209 results about "Ursodeoxycholic acid" patented technology

The invention discloses a 7beta-hydroxyl sterol dehydrogenase mutant which is obtained by protein engineering and of which coenzyme preference is changed, a coding gene of the 7beta-hydroxyl sterol dehydrogenase mutant, a recombinant expression vector and a recombinant expression transformant which contain a sequence of the gene, a preparation method of a recombinant mutant enzyme preparation, andapplication of the recombinant mutant enzyme preparation to preparation of ursodeoxycholic acid. By co-enzyme regeneration of enzymic coupling, the recombinant mutant enzyme preparation disclosed bythe invention can efficiently utilize relatively cheap oxidized coenzyme I (NAD+) instead of very expensive oxidized coenzyme II (NADP+); asymmetric reduction of catalytic 7-hydroxyl lithocholic acideffectively reduces production cost; moreover, the recombinant mutant enzyme preparation has the advantages of simplicity for operation, mild reaction condition, environmental-friendliness, high yieldand the like, and has a good application prospect in preparation of ursodeoxycholic acid by epimerization of chenodesoxycholic acid.

The invention provides a process of separating anthrodsoxycholic acid by using fresh swine bile as raw material, the invention further provides a process for preparing ursodeoxycholic acid by using fresh swine bile as raw material, which comprises extracting conjugated bile acid from swine bile after acidification, removing foreign materials such as aliphatic acid and protein and the like, preparing purified pig bile acid through hydrolysis (saponification) and hydroperoxide decolourization, extracting anthrodsoxycholic acid through solution immersion cleaning, preparing ursodeoxycholic acid through oxidization and reduction of anthrodsoxycholic acid which is obtained through separating. The process of the invention improves the preparation technology of ursodeoxycholic acid, and achieves positive effects on the aspects of technology simplification and output increase.

The invention discloses a method for synthesizing ursodeoxycholic acid (UDCA) and high-chiral-purity D-amino acid based on an enzyme-method coupling technology. The method comprises the following steps: putting chenodeoxycholic acid and alpha-ketonic acid into a solution system containing 7alpha-HSDH (Homoserine Dehydrogenase), DAADH and NADP (Nicotinamide Adenine Dinucleotide Phosphate) and carrying out enzyme catalysis reaction; separating a reaction solution by adopting an ultra-filtration membrane to obtain a concentrated mixed enzyme solution; regulating the pH (Potential of Hydrogen) ofa dialysis solution and crystallizing; filtering and separating to obtain 7-KLCA wet powder and filtrate; carrying out chromatographic treatment on the filtrate to obtain the D-amino acid; putting the7-KLCA wet powder into a solution system containing glucose, the NADP, the 7alpha-HSDH and GDH (Glutamate Dehydrogenase) and carrying out enzyme catalysis reaction; separating the reaction solution by adopting the ultra-filtration membrane to obtain the concentrated mixed enzyme solution; crystallizing, filtering and separating the dialysis solution, so as to obtain ursodeoxycholic acid. By adopting the method provided by the invention, UDCA and the high-chiral-purity D-amino acid can be obtained at the same time, the enzyme utilization rate is high, synthesis steps are simple and the cost isreduced; meanwhile, a metal reduction reagent and an organic solvent do not need to be added in a reaction process and conditions are mild; the method is environmentally friendly and is suitable forindustrial production.

A preparation method for ursodeoxycholic acid regards chenodeoxycholic acid (1) as raw materials; in technological processes, after adopting corresponding equipment and adding excipient according to the proportional variety and dosage, chemical reactions are carried out through the process flow of an oxidation process (A), a hydrogenation process (B), and a purification process (C) in particular temperature and environmental conditions. In the oxidation process (A), an oxidant (3) and a solvent I 2 are added for oxidation reaction to generate an intermediate (4); in the hydrogenation process (B), a catalyst (6) and a solvent II(5) are added for hydrogenation reaction to get ursodeoxycholic acid crude products (9); in the purification process (C), a passivating agent (11) and a solvent III (10) are added for producing the products of ursodeoxycholic acid (12). The process route of the invention is reasonable; the chemical reactions are mild and safe; the operating conditions are easy to be controlled; the yield is high and the invention makes great progress in the research and production of artificially synthesizing ursodeoxycholic acid (12).