136 results about "Bile Juice" patented technology

The invention relates to the technical field of health wine preparation, and discloses a multi-effect synergistic enhanced bear gall body-building wine compound preparation process which comprises the following steps: taking sorghum, corn and fructus lycii as fermentation base materials, and carrying out double-strain synergistic fermentation by adopting saccharomyces cerevisiae and lactobacillus plantarum; adding fresh ursodesoxycholic acid during fermentation, carrying out in-situ enzymatic debinding conversion by using a compound enzyme system generated by a fermentation system, and converting the bound bile acid into free ursodesoxycholic acid; after fermentation, distilling to obtain raw wine; a molecularly imprinted polymer prepared by taking a wolfberry polyphenol key monomer as a template molecule is adopted to carry out targeted anchoring refining on raw wine, selective capture and enrichment of wolfberry polyphenol are carried out, and finally aging is carried out to obtain a finished product. According to the method disclosed by the invention, upstream double-strain fermentation in-situ enzymatic conversion and downstream molecular imprinting targeted refining are systematically integrated, so that the technical contradictions that the conversion rate of combined bile acid is low and a large amount of polyphenol functional components are synchronously lost during purification and impurity removal in a traditional process are solved.

To provide cellular uptake.SOLUTION: The invention provides a bile acid or a pharmaceutically acceptable salt thereof for use in a method for treating the human or animal body, the method comprises administering to the human or animal body a therapeutic compound, where: a. the bile acid is chenodeoxycholic acid or deoxycholic acid; b. optionally, the bile acid is employed in conjunction with EDTA; c. in the method, the bile acid or salt thereof and EDTA are used to enable or enhance intracellular uptake of the therapeutic compound.SELECTED DRAWING: None

The invention provides rhizoma alismatis purified polysaccharide as well as a preparation method, an identification method and application thereof, the rhizoma alismatis purified polysaccharide is a functional component with a novel structure in a traditional Chinese medicine rhizoma alismatis water extract obtained by further extraction and purification, the administration efficiency is effectively improved, and the pharmacodynamic substances are clarified; in-vivo and in-vitro experiments show that the polysaccharide can improve the antioxidant capacity of the liver and relieve inflammatory response; synthesis and metabolism of liver bile acid can be adjusted, cholestasis of the liver is relieved, and bile acid circulation is promoted so as to relieve acute liver injury caused by acetaminophen; the effect of promoting the recovery of liver injury patients is obvious; and the compound also can be used as prebiotics to improve the homeostasis of intestinal microorganisms and promote the synthesis of short-chain fatty acids so as to have beneficial effects on the liver.

This invention relates to a traditional Chinese medicine combination for treating intrahepatic cholestasis, which is made from the following raw materials in parts by weight: Artemisia capillaris and Glycyrrhiza uralensis 5:1 and 3:1. This invention also provides the preparation of the above-mentioned medicine and its use in treating intrahepatic cholestasis. Its advantages are: the traditional Chinese medicine composition of this invention (Artemisia capillaris and Glycyrrhiza uralensis decoction) has a significant ameliorative effect on intrahepatic cholestasis (non-obstructive type) induced by a 1% CA-containing diet in mice. Artemisia capillaris and Glycyrrhiza uralensis decoction can alleviate weight loss in mice and improve liver necrosis; significantly reduce serum ALT and ALP levels in mice; reduce the content of total bile acids in the liver and decrease the proportion of toxic bile acid taurine deoxycholic acid (TDCA) in the bile acid pool; upregulate liver BSEP protein expression to promote bile acid efflux; and downregulate liver NLRP3 protein expression to alleviate liver necrosis.

The invention provides a liver protection composition as well as a preparation method, functional food and application thereof, and relates to the technical field of food processing. The liver protection composition comprises the following raw materials: dandelion concentrated solution, red ginseng extract and hovenia dulcis thunb. The liver-protecting composition prepared by the invention is remarkable in liver-protecting effect and high in comprehensiveness, can realize the liver-protecting effect from multiple aspects of oxidation resistance, inflammation resistance, biliary secretion promotion and the like, is high in bioavailability, completely selects natural plants as raw materials, and does not contain artificial additives, preservatives or sweeteners.

This invention relates to the use of a recombinant protein ANG in the preparation of medicaments for treating cholestatic liver disease. Specifically, this invention provides the use of the angiopoietin ANG gene, or its protein, or its promoter, for the preparation of medicaments, compositions, or formulations for the prevention, improvement, and / or treatment of cholestatic liver disease. It is particularly effective in treating primary biliary cholangitis and primary sclerosing cholangitis.

The invention discloses a probiotic with the characteristic of high yield of bile salt hydrolase and application of the probiotic in a preparation for preventing or improving cholestatic jaundice, and is characterized in that the probiotic is at least one of a bifidobacterium longum subsp. Infantis QS033 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35879 and a lactobacillus delbrueckii subsp. Lactis QS021 strain with the preservation number of CGMCC No.35880; according to the present invention, the Chukrasone A has the application of the Chukrasone A in the preparation of the product for preventing, relieving or regulating the cholestatic jaundice, and has the advantages of effective degradation of sodium glycocholate, sodium glycodeoxycholate, sodium glycochenodeoxycholate and sodium glycoursodeoxycholate, effective regulation of bile acid metabolism disorder in the cholestatic jaundice, and improvement of the cholestatic jaundice.

The invention provides a high-fidelity calculus bovis and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. According to the technical scheme provided by the invention, the high-fidelity calculus bovis is accurately prepared by taking the mean value of the contents of various components of the natural calculus bovis as a reference and accurately measuring the contents of various components in the raw materials, fully utilizing the original components of the ox bile or ox gall powder, not adding other non-calculus bovis components and combining the existence forms of the components. The problems that existing artificial calculus bovis is greatly different from natural calculus bovis in efficacy, and toxic and side effects are relatively high are solved. The shape, the forming process and the components of the product are highly similar to those of natural calculus bovis; the medicine effect of the calculus bovis is improved, and the production cost is reduced.

The invention relates to the technical field of biological pharmacy, in particular to a method for extracting bile acid from pig bile, which comprises the following steps: (1) taking the pig bile, adjusting the pH value of the pig bile to be alkaline, heating the pig bile to 80-90 DEG C, stirring at constant temperature for 25-35 minutes, then cooling to room temperature, and adjusting the pH value of the system to be neutral to obtain pretreated pig bile; (2) filtering the pretreated pig bile in the step (1) by using a hyperbranched polymer film, concentrating filtrate under reduced pressure, adding an ethanol solution, heating to reflux, stirring for 25-35 minutes, and then filtering to obtain a crude bile acid product; and (3) adding a hydrochloric acid solution into the crude bile acid product in the step (2), adjusting the pH value to 2-3, centrifuging, filtering, washing and drying the obtained solid, adding ethyl acetate, heating to reflux until the solid is dissolved, cooling to room temperature, stirring for 2-4 hours, recrystallizing, separating and drying to obtain the bile acid. The extraction method is simple, and the extraction purity is high.

The present invention relates to the technical field of fowl bile processing, and in particular to a method for detecting the bile acid content in fowl bile based on turbidity, which comprises the following steps: adjusting a solution containing bound bile acid to alkaline to obtain a suspension; and then measuring the OD value of the suspension. 600 This solution can solve the technical problems of long time and high cost in detecting bile acid content in poultry bile. When the purchased poultry bile raw materials arrive at the inspection site, this method can be used to conduct a preliminary test on the bile acid content in the poultry bile, and complete the on-site inspection work. Unqualified products with too low bile acid content can be returned on the spot, which greatly saves time and cost. This solution can be applied to practical operations such as the quality inspection of poultry bile, the research and development and production of poultry bile powder and artificial bear bile powder.

The application relates to the embedding technical field, and particularly discloses a double emulsion gel bead embedding beta-carotene and probiotics and a preparation method thereof. The double emulsion gel bead comprises a composite layer, an oil phase and an inner water phase from outside to inside. The composite layer comprises an outer water phase and calcium alginate which are fused with each other. The outer water phase comprises a product after carrageenan and sodium caseinate are subjected to a Maillard reaction. The oil phase comprises beta-carotene, vegetable oil and a food emulsifier. The inner water phase comprises probiotics. In the gel bead, the beta-carotene and the probiotics are simultaneously embedded in the double emulsion gel bead, the calcium alginate hydrogel shell starts to decompose when entering the intestinal tract, then the double-layer emulsion is slowly decomposed after being subjected to chemical digestion of pancreatic juice, bile and small intestinal juice and mechanical digestion of the small intestine in the small intestine, and the beta-carotene and the probiotics are gradually released, the beta-carotene is converted into vitamin A in the intestinal tract and is absorbed, and the probiotics are delivered to the colon to play a probiotic effect.

The present application provides a compound of formula (I), or an optical isomer thereof, a pharmaceutically acceptable salt, a solvated form, or a prodrug. The compound may modulate NTCP function for the prevention and / or treatment of HBV / HDV and hepatitis virus-related diseases, bile acid-related cholestasis disorders, metabolic disorders, and / or liver diseases. Additionally, the present application relates to a pharmaceutical composition comprising such a compound and a method for modulating NTCP.

The present invention relates to a molecular association of a bile acid or a bile salt and a composition containing the same, and more specifically, to a skin-permeable formulation of deoxycholic acid, a lipolytic pharmacological agent, which has been developed as an external preparation rather than a conventional injection, and which dramatically improves patient compliance.

The present application belongs to the technical field of bear gall powder production, and provides a bear gall powder production method and a freeze-drying device. The method comprises the following steps: taking bear bile and filtering, obtaining a filtered bile solution after filtering; placing the filtered bile solution in a condition of-40 DEG C to-45 DEG C for pre-freezing treatment for 1.5 to 2.5 hours, keeping for 1.5 to 2.0 hours after pre-freezing; increasing the temperature to-10 DEG C to-15 DEG C, keeping for 1.5 to 2.5 hours; then controlling the temperature to increase in stages, keeping in stages, increasing the temperature to 37 DEG C to 40 DEG C and keeping, obtaining dry materials; sieving the dry materials, obtaining bear gall powder. The method can effectively reduce energy consumption, shorten processing time, and obtain bear gall powder with less impurities, low moisture, high purity and stable quality. The device has more uniform heat conduction, more sensitive temperature regulation, and can better meet the temperature increasing, temperature decreasing, keeping and other regulation and control requirements of the bear gall powder freeze-drying process.

The dehydrator for gall bladder processing comprises a base, a first motor is connected to the left side of the top end of the base through a screw, one end of a first driving rod is installed at the output end of the lower surface of the first motor, and the other end of the first driving rod extends into an inner cavity of the base; the bottom end of the first driving rod is rotationally connected to the inner wall of the base through a bearing, and a first driving wheel is installed on the outer wall of the first driving rod. The device has a self-cleaning function, residual bile and other substances in the box body can be rapidly and thoroughly removed after each time of processing is completed, cross contamination of materials during processing of different batches of gall bladders is effectively avoided, it is ensured that each batch of gall bladders processed products are higher in purity and more stable in quality, manual cleaning is not needed, operation is easy and convenient, and time and labor are saved; the whole processing efficiency is greatly improved, the sanitary standard is met, the use is convenient, the practicability is high, and the use requirements on the existing market are met.

The invention discloses an oxgall-tolerant in-vitro calculus bovis culture transformation strain and application thereof, and belongs to the technical field of calculus bovis culture. The strain is obtained by separating and purifying fresh ox bile, is identified to belong to escherichia coli, has excellent ox bile tolerance, has the characteristics of beta-glucuronidase, high bezoar conversion rate and high safety, and can be used in the production of in-vitro artificial cultivation of bezoar. The strain is a brand new strain for in-vitro calculus bovis transformation, not only enriches a strain resource library of calculus bovis transformation bacteria, but also provides a new thought for deep research of subsequent in-vitro calculus bovis culture.

The utility model provides a hepatopancreatic ampulla metabolism device which comprises a main drainage tube, a branch drainage tube is connected to the main drainage tube, a medicine release mechanism is arranged at the position, close to the inlet end, of the tube wall of the main drainage tube, and protrusions are arranged on the tube wall of the main drainage tube. The main drainage tube and the branch drainage tube are arranged to drain bile and pancreatic juice at the hepatopancreatic ampulla, discharge blocking of the bile and the pancreatic juice caused by abnormal metabolism at the hepatopancreatic ampulla is avoided, the medicine release mechanism is arranged to release medicine to the hepatopancreatic ampulla, inflammation caused by abnormal metabolism at the hepatopancreatic ampulla is solved, and the hepatopancreatic ampulla is prevented from being damaged by abnormal metabolism at the hepatopancreatic ampulla. And by arranging the bulges, bile and pancreatic juice excreted by drainage are uniformly mixed.

The invention discloses a pet cat food formula and a making process thereof. The pet cat food formula comprises animal basic components: 25% of enzymolysis fresh chicken with bones, 30% of fresh chicken, 10% of fresh complete quail, 8% of fish scale-containing fresh salmon, 5% of fresh chicken liver, 4% of fresh chicken heart and 3% of fermented animal oil; the functional additives comprise 6% of functional fruit and vegetable groups, 2% of freeze-dried queen bee larva powder, 0.5% of propolis, 0.5% of wall-broken bee pollen, 2% of euphausia superba powder, 1% of ostrich liver powder, 1% of catgrass powder and 0.5% of eggshell membrane powder; nutritional additives: 2% of microencapsulated egg yolk powder and 0.2% of a probiotic post-spraying bag; and 0.3% of montmorillonite. According to the method, the meat with bones is pretreated through an enzymolysis technology, macromolecular protein and calcium are pre-decomposed, and the protein digestibility and the calcium bioavailability are remarkably improved; the fermented animal oil is decomposed into smaller molecules and can be directly absorbed without bile emulsification, so that the fat absorption rate is higher; the microencapsulation technology protects the activity of thermosensitive nutrients, and the overall nutrition utilization efficiency is greatly improved.

Provided herein are formulations for use in enteral tube feeding, that contain one or more whole food-based ingredients, mineral nutrients, small molecules, and / or large molecules, the combination of which is effective to bind bile acids when administered to a subject in need thereof. The combination of crafted ingredients for tube feeding reduce symptoms of gastroesophageal reflux, vomiting, abdominal pain, irregular stooling (diarrhea or constipation) and to reduce organ damage including pneumonia, pharyngitis, aspiration, esophagitis, gastritis and enteritis.

This invention relates to the field of feed additive technology, specifically to a feed additive that promotes digestion and absorption and its preparation process. The preparation process of a feed additive that promotes digestion and absorption includes: preparing antioxidant-modified chicken fat; preparing an extract; preparing a composite oil phase; and preparing the feed additive. In this invention, the extract of Shenqu (a type of fermented wheat) can directly compensate for insufficient endogenous enzyme secretion in laying hens, rapidly decomposing starch in feed and chicken fat in plant proteins, laying the foundation for subsequent absorption. The extract of Zhishi (a type of immature bitter orange) accelerates gastrointestinal motility and digesta emptying, while also promoting bile and pancreatic juice secretion, further improving the digestibility and utilization rate of fat-soluble components in laying hens. The extract of pine pollen can repair the intestinal mucosa, increase intestinal villus height, increase the nutrient absorption area, and regulate the intestinal microecology, reducing nutrient loss and intestinal stress. The combined use of these three ingredients synergistically promotes the digestive and absorptive capacity of laying hens.

This invention discloses a method for fabricating a memristor based on a bile functional layer, belonging to the field of bioelectronic device fabrication and in vitro diagnostics of biliary tract diseases. The method sequentially completes FTO substrate pretreatment, bile functional layer preparation, Ag-on-electrode deposition, and memristor detection array fabrication. First, the FTO substrate is physically cleaned and plasma-activated. Then, a 240-260 nm bile functional layer is prepared. Subsequently, an Ag-on-electrode is deposited, and a 5-15 nm Ag atom embedding transition region is constructed. Finally, a 2×2 array is arranged and passivated for isolation. The process parameters of each step are synergistically matched to optimize the interface between the substrate, functional layer, and electrode, fully preserving bile pathological information. The resulting Ag / Bile / FTO structure memristor exhibits high electrical stability and shows significant differences in electrical response to bile samples from normal, gallstone, and gallbladder cancer patients. It enables accurate and rapid in vitro identification of biliary tract diseases, and the process is simple, low-cost, and suitable for clinical application and large-scale production.

The invention discloses an extraction method of 5 alpha-carp cholesteryl sulfate, and belongs to the technical field of extraction and purification of bile acid active substances. The extraction method of 5 alpha-carp cholesteryl sulfate comprises the following steps: (1) carrying out freeze drying or rotary evaporation concentration on fresh fish bile to obtain a crude product; (2) mixing and dissolving the crude product obtained in the step (1) with hot methanol at 50-80 DEG C according to a mass ratio of the crude product to the methanol of 1: (5-10), carrying out suction filtration while the crude product is hot, and collecting filtrate; and (3) ethyl acetate is added into the filtrate obtained in the step (2), the mass ratio of the methyl alcohol to the ethyl acetate is 1: (1-2), standing is conducted, a solid is separated out, and the obtained solid is the 5 alpha-carp cholesteryl sulfate. The extraction method disclosed by the invention is simple and convenient, high in yield, high in product quality and low in cost.

The present invention relates to imidazo[1,2-a]pyridine and [1,2,4]triazolo[1,5-a]pyridine derivatives of Formula (I): or a salt or prodrug thereof. These compounds are TLR9 inhibitors useful for the treatment, prevention or slowing of a fibrotic disease, such as liver fibrosis, kidney fibrosis, biliary fibrosis or pancreatic fibrosis, nonalcoholic steatohepatitis (NASH), nonalcoholic fatty liver disease (NAFLD), chronic kidney disease, diabetic nephropathy, primary sclerosing cholangitis (PSC) or primary biliary cirrhosis (PBC), or idiopathic pulmonary fibrosis (IPF).

The invention relates to the technical field of food processing and biology, discloses a genetic analysis bear gall body-building wine liver and stomach maintenance and immunity enhancement process, and solves the problems of unstable quality, low component dissolution rate and fuzzy efficacy orientation caused by fluctuation of raw material components and single soaking process in the prior art. Comprising the following steps: carrying out biochemical fingerprint analysis on fresh ursodesoxycholic acid and fructus lycii, and carrying out efficacy typing according to the contents of ursodesoxycholic acid, lycium barbarum polysaccharide, betaine and the like; carrying out targeted bio-enzyme pretreatment on the specific Chinese wolfberry fruits according to the typing result; raw materials of specific types are subjected to efficacy coupling combination, stepped dynamic alcoholysis soaking is adopted, and components with different dissolution characteristics are fully extracted through low-alcohol pre-dissolution and gradient alcohol rising steps; and finally, filtering, harmonizing and alcoholizing. According to the scheme, raw material standardization and process precision are combined, so that the stability of product quality is ensured, the transfer rate of functional components is increased, and liver and stomach nourishing or immunity enhancing products with definite efficacy can be directionally prepared.

The utility model discloses a chicken gallbladder paste separating and purifying device, which relates to the technical field of chicken gallbladder paste and comprises a separating and purifying device body, the separating and purifying device body comprises a purifying box, a separating box is fixedly mounted at the top of the purifying box, and a separating hole is formed in the bottom end in the separating box. And discharging openings are fixedly formed in the bottom ends of the two sides of the purification box, a rolling unit is arranged in the separation box, and a filtering unit is arranged in the purification box. According to the chicken gall bladder separating device, the rotating grip is arranged to drive the driving rod to promote the rotating shaft to drive the connecting rod to enable the rolling wheel to rotate in the separating box, chicken gall bladders in the separating box are rolled and crushed through rotation of the rolling wheel, bile in the chicken gall bladders is promoted to flow outwards, and then the working efficiency is improved; the separated bile surges into the filter frame and is further filtered by the filter screen plate, so that the purity of the bile is improved.

The invention belongs to the technical field of health care products, and discloses a preparation method and application of lemon vesicle embedded active probiotics. The invention discloses lemon vesicle embedded active probiotics which comprise probiotics and lemon vesicles coating the surfaces of the probiotics, and the lemon vesicles are extracellular vesicles from lemons. The lemon vesicles are used for performing single-cell embedding on the active probiotics, so that the lemon vesicles can resist degradation of gastric acid and bile, the survival rate and colonization rate of the active probiotics in the intestinal tract through the stomach are increased, the balance of the active probiotics in regulating the intestinal microecology is promoted, various functions of the active probiotics are efficiently realized, and the effects of improving the intestinal tract microecology and improving the intestinal tract microecology are achieved. Meanwhile, the natural active ingredients of the lemon vesicles can synergistically reduce inflammatory factors of intestinal tracts. The lemon vesicles provide a brand new probiotic delivery technology for active probiotics, and a new development strategy is brought to the oral probiotic industry in the future.

The present invention relates to a pharmaceutical composition comprising malalixib or a pharmaceutically acceptable salt thereof as an active ingredient. The invention also relates to the use of said pharmaceutical composition as a solid dose medicament. The invention also relates to the use of the pharmaceutical compositions and solid doses described herein for the treatment of cholestatic pruritus and cholestatic liver disease.

The invention provides a pharmaceutical composition for treating cholestatic liver injury, and the pharmaceutical composition contains an effective amount of Ferulic acid and Wogonin. The application creatively focuses on two key links in the cholestatic liver injury process, namely (1) bile acid excretion and (2) intestinal mucous barrier protection, a synergistic component of Ferulic acid and wogonin is provided, and the optimal ratio of Ferulic acid and wogonin is verified through an experimental system. Experiments prove that the cholestatic liver injury can be effectively improved by combined application of the Ferric acid and the Wogonin which are derived from traditional Chinese medicines; in addition, the effect of the medicine composition is obviously superior to the effect of independently using the Ferric acid or the Wogonin. Wide application prospects are realized.

The application discloses a capsule for assisting in protecting chemical liver injury and a preparation method thereof, and belongs to the technical field of health-care food. The capsule is composed of curcuma longa extract 100g, silybum marianum extract 70g, schisandra chinensis extract 60g, microcrystalline cellulose 109.5g, silicon dioxide 7g and magnesium stearate 3.5g, and 1000 capsules are taken as a unit. The curcuma longa extract contains curcumin, the content of which is greater than or equal to 10% (10:1), the silybum marianum extract contains silybin, the content of which is greater than or equal to 20% (25:1), and the schisandra chinensis extract contains schisandrin, the content of which is greater than or equal to 1.5% (12.5:1). The three kinds of plant extracts are scientifically compounded, and the effects of antioxidation, anti-inflammation, liver cell membrane protection and bile secretion regulation are synergistically exerted. Through systematic screening of the types and dosages of the filling agent, the flow agent and the lubricant, the technical problems of poor flowability of the mixed plant extract powder, large filling difference and easy jamming are solved.

The application belongs to the technical field of biomedicine, and discloses a method for constructing a Channa argus bile stasis model. The application discloses application of rifampicin or a pharmaceutically acceptable salt thereof in construction of a fish liver injury model. The application discloses, for the first time, that the antibiotic rifampicin widely used in aquaculture is used to construct a fish liver injury model. The model is closer to the real scene of the use of a large amount of drugs when bacterial diseases break out in production practice. The injury type of the fish liver injury model is clearly directed to bile stasis, which fills the research blank of the disease model of the fish, and is different from common hepatocyte necrosis or fatty liver models.