The invention discloses a method of determining thermophysical parameters of rock soil and heat resistance of a vertical ground heat exchanger. The method includes steps: initializing test data and parameters of a drill hole and a buried pipe acquired in a thermal response experiment; initially setting rock soil heat conducting coefficient lambda s, rock soil volume heat capacity Cs and weight coefficient f corresponding to weight average temperature, and setting respective parameter ranges; using a column heat source model and a quasi-three-dimensional heat resistance model to calculate drillhole wall temperature Tb, I, ground heat exchanger inlet-outlet fluid temperature Tin, sim, i, Tout, sim, I and ground heat exchanger heat resistance Rb, I corresponding to each moment in the experiment; using square sum mean value of measured value and simulation value difference of the weight average temperature as a target function fopt, and using a simulated annealing algorithm to perform optimization iteration on lambda s, Cs and f; when fopt is lower than an optimization target value delta Tset, acquiring optimization results of three parameters lambda s, Cs and f, calculating a mean value of the sum of Rb, I at each moment corresponding to the optimization results, and acquiring heat resistance Rb of the ground heat exchanger. By the method, accuracy of recognition results on the thermophysical parameters of the rock soil and the heat resistance of the ground heat exchanger is improved.