The invention discloses a production process of nano
saline-alkali
cellulase, and relates to the technical field of biological
fermentation and
saline-alkali
enzyme culture, and the production process comprises the following steps: culturing halorhodobryum broadcloth, carrying out culture and
enzyme activity induction on a strain, pre-cooling, circulating for 2-4 times at 1500 bar, circulating for two minutes each time to obtain crude
enzyme, and carrying out graded purification to obtain
saline-alkali
cellulase; then carrying out molecular
domestication with
thermal stability,
high activity and pH value tolerance to obtain a nano saline-alkali
cellulase strain; the obtained nano saline-alkali cellulase strain is subjected to enlarged culture to obtain
fermentation liquor, and the
fermentation liquor is concentrated and dried to obtain the nano saline-alkali cellulase, the enzyme activity number of the nano saline-alkali cellulase can reach 18000 u / g, the nano saline-alkali cellulase is verified to be remarkable in effect in
field tests, large-scale popularization and industrialization promotion can be achieved, and the nano saline-alkali cellulase has good application prospects. The method is not only suitable for saline-
alkali soil, but also suitable for desert soil, drought, low temperature and other adversity environments, and has wide stress-resistant potential.