The application relates to the field of
biomedical engineering and particularly discloses hollow
virus-like nanoparticles for corneal crosslinking as well as a preparation method and application thereof. In view of problems such as the need to destroy
epithelium in traditional crosslinking,
ultraviolet toxicity and insufficient penetration of sonodynamic carriers, the following scheme is adopted: ① a CTAB / NaOH
micelle is used as a template, a TEOS
cyclohexane solution is self-assembled to form
virus-like silica particles, a hollow
ruthenium-based carrier is obtained through RuCl3 deposition and Na2CO3
etching, and a Ru-TiO2
heterojunction mesoporous shell is formed by
coating tetrabutyl
titanate; ② the nanoparticles are dispersed into a 0.5-5 mu g / mL suspension and instilled on an eye surface, and 1.0-1.5 MHz, 2-3 W / cm2 pulse
ultrasound (duty ratio 50-70%, 10-20 min) is applied. The
advantage lies in that the
virus-like spike structure realizes non-invasive penetration (
fluorescence intensity 106.5 AU, 2.8 times that of a traditional carrier), the hollow
heterojunction improves SO4 ‑ yield, the anti-enzymolysis time reaches 8.2+ / -0.3 h (210% of the
riboflavin / UVA group), and the postoperative eye surface
irritation is 0 (ISO 10993-10). The hollow virus-like nanoparticles are suitable for
radical treatment of
keratoconus with a corneal thickness greater than 300 mu m.