173 results about "Bile Juice" patented technology

The invention belongs to but is not limited to the technical field of biomedicine, and discloses application of golden amide alcohol ester to preparation of a medicine for treating cholestasis. The in-vitro cell level verifies that the golden amide alcohol ester has relatively strong activity of activating FXR and CAR. The golden amide alcohol ester can relieve the cholestasis symptom of a cholestasis model mouse. The golden amide alcohol ester can obviously reduce the content of total bile acid and bilirubin in cells, relieve cholestasis and relieve hepatocyte damage. The golden amide alcohol ester can regulate and control the protein level of transporters and metabolic enzymes related to bile acid and bilirubin through double excitation of FXR and CAR, relieve cholestasis and achieve the purposes of benefiting gallbladder and removing jaundice.

The invention discloses a method for extracting bilirubin, cholic acid and chenodeoxycholic acid from chicken bile. The method comprises the steps of pretreatment, chicken bile treatment, hydrolysis reduction, chromatographic separation and the like. The method comprises the following steps: slicing frozen chicken gallbladder, heating, unfreezing and melting to denature protein into solid, then cooling, performing three-phase centrifugal degreasing and deproteinization, and adding three enzymes including biliverdin reductase, coenzyme I and glucose dehydrogenase to reduce free biliverdin and combined biliverdin, so as to obtain the biliverdin-free biliverdin-glucose dehydrogenase-free biliverdin-glucose dehydrogenase-free biliverdin-glucose dehydrogenase. The method comprises the following steps: adding cholic acid and chenodeoxycholic acid into a container, adding gluconic acid hydrolase and bile salt hydrolase to hydrolyze biliverdin and bile acid, adding trypsin to hydrolyze impure protein, performing chromatography by using macroporous silica gel, and separating bilirubin, cholic acid and chenodeoxycholic acid by using different molecular space structures to obtain high-purity bilirubin, cholic acid and chenodeoxycholic acid. The method has the advantages of simple process, few types of used solvents, low toxicity, and facilitation of environmental protection and industrial production.

The invention relates to the technical field of health-care food, and discloses a health-care composition containing microbial beneficial bacteria and a preparation method of the health-care composition. Comprising the following components in parts by weight: 30-40 parts of gelatinized starch, 8-15 parts of plant enzyme powder, 2-10 parts of microcapsule compound beneficial bacteria, 3-5 parts of selenium-enriched yeast powder, 0.5-2 parts of an American ginseng extract, 12-20 parts of fructo-oligosaccharide, 1-2.5 parts of curcumin, 7-9 parts of ovalbumin and 5-8 parts of sodium carboxymethyl cellulose. Microcapsule composite beneficial bacteria are prepared by compounding bifidobacterium longum, lactobacillus acidophilus, lactobacillus rhamnosus and lactococcus lactis, bridging with carboxymethylated beta-glucan and a Fe < 3 + >-polyphenol network structure and embedding with a protein-polysaccharide system, and can stably exist in an environment with strong acid and high-concentration bile salt to gastrointestinal tracts; through the synergistic effect of different nutrient substances, the intestinal microbe balance can be improved, and the immunity can be remarkably improved.

The invention relates to the technical field of health wine preparation, and discloses a multi-effect synergistic enhanced bear gall body-building wine compound preparation process which comprises the following steps: taking sorghum, corn and fructus lycii as fermentation base materials, and carrying out double-strain synergistic fermentation by adopting saccharomyces cerevisiae and lactobacillus plantarum; adding fresh ursodesoxycholic acid during fermentation, carrying out in-situ enzymatic debinding conversion by using a compound enzyme system generated by a fermentation system, and converting the bound bile acid into free ursodesoxycholic acid; after fermentation, distilling to obtain raw wine; a molecularly imprinted polymer prepared by taking a wolfberry polyphenol key monomer as a template molecule is adopted to carry out targeted anchoring refining on raw wine, selective capture and enrichment of wolfberry polyphenol are carried out, and finally aging is carried out to obtain a finished product. According to the method disclosed by the invention, upstream double-strain fermentation in-situ enzymatic conversion and downstream molecular imprinting targeted refining are systematically integrated, so that the technical contradictions that the conversion rate of combined bile acid is low and a large amount of polyphenol functional components are synchronously lost during purification and impurity removal in a traditional process are solved.

The invention belongs to the technical field of disease medicine preparation, and particularly relates to nanocellulose, a preparation method thereof and application of the nanocellulose to reduction of bile acid level. The nano-crystalline cellulose is nano-crystalline cellulose fiber or nano-crystalline cellulose crystal; wherein the nano-crystalline cellulose fibers are in a slender whisker shape, the average length of the nano-crystalline cellulose fibers is 250-400 nm, and the nano-crystalline cellulose fibers are partially gathered and wound; the nanocellulose crystal is in a short rod crystal shape, the surface of the nanocellulose crystal is connected with a sulfuric acid half ester group, and the length of the nanocellulose crystal is 50-200 nm. The nanocellulose provided by the invention can achieve the purpose of treating intrahepatic cholestasis in the gestation period by improving the TBA level and improving the filial generation live birth rate; the traditional Chinese medicine composition has no obvious influence on liver and kidney functions, blood sugar, blood fat and the like, and is high in safety; in addition, the nanocellulose does not have potential target organ poison or accumulative effect, the biological safety performance is improved, and the nanocellulose can be used for a long time and is suitable for special crowds during pregnancy.

To provide cellular uptake.SOLUTION: The invention provides a bile acid or a pharmaceutically acceptable salt thereof for use in a method for treating the human or animal body, the method comprises administering to the human or animal body a therapeutic compound, where: a. the bile acid is chenodeoxycholic acid or deoxycholic acid; b. optionally, the bile acid is employed in conjunction with EDTA; c. in the method, the bile acid or salt thereof and EDTA are used to enable or enhance intracellular uptake of the therapeutic compound.SELECTED DRAWING: None

The invention provides rhizoma alismatis purified polysaccharide as well as a preparation method, an identification method and application thereof, the rhizoma alismatis purified polysaccharide is a functional component with a novel structure in a traditional Chinese medicine rhizoma alismatis water extract obtained by further extraction and purification, the administration efficiency is effectively improved, and the pharmacodynamic substances are clarified; in-vivo and in-vitro experiments show that the polysaccharide can improve the antioxidant capacity of the liver and relieve inflammatory response; synthesis and metabolism of liver bile acid can be adjusted, cholestasis of the liver is relieved, and bile acid circulation is promoted so as to relieve acute liver injury caused by acetaminophen; the effect of promoting the recovery of liver injury patients is obvious; and the compound also can be used as prebiotics to improve the homeostasis of intestinal microorganisms and promote the synthesis of short-chain fatty acids so as to have beneficial effects on the liver.

This invention relates to a traditional Chinese medicine combination for treating intrahepatic cholestasis, which is made from the following raw materials in parts by weight: Artemisia capillaris and Glycyrrhiza uralensis 5:1 and 3:1. This invention also provides the preparation of the above-mentioned medicine and its use in treating intrahepatic cholestasis. Its advantages are: the traditional Chinese medicine composition of this invention (Artemisia capillaris and Glycyrrhiza uralensis decoction) has a significant ameliorative effect on intrahepatic cholestasis (non-obstructive type) induced by a 1% CA-containing diet in mice. Artemisia capillaris and Glycyrrhiza uralensis decoction can alleviate weight loss in mice and improve liver necrosis; significantly reduce serum ALT and ALP levels in mice; reduce the content of total bile acids in the liver and decrease the proportion of toxic bile acid taurine deoxycholic acid (TDCA) in the bile acid pool; upregulate liver BSEP protein expression to promote bile acid efflux; and downregulate liver NLRP3 protein expression to alleviate liver necrosis.

The invention provides a liver protection composition as well as a preparation method, functional food and application thereof, and relates to the technical field of food processing. The liver protection composition comprises the following raw materials: dandelion concentrated solution, red ginseng extract and hovenia dulcis thunb. The liver-protecting composition prepared by the invention is remarkable in liver-protecting effect and high in comprehensiveness, can realize the liver-protecting effect from multiple aspects of oxidation resistance, inflammation resistance, biliary secretion promotion and the like, is high in bioavailability, completely selects natural plants as raw materials, and does not contain artificial additives, preservatives or sweeteners.

This invention relates to the use of a recombinant protein ANG in the preparation of medicaments for treating cholestatic liver disease. Specifically, this invention provides the use of the angiopoietin ANG gene, or its protein, or its promoter, for the preparation of medicaments, compositions, or formulations for the prevention, improvement, and / or treatment of cholestatic liver disease. It is particularly effective in treating primary biliary cholangitis and primary sclerosing cholangitis.

The invention discloses a probiotic with the characteristic of high yield of bile salt hydrolase and application of the probiotic in a preparation for preventing or improving cholestatic jaundice, and is characterized in that the probiotic is at least one of a bifidobacterium longum subsp. Infantis QS033 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35879 and a lactobacillus delbrueckii subsp. Lactis QS021 strain with the preservation number of CGMCC No.35880; according to the present invention, the Chukrasone A has the application of the Chukrasone A in the preparation of the product for preventing, relieving or regulating the cholestatic jaundice, and has the advantages of effective degradation of sodium glycocholate, sodium glycodeoxycholate, sodium glycochenodeoxycholate and sodium glycoursodeoxycholate, effective regulation of bile acid metabolism disorder in the cholestatic jaundice, and improvement of the cholestatic jaundice.

The invention provides a high-fidelity calculus bovis and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. According to the technical scheme provided by the invention, the high-fidelity calculus bovis is accurately prepared by taking the mean value of the contents of various components of the natural calculus bovis as a reference and accurately measuring the contents of various components in the raw materials, fully utilizing the original components of the ox bile or ox gall powder, not adding other non-calculus bovis components and combining the existence forms of the components. The problems that existing artificial calculus bovis is greatly different from natural calculus bovis in efficacy, and toxic and side effects are relatively high are solved. The shape, the forming process and the components of the product are highly similar to those of natural calculus bovis; the medicine effect of the calculus bovis is improved, and the production cost is reduced.

The invention relates to the technical field of biological pharmacy, in particular to a method for extracting bile acid from pig bile, which comprises the following steps: (1) taking the pig bile, adjusting the pH value of the pig bile to be alkaline, heating the pig bile to 80-90 DEG C, stirring at constant temperature for 25-35 minutes, then cooling to room temperature, and adjusting the pH value of the system to be neutral to obtain pretreated pig bile; (2) filtering the pretreated pig bile in the step (1) by using a hyperbranched polymer film, concentrating filtrate under reduced pressure, adding an ethanol solution, heating to reflux, stirring for 25-35 minutes, and then filtering to obtain a crude bile acid product; and (3) adding a hydrochloric acid solution into the crude bile acid product in the step (2), adjusting the pH value to 2-3, centrifuging, filtering, washing and drying the obtained solid, adding ethyl acetate, heating to reflux until the solid is dissolved, cooling to room temperature, stirring for 2-4 hours, recrystallizing, separating and drying to obtain the bile acid. The extraction method is simple, and the extraction purity is high.

The present invention relates to the technical field of fowl bile processing, and in particular to a method for detecting the bile acid content in fowl bile based on turbidity, which comprises the following steps: adjusting a solution containing bound bile acid to alkaline to obtain a suspension; and then measuring the OD value of the suspension. 600 This solution can solve the technical problems of long time and high cost in detecting bile acid content in poultry bile. When the purchased poultry bile raw materials arrive at the inspection site, this method can be used to conduct a preliminary test on the bile acid content in the poultry bile, and complete the on-site inspection work. Unqualified products with too low bile acid content can be returned on the spot, which greatly saves time and cost. This solution can be applied to practical operations such as the quality inspection of poultry bile, the research and development and production of poultry bile powder and artificial bear bile powder.

The application relates to the embedding technical field, and particularly discloses a double emulsion gel bead embedding beta-carotene and probiotics and a preparation method thereof. The double emulsion gel bead comprises a composite layer, an oil phase and an inner water phase from outside to inside. The composite layer comprises an outer water phase and calcium alginate which are fused with each other. The outer water phase comprises a product after carrageenan and sodium caseinate are subjected to a Maillard reaction. The oil phase comprises beta-carotene, vegetable oil and a food emulsifier. The inner water phase comprises probiotics. In the gel bead, the beta-carotene and the probiotics are simultaneously embedded in the double emulsion gel bead, the calcium alginate hydrogel shell starts to decompose when entering the intestinal tract, then the double-layer emulsion is slowly decomposed after being subjected to chemical digestion of pancreatic juice, bile and small intestinal juice and mechanical digestion of the small intestine in the small intestine, and the beta-carotene and the probiotics are gradually released, the beta-carotene is converted into vitamin A in the intestinal tract and is absorbed, and the probiotics are delivered to the colon to play a probiotic effect.

The present application provides a compound of formula (I), or an optical isomer thereof, a pharmaceutically acceptable salt, a solvated form, or a prodrug. The compound may modulate NTCP function for the prevention and / or treatment of HBV / HDV and hepatitis virus-related diseases, bile acid-related cholestasis disorders, metabolic disorders, and / or liver diseases. Additionally, the present application relates to a pharmaceutical composition comprising such a compound and a method for modulating NTCP.

The bear bile sterilization and disinfection device comprises a bottom plate and a sterilization box fixedly installed on the upper surface of the bottom plate, the top of the inner wall of the sterilization box is fixedly connected with an electric heating plate and a sterilization pot respectively, and a cover plate is hinged to the rear side of the upper surface of the sterilization box. A servo motor is fixedly mounted in the center of the upper surface of the cover plate, and an output shaft of the servo motor penetrates and extends into the sterilization pot and is provided with a stirring assembly. According to the device disclosed by the utility model, the servo motor, the stirring assembly, the ultraviolet lamp and the quartz glass protective cover are arranged, so that bear bile can be synchronously treated in two modes of ultraviolet sterilization and high-temperature heating sterilization, the bear bile is more thoroughly sterilized and disinfected, and the servo motor can run to drive the stirring shaft and the stirring blades to stir the bear bile; and moreover, the bear bile is heated more uniformly and quickly through stirring, so that the sterilization and disinfection speed of the whole device on the bear bile is improved, and the working efficiency is relatively high.

The present invention relates to a molecular association of a bile acid or a bile salt and a composition containing the same, and more specifically, to a skin-permeable formulation of deoxycholic acid, a lipolytic pharmacological agent, which has been developed as an external preparation rather than a conventional injection, and which dramatically improves patient compliance.

The utility model discloses a bilirubin extraction pretreatment device based on membrane separation, which comprises a filter tank used for filtering impurities in pig bile; the multiple feeding ports are formed in the top of the filter box and used for adding pig bile and medicaments into the filter box; the stirring box is communicated with the filtering box and is used for carrying out stirring pretreatment on the filtered pig bile; when the brush moves back and forth on the filter plate, impurities in pig bile are swept to waste plates at the two ends of the filter plate, waste is collected by placing a collecting barrel at the bottoms of the waste plates, the situation that the surface of the filter plate is blocked and accumulated is avoided, the effect of removing the impurities on the surface of the filter plate without shutdown is achieved, the continuity of the bilirubin preparation process is improved, and the production efficiency is improved. The preparation efficiency is improved, the product quality of the bilirubin in the subsequent enzyme catalysis synthesis-membrane separation coupling purification process is improved, the equipment does not need to be shaken in the operation process, and the stability is good.

The present invention belongs to the field of microorganisms, and specifically relates to a plant lactobacillus and an application thereof. The plant lactobacillus is deposited in the Guangdong Microbial Culture Collection Center, and the deposit number is GDMCC No: 63911. The plant lactobacillus is used to ferment cattle bile, and a large amount of bile salt hydrolase is produced during the fermentation process, which can convert the bound bile acid in the cattle bile into free bile acid, and a high conversion rate can be achieved within 2-3 days, and the utilization value is high.

The present application belongs to the technical field of bear gall powder production, and provides a bear gall powder production method and a freeze-drying device. The method comprises the following steps: taking bear bile and filtering, obtaining a filtered bile solution after filtering; placing the filtered bile solution in a condition of-40 DEG C to-45 DEG C for pre-freezing treatment for 1.5 to 2.5 hours, keeping for 1.5 to 2.0 hours after pre-freezing; increasing the temperature to-10 DEG C to-15 DEG C, keeping for 1.5 to 2.5 hours; then controlling the temperature to increase in stages, keeping in stages, increasing the temperature to 37 DEG C to 40 DEG C and keeping, obtaining dry materials; sieving the dry materials, obtaining bear gall powder. The method can effectively reduce energy consumption, shorten processing time, and obtain bear gall powder with less impurities, low moisture, high purity and stable quality. The device has more uniform heat conduction, more sensitive temperature regulation, and can better meet the temperature increasing, temperature decreasing, keeping and other regulation and control requirements of the bear gall powder freeze-drying process.

The dehydrator for gall bladder processing comprises a base, a first motor is connected to the left side of the top end of the base through a screw, one end of a first driving rod is installed at the output end of the lower surface of the first motor, and the other end of the first driving rod extends into an inner cavity of the base; the bottom end of the first driving rod is rotationally connected to the inner wall of the base through a bearing, and a first driving wheel is installed on the outer wall of the first driving rod. The device has a self-cleaning function, residual bile and other substances in the box body can be rapidly and thoroughly removed after each time of processing is completed, cross contamination of materials during processing of different batches of gall bladders is effectively avoided, it is ensured that each batch of gall bladders processed products are higher in purity and more stable in quality, manual cleaning is not needed, operation is easy and convenient, and time and labor are saved; the whole processing efficiency is greatly improved, the sanitary standard is met, the use is convenient, the practicability is high, and the use requirements on the existing market are met.

The invention discloses an oxgall-tolerant in-vitro calculus bovis culture transformation strain and application thereof, and belongs to the technical field of calculus bovis culture. The strain is obtained by separating and purifying fresh ox bile, is identified to belong to escherichia coli, has excellent ox bile tolerance, has the characteristics of beta-glucuronidase, high bezoar conversion rate and high safety, and can be used in the production of in-vitro artificial cultivation of bezoar. The strain is a brand new strain for in-vitro calculus bovis transformation, not only enriches a strain resource library of calculus bovis transformation bacteria, but also provides a new thought for deep research of subsequent in-vitro calculus bovis culture.

The utility model provides a hepatopancreatic ampulla metabolism device which comprises a main drainage tube, a branch drainage tube is connected to the main drainage tube, a medicine release mechanism is arranged at the position, close to the inlet end, of the tube wall of the main drainage tube, and protrusions are arranged on the tube wall of the main drainage tube. The main drainage tube and the branch drainage tube are arranged to drain bile and pancreatic juice at the hepatopancreatic ampulla, discharge blocking of the bile and the pancreatic juice caused by abnormal metabolism at the hepatopancreatic ampulla is avoided, the medicine release mechanism is arranged to release medicine to the hepatopancreatic ampulla, inflammation caused by abnormal metabolism at the hepatopancreatic ampulla is solved, and the hepatopancreatic ampulla is prevented from being damaged by abnormal metabolism at the hepatopancreatic ampulla. And by arranging the bulges, bile and pancreatic juice excreted by drainage are uniformly mixed.

The invention discloses a pet cat food formula and a making process thereof. The pet cat food formula comprises animal basic components: 25% of enzymolysis fresh chicken with bones, 30% of fresh chicken, 10% of fresh complete quail, 8% of fish scale-containing fresh salmon, 5% of fresh chicken liver, 4% of fresh chicken heart and 3% of fermented animal oil; the functional additives comprise 6% of functional fruit and vegetable groups, 2% of freeze-dried queen bee larva powder, 0.5% of propolis, 0.5% of wall-broken bee pollen, 2% of euphausia superba powder, 1% of ostrich liver powder, 1% of catgrass powder and 0.5% of eggshell membrane powder; nutritional additives: 2% of microencapsulated egg yolk powder and 0.2% of a probiotic post-spraying bag; and 0.3% of montmorillonite. According to the method, the meat with bones is pretreated through an enzymolysis technology, macromolecular protein and calcium are pre-decomposed, and the protein digestibility and the calcium bioavailability are remarkably improved; the fermented animal oil is decomposed into smaller molecules and can be directly absorbed without bile emulsification, so that the fat absorption rate is higher; the microencapsulation technology protects the activity of thermosensitive nutrients, and the overall nutrition utilization efficiency is greatly improved.

Provided herein are formulations for use in enteral tube feeding, that contain one or more whole food-based ingredients, mineral nutrients, small molecules, and / or large molecules, the combination of which is effective to bind bile acids when administered to a subject in need thereof. The combination of crafted ingredients for tube feeding reduce symptoms of gastroesophageal reflux, vomiting, abdominal pain, irregular stooling (diarrhea or constipation) and to reduce organ damage including pneumonia, pharyngitis, aspiration, esophagitis, gastritis and enteritis.

This invention relates to the field of feed additive technology, specifically to a feed additive that promotes digestion and absorption and its preparation process. The preparation process of a feed additive that promotes digestion and absorption includes: preparing antioxidant-modified chicken fat; preparing an extract; preparing a composite oil phase; and preparing the feed additive. In this invention, the extract of Shenqu (a type of fermented wheat) can directly compensate for insufficient endogenous enzyme secretion in laying hens, rapidly decomposing starch in feed and chicken fat in plant proteins, laying the foundation for subsequent absorption. The extract of Zhishi (a type of immature bitter orange) accelerates gastrointestinal motility and digesta emptying, while also promoting bile and pancreatic juice secretion, further improving the digestibility and utilization rate of fat-soluble components in laying hens. The extract of pine pollen can repair the intestinal mucosa, increase intestinal villus height, increase the nutrient absorption area, and regulate the intestinal microecology, reducing nutrient loss and intestinal stress. The combined use of these three ingredients synergistically promotes the digestive and absorptive capacity of laying hens.

This invention discloses a method for fabricating a memristor based on a bile functional layer, belonging to the field of bioelectronic device fabrication and in vitro diagnostics of biliary tract diseases. The method sequentially completes FTO substrate pretreatment, bile functional layer preparation, Ag-on-electrode deposition, and memristor detection array fabrication. First, the FTO substrate is physically cleaned and plasma-activated. Then, a 240-260 nm bile functional layer is prepared. Subsequently, an Ag-on-electrode is deposited, and a 5-15 nm Ag atom embedding transition region is constructed. Finally, a 2×2 array is arranged and passivated for isolation. The process parameters of each step are synergistically matched to optimize the interface between the substrate, functional layer, and electrode, fully preserving bile pathological information. The resulting Ag / Bile / FTO structure memristor exhibits high electrical stability and shows significant differences in electrical response to bile samples from normal, gallstone, and gallbladder cancer patients. It enables accurate and rapid in vitro identification of biliary tract diseases, and the process is simple, low-cost, and suitable for clinical application and large-scale production.

The invention discloses an extraction method of 5 alpha-carp cholesteryl sulfate, and belongs to the technical field of extraction and purification of bile acid active substances. The extraction method of 5 alpha-carp cholesteryl sulfate comprises the following steps: (1) carrying out freeze drying or rotary evaporation concentration on fresh fish bile to obtain a crude product; (2) mixing and dissolving the crude product obtained in the step (1) with hot methanol at 50-80 DEG C according to a mass ratio of the crude product to the methanol of 1: (5-10), carrying out suction filtration while the crude product is hot, and collecting filtrate; and (3) ethyl acetate is added into the filtrate obtained in the step (2), the mass ratio of the methyl alcohol to the ethyl acetate is 1: (1-2), standing is conducted, a solid is separated out, and the obtained solid is the 5 alpha-carp cholesteryl sulfate. The extraction method disclosed by the invention is simple and convenient, high in yield, high in product quality and low in cost.

The invention discloses a multi-segment gastric bypass stent, which comprises a support frame main body and a separator arranged outside the support frame main body in a wrapping manner, the support frame main body sequentially comprises a first segment, a second segment, a third segment and a fourth segment from top to bottom, and the first segment is used for being matched, abutted and matched with a gastric antrum part in a limiting manner; the second section is used for abutting against the duodenum ball part in a limiting fit mode. The third section is provided with a framework and is arranged in the duodenum; the diameter of the fourth section is larger than that of the third section, and the fourth section is matched with the peristalsis of the duodenum to stretch the support frame main body towards the extension direction of the support frame main body; the third section comprises a non-clinging section matched with the hepatopancreatic ampulla, and the non-clinging section is used for discharging bile and pancreatic juice from the hepatopancreatic ampulla; and the separator is used for separating the chyme from the duodenum. The multi-section stomach bypass support is reasonable in structure, can prevent the stomach bypass support from blocking the hepatopancreatic ampulla, guarantees normal work of the hepatopancreatic ampulla, and avoids the occurrence of diseases such as pancreatitis.