformation efficiency.

The invention discloses a traditional Chinese medicine fumigant for combustion type lung diseases and a preparation method thereof, and belongs to the field of important fumigants. The invention discloses a traditional Chinese medicine fumigant for combustion type lung diseases. Comprising the following raw materials in parts by weight: 5 parts of gastrodia elata, 5 parts of lucid ganoderma, 3 parts of bulbus fritillariae cirrhosae, 10 parts of desert cistanche, 5 parts of Chinese chive seeds, 10 parts of cynomorium songaricum, 10 parts of lycium ruthenicum, 5 parts of dendrobe, 10 parts of platycodon grandiflorum, 10 parts of pericarpium citri reticulatae, 10 parts of exocarpium citri grandis, 10 parts of poria cocos, 20 parts of dandelion, 10 parts of cucumber seeds, 5 parts of seaweed, 5 parts of angelica sinensis, 5 parts of cooked pseudo-ginseng, 10 parts of radix isatidis, 10 parts of folium mori, 5 parts of liquorice, 10 parts of cacumen platycladi and 10 parts of folia perillae acutae. And 150 parts of a fermentation broth.

1. The name of the design product: label (black wolfberry). 2. The use of the design product: the design product is used for product packaging labels. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view. 5. The design product is a thin product, and the left view, right view, top view and bottom view are omitted.

1. Name of the Design Product: Packaging Kit (Qiemagu Lyophilized Powder). 2. Use of the Design Product: The design product is used for product packaging. 3. Design Key Points of the Design Product: The combination of shape, pattern and color. 4. Picture or Photo Best Showing the Design Key Points: Perspective View 1 of Kit 1. 5. The claimed design includes color. 6. Kit 2 is a thin product. The left view, right view, top view and bottom view of Kit 2 are omitted as they are already shown in Perspective View 2 of Kit 2. The rear view of Kit 2 is omitted. 7. Other Situations Requiring Explanation - Kit Description: Kit 1 is the packaging box and Kit 2 is the inner packaging bag.

The invention relates to a saffron crocus eye pad and a method for controlling eye axis growth, and belongs to the technical field of eye care products. The eye patch comprises a patch carrier and a plant extraction gel layer coated on the patch carrier, the plant extract gel layer is composed of mulberry leaf extract, chrysanthemum extract, butterflybush flower extract, amur corktree extract, crocus sativus flower extract, lithospermum extract, madder extract, lycium ruthenicum extract, beet root juice powder, natural borneol, menthol, xanthan gum, purified water and other natural components. The crocus sativus flower extract can enhance the blood flow speed of choroid, thicken the choroid and effectively control the growth of an ocular axis, so that the effect of delaying or improving the development of myopia is achieved. According to the gel layer, a hydrogel structure is formed through xanthan gum, natural borneol and menthol are combined to provide cooling and soothing effects, and the gel layer has good skin affinity and use safety.

The invention discloses a lycium ruthenicum anthocyanin-containing composition for improving visual fatigue and wet macular degeneration as well as a preparation method and application of the lycium ruthenicum anthocyanin-containing composition, and belongs to the technical field of biological medicines. The composition is prepared from the following raw materials in parts by weight: 50 to 99 parts of lycium ruthenicum anthocyanin extract, 0.5 to 25 parts of lutein ester microcapsule powder and 0.5 to 25 parts of zeaxanthin microcapsule powder. According to the composition disclosed by the invention, the lycium ruthenicum anthocyanin extract, the lutein ester microcapsule powder and the zeaxanthin microcapsule powder are compounded, so that the effect of improving visual fatigue and the effect of improving wet macular degeneration can be improved.

The application discloses a method for separating active substances in Lycium ruthenicum Murr, and relates to the technical field of natural product separation, and comprises a chromatographic separation method for separating active substances norpetanin in Lycium ruthenicum Murr, wherein the active components in the methanol extract of Lycium ruthenicum Murr are separated by using a water / methanol / dichloromethane three-phase system for elution, further separated by gradient elution of a mobile phase of methanol / water, and finally subjected to gradient elution by using a mobile phase of water / acetonitrile, so that the target active substance is obtained in a retention time of 33-35 min. The active substance norpetanin is obtained for the first time by extracting active substances from Lycium ruthenicum Murr, the exact chemical structure of the active substance norpetanin is identified by nuclear magnetic resonance data, and it is found that the active substance norpetanin has anti-inflammatory activity, hypoglycemic effect and lipid-lowering effect.

The application discloses a new petunin derivative secopetanin in Lycium ruthenicum, an extraction method and application thereof, and a preparation chromatographic separation of Lycium ruthenicum extract, and the new petunin derivative secopetanin can be obtained through multiple preparation separations. The application further provides application of the new compound secopetanin in preparation of products for preventing and / or treating inflammation, in preparation of products for preventing and / or treating sugar metabolism disorder related diseases, and in preparation of products for preventing and / or treating lipid metabolism disorder related diseases.

The invention belongs to the technical field of traditional Chinese medicine. The invention relates to an anti-aging composition, in particular to an anti-aging composition and application thereof. The composition is prepared from red ginseng, lycium ruthenicum, dendrobium officinale, cordyceps militaris, poria cocos and radix rehmanniae recen according to a specific mass ratio. Researches show that the composition can significantly reduce the proportion of SA-beta-gal positive cells and down-regulate the expression level of senescence-related genes p21, and has a very good anti-senescence effect. Meanwhile, the composition can also remarkably promote the expression of anti-wrinkle genes, increase the synthesis amount of I-type collagen, improve skin elasticity and effectively inhibit melanogenesis, and has various synergistic effects of resisting aging, whitening and resisting wrinkles. In addition, an in-vitro cytotoxicity experiment shows that the composition is good in safety. Through scientific compatibility and accurate control of the proportion, common synergistic interaction of anti-aging, anti-wrinkle and whitening effects is realized, and a safe and efficient multifunctional composition is provided and has a wide application value.

The invention relates to the technical field of agricultural product quality evaluation, and particularly discloses a quality identification method of high-quality lycium ruthenicum and application thereof. The method comprises the following steps: detecting whether the lycium ruthenicum contains eight anthocyanin compounds mentioned in the application or not; the identification method comprises the following steps: S1, pretreating lycium ruthenicum; s2, separating and detecting the extract in the lycium ruthenicum; s3, qualitative analysis of anthocyanin compounds in lycium ruthenicum; s4, quantitative analysis of the anthocyanin compounds in the lycium ruthenicum. The eight anthocyanin compounds can be used for evaluating the quality of the lycium ruthenicum raw material, and have the advantages of guaranteeing the product quality and standardizing the market order; in addition, the identification method has the advantages of being accurate, rapid and comprehensive.

The invention provides ginseng paste capable of resisting oxidation and improving qi and blood, a ginseng compound extracting solution and a preparation method. The ginseng compound extracting solution is obtained by extracting a decoction piece set; the decoction piece set comprises longan aril, honey, ginseng, radix polygonati officinalis, mulberry, Chinese date, donkey-hide gelatin, black-bone chicken freeze-dried powder, saussurea involucrata culture dry product, lycium ruthenicum, black adenosine, sorbus pohuashanensis, paeonia ostii, seville orange flower, tea flower, 50-200 parts of double-petal red rose, roselle, spina date seed, lily, poria cocos, coix seed, phaseolus calcaratus, white hyacinth bean, 50-200 parts of lotus seed and liquorice. The FTIR infrared spectrum of the ginseng compound extracting solution has a-COOH characteristic absorption peak at 1713.21 cm <-1 >, and the ginseng compound extracting solution has a supramolecular structure. The ginseng compound extracting solution and the ginseng paste provided by the invention have the effects of resisting oxidation and improving qi and blood.

The invention discloses a medicinal and edible composition with an effect of reducing uric acid and a preparation method and application of the medicinal and edible composition. The medicinal and edible composition comprises the following components in parts by weight: 30-40 parts of lycium ruthenicum anthocyanin extract freeze-dried powder, 21-30 parts of galangal extract, 21-40 parts of gardenia extract and 9-15 parts of American ginseng extract. Comprising the following raw materials in parts by weight: 40 parts of lycium ruthenicum anthocyanin extract freeze-dried powder, 30 parts of galangal extract, 21 parts of gardenia extract and 9 parts of American ginseng extract, and the raw material extracts are uniformly mixed to obtain a raw material mixture. According to the invention, lycium ruthenicum, galangal, cape jasmine and American ginseng are scientifically compounded, so that the medicinal and edible composition with the effect of reducing uric acid is provided, the medicinal and edible composition has the characteristics of simple formula, obvious curative effect and no toxic or side effect, and the American ginseng has a harmonizing effect, is mild in formula and is used as an assistant. The whole formula is in synergistic compatibility, tonifies the liver and kidney, nourishes yin, smoothens joints, and jointly achieves the effects of reducing uric acid and relieving gout symptoms.

The utility model relates to the technical field of freeze-dried powder production equipment, and particularly discloses a preparation device of lycium ruthenicum anthocyanin freeze-dried powder. Comprising a reaction tank, a tank cover is arranged at an opening in the upper end of the reaction tank, a pressure-bearing reaction cavity is formed in the reaction tank, a porous filter cartridge is coaxially arranged in the reaction cavity, and the inner space is divided into an extraction cavity and a precipitation liquid outlet cavity through the filter cartridge. Through the arrangement of the stirring and crushing mechanism, high-frequency shearing and crushing can be implemented through the stirring and crushing mechanism in the crushing and extracting stage, trapezoidal crushing teeth mechanically stir and cut materials at a set rotating speed, and the materials are synchronously matched with an ultrasonic vibrator to generate a cavitation effect; the synergistic effect of physical wall breaking and chemical dissolution of cell walls of lycium ruthenicum materials in the extraction cavity is achieved, and the release efficiency of anthocyanin is effectively improved. The problems that a traditional preparation device is long in extraction period, low in effective component release efficiency and prone to causing process breakpoints are solved.

The application discloses a method for constructing a genetically engineered Kluyveromyces lactis strain for producing miR162a, which comprises co-transferring two plasmids expressing DCL1, HYL1 and SE genes from Arabidopsis thaliana into a Kluyveromyces lactis GG799 strain by electroporation, so that the yeast obtains the ability to cut plant-derived precursor miRNA, one of the plasmids synchronously expresses a precursor of miR162a from Lycium ruthenicum Murr, and the genetically engineered Kluyveromyces lactis strain for stably producing miR162a is obtained after acetamide screening. The application further discloses the genetically engineered Kluyveromyces lactis strain constructed according to the above method and application thereof. The genetically engineered strain constructed by the method has a high yield of miR162a at a laboratory flask level, and can stably produce miR162a after continuous subculture, the genetically engineered Kluyveromyces lactis strain exhibits the ability to promote osteogenic differentiation in a cell experiment, and can effectively improve the osteoporosis symptoms of mice in an animal experiment.

This invention relates to the field of genetic engineering technology, and in particular to the application and method of knocking out the LrTPP3 gene in suppressing the occurrence of thorns on branches of black wolfberry. This invention provides the application of knocking out the LrTPP3 gene in suppressing the occurrence of thorns on branches of black wolfberry, and the suppression of thorn occurrence after knocking out the LrTPP3 gene.