30 results about "Macrophage targeting" patented technology

The invention belongs to the field of pharmacy, and discloses a synthesis method of a macrophage targeting carrier system and a preparation method of a gene delivery system. The macrophage targeting carrier system is characterized in that a macrophage targeting carrier is mannosylation protamine; electropositive mannosylation protamine loads electronegative nucleic acid to form a positively charged nano particle; and protamine modified by carubinose is prepared from formyl methyl mannopyranoside and protamine sulfate through a reductive amination reaction. Compared with non-viral gene carrier protamine, mannosylation protamine has a nuclear localization function and a macrophage targeting property, and can improve the gene transfection mediation efficiency of protamine in macrophage. The preparation method is simple, and mature in technology, and has good application prospects.

The invention discloses an immunoregulation microsphere preparation targeting tumor-associated macrophages (TAMs) as well as a preparation method and application of the immunoregulation microsphere preparation. The microsphere preparation is a double-targeting preparation binding macrophage targeting peptide and B cell lymphoma 6 (Bcl6) factor inhibiting peptide; and the microsphere preparation isprepared as follows: the Bcl6 factor inhibiting peptide is supported on a liposome to obtain a Bcl6 factor inhibiting peptide-liposome solution; and then, the Bcl6 factor inhibiting peptide-liposomesolution is bound with the macrophage targeting peptide. The macrophage targeting peptide and an intracellular signal molecule inhibitor are bound for the first time and supported on nano microspheresto prepare the immune preparation directionally transferred to a tumor microenvironment. Besides, the preparation is found to be able to significantly reduce the proportion and number of macrophagesin lung cancer tumor and reverse stem cell-like phenotypes of the macrophages for the first time, and the preparation can significantly inhibit the size and weight of the lung cancer tumor and has a remarkable antitumor function.

The invention relates to a preparation method and application of a fluorescent probe for macrophage tracing. The probe chemically cross-links carboxymethyl dextran with lysine, one of the essential amino acids for human body, to form uniform and stable dextran. Glycan cross-linked nanoparticles, on which small imaging molecules such as indocyanine green were covalently linked, constructed nanoparticles with both macrophage targeting and imaging capabilities. The probe prepared by the invention has better macrophage targeting ability and higher biological safety.

The invention designs and synthesizes a graft polymer of mannan and phosphatidylethanolamine (PE) and the polymer is used as ligand and applied to the surface modification of nanocarrier. The invention belongs to the chemical technical field and is characterized in that the hydrophilic site of glycosyl, the hydrophobic site of lipid radical and the negative mannan ligand are prepared by chemosynthetic method with simple synthesis method and high yield; the products are applicable to the surface modification of nanocarrier of gene with positive ions of various carrier materials; because the mannan targets the mannose receptor on the surface of macrophage, the modified nanocarrier can react on the murine macrophage with low cytotoxicity, thus realizing the targeting transfection of macrophage of nanocarrier. The targeted graft polymer used as ligand is applicable to the surface modification of nanocarrier of gene with positive ions.

The invention belongs to the field of biotechnology, and relates to the new application of exosomes derived from mesenchymal stem cells, in particular to the construction of adipose-derived mesenchymal stem cells modified by lncRNA MALAT1 and the preparation of exosomes, and its application in the preparation of biological preparations for liver disease treatment and Application of liver macrophage targeting biologics. The idea of ​​the present invention is to construct MALAT1-modified AMSC, prepare its exosomes, and clarify the regulatory advantages of AMSC-MALAT1-Exo on the functional phenotype of macrophages at the cellular level through ELISA, WB, flow cytometry and other experiments, and through in vivo The experiment clarified the curative effect of MALAT1-modified AMSC-derived exosomes on liver diseases and its role in regulating the functional phenotype of liver macrophages. And further prepare liver macrophage targeting biological preparations or drugs with improved curative effect.