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Respiratory type solid-state fermentation method and fermentation tank

A solid-state fermentation and fermenter technology, applied in solid-phase fermentation bioreactors, fermentation, bioreactor/fermenter combinations, etc., can solve the problems of controversial impact on production efficiency, reduced production efficiency, and impact on growth and metabolism. Achieve the effect of improving fermentation level, increasing evaporation and increasing overall reliability

Inactive Publication Date: 2011-09-21
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the impact of stirring on production efficiency is controversial
Some literature reports: the effect of the improvement of mass transfer and heat transfer performance caused by stirring is better than the adverse effects caused by stirring; other studies have shown that stirring will reduce production efficiency, and stirring poses a challenge to solid-state fermentation systems. additional requirements
First, the solid substrate should be able to withstand shearing, and there should be no tendency to agglomerate due to stirring; second, stirring will damage the mycelia, which may affect its growth and metabolism

Method used

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  • Respiratory type solid-state fermentation method and fermentation tank
  • Respiratory type solid-state fermentation method and fermentation tank
  • Respiratory type solid-state fermentation method and fermentation tank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Production of cellulase by fermenting Trichoderma reesei in a breathing solid-state fermenter.

[0025] A fermenter with a height-to-diameter ratio of 1:2, a diameter of 1.5m, and a cylinder height of 3m is used, with a volume of 5.3m 3 . The taper angle at the bottom of the tank is chosen to be 150°, and the taper volume is 0.118m 3 , can be used to store a certain quality of sterile water for air humidity regulation. The height of the tray is 15cm, and the number of tray layers is 20. The used substratum of solid-state fermentation is steam-exploded corn stalk and wheat bran, and its mass ratio is 4: 1, and the inorganic salt solution of 2.5 times (mass ratio) is added after the two are thoroughly mixed, and the composition of inorganic salt solution is: (NH 4 ) 2 SO 4 , 15g / L; MgSO 4 , 6g / L; KH 2 PO 4 , 3g / L. The culture medium was sterilized at 121°C in a steaming tank for 40 minutes, and then inoculated with Trichoderma viride liquid seeds, and the inoculu...

Embodiment 2

[0029] Fermentation of Xanthomonas campestris CGMCC 1.1781 to produce xanthan gum in a breathing solid-state fermenter.

[0030] A fermenter with a height-to-diameter ratio of 1:1, a diameter of 2m, and a cylinder height of 2m is used, with a volume of 6.28m 3 . The taper angle at the bottom of the tank is chosen to be 120°, and the taper volume is 0.524m 3 , can be used to store a certain quality of medium. The height of the tray is 10cm, and the number of tray layers is 20. The medium used for solid-state fermentation is glucose 30g L -1 , NH 4 NO 3 1g L -1 , yeast powder 2g L -1 , MgSO 4 ·7H 2 O 0.25g L -1 、Na 2 HPO 4 0.1gL -1 , CaCO 3 3g L -1 .

[0031] Load the polyurethane foam block that side length is 0.5cm in the feed tray, and the height of packing is 6cm, and the space of 4cm is reserved on the top of the feed tray.

[0032] Open the tank cover 27, and the tray is hoisted into the reactor through the tray cross 25. Close the tank cover 27, feed sat...

Embodiment 3

[0034] Clarithromycin is produced by continuous solid-state fermentation of Streptomyces clavuligerus in the present invention.

[0035] A fermenter with a height-to-diameter ratio of 1:5, a diameter of 1m, and a cylinder height of 5m is used, with a volume of 3.93m 3 . The taper angle at the bottom of the tank is chosen to be 60°, and the taper volume is 0.453m 3, can be used to store a certain quality of medium. The height of the tray is 50cm, and the number of tray layers is 10. The medium used for solid-state fermentation is glycerol 12g / L, soybean flour extract 300ml / L, peptone 10g / L and KH 2 PO 4 0.8g / L.

[0036] Loading side length in the feed tray is a polyurethane foam block of 1cm, the height of the tray is 45cm, and there is a 5cm space on the top of the reserve tray.

[0037] The operation mode of the reactor for continuous fermentation is as follows: open the tank cover 27, and lift the tray through the tray cross 25 into the reactor. Close the tank cover 2...

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Abstract

The invention discloses a respiratory type solid-state fermentation method and a fermentation tank. The fermentation tank structurally comprises double pressure-bearable tank bodies, a respiratory type air charging and discharging system and a liquid circulating system. In a fermentation process, the respiratory type air charging and discharging system is used for alternatively sucking an air part form one tank body and charging the air part into the other tank body, then the fermentation tank undergoing air suction sucks in fresh air, the tank body undergoing air charging rapidly discharges air, and thus, the two tank bodies charge and discharge air alternatively. The design disclosed by the invention ensures that waste air in the tank bodies can be completely discharged, and the evaporation of moisture in a culture medium can be accelerated and the heat generated in the fermentation process can be taken way in time in an air discharging process. Microorganisms are periodically stimulated due to the periodical changes of internal pressure of a reactor, so that the growth of the microorganisms is favorably promoted.

Description

technical field [0001] The invention belongs to the field of biochemical industry, in particular to a breathing solid-state fermentation method and a fermenter. Background technique [0002] Solid-state fermentation (Solid-state fermentation, SSF) refers to one or more microbial fermentation processes carried out in the state of no or almost no free flowing water, although the medium is solid and rich in water. Since the 1940s, submerged fermentation has been a protagonist in the fermentation industry. With the development of the times, the further development of liquid submerged fermentation technology has encountered obstacles. High pollution and high energy consumption are the biggest problems it faces. High-concentration and high-viscosity fermentation has become an important development direction of liquid submerged fermentation. However, high-concentration and high-viscosity fermentation The ultimate limit state is solid-state fermentation. [0003] From the point of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C12M1/00C12M1/36
CPCC12M21/16C12M27/22C12M41/40C12M23/56
Inventor 陈洪章李宏强
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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