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Childhood obesity-related serum or plasma miRNA markers and use thereof

A technology for markers and obesity, applied in the field of serum or plasma miRNA markers, can solve the problems that tissue miRNAs cannot meet the needs of early diagnosis, cannot be applied to screening and prevention, etc., achieve accurate, objective and reliable results, and improve the possibility and feasibility , the effect of easy separation and extraction

Active Publication Date: 2016-10-12
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although miRNA in tumor tissue can be used as a new tumor marker, miRNA in tumor tissue must be obtained through surgery or puncture, so tissue miRNA cannot meet the needs of early diagnosis and cannot be used for extensive screening and prevention.

Method used

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  • Childhood obesity-related serum or plasma miRNA markers and use thereof
  • Childhood obesity-related serum or plasma miRNA markers and use thereof
  • Childhood obesity-related serum or plasma miRNA markers and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1. Test materials: Trizol reagent, TaqMan reversal rate kit, Universal MasterMix II was purchased from Life technologies; chloroform and absolute ethanol were purchased from Sinopharm Pharmaceutical Co., Ltd.; DEPC water was purchased from Shanghai Beyontian Biotechnology Co., Ltd.

[0045] 2. Sample collection and sample data collation:

[0046] The present invention uses a total of 352 children's blood samples, including 136 samples of the obese group and 216 samples of the normal group; there is no significant difference in age between the two groups of samples (P>0.05), and there is a significant difference in BMI (P<0.001). There were significant differences in blood lipid, total cholesterol, low-density lipoprotein and high-density lipoprotein (P<0.05). Fifteen serum samples from obese children and 15 normal children were selected respectively. After total RNA was extracted, every 5 samples were mixed into one sample for deep sequencing analysis (Deep sequencing...

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PUM

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Abstract

The invention discloses childhood obesity-related serum or plasma miRNA markers and a use thereof. The markers comprise one or more of miR-26b, miR-222, miR-146a and miR-146b. The invention also provides a childhood obesity early diagnosis kit. The markers can diagnose childhood obesity on the micro-level and provide early warning of a childhood obesity risk. The markers can be operated simply, produce accurate, objective and reliable results, can distinguish an obese group and a normal group, can accurately and sensitively evaluate childhood obesity and provide early warning of a childhood obesity risk, prevent use of a blood sample and other tissue samples, greatly improve the possibility and feasibility of clinical application and provide guidance and basis for clinicians to make early prevention and intervention schemes.

Description

technical field [0001] The invention relates to the fields of genetic engineering and medicine, in particular to a serum or plasma miRNA marker related to childhood obesity and its application. Background technique [0002] With the prosperity of the country, the living standards of our residents are increasing day by day, especially since the reform and opening up, people's lifestyles have also undergone tremendous changes. The improvement of the economic level and the acceleration of the mechanization process have led to a decrease in the labor intensity and physical activity level of the residents, and the improvement of the living standard has led to a high-fat and low-carbohydrate diet; the incidence of obesity has rapidly increased among various urban and rural populations, becoming a serious disease. Epidemics that endanger the health of residents. The hazards of the obesity epidemic are increasingly recognized by the general public. Obesity can not only lead to card...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 郭锡熔尤梁惠季晨博崔县伟祝丽君李沄
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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