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Method for extracting cholic acid of ducks and chenodeoxycholic acid (CDCA) from bile of ducks

A technique for chenodeoxycholic acid and duck cholic acid, which is applied in the field of preparation of chenodeoxycholic acid, can solve the problems of slow precipitation, complicated process and low efficiency, and achieves the effects of easy operation and simple preparation method.

Inactive Publication Date: 2016-04-13
四川新功生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, the traditional process of extracting chenodeoxycholic acid from the bile of chickens, ducks and geese includes steps such as saponification of bile, acidification, removal of alcohol insolubles, fat removal, formation of barium salts, and removal of barium. The process is complicated. Low yield, difficult for industrial application
Or use the calcium salt precipitation method, the precipitation speed is slow, the efficiency is low, and the decolorization effect of hydrogen peroxide and activated carbon is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for extracting duck cholic acid and chenodeoxycholic acid from duck bile, comprising the following steps:

[0032] A, Extraction of Crude Bile Acids

[0033] Take 1000g of fresh duck bile and put it into the reaction kettle, add 0.35 times the amount of sodium hydroxide, continue to put in, seal and keep the pressure, heat up to 120°C, stir for 12 hours, let it stand down to room temperature, add dilute hydrochloric acid to adjust the pH value to 3 to get a black paste Material 200g. Add 3 times the amount of methanol to the black paste, heat to 50°C, stir until completely dissolved; continue to add 3 times the amount of petroleum ether and stir for 30 minutes, let it stand, siphon to remove the petroleum ether layer; continue to add 3 times the amount of petroleum ether , and repeat 2 more times; concentrate the petroleum ether layer to recover petroleum ether, and the concentrate is grease, which is discarded. The methanol layer was concentrated in vacuo be...

Embodiment 2

[0039] A method for extracting duck cholic acid and chenodeoxycholic acid from duck bile, comprising the following steps:

[0040] A, Extraction of Crude Bile Acids

[0041] Take fresh duck bile and put it into the reaction kettle, continue adding 0.3 times the amount of sodium hydroxide, seal and keep the pressure and raise the temperature to 125°C, stir and react for 12 hours, let it stand at room temperature, add dilute hydrochloric acid to adjust the pH value to 2.8 to get a black paste . Add 4 times the amount of methanol to the black paste, heat to 50°C, stir until completely dissolved; continue to add 3 times the amount of petroleum ether and stir for 30 minutes, let it stand, siphon to remove the petroleum ether layer; continue to add 3 times the amount of petroleum ether , and repeat 3 times; concentrate the petroleum ether layer to recover petroleum ether, and the concentrate is grease, which is discarded. The methanol layer was concentrated in vacuo below 70°C to ...

Embodiment 3

[0047] A method for extracting duck cholic acid and chenodeoxycholic acid from duck bile, comprising the following steps:

[0048] A, Extraction of Crude Bile Acids

[0049] Take fresh duck bile and put it into the reaction kettle, continue adding 0.4 times the amount of sodium hydroxide, seal and keep the pressure and raise the temperature to 115°C, stir and react for 13 hours, let it stand to cool down to room temperature, add dilute hydrochloric acid to adjust the pH to 2.8 to get a black paste . Add 3 times the amount of methanol to the black paste, heat to 50°C, stir until completely dissolved; continue to add 3 times the amount of petroleum ether and stir for 30 minutes, let it stand, siphon to remove the petroleum ether layer; continue to add 3 times the amount of petroleum ether , and repeat 2 more times; concentrate the petroleum ether layer to recover petroleum ether, and the concentrate is grease, which is discarded. The methanol layer was concentrated in vacuo be...

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PUM

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Abstract

The invention discloses a method for extracting cholic acid of ducks and chenodeoxycholic acid (CDCA) from bile of ducks. The method is used for directly extracting CDCA from the bile of ducks and is also used for extracting cholic acid of ducks. The preparation method is simple, is easy to operate and is suitable for industrial application. The preparation method can be used for preparing CDCA and cholic acid of ducks at the same time. The purity of the prepared CDCA and cholic acid of ducks is higher and is more than or equal to 95% and more than or equal to 90% respectively.

Description

technical field [0001] The invention relates to a preparation method of chenodeoxycholic acid, in particular to a method for extracting duck cholic acid and chenodeoxycholic acid from duck bile. Background technique [0002] Chenodeoxycholic acid (Chenodeoxycholic acid, referred to as CDCA), is a natural bile acid, which was first extracted from goose bile in 1848. Chenodeoxycholic acid has the ability to dissolve gallstones and other pharmaceutical functions. It is a natural medicine for the treatment of gallstones, and it is also a raw material for the synthesis of ursodeoxycholic acid and other steroidal compounds. [0003] Since chenodeoxycholic acid is the main component of poultry bile such as chicken, duck and goose, it can be directly extracted from the bile of poultry. However, limited by technology, chenodeoxycholic acid products currently on the market are basically extracted from chicken bile. The number of geese raised is small, and it is difficult to use its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
CPCC07J9/005
Inventor 刘新宇周虹
Owner 四川新功生物科技集团有限公司
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