14 results about "Inflammatory lesion" patented technology

The present invention relates to the technical field of biology, and particularly relates to a method for inhibiting an inflammatory response by using a fibroblast. In the present invention, a fibroblast, which is obtained by digesting normal foreskin tissue by means of clostridiopeptidase A to isolate a cell as a seed cell and culturing the seed cell in vitro by using a serum-free medium, shows positive expression of CD105, CD90, CD73, COL1A1, and FAP, with expression rates all exceeding 80%, and negative expression of CD36, CD39, CD45, and HLA class II molecules, with expression rates below 5%. The fibroblast can regulate an immune response by secreting TGFβ, inhibiting proliferation of PBMCs, thereby inhibiting the inflammatory response, and has been verified in an animal experiment to have the ability to repair knee osteoarthritis caused by meniscus injury. Therefore, the fibroblast can be used for preventing and treating inflammatory lesions such as osteoarthritis.

The application discloses a lung inflammation lesion quantitative evaluation artificial intelligence model based on CT images and relates to the field of artificial intelligence models.The model comprises CT image preprocessing, self-supervised lesion segmentation and quantification, multi-modal joint pre-training coding and multi-task diagnosis and prognosis prediction module composition which are sequentially and communicatively connected, and realizes feature interaction through a unified feature embedding space.The model realizes self-supervised lesion segmentation and multi-dimensional quantification by adopting a three-dimensional generative reconstruction network, completes image-text feature fine-grained alignment through cross-modal contrast learning of an adversarial enhancement, and fuses multi-dimensional features.The model can complete multiple tasks such as ARDS diagnosis, non-invasive estimation of P / F ratio, severity grading and prognosis prediction in parallel, reduces dependence on artificial labeling, improves cross-center generalization capability and diagnosis precision, and provides a standardized intelligent tool for clinical evaluation of severe lung inflammation.

The invention discloses preparation and application of macrophage targeting nanoparticles entrapped with a cell burial function accelerant. The medicine for enhancing the intercellular function of the macrophages is a liver X receptor stimulant, and the liver X receptor stimulant comprises at least one of T0901317, GW3965, Ouabagenin and LXR-623. The surface of the targeting nanoparticle is coated with a macrophage cell membrane, and meanwhile, a urokinase type plasminogen activator receptor (uPAR) targeting ligand is modified. The nanoparticle can be efficiently accumulated at an inflammatory focus part by utilizing a macrophage cell membrane, and the modified targeting ligand is used for targeting the macrophage with damaged cell burial function, so that the cell burial function of the macrophage is enhanced, the polarization of the macrophage to an anti-inflammatory phenotype is promoted, the release of anti-inflammatory and proinflammatory cytokines is regulated, and the anti-inflammatory effect of the nanoparticle is improved. And finally, the inflammatory microenvironment of a focus part is regulated and controlled, so that a good effect of treating autoimmune diseases is achieved.

A small activating RNA (saRNA) capable of activating a CCAAT enhancer binding protein alpha (CEBPA) gene, and a delivery system and use thereof are provided, where sense and antisense strands of the saRNA have nucleotide sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 2, respectively. A biomimetic nano-delivery system for targeted delivery of the saRNA is a biomimetic nanoparticle formed by composite nanoparticle coated by a biomembrane of an inflammatory effector cell, where the composite nanoparticle is formed by histone loading with the saRNA capable of activating the CEBPA gene. The biomimetic nanoparticle can inherit antigens and related membrane functions of the inflammatory effector cell and specifically accumulate at the sites of inflammatory lesions, which enables the biomimetic nanoparticle to not only have a longer circulation time in vivo, but also show inflammatory tropism.

Disclosed are a preparation method and use of a diagnosis-treatment integrated antibody-drug conjugate for autoimmune diseases. The preparation method comprises the following steps: mixing a contrast agent with an antibody and performing coupling via a physical reaction or a chemical reaction to give a contrast agent-antibody conjugate; and then mixing a drug with the contrast agent-antibody conjugate and performing coupling via a physical reaction or a chemical reaction to finally give the antibody-drug conjugate. The prepared antibody-drug conjugate has near-infrared fluorescence imaging and CT imaging characteristics, can target an inflammatory lesion, and can be used for diagnosing or assisting the diagnosis of autoimmune diseases. Meanwhile, the antibody-drug conjugate has a good anti-inflammatory effect and can ameliorate symptoms such as pain and improve urination functions after treatment. It achieves treatment effects by adjusting the diversity and abundance of the topical microbiota, thereby treating autoimmune diseases.

The invention discloses a construction method and application of a self-assembled air bag of a targeted inflammatory factor. The construction method comprises the following steps: carrying out engineering modification on an air bag gene sequence, so that protein on the outer surface of the air bag is directionally fused, and expressing an oligopeptide affinity tag; designing a bispecific nano antibody gene sequence; co-transforming a balloon gene and a bispecific nano antibody gene into a host cell to realize self-assembly; and crushing the host cells which successfully express the self-assembled air bag, and carrying out centrifugal separation to obtain the self-assembled air bag of the targeted inflammatory factor. According to the invention, the self-assembled airbag of a biological source is prepared in a pure biosynthesis mode, high targeting to inflammatory factors can be realized without chemical modification of the airbag again, the airbag has good biocompatibility and can specifically target to inflammatory lesions, and through ultrasonic echo signals generated by gas in the airbag, the self-assembled airbag can be used for detecting the inflammatory lesions. The medical image contrast of an inflammation part is obviously enhanced, and the key problem that early inflammation focus imaging is difficult is effectively solved.

The application provides mesenchymal stem cells overexpressing IL-1Ra and application thereof, and belongs to the technical field of biological medicine. By overexpressing IL-1Ra in MSCs, the migration function of the MSCs is significantly improved, and the number of MSCs homing to the tissue damage site is significantly increased; in addition, the MSCs overexpressing IL-1Ra can also secrete IL-1Ra, so as to achieve the synergistic anti-inflammatory effect with the MSCs, thereby significantly improving the anti-inflammatory ability and tissue damage repair ability of the MSCs, and improving the treatment effect of the MSCs. The MSCs overexpressing IL-1Ra can be used for treating various local or systemic inflammatory diseases, and has good curative effect in the treatment of hemorrhagic cystitis and acute pulmonary inflammatory lesion diseases.

The invention relates to application of a qi-regulating and blood-activating preparation in preparation of a medicine for preventing or treating heart failure, and belongs to the technical field of medicine application. The qi-regulating and blood-activating preparation is prepared from fructus cinnamoii, blumea balsamifera, ligusticum wallichii and allium macrostemon, and the preparation can be a dripping pill, a capsule, a tablet, a granule or a solution. Experiments show that the qi-regulating and blood-activating preparation can effectively treat heart failure, especially heart failure after myocardial infarction, and can reduce the infarction area of the heart, improve inflammatory lesion of the heart and improve myocardial fibrosis; the proliferation of myocardial cells induced by blood oxygen deprivation can be promoted, and the apoptosis of the myocardial cells can be improved. The use is convenient;

The invention relates to the technical field of noninvasive medical imaging, acousto-optic coupling and targeted drug delivery, and discloses a lesion finding and positioning and targeted drug guiding system and method based on an acousto-optic coupling functional film layer. Based on intelligent processing equipment with acoustics, optics and signal processing capacity, non-invasive discovery and accurate fault positioning of human tissue / body fluid lesions and directional guidance and local enrichment of targeted drugs are achieved in a time-sharing mode through the same physical area of the same acousto-optic coupling integrated functional film layer. The membrane layer multiplies the frequency of a low-frequency signal of 20Hz-20kHz into medical ultrasound of 2MHz-10MHz to complete imaging positioning, and meanwhile, targeted drug guidance such as optical / acoustic power, magnetic targeting and ultrasonic transdermal infiltration promotion is realized through the special optical / energy conducting layer, so that the drug focus enrichment rate is increased, and the side effects of the whole body are reduced. Equipment hardware does not need to be transformed, the film layer is flexible in form and wide in adaptability, medical diagnosis and treatment functions are integrated into consumer electronic equipment for the first time, low-cost popularization is achieved, and the system is suitable for targeted drug treatment guidance of various lesions such as solid tumors, virus infection lesions and chronic inflammation lesions, can be applied to multiple scenes such as families, primary medical treatment and hospitals and has wide application prospects. And the method has the remarkable advantages of high precision, noninvasive safety and the like.

The present invention relates to mixtures, or compositions comprising said mixtures, comprising at least one bacterial strain, preferably at least one probiotic bacterial strain or a derivative thereof, as well as to the use of said mixtures or compositions in methods for the preventive and / or curative treatment of an inflammatory ocular and / or periocular lesion, preferably in methods for the treatment of ocular diseases such as dry eyes, eyelid allergy, conjunctivitis, blepharitis, meibonite, dacryoliths, stye, inflammatory lesions of ocular adnexa and associated symptoms.

The application provides a method for constructing a zebrafish secondary hemophagocytosis syndrome model and application, relates to the technical field of animal model construction, and comprises the following steps: administering CpG ODN 2007 to zebrafish every other day; meanwhile, administering lipopolysaccharide to the zebrafish every day; and the administration time is at least 7 days. By using lipopolysaccharide and CpG ODN 2007 to jointly administer the zebrafish, the zebrafish secondary hemophagocytosis syndrome model is constructed, the construction method is short in time and small in drug dosage, the modeling time is shortened, and the obtained zebrafish model can realize multidimensional evaluation covering the number of whole blood cells, the expression amount of inflammatory factors and the inflammatory lesion area of the liver, the spleen and the kidney, thereby providing important significance for the research on HLH rare diseases.

This invention relates to the field of chemical pharmaceutical formulation manufacturing technology, and discloses a shikonin composition for inhibiting non-specific inflammation of the reproductive system, comprising: nanomicelles formed by encapsulating shikonin with tocopherol polyethylene glycol 1000 succinate, phenylboronic acid-modified sodium hyaluronate, and a lactic acid buffer system. The phenylboronic acid-modified sodium hyaluronate constructs a gel anchoring layer by complexing the phenylboronic acid groups with the ortho-dihydroxy structure on the action surface. This invention utilizes hydrogen peroxide to induce an oxidative deboronization reaction at the inflammatory lesion, causing an in-situ phase transition from a gel state to a sol state in the gel anchoring layer, eliminating drug diffusion resistance, and resolving the mismatch between mucosal clearance and drug release rate. The composition enhances the chemical stability of shikonin while achieving feedback regulation of drug dosage and inflammation degree, effectively repairing the mucosal barrier and maintaining local microecological balance.

The present application relates to transrectal photoacoustic ultrasonic diagnosis and treatment probe, diagnosis and treatment system and photoacoustic ultrasonic dual-mode imaging guiding method, the photoacoustic ultrasonic diagnosis and treatment probe includes probe part, ultrasonic part, two photoacoustic parts and imaging part.The advantage is that the ultrasonic part and the imaging part are separated in physical space, the transmission and reception are separated, the imaging echo signals excited by the ultrasonic part and the photoacoustic part are received by the imaging part respectively, photoacoustic-ultrasound fusion imaging is carried out, the detection of inflammatory lesions is improved, the position of the lesion is given, and accurate guidance is provided for subsequent integrated treatment;By using the miniaturized photoacoustic ultrasonic diagnosis and treatment probe, it is convenient to put into the rectum through the anus, and only one probe is used to realize the diagnosis and treatment integrated function;Through the phase control time division multiplexing technology, the ultrasonic part is quickly and accurately switched between different functions, and the imaging and treatment requirements are met;The ultrasonic treatment intensity is accurately controlled, and the treatment is realized under the premise of protecting the defense function of the blood-prostate barrier.

The invention discloses a nano-particle coated and loaded with ginsenoside by a pre-activated microglial cell membrane, a preparation method of the nano-particle and application of the nano-particle in depression. Comprising the following steps: coating the surfaces of nano-particles of ginsenoside Rb1 with a pre-activated microglial cell membrane to prepare the nano-particles of which the pre-activated microglial cell membrane is coated and loaded with ginsenoside. According to the application, the GRb1-coated PLGA and the GRb1-coated PLGA-coated AM can be recruited to the brain of a depression mouse, so that accumulation of the GRb1-coated PLGA-coated AM in an inflammatory lesion part is increased, the efficient targeting function of the depression inflammation part is realized, the progress of depression can be effectively inhibited, and good biological safety is achieved.