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Method for directly observing salt contents of different tissues of halogeton glomeratus

A technology of saline grass and content, which is applied in the direction of measuring devices, analysis materials, and material analysis using wave/particle radiation, etc., can solve the problem of inability to directly observe the difference of salt content in different tissues and organs of halo grass, and achieve high reliability , the result is accurate

Inactive Publication Date: 2016-07-27
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to avoid the deficiencies of the prior art and provide a method for directly observing the salinity content of different tissues of Halophylla, so as to solve the difficult problem that it is impossible to visually observe the difference in the salinity content of different tissues and organs of Halophysis

Method used

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  • Method for directly observing salt contents of different tissues of halogeton glomeratus
  • Method for directly observing salt contents of different tissues of halogeton glomeratus
  • Method for directly observing salt contents of different tissues of halogeton glomeratus

Examples

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Embodiment 1

[0023] Embodiment 1: a method for directly observing the salinity content of different tissues of Halophysis saline, its main feature is that the steps are as follows:

[0024] (1) Sample collection: Take the potted halophyte seedlings cultivated under NaCl stress for 1-2 months as the object, first take out a single halophyte plant from the pot, rinse the plant with ultrapure water, and separate the roots, stems, and leaves in turn tissue, and cut with a double-sided blade;

[0025] (2) Agar embedding and cutting: use agar with a mass fraction of 3-5% to embed the roots, stems and leaves obtained in step (1) within 3 minutes and cut them off, leave them at room temperature for 3-5 minutes, and after the agar has solidified, use The double-sided blade cuts the embedded material into slices within 2 minutes;

[0026] (3) Liquid nitrogen freezing: put the material slices obtained in step (2) into liquid nitrogen within 1 min and freeze until the freezing is complete;

[0027] ...

Embodiment 2

[0033] Embodiment 2: A method for directly observing the salinity content of different tissues of Halophysis saline, the steps are as follows:

[0034] a. Sample collection: take potted grass seedlings cultivated for 1-2 months without NaCl stress (control) as objects, first take out a single plant of halophytes from the flower pot, wash 3 times with ultrapure water, separate roots, Stem and leaf tissue were cut into 1.0 cm cuts with a double-sided blade.

[0035] b. Agar embedding and cutting: use 3% agar to embed the roots, stems, and leaves obtained in step a within 3 minutes, cut off the agar, let it stand at room temperature for 5 minutes to solidify the agar, and quickly cut the embedded material with a double-sided blade Cut into slices with a thickness of 1-2mm.

[0036] c. Liquid nitrogen freezing: put the material slices obtained in step b into liquid nitrogen within 30 seconds and freeze for 3 minutes.

[0037] d. Freeze-drying: Place the completely frozen sample ...

Embodiment 3

[0039] Embodiment 3: a method for directly observing the salinity content of different tissues of Halophysis saline, the steps are as follows:

[0040] a. Sample collection: take 200mM NaCl stress cultured potted grass seedlings for 1-2 months as the object, first take out a single plant of halophytes from the flowerpot, wash it with ultrapure water for 3 times, and separate the roots, stems, and leaf tissues in turn , and cut into 0.5cm cuts with a double-sided blade.

[0041] b. Agar embedding and cutting: use 5% agar to embed the roots, stems, and leaves obtained in step a within 2 minutes, cut off the agar, let it stand at room temperature for 4 minutes to solidify the agar, and quickly cut the embedded material with a double-sided blade Cut into slices with a thickness of 1-2mm.

[0042] c. Liquid nitrogen freezing: put the material slices obtained in step b into liquid nitrogen within 1 min and freeze for 2 min.

[0043] d. Freeze drying. Place the completely frozen s...

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Abstract

The invention relates to a method for directly observing the salt content of different tissues of halophytes. Specifically, the use of scanning electron microscope combined with energy dispersive X-ray spectrometer to analyze the salinity in the sections of tissues and organs such as leaves, stems and leaves of succulent halophytes (mainly referring to Na + ) constitutes a method for qualitative and quantitative analysis. The process mainly includes the collection of saline grass roots, stems, and leaf tissue samples, agar embedding, section cutting, liquid nitrogen freeze-drying, section electron microscope scanning, and X-ray element composition for qualitative and quantitative analysis. The method of the invention has accurate, intuitive and reliable observation results on the salinity content of different tissues, and has important practical significance for the current research on the salt-tolerant mechanism of halophytes and the improvement of saline-alkali soil by utilizing the characteristics of salt-enrichment of halophytes.

Description

technical field [0001] The invention relates to a method for directly observing the salt content in different tissues of the halophyte halophyte. Background technique [0002] Halogetonglomeratus belongs to the annual halophytic herbaceous plant of Chenopodiaceae, and it is the dominant plant population in the saline-alkali land and desert area in the arid area of ​​Northwest my country. In the saline-alkali environment, the salt absorbed by the root tips of halophytes from the soil is mainly transported from the root to the stem and then to the leaves, and finally a large amount of salt is stored in the water storage tissue of the extremely fleshy leaves, which greatly slows down the growth rate in the saline-alkali soil. The ion poisoning caused by a large amount of salt ions in the medium (mainly Na + and Cl - ) and osmotic stress, realized the normal growth of halophytic grass in saline-alkali desert land. The salt-enrichment characteristic of the succulent leaves is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/22G01N23/00
CPCG01N23/2202G01N23/00G01N23/2206G01N2223/071G01N2223/1016
Inventor 王化俊汪军成姚立蓉李葆春孟亚雄马小乐赖勇司二静杨柯任盼荣赵向田
Owner GANSU AGRI UNIV
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