37 results about "Hyocholic acid" patented technology

The invention discloses a chemical synthesis method of intermediate 7-ketolithocholic acid (3alpha-hydroxy-7-one-5beta-cholestan-2-4-acid) of ursodeoxycholic acid, and belongs to the field of organic chemical synthesis. According to the method, cholic acid is adopted as a material, and is subjected to reactions including selective oxidation of 7alpha-hydroxy, benzyl esterification of a side chain carboxyl group, esterification of 3alpha-hydroxy, methanesulfonic acid esterification of 12alpha-hydroxy, elimination, hydrogenation and hydrolysis to synthesize the intermediate 7-ketolithocholic acid of the ursodeoxycholic acid; the cheap cholic acid is adopted as the material, the synthesis method has the advantages of being novel, low in cost, high in yield and environment-friendly and industrial production is facilitated.

The invention discloses a method for extracting cholic acid of ducks and chenodeoxycholic acid (CDCA) from bile of ducks. The method is used for directly extracting CDCA from the bile of ducks and is also used for extracting cholic acid of ducks. The preparation method is simple, is easy to operate and is suitable for industrial application. The preparation method can be used for preparing CDCA and cholic acid of ducks at the same time. The purity of the prepared CDCA and cholic acid of ducks is higher and is more than or equal to 95% and more than or equal to 90% respectively.

The invention discloses a preparation method of alpha-murine cholic acid. The method takes 3alpha, 6alpha, 7alpha-trihydroxy-5beta-cholanic acid (hyocholic acid) as an initial raw material, and comprises the following steps: carboxyl esterification, acetylation of 3-site and 7-site hydroxyl, oxidation of 6-site hydroxyl, reduction of 6-site carbonyl, hydrolysis and recrystallization to obtain high-purity 3alpha, 6beta, 7alpha-trihydroxy-5beta-cholanic acid, namely alpha-murine cholic acid. The alpha-murine cholic acid synthesized and prepared by the route is wide in raw material source; and the method has the advantages of high yield, high purity and few side reactions, and is suitable for large-scale preparation.

The invention discloses a method for extracting ursodesoxycholic acid. The method for extracting ursodesoxycholic acid comprises the steps of esterification, extraction, recrystallization, hydrolysis, secondary recrystallization and the like. According to the method for extracting ursodesoxycholic acid, porcine bile is taken as an extraction raw material, after bilirubin is extracted from the porcine bile, filtrate after hyodeoxycholic acid and hyocholicacid are extracted is collected, and separation is carried out to obtain the ursodesoxycholic acid; porcine bile raw material is available, so that preparation cost of the ursodesoxycholic acid is reduced; acetic anhydride is selected as an esterifying reagent in an extraction process, ursodesoxycholic acid and recrystallization refining conditions are determined, sodium hydroxide is adopted as an alkali catalyst, and total yield of the ursodesoxycholic acid is more than 15% by adopting the technologic process.

The invention provides application of bile acid in the preparation of additives for improving the growth performance of weaned piglets. The bile acid includes the following components: hyocholic acid,hyodeoxycholic acid and chenodeoxycholic acid. The bile acid is proved by experiments that adding the bile acid to daily rations of weaned piglets can improve the growth performance, improve the digestibility of nutrients (especially fat) and reduce the anti-nutritional factors of plant proteins, thereby comprehensively improving the growth performance of piglets, improving the efficiency of feedutilization and the evenness of final weight, reducing the cost of meat production and improving the economic benefits and returns.

The invention discloses a method for extracting chenodeoxycholic acid, and belongs to the technical field of bioengineering. The method comprises the following steps of (1) preparing bile acid sodium salt; (2) preparing bile acid amine salt; (3) extracting and separating a bile acid amine salt solution; (4) salting out and carrying out alkaline hydrolysis. According to the method, when the chenodeoxycholic acid is extracted, chenodeoxycholic acid amine salt and cholic acid amine salt regulated through organic acid in a butyl ester solution have a remarkable solubility difference, so that the chenodeoxycholic acid amine salt and the cholic acid amine salt are crude-separated, and the content of cholic acid is reduced to below 3 percent through crude separation; the chenodeoxycholic acid amine salt and the cholic acid amine salt have a larger solubility difference in a high-concentration butyl ester solvent and a dichloromethane water solution system, and the cholic acid is cleaned and treated again, so that the chenodeoxycholic acid is refined and purified, the content of the cholic acid is reduced to be within 0.2 percent, and the extraction ratio of the chenodeoxycholic acid is greatly improved.

The invention provides a composition applied on a navel for preventing and treating obesity, belonging to the technical field of obesity treatment. The composition is prepared from cattle bitter gallpowder, pig bitter gall powder, radix et rhizoma rhei and ginger juice; the weight ratio of the cattle bitter gall powder to the pig bitter gall powder to the radix et rhizoma rhei is (10-20) to (10-20) to (1-2), the ratio of the mass of the cattle bitter gall powder to the volume of the ginger juice is (10-20)g to (2-5)ml; the cattle bitter gall powder is prepared from taurocholic acid, calculusbovis deoxycholic acid, glycocholic acid and glycodeoxycholic acid hydrate; the pig bitter gall powder is prepared from hyocholic acid and hyodeoxycholic acid. The composition for preventing and treating obesity can be used for reducing weight, and any side effects are not seen.

The invention especially discloses a bile acid composition and a preparation method thereof. The bile acid composition has effective ingredients including hyocholic acid, hyodeoxycholic acid and chenodeoxycholic acid, wherein the sum of the weight percentages of hyocholic acid and hyodeoxycholic acid is not less than 78.0%, the weight percentage of chenodeoxycholic acid is not less than 18.0%, and the balance is water and ash. The bile acid composition and the preparation method thereof have the beneficial effects that: the preparation method has the advantages of simple operation and mild conditions, does not adopt harmful and toxic organic solvents in the preparation process, and can be easily applied in industrialized production; the prepared bile acid is high in yield and high in purity, is added into a feed for feeding broiler chickens, can significantly improve the growth performance and slaughter performance of the broiler chickens, is beneficial for the broiler chickens to utilize fat, reduces the use of broiler chicken own fat, thereby improving the growth performance and slaughter performance of the broiler chickens, solving the problems of fat digestion and absorption in the feed, and improving the broiler chicken carcass quality.

The invention discloses a method for removing hyocholic acid from chenodeoxycholic acid. The method comprises the steps of dissolving, residue removing, complexing, crystallizing, centrifugal separating, emulsifying, acidifying, drying, mother liquor treating and the like; high-boiling-point butyl acetate is utilized as solvent, volatilization of the solvent is reduced, a good separation effect is achieved by utilizing the unique dissolution property of butyl acetate to impurities, and the solvent belongs to a three-level low-toxicity dangerous chemical; the selective complexing characteristic of sec-butylamine to bile acid is utilized, and the hyocholic acid and other impurities can be separated to a higher degree. According to the separation and purification method, impurity removing is qualified in one time, the loss rate of the chenodeoxycholic acid is 5% or below and is greatly lower than the effective ingredient loss of 30% in a conventional crystallization method, the solvent can be recycled, three wastes are not generated, and the method is an environment-friendly, low-cost and efficient separation method.

The invention provides a method for preparing chenodeoxycholic acid and ursodesoxycholic acid by directly extracting and synthetizing from porcine bile paste or leftovers, comprising the following steps: using lower content (15-35%) porcine bile paste or leftovers and pre-processing it to remove off non-cholic acids impurities; removing off cholic acid impurities below the chenodeoxycholic acid in the thin layer chromatography by bromine oxidation or chromium oxidation; removing off cholic acid impurities above the chenodeoxycholic acid or the ursodesoxycholic acid in the thin layer chromatography by conventional reduction method; directly preparing the chenodeoxycholic acid or the ursodesoxycholic acid.

The invention discloses a 7-ketolithocholic acid intermediate and its preparation method and application. The invention uses hyocholic acid as a starting material, first performs esterification reaction, and then adds 2,2-dimethylpropane and hyocholic acid The unique 6,7 hydroxyl structure reacts to form parallel fork protection, and then selectively protects the 3-position hydroxyl group, and then uses the special space structure of hyocholic acid to selectively connect the leaving group to the 6-position to form 7-ketone Keystone cholic acid intermediate, 7-ketolithocholic acid intermediate unique 7-position exposed hydroxyl group, selective oxidation of 7-position hydroxyl group to form α-hydroxy ketone intermediate 6 with 6-position connecting leaving group, this type of hydroxyketone can be directly The hydroxyl group at the 6th position and the leaving group are removed simultaneously to form intermediate 7, and intermediate 7 can be deprotected by simple hydrolysis to obtain the target product 7-ketolithocholic acid. The 7-ketolithocholic acid prepared by the method of the present invention not only has high purity, but also has a simple process flow and no special purification method in the preparation process, which is suitable for industrial production.

The invention discloses a 7-ketolithocholic acid intermediate and its preparation process and application. The invention uses hyocholic acid as a starting material, and first performs an esterification reaction, and then a silane-type protecting group protects the 3-position with high selectivity. Hydroxyl, and then using the specific spatial structure of the 6 and 7 hydroxyl groups of hyocholic acid, the conventional protection method can selectively connect a strong leaving group at the 6-position to obtain the intermediate of 7-ketolithocholic acid, 7-ketolithocholic acid The acid intermediate is sequentially oxidized, removed, reduced, and hydrolyzed to remove the protecting group to obtain the target product 7-ketolithocholic acid. The 7-ketolithocholic acid prepared by the process of the present invention not only has high purity, but also has a simple process flow, greatly simplifies the synthesis route and saves industrialization costs.

Disclosed is an identification method for fermented and mixed-steamed bile arisaema processed from porcine bile. The identification method includes: taking hyocholic acid (HCA), hyodeoxycholic acid (HDCA) and chenodeoxycholic acid (CDCA) as a group of markers, taking glycohyodeoxycholic acid (GHDCA) and glycochenodeoxycholic acid (GCDCA) as another group of markers, and applying the groups of markers in identifications of the fermented and mixed-steamed bile arisaema processed from the porcine bile. According to the identification method, thin-layer chromatography and high-performance liquid chromatography are provided to identify bile acid ingredients in the bile arisaema processed from the porcine bile, so that different products of the bile arisaema processed from the porcine bile can be identified simply and quickly, and quality of the bile arisaema can be controlled better. The identification method has the advantages of high specificity and good reproducibility.

The invention discloses a method for extracting chenodeoxycholic acid and allocholic acid from duck bile, and belongs to the technical field of biological engineering. The method comprises the following steps of (1) preparing of bile acid extracting liquid: obtaining duck bile, saponifying, cooling, adjusting a pH (potential of hydrogen) value to 7 to 8, adding a mixed solvent of ester and alkane, adjusting the pH value to 2 to 4, extracting, and discoloring, so as to obtain the bile acid extracting liquid; (2) preparing of bile acid magnesium salt: adjusting the pH value of the bile acid extracting liquid in step (1) to 4.5 to 5.5, dissolving the magnesium salt, adding into the bile acid extracting liquid, refluxing, cooling, and separating, so as to respectively obtain the magnesium allocholate and a chenodeoxycholic acid solution; refining and purifying the chenodeoxycholic acid solution, so as to obtain a finished product of henodeoxycholic acid; (3) extracting of the allocholic acid: adding water and carbonate into the prepared magnesium allocholate in step (2), heating and dissolving, separating solid from liquid, adjusting the pH value of a solution to 1 to 2, and separating solid from liquid, so as to obtain a finished product of the allocholic acid. The method has the advantages that the purities and extracting rates of the chenodeoxycholic acid and the allocholic acid are greatly improved, the operation is safe, and the method is suitable for industrialization.

According to the method, chenodeoxycholic acid, hyocholic acid and hyodeoxycholic acid do not need to be separated and purified, but total bile acid in the pig gall subjected to bilirubin extraction is directly catalyzed by a compound enzyme, and then the ursodeoxycholic acid is synthesized by the ursodeoxycholic acid and the pig gall subjected to bilirubin extraction. Compared with the prior art, the method has the advantages that the conversion yield of the ursodesoxycholic acid can be increased by more than one time, meanwhile, the problem that three bile acids and reaction intermediate products thereof are impurities in the prior art is solved, the problem that purification needs to be performed firstly in the traditional conversion method is solved, the use of organic solvents in the reaction process is reduced, and the production cost is reduced. And after the reaction is finished, recycling can be realized, and environmental pollution is reduced.