7 results about "Lycium ruthenicum" patented technology

This invention belongs to the field of plant active ingredient extraction technology, specifically relating to a process for efficiently extracting anthocyanins from black goji berries. The process includes the following steps: Step 1: Preparing black goji berry powder; Step 2: Adding buffer solution to the black goji berry powder to obtain a black goji berry suspension; adding a complex enzyme to the black goji berry suspension for enzymatic hydrolysis, enzyme inactivation, and cooling to obtain an enzymatic hydrolysate; Step 3: Adding Aspergillus niger to the enzymatic hydrolysate for fermentation, sterilization, and obtaining a primary fermentation broth; continuing to add a complex microbial culture for anaerobic fermentation, sterilization, and cooling to obtain a final fermentation broth; Step 4: Performing alcohol extraction on the final fermentation broth to remove ethanol, obtaining a concentrated crude extract; Step 5: Purifying the concentrated crude extract using AB-8 macroporous adsorption resin, drying, and obtaining the black goji berry extract. This invention's process can simultaneously improve the extraction rates of total alkaloids and anthocyanins from black goji berry extract.

This invention relates to a genetic transformation method for Ningxia wolfberry using germinating seeds as explants, belonging to the field of plant genetic transformation technology. The invention mainly includes the following steps: (1) After disinfecting Ningxia wolfberry seeds, inoculate them onto a germination medium, and obtain germinating seeds through vernalization and dark culture treatment; (2) Activate and culture Agrobacterium rhizogenes and Agrobacterium tumefaciens strains carrying target gene expression frames to obtain a bacterial solution for infection; (3) Take the germinating seeds obtained in step (1) and mix them with the bacterial solution obtained in step (2) for infection, then transfer the infected germinating seeds to a co-culture medium or substrate for co-culture; (4) Cultivate the material after co-culture in step (3) to induce root growth, and detect the expression level of the target gene or reporter gene on the roots or plants to obtain transgenic positive plants. This invention has the advantages of simple operation, significantly shortened transformation cycle, and high transformation efficiency.

The application discloses a method for constructing a genetically engineered Kluyveromyces lactis strain for producing miR162a, which comprises co-transferring two plasmids expressing DCL1, HYL1 and SE genes from Arabidopsis thaliana into a Kluyveromyces lactis GG799 strain by electroporation, so that the yeast obtains the ability to cut plant-derived precursor miRNA, one of the plasmids synchronously expresses a precursor of miR162a from Lycium ruthenicum Murr, and the genetically engineered Kluyveromyces lactis strain for stably producing miR162a is obtained after acetamide screening. The application further discloses the genetically engineered Kluyveromyces lactis strain constructed according to the above method and application thereof. The genetically engineered strain constructed by the method has a high yield of miR162a at a laboratory flask level, and can stably produce miR162a after continuous subculture, the genetically engineered Kluyveromyces lactis strain exhibits the ability to promote osteogenic differentiation in a cell experiment, and can effectively improve the osteoporosis symptoms of mice in an animal experiment.

This invention relates to the field of genetic engineering technology, and in particular to the application and method of knocking out the LrTPP3 gene in suppressing the occurrence of thorns on branches of black wolfberry. This invention provides the application of knocking out the LrTPP3 gene in suppressing the occurrence of thorns on branches of black wolfberry, and the suppression of thorn occurrence after knocking out the LrTPP3 gene.

This invention relates to the fields of biomedicine and functional food technology, and discloses an intestinal immune regulation system based on the microenvironmental release of black goji berry polysaccharides (LBPs). The system includes a microenvironment-responsive delivery unit for delivering and releasing LBPs. The delivery unit comprises a LBP core and a responsive layer encapsulating the core. The responsive layer contains at least one responsive material capable of responding to microenvironmental signals at the intestinal target site, enabling the delivery unit to undergo structural changes in response to these signals upon reaching the intestinal target site, thereby achieving the targeted release of the LBPs. This intestinal immune regulation system based on the microenvironmental release of LBPs constructs a physical barrier by encapsulating the active core of LBPs with a "responsive layer" composed of responsive materials. This system can effectively resist the damage to the LBP structure caused by the harsh environment of the upper digestive tract.

This invention belongs to the field of goji berry processing technology, specifically relating to a method, application, and processed products of black goji berries that utilize citric acid to improve the stability of anthocyanins in black goji berries. The method involves adding citric acid (2-O-β-D-glucosyl-L-ascorbic acid, AA-2βG) to black goji berry anthocyanins, utilizing intramolecular or intermolecular co-coloring interactions to inhibit anthocyanin degradation and color deterioration of the product. Compared to existing technologies, this represents a leap from "temporary color protection" to "relatively long-lasting stability." It is effective in complex systems: equally effective in black goji berry juice (a real food matrix containing various components such as sugars, acids, and phenols), and even more so. Citric acid (AA-2βG) is naturally found in goji berries and other solanaceous plants, and there are no safety concerns regarding its use as a food additive.

The utility model provides a kind of black medlar fresh fruit screening conveyor, including rack, conveying mesh belt, hot blast device, shaking driving device and be installed on the inclined air deflector assembly of the rack, the conveying mesh belt is installed on the rack, the hot blast device is arranged immediately below the conveying mesh belt, for towards immediately above blowing hot air, the shaking driving device drives the upper belt body of the conveying mesh belt up and down shaking;The inclined air deflector assembly is located immediately above the conveying mesh belt, the inclined air deflector assembly is inclinedly arranged from the low side of the conveying mesh belt towards the high side of the other side of the conveying mesh belt.This black medlar fresh fruit screening conveyor has the advantage that leaves and sand can be screened more thoroughly based on the device of the preceding clause invented by the applicant.