The invention relates to a novel cultivation method for strawberry seedlings. The novel cultivation method for the strawberry seedlings overcomes the shortcomings of seedling cultivation in an open field. According to the novel cultivation method, the seedlings are healthy, the plant diseases and insect pests are reduced, and the yield of the seedlings is high. The novel cultivation method comprises the steps: 1), guaranteeing ventilation and light transmission of a greenhouse, removing weeds outside the greenhouse and arranging drainage ditches around the greenhouse; 2), adopting polyethylene films as greenhouse films, covering each greenhouse chamber with four greenhouse films, adopting two-piece compression joint on the top, arranging head wind ports on the greenhouse chambers, and meeting the requirement for shielding from rain strictly under the condition that the head wind ports are closed; 3), compacting the ground of the greenhouse chamber and covering the ground with ground fabric or a black mulching film; 4), installing an axial flow fan in each greenhouse chamber; 5), planting stock plants of strawberries in seedling cultivation grooves or nutrition pots or flowerpots, wherein the inner diameter of the nutrition pots or the flowerpots is larger than 18cm, and adopting nutrition pots with the inner diameter being 10cm to contain young plants; and 6), adopting a seedling cultivation matrix for the strawberries as a seedling cultivation matrix, or forming the seedling cultivation matrix by mixing turf, vermiculite and perlite according to an appropriate proportion.