34 results about "Metronidazole" patented technology

The invention relates to application of an SLC30A9 inhibitor in preparation of an anti-tumor medicine, and belongs to the technical field of biological medicines. The invention provides an application of an SLC30A9 inhibitor in preparation of an anti-tumor drug. The expression of SLC30A9 is down-regulated to activate a cGAS-STING-NF-kappa B pathway, so that apoptosis of osteosarcoma cells is promoted, killing of the osteosarcoma cells by MTX is enhanced, and sensitivity of the osteosarcoma cells to MTX chemotherapy is recovered. Through combined application of SLC30A9 knock-down, a cGAS-STNG-NF-kappa B pathway agonist and MTX for chemotherapy, the killing effect of MTX on osteosarcoma cells can be remarkably enhanced, tumor cell proliferation is inhibited, and the treatment effect is improved. The core effect of the SLC30A9 in an osteosarcoma chemotherapy drug resistance mechanism is defined, and a theoretical basis and a technical support are provided for subsequent development of targeted drugs aiming at a cGAS-STNG-NF-kappa B pathway.

This invention relates to a method for preparing a nanozyme detection reagent for carbofuran residues in apples, belonging to the field of pesticide residue and food safety technology. Specific steps: (1) Prepare mesoporous polydopamine powder using polyoxyethylene-polyoxypropylene-polyoxyethylene triblock copolymer, dopamine hydrochloride, etc.; (2) Prepare a copper-manganese bimetallic carbon dot solution using citric acid, copper chloride, manganese chloride, and ethylenediamine; (3) Prepare the nanozyme detection reagent using the mesoporous polydopamine solution and the copper-manganese bimetallic carbon dot solution. Mesoporous polydopamine has a large specific surface area, providing numerous sites for carbon dot loading and effectively adsorbing carbofuran molecules. The copper-manganese bimetallic carbon dots give the nanozyme detection reagent good enzyme-like activity and generate pesticide concentration-dependent colorimetric and photothermal signals. This invention achieves mutual verification of dual-mode output signals, significantly improving detection reliability; and completes detection within 1.5 h.

The invention discloses a medicine for inhibiting helicobacter pylori infection, the medicine is oridonin, and the structural formula of the medicine is as shown in formula 1. In-vitro experiments show that the oridonin has a remarkable antibacterial effect on helicobacter pylori strains SS1, ATCC700392 and ICDC11101, and can remarkably reduce the urease activity of the helicobacter pylori, inhibit the formation of a biological membrane, destroy the growth of thalli and reduce the adhesion effect of the helicobacter pylori on gastric epithelial cells, and in addition, the oridonin and antibiotics are combined for use, so that the effect of preventing and treating the gastric epithelial cells can be achieved. The compound has a synergistic antibacterial effect with metronidazole and clarithromycin, has the effect of reducing the dosage of antibiotics in clinical application, and has potential application value for improving the current drug resistance situation of the antibiotics.

The invention discloses a bionic bone scaffold composite material prepared through combination of electrostatic spinning and 3D printing and a preparation method of the bionic bone scaffold composite material, and relates to the technical field of bionic bone scaffold composite materials. A bionic bone scaffold composite material prepared by combination of electrostatic spinning and 3D printing is characterized by comprising 80 to 100 parts of a biodegradable high-molecular polymer, 5 to 30 parts of a bioactive inorganic ceramic material, 200 to 500 parts of a solvent, 0.01 to 5 parts of a bioactive factor, 0.1 to 0.5 part of a bacteriostatic active compound and 0.01 to 5 parts of naringin. According to the invention, the metronidazole derivative is used for providing antibacterial ability, and is matched with naringin to realize anti-inflammatory and osteogenesis-promoting synergistic effect, so that the problem of drug resistance or non-uniform drug release of the traditional antibacterial means is avoided, the induction effect of the stent on bone regeneration is enhanced, and the infection risk in the repair process is reduced.

The invention discloses application of copper sulfate in inhibition of trichomonad activity, in-vitro tests prove that copper sulfate can induce oxidative stress of trichomonad, interfere with sulfur-containing amino acid metabolism, destroy the structure of trichomonad cells and reduce the active oxygen content and hydrogenase membrane potential of trichomonad bodies, and the minimum inhibition concentration of copper sulfate on trichomonad within 48 hours is 640 micrograms / mL. In-vivo tests prove that copper sulfate is added into drinking water according to the concentration of 500 mu g / mL, and is periodically fed once every 15 days and continuously 4 days each time, so that the number of trichomonad in the oral cavities of laying pigeons of silver-feather king pigeons and young pigeons of white-feather king pigeons can be remarkably reduced, and meanwhile, the quality of pigeon eggs is improved, and the immunity and intestinal microbial flora are improved; the application method provided by the invention is safe, efficient and low in cost, the addition concentration of copper sulfate is easy to control, no drug residue risk exists, and the composition can replace traditional antibiotics such as metronidazole to be used for preventing and treating trichomonad of pigeons, is suitable for a large-scale meat pigeon breeding scene and has a wide application prospect.

The invention relates to the technical field of wastewater treatment microorganisms, in particular to a detoxification synergistic treatment process for metronidazole-containing pharmaceutical wastewater, which comprises the following treatment steps: (1) metronidazole-containing pharmaceutical wastewater enters a dealdehyding tower in advance for dealdehyding treatment, and then enters a primary settling tank for settling to remove scum; (2) enabling the wastewater obtained in the step (1) to sequentially enter a hydrolysis acidification tank, a UASB anaerobic tank and a mid-sedimentation tank; (3) the wastewater is settled in the middle settling tank, enters an anoxic tank and an aerobic tank for biochemical treatment, is subjected to mud-water separation through a secondary settling tank, and is discharged; and (4) enabling the effluent of the secondary sedimentation tank to enter a deep treatment unit through a pipeline for treatment, then entering a decoloration tank for decoloration, and discharging. In order to improve the COD degradation efficiency of metronidazole-containing pharmaceutical wastewater in an anaerobic tank and stabilize the COD of the effluent, the invention provides a microbial agent which has strong adaptability to water quality and water quantity fluctuation and is stable in operation.

Disclosed is a bile acid-metronidazole conjugate or a stereoisomer thereof, or a salt thereof, or a solvate thereof, or a prodrug thereof, or a metabolite thereof. The bile acid-metronidazole conjugate is prepared using an esterification reaction of a bile acid compound with hydroxyl group on a metronidazole structure. The synthesis process is simple and easy for industrialization. The bile acid-metronidazole conjugate can effectively inhibit the growth of anaerobic bacteria, especially the growth of Clostridioides difficile and Helicobacter pylori, so that the bile acid-metronidazole conjugate has a significantly improved therapeutic effect in the treatment of infections caused by the anaerobic bacteria.

The invention discloses a metronidazole phospholipid composition as well as a preparation method and application thereof, and relates to the technical field of biological medicines, and the metronidazole phospholipid composition comprises metronidazole and phospholipid. The metronidazole phospholipid composition prepared by the invention improves the intracellular drug delivery efficiency under the condition of retaining the original chemical structure and biological activity of metronidazole, and a new method is provided for treatment of intracellular infection of anaerobic bacteria. The problem that existing metronidazole is poor in intracellular infection treatment effect is solved.

The invention relates to the technical field of fluorescence sensing, in particular to a dysprosium (III) coordination polymer as well as a preparation method and application thereof. The dysprosium (III) coordination polymer is prepared through a one-pot solvothermal reaction, the dysprosium (III) coordination polymer has an expression of [Dy2 (bppc) 5 (gluu) 2 (H2O) 4] n, and the preparation method has the advantages of being simple in process, convenient to operate, high in yield, good in reproducibility and the like. The dysprosium (III) coordination polymer prepared by the invention has better fluorescence property, shows high selectivity and high sensitivity to antibiotics, and can be used for effectively detecting metronidazole through a fluorescence quenching phenomenon. Meanwhile, according to the high-security information encryption system based on the dynamic optical characteristics of the Dy-based luminescent material, information counterfeiting and copying are effectively eradicated through luminescent signals capable of being randomly combined and a real-time refreshed'one-time dynamic password 'mechanism of the high-security information encryption system.

The application discloses a compound medicament for treating trichomonas gallinae, a preparation method of the compound medicament and a medicine, and aims to provide a novel, rapid and safe treatment formula which can replace metronidazole in treating trichomonas gallinae disease. The compound medicament prepared by mixing the aqueous extract of Sophora flavescens and the copper-containing adjuvant can kill the trichomonas gallinae in the pigeon in a short time, and cannot cause drug resistance and drug residue problems, and the formula of the application is simpler and has a shorter medication time than other traditional Chinese medicine formulas.

The present invention relates to the field of reducing methane emissions from ruminants. In particular, the present invention relates to administering to a ruminant a combination of 3-nitrooxypropanol and / or a derivative thereof and metronidazole for reducing methane production resulting from digestive activities of said ruminant.

This invention discloses the application of *Gastrococcus xanthophylloides* in regulating intestinal bile acid metabolism disorders, including *Gastrococcus xanthophylloides* (… Ruminococcus flavefaciens The application of this invention in the preparation of compositions for regulating intestinal bile acid metabolism disorders, wherein the compositions promote the conversion of primary bile acids to secondary bile acids, wherein the primary bile acids include cholic acid and chenodeoxycholic acid, and the secondary bile acids include deoxycholic acid and lithocholic acid; wherein the intestinal bile acid metabolism disorders include metabolic disorders caused by antibiotics, wherein the antibiotics include one or more of moxifloxacin and metronidazole; wherein the compositions can be health products, pharmaceuticals, or feed; and wherein the active ingredient of the compositions includes xanthospirococcus flavus.

The application discloses a preparation method of a rapid detection kit for metronidazole residue in rice, and belongs to the technical field of bio-agriculture. The method comprises the following steps: preparing a magnetic molecular imprinting enrichment agent, molybdenum-copper double-atom nitrogen-sulfur co-doped carbon dots, a hollow mesoporous polydopamine carrier and a colorimetric-photothermal dual-signal nanoscale enzyme reagent, and packaging the above reagents into a box together with acidolysis extraction liquid, substrate color developing liquid and standard comparison liquid. The kit can realize selective enrichment, matrix purification and colorimetric-photothermal dual-channel detection of metronidazole in rice samples, has the advantages of simple operation, rapid detection, strong anti-interference ability and high reliability of results, and is suitable for on-site rapid screening of metronidazole residues in paddy, brown rice and milled rice.

The application discloses a medicine composition for killing trichomonas vaginalis, and the active ingredients of the medicine composition are composed of fibraureus and metronidazole; the mass ratio of the fibraureus and the metronidazole is 1:1-8:1; in the process of killing the trichomonas vaginalis, the concentration of the fibraureus is 0.01-0.04 g / L, and the concentration of the metronidazole is 0.005-0.02 g / L. The medicine composition can effectively kill the trichomonas vaginalis, the fibraureus combined with the metronidazole can effectively reduce the medication time and the dosage of the metronidazole, can avoid the risk of premature delivery of pregnancy medication, and can reduce the health problems of breast-feeding infants during the medication period; the problems of adverse reactions and drug resistance of the metronidazole and other nitroimidazole drugs in treating trichomonas vaginalis disease are solved; the application provides a theoretical basis for treating drug-resistant trichomonas vaginalis vaginitis by combining traditional Chinese medicine with western medicine, and further expands the clinical application of the fibraureus.

The invention discloses a construction method and application of an Eriocheir sinensis gut-brain axis research model, according to the method, metronidazole, neomycin and vancomycin are added to commercial feed according to the mass ratio of 10: 10: 5 g / kg to prepare compound antibiotic feed, and the Eriocheir sinensis is continuously fed for 96 h. According to the method, a standardized research model capable of synchronously and controllably presenting the causal relation of'intestinal flora disturbance-nervous system response 'is constructed in crustacean for the first time. The model is short in construction period, high in stability and high in repeatability, and a key research tool is provided for deeply analyzing the enteric-cerebral axis interaction mechanism of the eriocheir sinensis and even crustacean, screening neural active substances and evaluating neurotoxicity of environmental stress.

The invention provides a preparation method of a seaweed active component for inhibiting helicobacter pylori, which comprises the following steps: cleaning seaweed, and drying in a drying oven at 60 DEG C until the weight is constant; seaweed and distilled water are prepared into a seaweed suspension according to the ratio of (0.5-1.5) g: (50-100) mL; adding 2-3% g / g glucose into the seaweed suspension, and performing pasteurization at 65 DEG C for 30 minutes; adding lactobacillus into the seaweed turbid liquid according to the volume ratio of 4-6% v / v, and fermenting at the constant temperature of 37 DEG C for 46-50 hours to obtain a seaweed fermentation mixed liquid; centrifuging the seaweed fermentation mixed solution to remove seaweed residues and thalli to obtain a seaweed fermentation product; and carrying out solvent distribution extraction on the seaweed fermentation product, taking an organic phase, carrying out rotary evaporation to remove the organic solvent, and washing with methanol to obtain the seaweed active component. The diameter of a helicobacter pylori inhibition zone of the seaweed active component reaches 1.8 cm, and compared with a positive control (5 mg / mL metronidazole), the bacteriostasis rate can reach 77.25%.

The present invention relates to a composition for preventing or treating periodontal disease and a dental composite antibiotic ointment composition. The composition for preventing or treating periodontal disease according to the present invention comprises minocycline and metronidazole as effective ingredients.

The invention discloses a construction method and application of an Eriocheir sinensis intestinal flora deletion model, the method comprises the following steps: feeding Eriocheir sinensis with a feed mixed with mixed antibiotics for 96 hours to obtain the Eriocheir sinensis intestinal flora deletion model; the mixed antibiotic is prepared from metronidazole, neomycin and vancomycin. A plate culture method verifies that the optimized core antibiotic concentration group can enable the clearance rate of intestinal content florae to reach 95% or above and the clearance rate of fecal florae to reach 90% or above. According to the method, the intestinal flora deletion model of the eriocheir sinensis is constructed for the first time, the method has the advantages of being high in removal efficiency, good in repeatability, small in stress and the like, the flora is restorable after drug administration is stopped, and a standardized experimental tool is provided for studying the intestinal-brain axis mechanism, physiological metabolism and healthy breeding of crustacean.

The application discloses a metronidazole co-crystal and a preparation method and application thereof, and a structure formula of the co-crystal is shown as formula I: the metronidazole co-crystal has a faster dissolution rate and a higher apparent solubility, improves a peak concentration thereof, and can reach a higher blood drug concentration in a short time, so that rapid control on infection symptoms is realized.

This invention provides an insecticide containing a pyrimidinone metronidazole compound and its application, belonging to the field of pesticide technology. The active ingredient of this insecticide comprises a pyrimidinone metronidazole compound as shown in Formula I; (I); where R... 1 It is 2-chlorothiazol-5-yl or 6-chloropyridin-3-yl, R 2 and R 3 It can be H, F, Cl, Me, or CN. This insecticide is used to control invertebrate pests. This invention discloses a pyrimidinone metronidazole compound, which, compared with conventional insecticides such as trifluralin and flonicamid, exhibits significantly improved activity and enhanced toxicity for pest control.

The invention provides a related medicine for treating senile diseases and application, the related medicine is used for inhibiting or removing methylmalonic acid in a body, and the related medicine is selected from at least one of coenzyme A, hydroxycobalamin, levocarnitine, betaine, carglutamic acid, metronidazole, neomycin, ACSF3 protein or an activator. According to the invention, the level of methylmalonic acid in vivo can be significantly reduced by using a removal technology (hemodialysis or application of a methylmalonic acid scavenger); after the methylmalonic acid is removed, the condition of senescence diseases can be obviously improved; mSC rejuvenation can be significantly increased by inhibiting methylmalonic acid.

This invention provides a probiotic composition for the prevention and / or treatment of vaginal infections and related diseases, and its use therein. The probiotic composition comprises *Lactobacillus plantarum* and *Lactobacillus fermentum*, wherein the gene sequence of the molecular marker of *Lactobacillus plantarum* has the nucleotide sequence shown in SEQ ID NO: 1, or the nucleotide sequence thereof is shown in SEQ ID NO: 1; and the gene sequence of the molecular marker of *Lactobacillus fermentum* has the nucleotide sequence shown in SEQ ID NO: 2, or the nucleotide sequence thereof is shown in SEQ ID NO: 2. The probiotic composition of this invention achieves better efficacy in treating vaginal infections, especially vulvovaginal candidiasis, compared to single strains therein and existing positive control drugs such as metronidazole suppositories.

The application discloses a high-efficiency metronidazole soil fumigant, which is prepared from the following raw materials in percentage by weight: metronidazole 35-45%, wetting agent 0.4-1.5%, penetrating agent 0.4-2.5%, defoaming agent 0.1-0.3%, and water. The metronidazole soil fumigant has high wetting and penetrating properties, improves soil disinfecting effect, and simplifies the disinfecting process. The application further discloses a method for improving the penetrating property of the metronidazole soil fumigant. The method is simple and easy to operate, and does not need special instruments and equipment.

Topical vaginal compositions are disclosed herein which include silica microspheres and an active ingredient in the amount of 0.001 to 15.0% w / w selected from estradiol, metronidazole, clindamycin, butoconazole, and combinations thereof. The topical compositions provide sustained release of the active ingredient so as to reduce skin irritation.

FIELD: chemistry; pharmaceuticals.SUBSTANCE: method for producing microcapsules with metronidazole by ionic gelation in combination with electrospray. The proposed method includes: preparing a capsule-forming solution by compounding a metronidazole suspension in vaseline oil, then dissolving sodium alginate and sparsely cross-linked polymethacrylic acid in water, adding Tween-80 to the resulting solution, and then introducing the said metronidazole suspension with constant stirring using a dispersant; preparing a gelling solution by using an aqueous solution of a basic polymethacrylic acid derivative with additionally added metronidazole and calcium chloride; spraying the capsule-forming solution into the gelling solution using an encapsulator; after which the resulting microcapsules are filtered, washed, and dried.EFFECT: reliable encapsulation of water-soluble metronidazole from an oil suspension in an aqueous-salt medium without the use of organic solvents.1 cl, 6 dwg

The application provides a high-sensitivity detection method for multi-residues of veterinary drugs in pig urine, and belongs to the technical field of detection of veterinary drug residues. The application first performs enzymatic treatment on the sample to be detected, thereby improving the detection sensitivity of salbutamol and ractopamine; then performs acid hydrolysis and derivatization treatment on the sample to be detected, thereby improving the reversed-phase chromatographic retention performance and mass spectrometry ionization efficiency of nitrofurans in the compound to be detected, improving the sensitivity, improving the polarity of the compound to be detected, thereby improving the chromatographic separation effect, effectively separating the interfering substances in the matrix, improving the anti-interference ability of the detected substance, and indirectly improving the detection sensitivity; clenbuterol, metronidazole, dimetridazole, olaquindox, chlorpromazine and chloramphenicol are mainly in free state, and are not affected by enzymatic and acid hydrolysis and derivatization during detection, can be detected together with the above-mentioned compounds to be detected, and the mass spectrometry derivatization and sensitivity detection of multi-residues of cross-class veterinary drugs are realized.

This invention discloses the application of the compound pimozide in combination with metronidazole in the preparation of a drug for improving oocyte quality and treating endometriosis-related fertility disorders, belonging to the field of biomedical technology. The efficacy of this invention was verified by constructing mouse models of endometriosis driven by pathogenic *Prevotella bisporus* and mouse models of ovarian endometriosis. Experimental results showed that the combined administration significantly eliminated *Prevotella bisporus* colonizing ectopic lesions, reduced succinic acid content in both eutopic and ectopic lesions, decreased the succinylation level of lysine residue 195 of the ACADVL protein, reduced the fluidity of primary endometrial epithelial cell membranes, reduced the volume and weight of endometriotic lesions, and improved oocyte spermatogenesis, ovulation rate, and litter size. This invention solves the problem that existing therapies cannot simultaneously control endometriosis lesions and protect reproductive function, providing a new, safe, and effective combined treatment option for patients with endometriosis and infertility.

This invention provides a probiotic composition for the prevention and / or treatment of vaginal infections and related diseases, and its use therein. The probiotic composition comprises *Lactobacillus curvatureii*, *Lactobacillus jenny*, *Lactobacillus gasseri*, *Lactobacillus plantarum*, and *Lactobacillus fermentum*. Experiments have demonstrated that the probiotic composition of this invention achieves better efficacy in treating vaginal infections, especially VVC, compared to single-strain probiotics and positive control drugs such as metronidazole suppositories and diclofenac sodium.

According to the deciduous tooth root canal filling paste and the preparation method, iodoform, metronidazole, ZnO and Ca (OH) 2 are adopted as main components, the components have a wide application basis in the field of oral medicine and are high in safety and good in biocompatibility, and it can be ensured that the performance of the paste is stable and reliable by accurately controlling the proportion of all the components; iodoform and metronidazole are used as main components of the paste, have a remarkable antibacterial effect and can effectively kill bacteria in root canals, reduce infection risks and create a sterile environment for healing of periapical tissues, and due to the antibacterial effect, the success rate of root canal treatment is increased, the treatment cycle is shortened, and the treatment cost is reduced. The number of times of re-consultation and pain of the patient are reduced; znO and Ca (OH) 2 have excellent biocompatibility and cannot stimulate or damage periapical tissues, so that the safety of a treatment process and the comfort of a patient are guaranteed, and meanwhile, the components can also promote regeneration and repair of the periapical tissues.

The invention discloses a benzoyl metronidazole crystal as well as a preparation method and application thereof. The benzoyl metronidazole crystal comprises spherulitic benzoyl metronidazole, and the Span value of the spherulitic benzoyl metronidazole is less than 2.00. The spherocrystal benzoylmetronidazole is smooth in surface, regular in morphology, good in fluidity and high in bulk density, the caking problem of the benzoylmetronidazole product can be reduced or prevented, and the product is convenient to store and transport. The preparation method of the spherocrystal benzoylmetronidazole is simple to operate and controllable in condition, and the obtained product is good in morphology reproducibility and reliable in result.