The invention discloses a production method for microbial germ powder, which has the technical steps that: the heat treatment is carried out on proper production seeds after primary seed culture at the treatment temperature being 60 to 90 DEG C for 5 to 60 minutes, the heat treatment is carried out after secondary seed culture, the heat treatment and the cold treatment are carried out after tertiary seed culture, then, the materials are inoculated into a liquid culture medium and is cultured for 16 to 36 hours in a fermentation tank, a liquid culture medium and/or alkali are/is supplemented into the fermentation tank in real time according to the real-time data dynamically detected in the fermentation culture process, germ sledge is obtained after fermentation liquid is separated, the germ sledge is uniformly mixed with absorbing agents, and germ powder is obtained after the crushing and the sieving. The method has the advantages that the viable count of the fermentation liquid is greatly improved, the fermentation factor and the yield are high, the energy consumption is low, the cost is low, the product quality standard is improved, microbes realize the synchronously growth in the fermentation culture process of the seeds after the heat treatment and cold treatment, the viable count of the obtained germ powder is high, the spore mature speed is high, the spore conversion rate is high, the fermentation level is high, and the viable germ yield and the survival rate are high.