85 results about "Bile Juice" patented technology

The invention relates to the technical field of health wine preparation, and discloses a multi-effect synergistic enhanced bear gall body-building wine compound preparation process which comprises the following steps: taking sorghum, corn and fructus lycii as fermentation base materials, and carrying out double-strain synergistic fermentation by adopting saccharomyces cerevisiae and lactobacillus plantarum; adding fresh ursodesoxycholic acid during fermentation, carrying out in-situ enzymatic debinding conversion by using a compound enzyme system generated by a fermentation system, and converting the bound bile acid into free ursodesoxycholic acid; after fermentation, distilling to obtain raw wine; a molecularly imprinted polymer prepared by taking a wolfberry polyphenol key monomer as a template molecule is adopted to carry out targeted anchoring refining on raw wine, selective capture and enrichment of wolfberry polyphenol are carried out, and finally aging is carried out to obtain a finished product. According to the method disclosed by the invention, upstream double-strain fermentation in-situ enzymatic conversion and downstream molecular imprinting targeted refining are systematically integrated, so that the technical contradictions that the conversion rate of combined bile acid is low and a large amount of polyphenol functional components are synchronously lost during purification and impurity removal in a traditional process are solved.

The invention provides rhizoma alismatis purified polysaccharide as well as a preparation method, an identification method and application thereof, the rhizoma alismatis purified polysaccharide is a functional component with a novel structure in a traditional Chinese medicine rhizoma alismatis water extract obtained by further extraction and purification, the administration efficiency is effectively improved, and the pharmacodynamic substances are clarified; in-vivo and in-vitro experiments show that the polysaccharide can improve the antioxidant capacity of the liver and relieve inflammatory response; synthesis and metabolism of liver bile acid can be adjusted, cholestasis of the liver is relieved, and bile acid circulation is promoted so as to relieve acute liver injury caused by acetaminophen; the effect of promoting the recovery of liver injury patients is obvious; and the compound also can be used as prebiotics to improve the homeostasis of intestinal microorganisms and promote the synthesis of short-chain fatty acids so as to have beneficial effects on the liver.

This invention relates to a traditional Chinese medicine combination for treating intrahepatic cholestasis, which is made from the following raw materials in parts by weight: Artemisia capillaris and Glycyrrhiza uralensis 5:1 and 3:1. This invention also provides the preparation of the above-mentioned medicine and its use in treating intrahepatic cholestasis. Its advantages are: the traditional Chinese medicine composition of this invention (Artemisia capillaris and Glycyrrhiza uralensis decoction) has a significant ameliorative effect on intrahepatic cholestasis (non-obstructive type) induced by a 1% CA-containing diet in mice. Artemisia capillaris and Glycyrrhiza uralensis decoction can alleviate weight loss in mice and improve liver necrosis; significantly reduce serum ALT and ALP levels in mice; reduce the content of total bile acids in the liver and decrease the proportion of toxic bile acid taurine deoxycholic acid (TDCA) in the bile acid pool; upregulate liver BSEP protein expression to promote bile acid efflux; and downregulate liver NLRP3 protein expression to alleviate liver necrosis.

This invention relates to the use of a recombinant protein ANG in the preparation of medicaments for treating cholestatic liver disease. Specifically, this invention provides the use of the angiopoietin ANG gene, or its protein, or its promoter, for the preparation of medicaments, compositions, or formulations for the prevention, improvement, and / or treatment of cholestatic liver disease. It is particularly effective in treating primary biliary cholangitis and primary sclerosing cholangitis.

The invention discloses a probiotic with the characteristic of high yield of bile salt hydrolase and application of the probiotic in a preparation for preventing or improving cholestatic jaundice, and is characterized in that the probiotic is at least one of a bifidobacterium longum subsp. Infantis QS033 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35879 and a lactobacillus delbrueckii subsp. Lactis QS021 strain with the preservation number of CGMCC No.35880; according to the present invention, the Chukrasone A has the application of the Chukrasone A in the preparation of the product for preventing, relieving or regulating the cholestatic jaundice, and has the advantages of effective degradation of sodium glycocholate, sodium glycodeoxycholate, sodium glycochenodeoxycholate and sodium glycoursodeoxycholate, effective regulation of bile acid metabolism disorder in the cholestatic jaundice, and improvement of the cholestatic jaundice.

The application relates to the embedding technical field, and particularly discloses a double emulsion gel bead embedding beta-carotene and probiotics and a preparation method thereof. The double emulsion gel bead comprises a composite layer, an oil phase and an inner water phase from outside to inside. The composite layer comprises an outer water phase and calcium alginate which are fused with each other. The outer water phase comprises a product after carrageenan and sodium caseinate are subjected to a Maillard reaction. The oil phase comprises beta-carotene, vegetable oil and a food emulsifier. The inner water phase comprises probiotics. In the gel bead, the beta-carotene and the probiotics are simultaneously embedded in the double emulsion gel bead, the calcium alginate hydrogel shell starts to decompose when entering the intestinal tract, then the double-layer emulsion is slowly decomposed after being subjected to chemical digestion of pancreatic juice, bile and small intestinal juice and mechanical digestion of the small intestine in the small intestine, and the beta-carotene and the probiotics are gradually released, the beta-carotene is converted into vitamin A in the intestinal tract and is absorbed, and the probiotics are delivered to the colon to play a probiotic effect.

The present application provides a compound of formula (I), or an optical isomer thereof, a pharmaceutically acceptable salt, a solvated form, or a prodrug. The compound may modulate NTCP function for the prevention and / or treatment of HBV / HDV and hepatitis virus-related diseases, bile acid-related cholestasis disorders, metabolic disorders, and / or liver diseases. Additionally, the present application relates to a pharmaceutical composition comprising such a compound and a method for modulating NTCP.

The present invention relates to a molecular association of a bile acid or a bile salt and a composition containing the same, and more specifically, to a skin-permeable formulation of deoxycholic acid, a lipolytic pharmacological agent, which has been developed as an external preparation rather than a conventional injection, and which dramatically improves patient compliance.

The present application belongs to the technical field of bear gall powder production, and provides a bear gall powder production method and a freeze-drying device. The method comprises the following steps: taking bear bile and filtering, obtaining a filtered bile solution after filtering; placing the filtered bile solution in a condition of-40 DEG C to-45 DEG C for pre-freezing treatment for 1.5 to 2.5 hours, keeping for 1.5 to 2.0 hours after pre-freezing; increasing the temperature to-10 DEG C to-15 DEG C, keeping for 1.5 to 2.5 hours; then controlling the temperature to increase in stages, keeping in stages, increasing the temperature to 37 DEG C to 40 DEG C and keeping, obtaining dry materials; sieving the dry materials, obtaining bear gall powder. The method can effectively reduce energy consumption, shorten processing time, and obtain bear gall powder with less impurities, low moisture, high purity and stable quality. The device has more uniform heat conduction, more sensitive temperature regulation, and can better meet the temperature increasing, temperature decreasing, keeping and other regulation and control requirements of the bear gall powder freeze-drying process.

The dehydrator for gall bladder processing comprises a base, a first motor is connected to the left side of the top end of the base through a screw, one end of a first driving rod is installed at the output end of the lower surface of the first motor, and the other end of the first driving rod extends into an inner cavity of the base; the bottom end of the first driving rod is rotationally connected to the inner wall of the base through a bearing, and a first driving wheel is installed on the outer wall of the first driving rod. The device has a self-cleaning function, residual bile and other substances in the box body can be rapidly and thoroughly removed after each time of processing is completed, cross contamination of materials during processing of different batches of gall bladders is effectively avoided, it is ensured that each batch of gall bladders processed products are higher in purity and more stable in quality, manual cleaning is not needed, operation is easy and convenient, and time and labor are saved; the whole processing efficiency is greatly improved, the sanitary standard is met, the use is convenient, the practicability is high, and the use requirements on the existing market are met.

The utility model provides a hepatopancreatic ampulla metabolism device which comprises a main drainage tube, a branch drainage tube is connected to the main drainage tube, a medicine release mechanism is arranged at the position, close to the inlet end, of the tube wall of the main drainage tube, and protrusions are arranged on the tube wall of the main drainage tube. The main drainage tube and the branch drainage tube are arranged to drain bile and pancreatic juice at the hepatopancreatic ampulla, discharge blocking of the bile and the pancreatic juice caused by abnormal metabolism at the hepatopancreatic ampulla is avoided, the medicine release mechanism is arranged to release medicine to the hepatopancreatic ampulla, inflammation caused by abnormal metabolism at the hepatopancreatic ampulla is solved, and the hepatopancreatic ampulla is prevented from being damaged by abnormal metabolism at the hepatopancreatic ampulla. And by arranging the bulges, bile and pancreatic juice excreted by drainage are uniformly mixed.

Provided herein are formulations for use in enteral tube feeding, that contain one or more whole food-based ingredients, mineral nutrients, small molecules, and / or large molecules, the combination of which is effective to bind bile acids when administered to a subject in need thereof. The combination of crafted ingredients for tube feeding reduce symptoms of gastroesophageal reflux, vomiting, abdominal pain, irregular stooling (diarrhea or constipation) and to reduce organ damage including pneumonia, pharyngitis, aspiration, esophagitis, gastritis and enteritis.

This invention relates to the field of feed additive technology, specifically to a feed additive that promotes digestion and absorption and its preparation process. The preparation process of a feed additive that promotes digestion and absorption includes: preparing antioxidant-modified chicken fat; preparing an extract; preparing a composite oil phase; and preparing the feed additive. In this invention, the extract of Shenqu (a type of fermented wheat) can directly compensate for insufficient endogenous enzyme secretion in laying hens, rapidly decomposing starch in feed and chicken fat in plant proteins, laying the foundation for subsequent absorption. The extract of Zhishi (a type of immature bitter orange) accelerates gastrointestinal motility and digesta emptying, while also promoting bile and pancreatic juice secretion, further improving the digestibility and utilization rate of fat-soluble components in laying hens. The extract of pine pollen can repair the intestinal mucosa, increase intestinal villus height, increase the nutrient absorption area, and regulate the intestinal microecology, reducing nutrient loss and intestinal stress. The combined use of these three ingredients synergistically promotes the digestive and absorptive capacity of laying hens.

This invention discloses a method for fabricating a memristor based on a bile functional layer, belonging to the field of bioelectronic device fabrication and in vitro diagnostics of biliary tract diseases. The method sequentially completes FTO substrate pretreatment, bile functional layer preparation, Ag-on-electrode deposition, and memristor detection array fabrication. First, the FTO substrate is physically cleaned and plasma-activated. Then, a 240-260 nm bile functional layer is prepared. Subsequently, an Ag-on-electrode is deposited, and a 5-15 nm Ag atom embedding transition region is constructed. Finally, a 2×2 array is arranged and passivated for isolation. The process parameters of each step are synergistically matched to optimize the interface between the substrate, functional layer, and electrode, fully preserving bile pathological information. The resulting Ag / Bile / FTO structure memristor exhibits high electrical stability and shows significant differences in electrical response to bile samples from normal, gallstone, and gallbladder cancer patients. It enables accurate and rapid in vitro identification of biliary tract diseases, and the process is simple, low-cost, and suitable for clinical application and large-scale production.

The invention discloses an extraction method of 5 alpha-carp cholesteryl sulfate, and belongs to the technical field of extraction and purification of bile acid active substances. The extraction method of 5 alpha-carp cholesteryl sulfate comprises the following steps: (1) carrying out freeze drying or rotary evaporation concentration on fresh fish bile to obtain a crude product; (2) mixing and dissolving the crude product obtained in the step (1) with hot methanol at 50-80 DEG C according to a mass ratio of the crude product to the methanol of 1: (5-10), carrying out suction filtration while the crude product is hot, and collecting filtrate; and (3) ethyl acetate is added into the filtrate obtained in the step (2), the mass ratio of the methyl alcohol to the ethyl acetate is 1: (1-2), standing is conducted, a solid is separated out, and the obtained solid is the 5 alpha-carp cholesteryl sulfate. The extraction method disclosed by the invention is simple and convenient, high in yield, high in product quality and low in cost.

The present invention relates to imidazo[1,2-a]pyridine and [1,2,4]triazolo[1,5-a]pyridine derivatives of Formula (I): or a salt or prodrug thereof. These compounds are TLR9 inhibitors useful for the treatment, prevention or slowing of a fibrotic disease, such as liver fibrosis, kidney fibrosis, biliary fibrosis or pancreatic fibrosis, nonalcoholic steatohepatitis (NASH), nonalcoholic fatty liver disease (NAFLD), chronic kidney disease, diabetic nephropathy, primary sclerosing cholangitis (PSC) or primary biliary cirrhosis (PBC), or idiopathic pulmonary fibrosis (IPF).

The invention relates to the technical field of food processing and biology, discloses a genetic analysis bear gall body-building wine liver and stomach maintenance and immunity enhancement process, and solves the problems of unstable quality, low component dissolution rate and fuzzy efficacy orientation caused by fluctuation of raw material components and single soaking process in the prior art. Comprising the following steps: carrying out biochemical fingerprint analysis on fresh ursodesoxycholic acid and fructus lycii, and carrying out efficacy typing according to the contents of ursodesoxycholic acid, lycium barbarum polysaccharide, betaine and the like; carrying out targeted bio-enzyme pretreatment on the specific Chinese wolfberry fruits according to the typing result; raw materials of specific types are subjected to efficacy coupling combination, stepped dynamic alcoholysis soaking is adopted, and components with different dissolution characteristics are fully extracted through low-alcohol pre-dissolution and gradient alcohol rising steps; and finally, filtering, harmonizing and alcoholizing. According to the scheme, raw material standardization and process precision are combined, so that the stability of product quality is ensured, the transfer rate of functional components is increased, and liver and stomach nourishing or immunity enhancing products with definite efficacy can be directionally prepared.

The invention belongs to the technical field of health care products, and discloses a preparation method and application of lemon vesicle embedded active probiotics. The invention discloses lemon vesicle embedded active probiotics which comprise probiotics and lemon vesicles coating the surfaces of the probiotics, and the lemon vesicles are extracellular vesicles from lemons. The lemon vesicles are used for performing single-cell embedding on the active probiotics, so that the lemon vesicles can resist degradation of gastric acid and bile, the survival rate and colonization rate of the active probiotics in the intestinal tract through the stomach are increased, the balance of the active probiotics in regulating the intestinal microecology is promoted, various functions of the active probiotics are efficiently realized, and the effects of improving the intestinal tract microecology and improving the intestinal tract microecology are achieved. Meanwhile, the natural active ingredients of the lemon vesicles can synergistically reduce inflammatory factors of intestinal tracts. The lemon vesicles provide a brand new probiotic delivery technology for active probiotics, and a new development strategy is brought to the oral probiotic industry in the future.

The present invention relates to a pharmaceutical composition comprising malalixib or a pharmaceutically acceptable salt thereof as an active ingredient. The invention also relates to the use of said pharmaceutical composition as a solid dose medicament. The invention also relates to the use of the pharmaceutical compositions and solid doses described herein for the treatment of cholestatic pruritus and cholestatic liver disease.

The invention provides a pharmaceutical composition for treating cholestatic liver injury, and the pharmaceutical composition contains an effective amount of Ferulic acid and Wogonin. The application creatively focuses on two key links in the cholestatic liver injury process, namely (1) bile acid excretion and (2) intestinal mucous barrier protection, a synergistic component of Ferulic acid and wogonin is provided, and the optimal ratio of Ferulic acid and wogonin is verified through an experimental system. Experiments prove that the cholestatic liver injury can be effectively improved by combined application of the Ferric acid and the Wogonin which are derived from traditional Chinese medicines; in addition, the effect of the medicine composition is obviously superior to the effect of independently using the Ferric acid or the Wogonin. Wide application prospects are realized.

The application discloses a capsule for assisting in protecting chemical liver injury and a preparation method thereof, and belongs to the technical field of health-care food. The capsule is composed of curcuma longa extract 100g, silybum marianum extract 70g, schisandra chinensis extract 60g, microcrystalline cellulose 109.5g, silicon dioxide 7g and magnesium stearate 3.5g, and 1000 capsules are taken as a unit. The curcuma longa extract contains curcumin, the content of which is greater than or equal to 10% (10:1), the silybum marianum extract contains silybin, the content of which is greater than or equal to 20% (25:1), and the schisandra chinensis extract contains schisandrin, the content of which is greater than or equal to 1.5% (12.5:1). The three kinds of plant extracts are scientifically compounded, and the effects of antioxidation, anti-inflammation, liver cell membrane protection and bile secretion regulation are synergistically exerted. Through systematic screening of the types and dosages of the filling agent, the flow agent and the lubricant, the technical problems of poor flowability of the mixed plant extract powder, large filling difference and easy jamming are solved.

The application belongs to the technical field of biomedicine, and discloses a method for constructing a Channa argus bile stasis model. The application discloses application of rifampicin or a pharmaceutically acceptable salt thereof in construction of a fish liver injury model. The application discloses, for the first time, that the antibiotic rifampicin widely used in aquaculture is used to construct a fish liver injury model. The model is closer to the real scene of the use of a large amount of drugs when bacterial diseases break out in production practice. The injury type of the fish liver injury model is clearly directed to bile stasis, which fills the research blank of the disease model of the fish, and is different from common hepatocyte necrosis or fatty liver models.

The invention belongs to the technical field of pet food, and particularly relates to a nutritional composition, puffed pet food and a preparation method of the puffed pet food. The nutritional composition comprises an inner core and a coating layer coating the surface of the inner core, wherein the coating layer sequentially comprises a first coating layer, a second coating layer and a third coating layer from outside to inside; the inner core comprises a nutritional additive; the first coating layer comprises a biomolecular polymer, the second coating layer comprises a lipid substance, and the third coating layer comprises a protein. Through the structural design of the microencapsulated multilayer coating, the loss of the nutritional additive in the extrusion process and the loss of the nutritional additive in gastric acid and bile can be reduced, and the nutritional additive can be accurately released in the intestinal tract at a fixed point, so that the dosage of raw materials is reduced, and the cost is reduced.

The invention discloses a traditional Chinese medicine composition for treating intestinal tract diseases, which is prepared from the following traditional Chinese medicines in parts by weight: 8-15 parts of selfheal, 20-40 parts of nacre mother of pearl, 5-10 parts of radix bupleuri, 3-8 parts of fried fructus aurantii, 3-8 parts of rhizoma cyperi, 6-12 parts of inula flower and 5-9 parts of pericarpium citri reticulatae. The traditional Chinese medicine composition can be used for pertinently treating liver and gall stagnated heat, stomach qi reversal type bile reflux gastritis or gastroesophageal reflux disease, has the specific treatment effects of relieving inflammation, inhibiting local tissue angiogenesis and preventing precancerous lesions and / or canceration, and has good clinical significance.

The invention discloses a 7 alpha-hydroxysteroid dehydrogenase (7 alpha-HSDH) gene. The nucleotide sequence of the 7 alpha-HSDH gene is shown as SEQ ID No: 1. The method comprises the following steps: jointly transforming bacillus subtilis by using a modified 7alpha-HSDH gene, a 7beta-HSDH gene and a GDH gene to obtain recombinant bacillus subtilis; the recombinant bacillus subtilis is used for directional transformation of TCDCA in poultry bile, the recombinant bacillus subtilis has outstanding advantages in the aspect of green manufacturing of artificial bear gall powder, the expression quantity and activity of key enzymes are regulated and controlled through a genetic engineering technology, NADPH coenzyme circulation coupled with the key enzymes is enhanced, efficient and stable directional transformation from TCDCA to TUDCA can be achieved, and the recombinant bacillus subtilis can be used for preparing the artificial bear gall powder. Therefore, comprehensive utilization of resources is achieved, and the requirements of the medicine market are met more effectively.

Provided herein are methods for treating cholestasis in a subject having a liver disease. The method includes administering to the subject an Apical Sodium-dependent Bile Acid Transporter (ASBTI). More specifically, the present invention relates to methods for treating cholestasis in a subject where the method includes administering an ASBTI to a subject at a dose of at least 10 μg / kg / day.

The utility model relates to medical instrument technical field especially, and it is a kind of covered stent and implantation system.This covered stent includes support framework and film, wherein: support framework is the hollowed out cylinder structure of peripheral surface, it can be radially compressed and expand;Film is coated on part support framework, and drainage mesh is formed at the region of support framework not being coated by film.Due to film being coated on part support framework, drainage mesh is formed at the region of support framework not being coated by film, bile of gallbladder, bile in common hepatic duct and pancreatic juice in pancreatic duct can enter the inside of covered stent through drainage mesh and reach duodenal papilla, thereby avoiding the phenomenon that bile and pancreatic juice cannot flow out from common bile duct and reach duodenal papilla due to common bile duct being pressed by stent, and simultaneously, a plastic stent does not need to be additionally implanted in common bile duct to help common bile duct drainage, to achieve the purpose of reducing operation cost and operation complexity.

This invention provides a combination of bile protein biomarkers for predicting liver metastasis of pancreatic cancer and its application. The combination of bile protein biomarkers includes at least MMP9, PRTN3, and S100A12. This application transforms bile, previously considered biological waste, into a high-value diagnostic sample. Based on the differences in bile proteins among patients with benign disease, patients with pancreatic cancer without liver metastasis, and patients with pancreatic cancer with liver metastasis, four optimal biomarker combinations are screened for the first time to establish a method for predicting the risk of pancreatic cancer liver metastasis for early preoperative assessment of pancreatic cancer liver metastasis risk.

The present application relates to a kind of traditional Chinese medicine composition for treating calculus, raw material is: walnut kernel 25-35 parts, black fungus 10-20 parts, giant knotweed 15-25 parts, powder 25-35 parts of yam, sea gold sand 25-35 parts, 10-20 parts of yujin, 25-35 parts of huashi grass, 25-35 parts of shaddock, 10-20 parts of goose tube stone, 25-35 parts of yiwren, 10-20 parts of magnolia officinalis, 25-35 parts of xuanming powder, 10-20 parts of rhubarb, 10-20 parts of plantago, 10-20 parts of bupleurum, 10-20 parts of small chrysanthemum, 5-10 parts of cumin, 10-20 parts of Sichuan donkey's hide, 5-10 parts of raw licorice.The present application has the effect of clearing heat and promoting bile secretion, dissolving stone and stopping bleeding cooling blood, through the concept of dissolving stone and expelling stone, large stone is small, small stone is small, gallstones are discharged with bile into intestine, kidney stones, ureter and bladder stones are discharged with urine, adjust liver, gallbladder, kidney and other qi stagnation, and be supplemented with constitution conditioning syndrome differentiation treatment, it has good curative effect on various stones of liver, gallbladder, kidney and urinary tract, and it is both the treatment of principle and secondary, relieve the suffering of the majority of patients.

The invention relates to the technical field of bioengineering, in particular to a membrane-assisted extraction process for extracting bile acid from animal viscera, animal bile is subjected to enzymolysis, and then the bile acid is extracted through the steps of ultrafiltration membrane treatment, composite membrane treatment, acidification, recrystallization and the like. Meanwhile, the usage amount of organic solvents can be reduced, and environmental protection is achieved.