High efficiency technique for extracting bilirubin and bile acid by using animal bile as raw material

A technology for bilirubin and bile, applied in the field of extracting cholic acid, bilirubin, and deoxycholic acid, which can solve the problems that the product output is difficult to meet the market demand, and there are no high-purity bile acid and bilirubin products.

Inactive Publication Date: 2008-03-26
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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AI Technical Summary

Problems solved by technology

The extraction of bile acid and bilirubin started in the 1960s in foreign countries, and now the technology is mature, the equipment is advanced, the product is of high purity, and the quality is good; my country has started the extraction of b

Method used

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Embodiment Construction

[0005] The raw materials used for the synthesis of the present invention: fresh bile (provided by Tianjin Meat Joint Processing Plant), concentrated hydrochloric acid, chloroform, sodium hydroxide, ethanol (Tianjin Chemical Reagent Company). The melting point was determined with an X-4 melting point apparatus. IR was measured with a Bio-RAD FTS 135 Fourier transform infrared spectrometer, and KBr pellets were used. 1 HNMR was determined by Varian Unity Plus-300 nuclear magnetic resonance instrument, CDCl 3 as solvent and TMS as internal standard. Elemental analysis was determined with a FOSSHeraeus elemental analyzer.

[0006] Implement operation

[0007] 1) Degreasing: Cool the fresh bile until the precipitated solids no longer increase, filter to remove the solids.

[0008] 2) Hydrolysis: Add sodium hydroxide to the deesterified bile, adjust the pH to 9-10, heat to boiling, keep boiling for 10 minutes, and cool.

[0009] 3) Extraction: add a small amount of sodium bisul...

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Abstract

The present invention relates to process of extracting cholic acid, deoxycholic acid and bilirubin from bile of pig, ox and sheep. Bile of pig, ox and sheep consists of water in about 97 %, bile acid in about 2.5 % and bilirubin in about 0.4 %; and contains also phospholipid, cholesterol, Na, K, Ca, phosphate, carbonate, small amount of protein, and other components. Fresh bile is treated through cooling, filtering to defat, basic hydrolysis, acidification and organic solvent extracting to obtain bilirubin; the rest solution is further treated through deep saponification, acidification and organic solvent precipitation to obtain the mixture of cholic acid and deoxycholic acid; and the mixture is re-crystallization separated to obtain high purity cholic acid and deoxycholic acid.

Description

technical field [0001] The invention relates to a method for extracting cholic acid, deoxycholic acid and bilirubin from the bile of pigs, cattle and sheep. It is characterized in that it is used for preparing artificial bezoar and other functional medicines and materials. Background technique [0002] Bile is a special body fluid that flows in the biliary tract and is secreted by the mammalian liver. After being formed in the liver, it enters the gallbladder through the cystic duct and is stored there; it is released from the gallbladder after eating to digest the fat and lipids in the food. The vast majority of bile is water (about 97%), and there are many substances dissolved in water, including bile acids (about 2.5%) that can help fat digestion and absorption, and liver excretion bile that has nothing to do with digestion. Red pigment (about 0.4%). In addition, bile contains phospholipids, cholesterol, sodium, potassium, calcium, phosphate, carbonate, and a very smal...

Claims

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Application Information

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IPC IPC(8): C07J9/00C07D207/44A61K35/413
Inventor 胡祥正刘安军刘玉江
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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