230results about How to "Easy to separate and extract" patented technology

The invention discloses a comprehensive utilization process of wood cellulose, which comprises the processes of comprehensive treatment, oligosaccharide preparation, lignin preparation, fiber ethanol preparation and the like. The invention has the advantages of decomposing a biomass macromolecular natural compact structure by using a high-pressure instant steam exploding technology, being beneficial to the separation and the extraction of the lignin, the cellulose and the cellulose, reducing the consumption of the energy sources and the materials, such as acid, alkali, heat, catalyst and the like for preprocessing, reducing the emission of pollutants and lightening the pollution of the environment. Secondly, the cogeneration of oligosaccharide and lignin realizes the comprehensive utilization of the energy sources, especially the application of the steam exploding technology ensures that the quality of the two products of the oligosaccharide and the lignin is greatly improved and the yield is greatly increased; and alcohol and the lignin of production fuels of sugar residues can be extracted irrespective of the order.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g/l.

The invention discloses an aero-engine intershaft bearing early weak fault diagnosis method, which belongs to the technical field of aero-engine fault detection. The method includes the following steps: the fault signal of the intershaft bearing is collected; an AR model is used to remove a deterministic signal generated by periodic rotation of the engine rotor; the processed fault signal is subjected to inverse MED filtering to enhance the shock component of the fault signal; a CEEMD method is used to decompose the processed fault signal, an effective modal component is screened according toa kurtosis value index and an autocorrelation function, and the intershaft bearing fault signal is subjected to noise reduction and reconstruction; and through a Teager energy operator, the output energy of the noise reduction signal is obtained, the envelope spectrum is calculated, and through analyzing the frequency component with a frequency component in the envelope spectrum, engine intershaftbearing early weak fault diagnosis is realized. The fault signal can be subjected to preprocessing, screening, reconstruction and demodulation, a fault characteristic signal reflecting the bearing fault is acquired, and the early weak fault of the intershaft bearing can be accurately diagnosed.

A process for extracting lycopene oil resin from tomato or water melon by supercritical fluid (CO2, propane, ethane, or ethene) includes pre-treating for removing sugar, gum and water from raw material, extracting with said supercritical fluid to obtain lycopene oil resin, packing it under protection of inertial gas in dark position or vacuum packing, and low-temp storage. Its advantages are simple process, high efficiency, low energy consumption, no pollution and low cost.

Provided are an extremely high optical voidness D-lactic acid and L-lactic acid fermentation producing strain, a construction method thereof and a method of manufacturing extremely high optical voidness D-lactic, wherein the preservation number of a D-lactic fermentation producing strain is CGMCC No.11059, and the preservation number of an L-lactic acid fermentation producing strain is CGMCC No.11060.

The invention discloses a 7beta-hydroxysterol dehydrogenase mutant with increased activity and stability which is obtained through molecular evolution, recombinant expression plasmid containing the 7beta-hydroxysterol dehydrogenase mutant gene and a recombinant expression transformant and a preparation method of a recombinant mutant enzyme preparation, and the invention also provides an application of the recombinant mutant enzyme preparation in ursodeoxycholic acid synthesis. The 7beta-hydroxysterol dehydrogenase has excellent activity and heat stability, can efficiently catalyze asymmetric reduction of 7-carbonyl lithocholic acid to prepare the ursodeoxycholic acid; the 7beta-hydroxysterol dehydrogenase is subjected to immobilization and then is subjected to couple by an enzyme method with the immobilized 7beta-hydroxysterol dehydrogenase, epimerization of a substrate chenodeoxycholic acid with low cost can be directly catalyzed, ursodeoxycholic acid can be prepared through continuous conversion, and the operation is simple. Compared with the prior art reported currently, ursodeoxycholic acid prepared by hydroxysterol dehydrogenase through catalysis has the advantages of high substrate concentration, short reaction time, complete reaction, and high product purity, and has strong industrial application prospect.

The invention discloses a total alkaloid extract and making method of eye drip, which comprises the following parts: opium alkaline, tetrahydropalmatine, cumic acid, alcaine palmatine, corydaline, summer alkaline and other biological alkalines. The preparing method comprises the following steps: adopting summer non as raw material; optimizing hypercritical CO2 flow to extract; collecting extract; drying; obtaining the total alkalilne extract; fitting for tablet, pill, suppository, percutaneous absorption agent, oral rapid-breaking agent, release-control agent and freeze dried.

The invention discloses a method for producing phosphocreatine by converting creatine and triphosadenine by a microbial enzyme method. According to the method, a genetic engineering method is adopted; recombinant escherichia coli BL21(DE3)-pET21a(+)-CPK which expresses creatine kinase efficiently is constructed; and the reaction of the creatine and the triphosadenine is catalyzed to produce the phosphocreatine by taking fermentation bacteria or lysate as an enzyme source. The invention provides a new green production process method for the phosphocreatine, which has the advantages of mild conditions, short period, few impurities in an enzymatic system, convenience in separation and extraction of products, simple in process steps, safety in operation and the like; and the method has a very good industrial application prospect.

The invention discloses a genetic engineering strain for producing phenazine-1-carboxylic acid, which is produced by taking pseudomonas chlororaphis as a culture, as well as application of the genetic engineering strain; the genetic engineering strain is obtained by knocking out a phzH gene in pseudomonas chlororaphis genome. The genetic engineering strain for producing phenazine-1-carboxylic acid is finally prepared by deleting the phzH gene in the genome of the strain by virtue of insertion mutation or marker-less deletion from pseudomonas chlororaphis HT66 CCTCC NO: M2013467 which can naturally secrete phenazine-1-formamide as well as derivatives of the pseudomonas chlororaphis so as to convert a secondary metabolite from the phenazine-1-formamide into the phenazine-1-carboxylic acid. The invention also discloses a preparation method and a detection method of the fungicide.

The invention discloses a method for preparing levodopa by virtue of an enzymic method. The method comprises the following steps: picking up an annular stenotrophomonas maltophilia strain slant for inoculation into a seed culture medium for culturing, thereby obtaining a primary seed liquid; inoculating a fermentation culture medium with the primary seed liquid at the inoculum size of 3-10% for fermentation culturing, centrifuging after the fermentation is ended and collecting bacterial cells; and adding tyrosine and the bacterial cells to a buffer solution to have an enzymatic reaction under the conditions of 18-30 DEG C and the pH of 5.0-6.0, thereby converting the tyrosine into the levodopa. According to the method, due to the resting cell transformation technology, and the preferred intermittent weak ventilation technology, adopted in the transformation process, the catalytic efficiency of the enzyme is improved; the concentration of the levodopa is 27g/L and the molar transformation rate of the tyrosine is above 99%; and besides, the method is mild in conditions, high in enantio-selectivity and free from racemization effect, is more suitable for industrial production, and has remarkable economic benefit and industrial application value.

The invention discloses a corynebacterium glutamicum mutant strain and application thereof in L-leucine production. The corynebacterium glutamicum mutant strain has a collection number of CGMCC No. 15720. By utilizing the mutant strain disclosed by the invention and a fermentation method, high-efficiency production of the L-leucine can be realized, and the yield of by-product acids can be effectively reduced in the fermentation production process. Furthermore, since the amount of heteroacids in fermentation liquor is small, a seed crystal, a crystal washing agent or a crystallization additivedoes not need to be added according to the conventional scheme in the field in the purification process, and a recrystallization step is not needed. The fermentation liquor is simply separated and extracted, and the product purity is high. The whole production technology is simple and controllable in process, low in cost and suitable for large-scale industrial application.

The invention relates to an array type narrow-band optical filter which comprises a substrate, a first grating and a second grating, wherein the first grating and the second grating are mutually hybridized and nested on the substrate, the first grating and the second grating form a plasmon composite grating, and the period of the plasmon composite grating is the sum of the width of the first grating and the width of the second grating; the first grating sequentially comprises a bottom metal layer, a dielectric layer and a top metal layer from bottom to top along the substrate, and the second grating sequentially comprises a dielectric layer and a top metal layer from bottom to top along the substrate. The invention also provides a preparation method of the array type narrow-band optical filter, and the optical filter prepared by the preparation method has the excellent characteristics of low cost, narrow line width, weak side peak, miniaturization and the like, and has extremely high application prospect and value in the aspects of biochemical sensing, gas flame detection, multispectral imaging and the like.

The invention discloses a method for constructing recombinant yarrowia lipolytica for synthesizing erythritol and a strain of the yarrowia lipolytica. The yarrowia lipolytica is adopted as a syntheticchassis, the yeast is subjected to gene editing through a metabolic engineering improvement means, the capability of erythritol synthesizing is enhanced, and the route of synthesizing by-products isblocked. The yeast strain is yarrowia lipolytica ery949-4 delta CGMCC No. 19351. Glucose with the initial concentration of 50-350 g/L is adopted as a carbon source, and a nitrogen source with the initial concentration of 5-30g/L and inorganic salts are taken as raw materials, high-temperature sterilization and cooling are carried out to be incubated with the yarrowia lipolytica yeast strain, and continuous fermentation or batch supplemented fermentation is carried out under the aerobic conditions; and after fermentation is finished, erythritol is purified from the fermentation broth. The strain can efficiently synthesize erythritol by taking glucose and other carbon source as the raw materials, does not synthesize by-products (such as mannitol and the like), and does not consume erythritol.

The invention relates to a gesture interaction system based on double finger angle domain characteristics and a working method thereof. The gesture interaction system comprises a data acquisition module, a characteristic extraction module, a gesture recognition module and a virtual interaction module. The data acquisition module is used for initializing Leap motion and acquiring gesture information by using the Leap motion; The feature extraction module is used for three-dimensional coordinate preprocessing and gesture feature extraction; The gesture recognition module is used for inputting the extracted double-finger angle domain features into an SVM-KNN classification algorithm to perform gesture classification; And the virtual interaction module is used for connecting Leap motion with Unity 3D to realize different interaction control of different classified gestures on instruments in a gymnasium scene. According to the invention, the user experience is improved, and the user immersion is enhanced.

The invention relates to the technical field of biomedicines, and concretely relates to a microbial fermentation preparation method of bleomycin derivatives. The method is characterized in that SB9026 (with the preservation number of CCTCC M2011292) fermentation strains are cultured, an appropriate seed culturing medium, a fermentation culturing medium and culturing conditions are selected, pilot scale amplification of the above microbial fermentation preparation method is carried out in a fermentation tank, and the temperature, the rotating speed, the pH value and raw material supplementation are regulated to adjust in order to establish the large-scale microbial fermentation preparation method of novel active bleomycin derivatives 6'-dehydroxy-BLMs. The preparation method shortens the fermentation preparation period of the 6'-dehydroxy-BLMs, improves the fermentation yield of the 6'-dehydroxy-BLMs, increases the proportion of the 6'-dehydroxy-BLMs in total metabolites, and is convenient for subsequent separation and purification. The chemical structure of the bleomycin derivatives is represented by a general formula shown in the specification.

The invention discloses a serum or plasma miRNA marker related to diabetes mellitus type 2 and an application thereof. The invention also discloses a diagnosis kit for diabetes mellitus type 2. The marker disclosed by the invention diagnoses fat children from a microscopic view and warns the risk of long-term insulin resistance and diabetes mellitus type 2; the operation is easy, the result is accurate and objective and reliable, the diabetes mellitus type 2 group and the normal metabolism group can be perfectly distinguished, and the risk of long-term insulin resistance and diabetes mellitus type 2 of fat children can be accurately and sensitively evaluated and warned; and only a blood sample is required instead of other tissue samples, thus the possibility and feasibility of clinical application are greatly improved, and an guidance basis is provided for clinical doctors to make early prevention, intervention schemes and judgment prognosis.

The invention discloses a Paecilomyces thermophila mutant strain and a mutation induction method and application thereof. The mutation induction method includes: Paecilomyces thermophila screened from high-temperature-fermented compost and used for producing high-temperature-resistant and acid-resistant glucose oxidase is used as the original strain, protoplast ultraviolet and ethyl methane sulfonate compound mutation induction is performed, and the screened strain is subjected to budding spore diethyl sulfate-microwave compound mutation induction to obtain the Paecilomyces thermophila mutant strain. The Paecilomyces thermophila mutant strain is preserved in China General Microbiological Culture Collection Center on January 11th, 2016, and the preservation number of the mutant strain is CGMCC No. 13182. Compared with a conventional mutation induction method, the mutation induction method has the advantages that the method is high in efficiency and simple in fast in screening, the productivity of the mutant strain is high, the yield of the mutant stain is 277.65% of that of the Paecilomyces thermophila original strain, fermentation liquor enzyme activity reaches 185U/ml, production cost is lowered, and the glucose oxidase produced by the mutant strain is good in high-temperature resistance and acid resistance and can be used as feed enzyme applied to feed industry.

The invention discloses a separation extracting device and method for micro plastic in soil and belongs to the technical field of solid waste treatment. The device comprises a separating system and afiltering system; through a magnetic stirring device, a flotation solution and samples in a separating pond are stirred uniformly; after standing, the samples in the separating pond are layered; at this moment, micro plastic particles, small in density, in the soil samples are suspended in the solution upper layer, namely a non-precipitating layer; heavy sediment settles on the solution bottom layer, namely a precipitating layer; then flotation liquid in a liquid storing tank flows into the upper layer, namely the non-precipitating layer, of the separating pond through a liquid entering pipe;meanwhile, bubbles generated at the position of an aeration head of an aeration pump are utilized for driving the upper layer solution containing the micro plastic to flow into an extraction filteringdevice to be filtered, and therefore the purpose of separating is achieved. The device is simple and efficient, easy to operate, green and economical in separating method and good in separating effect.

The invention provides an extraction and purification method and application of a fermented astragalus polysaccharide. The extraction and purification method of the fermented astragalus polysaccharide comprises the following steps of (1) carrying out warm immersion extraction on fermented astragalus, concentrating an extracting solution by using a membrane intercepting ultrafilter, removing protein through a Sevag method, and carrying out dialysis, alcohol precipitation and washing to obtain a crude fermented astragalus polysaccharide; (2) purifying the fermented astragalus polysaccharide by adopting DEAE Sepharose FastFlow, eluting by using ultrapure water, concentrating and freeze-drying an eluant to obtain a neutral polysaccharide DF-1, eluting by using gradient NaCl, and concentrating, dialyzing, concentrating and freeze-drying the eluant to obtain a weakly acidic polysaccharide; (3) purifying the neutral polysaccharide DF-1 obtained from the step (2) by adopting a Sephacryl S-400 High Reslution preparation chromatographic column, eluting by using ultrapure water, concentrating and freeze-drying the eluant to obtain neutral polysaccharides SF-1 and SF-2. Compared with a crude drug APS, the FAPS (Fermented Astragalus Polysaccharide) extracted by applying the extraction and purification method provided by the invention is outstandingly increased in yield and content.

The invention discloses a single molecular expansion type alkyl phosphinate flame retardant. A structural general formula is shown in the description, a molecular structure is starlike, R-O- is a hydroxyl dehydrogenized structure after a nitrogen-containing polyhydroxy compound is subjected to esterification reaction, the formula (shown in the description) is a hydroxyl removed structure provided with molecular hydroxyl and obtained after organic phosphonic acid esterification reaction, n corresponds to the number of hydroxy and amidogen of the nitrogen-containing polyhydroxy compound, and M is a metal or semi-metal having the valence m. The invention further discloses a preparation method the flame retardant. The three-source proportion of the flame retardant can be regulated and controlled by selecting different nitrogen-containing polyhydroxy compounds. The molecular structure contains P-C keys and has excellent heat stability and good chemical properties. A specific carboxylic ester structure has good mixing compatibility with a polymer, and the original mechanical properties of the polymer can be retained to the greater degree. Metal ions in molecules can prevent the flame retardant from migrating from the polymer and meanwhile have a catalytic charing effect.

The invention relates to an mRNA (messenger ribonucleic acid) quick-extraction kit and a preparation method thereof. The kit comprises a separation device for solid-liquid separation, wherein the separation device at least comprises a layer of cellulose solid-phase carrier. The kit also comprises at least one tube containing a cell or tissue lysis solution, at least one tube containing a biotinylated Oligo (dT) probe, at least one tube containing a combination buffer solution, and at least one tube containing an elution buffer solution. The kit is simple, quick and efficient to operate, and is suitable for separating and extracting mRNA in a biological sample. The invention is mainly characterized in that the separation device uses cellulose as the solid-phase carrier, a fusion protein CBD-SA is used as a biological bridging agent, and a biologically-specific coupling method is used instead of the traditional physical adsorption method and chemical bonding method to activate the cellulose solid-phase carrier, so that the activated solid-phase carrier can be efficiently combined with the biotinylated reagent to be used for separating protein or nucleic acid.

The invention discloses a method for selective extraction separation of trivalent actinides and lanthanides in fuel-short after-treatment. The method is implemented through the steps that NTAamide(n-Oct) serves as an extraction agent, kerosene serves as a diluting agent, a water-soluble ligand TEE-BisDGA serves as a detergent, a hydroxycarboxylic complexing agent or ammoniacarboxylic complexing agent or a nitric acid solution serves as a stripping agent, and a multi-stage extraction technology is utilized, firstly, a large number of actinide elements and part of lanthanide elements are extracted from feed liquid through the extraction agent, then the lanthanide elements in a loaded organic phase are removed through the detergent, finally the actinide elements are stripped from the organicphase through the stripping agent, thus selective extraction separation of the actinide elements and the lanthanide elements can be realized, the recovery rate of the actinide elements exceeds 99%, and the recovery purity can reach 99.9% or above.

The invention relates to a two-enzyme co-expression technique and a two-enzyme coupling and immobilizing technique, and specifically relates to a co-expression preparation method of a site-specific mutant glucose isomerase and a D-allulose 3-epimerase, a coupling and immobilizing technique of the two enzymes and application of the coupled and immobilized two enzymes in producing allulose from jujube condensed juice. In order to solve health problems of people due to high-calorific value sugar, the invention provides a method for promoting the production of low-calorific value D-allulose from the recombinase glucose isomerase and the D-allulose 3-epimerase through co-immobilization and two-enzyme coupling by virtue of a multiple-enzyme coupling and immobilizing technique with D-glucose and D-fructose in the jujube condensed juice as the substrates, and also provides a method for site-specific mutagenesis of an amino acid at the 146th site of the glucose isomerase and co-expression thereof with the D-allulose 3-epimerase.

The invention provides a method for extracting Ti-rich compounds from Ti-containing slag. The method comprises the following steps: first adding some acidic oxide to the Ti-containing slag with the existence of reducing agent, conducting the mixing process in the molten state, and then cooling off the mixture to crystallize to get the Ti-rich compound. The invention takes full advantage of the high-temperature and low-reducibility features of the Ti-containing slag, and can obtain a high-quality Ti-rich compound with fewer impurities. The titanium dioxide content of the Ti compound is over 70 percent, and thus facilitating the separation and extraction of titanium dioxide. The residue after the separation of titanium dioxide can be used to produce slag cement. The invention has the advantages of simple process, easy device, convenient operation and environmental protection. The invention enables the slag's own heat energy to be fully used. And the obtained production contains less impurities.