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Method for rapidly propagating high-quality seedlings of Lycium ruthenicum Murr.

A high-quality technology of black fruit wolfberry is applied in the field of rapid propagation of high-quality seedlings of black fruit wolfberry to achieve the effects of protecting germplasm resources, protecting the ecological environment and meeting market demands

Inactive Publication Date: 2013-07-03
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Haoren Taben et al. (2005) used stems with axillary buds to differentiate rooting and callus-induced proliferation of adventitious buds on Lycium ruthenicum, but there is no report on the rapid propagation of high-quality Lycium barbarum seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The present invention has the following sequential steps:

[0013] a. First, choose the high-quality seeds of Lycium rutabaga and wash them with running water for 2-3 hours;

[0014] b. Disinfect the rinsed seeds with 75% alcohol for 1 min, and then rinse the seeds after alcohol disinfection with sterile water for 3 to 4 times;

[0015] c. Disinfect the seeds after rinsing with sterile water with 0.1% mercuric chloride for 5 minutes, and then rinse the seeds sterilized with mercuric chloride for 3 to 4 times with sterile water;

[0016] d. Finally, inoculate the sterilized seeds in MS medium to cultivate sterile seedlings, the culture temperature is 24°C, the light intensity is 1500 LX, and the light time is 24h / d;

[0017] e. After culturing for 1 month, cut off the stems of the sterile seedlings, cut them into 0.5cm long stem segments, and insert MS+KH under sterile conditions. 2 PO 4 The rapid propagation culture of sterile seedlings was continued in 13.5mg / L + N...

Embodiment 2

[0019] The method of Example 2 is the same as that of Example 1, the difference is that the culture temperature of the sterile seedlings in step d is 25°C, and the light intensity is 1800LX. In step e, the illumination time is 14h / d.

Embodiment 3

[0021] The method of Example 3 is the same as that of Example 1, except that the culture temperature of the sterile seedlings in step d is 26°C, and the light intensity is 2000LX. In step e, the illumination time is 16h / d.

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Abstract

The invention relates to a method for rapidly propagating high-quality seedlings of Lycium ruthenicum Murr.. The method includes the steps as follows: firstly, seeds are washed with running water, sterilized with 75% (vol) alcohol and washed with aseptic water for 3 to 4 times; then, the seeds are sterilized with 0.1% (mass) mercuric chloride, washed with sterilized water for 3 to 4 times, and then inoculated in an MS culture medium to cultivate aseptic seedlings; and after the aseptic seedlings are cultivated for one month, the stems of the aseptic seedlings are cut off, and then cut into stem segments with the lengths of 0.5 cm and inoculated into a culture medium containing MSKH2PO413.5mg / LNAA0.1mg / L for cultivation. The method has the advantages that all the sterile seedling can take root, the aseptic seedlings of Lycium ruthenicum Murr. can be rapidly propagated, the period is short, the growth of the seedling is not influenced by factors of seasons, climate, natural disasters and the like, and the industrial production can be achieved; and by means of the technology, a plurality of problems of low propagation capacity, lack in resource, agriculture chemical residues and the like of a medicinal plant can be solved.

Description

technical field [0001] The invention relates to a method for cultivating Lycium nigra, and more particularly to a method for rapid propagation of high-quality seedlings of Lycium nigra. Background technique [0002] black fruit wolfberry ( Lycium ruthenicum Murr.) is Solanaceae Lycium barbarum, which belongs to a kind of halophyte medicinal plants. It is mainly distributed in Ningxia, Shanxi, Gansu, Qinghai, Xinjiang, Tibet and other provinces in China. Goji berry has a wide range of uses, and its fruit contains a variety of essential amino acids and trace elements, which can improve human immunity. Anthocyanins can not only be used in medicine and food, but also be used as natural dyes in the textile industry. It is the wild plant with the highest content of proanthocyanidins so far. At the same time, Lycium nigra has many advantages such as drought resistance, cold resistance, salt and alkali resistance, strong root tillering and poor soil resistance, and is a good tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 柳福智
Owner GANSU AGRI UNIV
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