18 results about "Trichomoniasis" patented technology

The invention relates to a primer composition for detecting genital tract infection related pathogens and drug-resistant genes thereof and application of the primer composition, and detection targets of the primer composition cover 69 genital tract infection related pathogens including HPV virus, candida disease, trichomoniasis, sexually transmitted diseases and the like. And an antibiotic strong associated drug resistance site for neisseria gonorrhoeae and mycoplasma genitalium. The primers in the primer composition not only can be independently used for PCR amplification, but also can be used for multiple PCR amplification in the same detection system, mutual interference among the primers is little, no obvious non-specific amplification exists, one-tube multiple PCR amplification reaction is efficiently realized under the condition that the number of the primers is up to 150, and the detection efficiency is high. Comprehensive screening of pathogens related to complex genital tract infection and drug resistance detection of neisseria gonorrhoeae and mycoplasma genitalium can be achieved through one-time detection, and the method has the advantages of being good in specificity, high in sensitivity and high in accuracy.

The present invention is related to deuterated secnidazole, a prodrug thereof, a hydrate thereof, a solvate thereof, a polymorph thereof, or a pharmaceutically acceptable salt thereof and its use for the treatment of bacterial vaginosis or trichomoniasis in a subject in need thereof. The present invention is also related to pharmaceutical compositions and methods and uses of treating bacterial vaginosis, trichomoniasis, amoebiasis, giardiasis, or a combination thereof.

The invention discloses application of copper sulfate in inhibition of trichomonad activity, in-vitro tests prove that copper sulfate can induce oxidative stress of trichomonad, interfere with sulfur-containing amino acid metabolism, destroy the structure of trichomonad cells and reduce the active oxygen content and hydrogenase membrane potential of trichomonad bodies, and the minimum inhibition concentration of copper sulfate on trichomonad within 48 hours is 640 micrograms / mL. In-vivo tests prove that copper sulfate is added into drinking water according to the concentration of 500 mu g / mL, and is periodically fed once every 15 days and continuously 4 days each time, so that the number of trichomonad in the oral cavities of laying pigeons of silver-feather king pigeons and young pigeons of white-feather king pigeons can be remarkably reduced, and meanwhile, the quality of pigeon eggs is improved, and the immunity and intestinal microbial flora are improved; the application method provided by the invention is safe, efficient and low in cost, the addition concentration of copper sulfate is easy to control, no drug residue risk exists, and the composition can replace traditional antibiotics such as metronidazole to be used for preventing and treating trichomonad of pigeons, is suitable for a large-scale meat pigeon breeding scene and has a wide application prospect.

The application discloses a compound medicament for treating trichomonas gallinae, a preparation method of the compound medicament and a medicine, and aims to provide a novel, rapid and safe treatment formula which can replace metronidazole in treating trichomonas gallinae disease. The compound medicament prepared by mixing the aqueous extract of Sophora flavescens and the copper-containing adjuvant can kill the trichomonas gallinae in the pigeon in a short time, and cannot cause drug resistance and drug residue problems, and the formula of the application is simpler and has a shorter medication time than other traditional Chinese medicine formulas.

Devices, systems and methods are provided for treating conditions of the reproductive tract. A number of conditions can afflict the lining and cell layers deeper within the anatomical structures. For example, cervical intraepithelial neoplasia (CIN), also known as cervical dysplasia, is a condition involving abnormal growth of cells on the surface of the cervix that could potentially lead to cervical cancer in situ (CIS). Other conditions include human papillomavirus (HPV)-related cervical disease, various endometrial diseases, acute and chronic cervicitis, and various infections (e.g. trichomoniasis) to name a few. In some embodiments, treatments eliminate diseased, damaged, abnormal or otherwise undesired cells leaving the tissue framework intact. This allows the tissue to regenerate in a normal fashion, avoiding the formation of scar tissue. When the tissue framework is left intact, the framework structure repopulates with healthy cells, regenerating the normal tissue without altering the structural properties.

Devices, systems and methods are provided for treating conditions of the reproductive tract. A number of conditions can afflict the lining and cell layers deeper within the anatomical structures. For example, cervical intraepithelial neoplasia (CIN), also known as cervical dysplasia, is a condition involving abnormal growth of cells on the surface of the cervix that could potentially lead to cervical cancer in situ (CIS). Other conditions include human papillomavirus (HPV)-related cervical disease, various endometrial diseases, acute and chronic cervicitis, and various infections (e.g. trichomoniasis) to name a few. In some embodiments, treatments eliminate diseased, damaged, abnormal or otherwise undesired cells leaving the tissue framework intact. This allows the tissue to regenerate in a normal fashion, avoiding the formation of scar tissue. When the tissue framework is left intact, the framework structure repopulates with healthy cells, regenerating the normal tissue without altering the structural properties.

Method of treating or preventing trichomoniasis or T. vaginalis infection in a subject in need thereof, the method involving administering to the subject a therapeutically effective amount of secnidazole or a pharmaceutically acceptable salt in a microgranule formulation, wherein the microgranule formulation comprises a plurality of microgranules. The subject can also have bacterial vaginosis, is HIV-positive, and / or is suffering with metronidazole-resistant trichomoniasis and / or tinidazole-resistant trichomoniasis. The subject can also be a sexual partner of a person with trichomoniasis. The microgranule formulation can also be administered with paromomycin, tinidazole, metronidazole, boric acid or a combination thereof. Pharmaceutical compositions and uses for treating or preventing trichomoniasis or T. vaginalis infection in a subject in need thereof are also contemplated herein.

The application discloses a medicine composition for killing trichomonas vaginalis, and the active ingredients of the medicine composition are composed of fibraureus and metronidazole; the mass ratio of the fibraureus and the metronidazole is 1:1-8:1; in the process of killing the trichomonas vaginalis, the concentration of the fibraureus is 0.01-0.04 g / L, and the concentration of the metronidazole is 0.005-0.02 g / L. The medicine composition can effectively kill the trichomonas vaginalis, the fibraureus combined with the metronidazole can effectively reduce the medication time and the dosage of the metronidazole, can avoid the risk of premature delivery of pregnancy medication, and can reduce the health problems of breast-feeding infants during the medication period; the problems of adverse reactions and drug resistance of the metronidazole and other nitroimidazole drugs in treating trichomonas vaginalis disease are solved; the application provides a theoretical basis for treating drug-resistant trichomonas vaginalis vaginitis by combining traditional Chinese medicine with western medicine, and further expands the clinical application of the fibraureus.

This invention discloses an induction culture medium and a culture method for isolating *Hypericum sinense* strains from *Cordyceps sinensis* branches. The induction culture medium includes sucrose 5-20 g / L, starch 5-20 g / L, potassium nitrate 1-5 g / L, ammonium nitrate 4-8 g / L, zinc sulfate 5-20 mg / L, glycine 1-5 mg / L, calcium chloride 1-5 g / L, magnesium sulfate 1-2 g / L, ascorbic acid 0.3-0.6 g / L, NAA 0.1-2 mg / L, β-cyclodextrin 5-20 g / L, and agar 20 g / L. The culture method includes: (1) generating aerial mycelia from *Cordyceps sinensis* branches; (2) culturing the aerial mycelia in the induction culture medium to obtain *Hypericum sinense* strains. The cultivation method provided by this invention can isolate the Chinese trichomoniasis strain from broken branches of Cordyceps sinensis, which can reduce or avoid resource waste, lower cultivation costs, is convenient and fast, has a high mycelial germination rate, and a fast strain growth rate, which is conducive to the large-scale promotion of artificial cultivation technology of Cordyceps sinensis.

A controlled release, antimicrobial gel for the treatment of vaginal infections, comprises a muco-adhesive gel matrix containing one or more anti-infective agents bound to said matrix and effective for the treatment of vaginal infections and capable of pH dependent delivery of said one or more anti-infective agents as a single application to the vaginal cavity over a period of 3 days, while the muco-adhesive gel matrix remains bound to the vaginal epithelia. The preferred anti-infective agents are thymol and carvacrol in combination. The antimicrobial gel is biocidal against biofilms formed by the causative agents of bacterial vaginosis, trichomoniasis and candidiasis. The antimicrobial gel is effective for achieving sustained delivery of the anti-infective agents to the vaginal cavity at a therapeutically efficacious dose from a single application of the gel for the treatment and prevention, and prevention of recurrence, of bacterial vaginosis, trichomoniasis and candidiasis.

The invention discloses trametes hirsutum Zjut HJ2301 and application thereof in preparation of galactooligosaccharide through solid state fermentation, the strain can efficiently produce extracellular beta-galactosidase through solid state fermentation, and a solid substrate (such as agricultural waste) is directly used as a carbon source, so that the cost of a culture medium is reduced, a large amount of wastewater generated by liquid state fermentation is avoided, and the production cost is reduced. Pollution caused by infectious microbes in the fermentation process can be reduced in a low-moisture environment; compared with intracellular beta-galactosidase, extracellular enzyme is directly secreted into the environment, steps of cell disruption and the like are not needed, operation is simpler and more convenient, the enzyme recovery rate is higher, higher stability is achieved, the beta-galactosidase can directly act on a substrate, participation of microorganism living bodies is not needed, and safety is higher. The new strain is subjected to solid-state fermentation for 6 days by using wheat bran at 36 DEG C, the yield of beta-galactosidase can reach 23.79 U / g dry basis, and the yield of galactooligosaccharide generated by catalyzing lactose can reach 32%.

The invention discloses a Chinese honeylocust fruit extract for killing in-vitro cultured trichomonad pigeon and a preparation method of the Chinese honeylocust fruit extract, and belongs to the technical field of traditional Chinese medicines and the field of animal disease control, the Chinese honeylocust fruit extract is prepared by the following preparation method: crushing dry mature fruits of Chinese honeylocust fruits, performing condensation reflux extraction by using 70% ethanol, and collecting an extracting solution; concentrating by using a rotary evaporator; and drying at 55 DEG C by using an electrothermal blowing dry box to obtain the extract. According to the method, Chinese honeylocust fruit insecticidal components are extracted, and the method is easy to implement and convenient to operate. Compared with traditional chemical drugs, the alcohol extract prepared from medicinal saponin as a raw material has the advantages of no residue, no drug resistance, greenness and safety when being used for preventing and controlling trichomonad of pigeons, and has great popularization value. Meanwhile, the Chinese honeylocust fruit can effectively kill trichomonas pigeonae cultured in vitro, so that the adverse effect of trichomonas pigeonae on young pigeon growth is eliminated or reduced, and reference is provided for later application of the alcohol extract of the Chinese honeylocust fruit in trichomonas pigeonae resistance research and pigeon breeding.

To provide a composition for nucleic acid-based detection and identification of vaginal diseases, e.g., vulvovaginal candidiasis, trichomoniasis and / or bacterial vaginosis.SOLUTION: A buffer composition is provided comprising a bactericidal preservative comprising a conjugate pair of an acid and a base, a chelating agent or reducing agent, a non-ionic surfactant, a monovalent or divalent salt selected from the group consisting of sodium salts, potassium salts, calcium salts, magnesium salts, and combinations thereof, and optionally one or more isothiazolones.SELECTED DRAWING: Figure 5

The present invention relates to a primer composition for detecting pathogens related to reproductive tract infections and their drug-resistance genes, and its application. The detection targets of the primer composition include 69 pathogens related to reproductive tract infections, including HPV virus, candidiasis, trichomoniasis, sexually transmitted diseases, etc., as well as antibiotic-resistant sites strongly associated with Neisseria gonorrhoeae and Mycoplasma genitalium. The primers in the primer composition can be used individually for PCR amplification, and can also be used for multiplex PCR amplification in the same detection system. There is little mutual interference between primers, and there is no obvious non-specific amplification. In the case of up to 150 primers, a single-tube multiplex PCR amplification reaction can be efficiently achieved. A single test can achieve comprehensive screening of pathogens related to complex reproductive tract infections, as well as drug resistance detection of Neisseria gonorrhoeae and Mycoplasma genitalium, with the advantages of good specificity, high sensitivity, and high accuracy.

The invention discloses application of a sea-buckthorn extract to killing in-vitro cultured trichomonad pigeon and a preparation method of the sea-buckthorn extract, and belongs to the technical field of traditional Chinese medicines and the field of animal disease control, the sea-buckthorn extract is prepared by the following preparation method: crushing dried and ripe sea-buckthorn fruits, performing condensation reflux extraction by using 70% ethanol, and collecting an extracting solution; concentrating by using a rotary evaporator; drying in an electrothermal blowing drying box at 55 DEG C to obtain an extract; according to the method, the effective insecticidal components of the sea-buckthorn are extracted, and the method is easy to implement and convenient to operate; the alcohol extract is diluted according to a certain proportion and then used for killing trichomonad pigeon cultured in vitro, and the insecticidal effect is remarkable; compared with traditional chemical drugs, the alcohol extract prepared from the sea-buckthorn as the raw material is used for preventing and controlling trichomonad of pigeons, has the advantages of no residue, no drug resistance, greenness and safety, and has great popularization value.

The application provides use of waxberry tree bark in preparation of a medicine for preventing or treating parasitic infection or complications caused by parasitic infection, and belongs to the field of veterinary drugs. In the application, the waxberry tree bark can be used alone for killing of balantidium coli or prevention and treatment of chicken coccidiosis or avian trichomoniasis. The application has the characteristics of simple preparation and obvious prevention and treatment effect, and can be used for prevention and treatment of parasitic diseases in veterinary clinics, and has a higher insecticidal effect compared with many traditional Chinese medicinal materials.

This invention discloses the application of garlic essential oil in the preparation of an anti-Trichomonas vaginalis drug for pigeons. The garlic essential oil is prepared by steam distillation, and the drug is in the form of an emulsion for internal use. The invention first uses garlic essential oil in an in vitro inhibition test against Trichomonas vaginalis, determining the minimum inhibitory concentration (MIC) of the garlic essential oil. Trichomonas vaginalis was completely inhibited after 8 hours of treatment with a 50 μL / mL garlic essential oil emulsion. In vivo inhibition tests were also conducted using garlic essential oil. Each infected pigeon was administered 2.5 mL of a 20 μL / mL garlic essential oil emulsion (i.e., 50 μL of garlic essential oil). After 3 days of treatment, the oral trichomoniasis was completely cleared from the pigeons. During the treatment period, the pigeons did not experience any adverse reactions such as vomiting or diarrhea after oral administration. The garlic essential oil of this invention is naturally sourced, green and healthy, non-irritating to pigeons, and falls within the safe range for use, thus having significant application value in the preparation of an anti-Trichomonas vaginalis drug.