258 results about "Solid-state fermentation" patented technology

The invention discloses a traditional Chinese medicine residue organic compound fertilizer special for acid-base imbalance barren soil and a production method, and relates to the technical field of soil improvement and fertilizer.The organic compound fertilizer is prepared from, by volume, 50-70 parts of fermented traditional Chinese medicine residue matrix, 15-30 parts of mineral nutrient unit, 5-15 parts of biological activity enhancer and 3-10 parts of structure conditioner; wherein the fermented traditional Chinese medicine residue matrix is prepared by taking traditional Chinese medicine pharmacy mixed residues as raw materials and performing solid-state fermentation through a complex microbial inoculant containing aspergillus niger and the like; the mineral nutrient unit is compounded by urea and the like; the biological activity enhancer contains humic acid and the like; the structure conditioner is a mixture of attapulgite and sodium carboxymethyl cellulose (5: 1-8: 1). According to the organic compound fertilizer, the fermented traditional Chinese medicine residues with the organic matter content larger than or equal to 45% serve as a matrix, the mineral nutrient unit, the biological activity enhancer and the structural conditioner which are coated in a slow-release mode are matched, a synergistic system is formed, three-stage fermentation is adopted, the microbial activity is kept, and the pH lt can be improved; 6.0 or gt; 8.5 of sand / clayey barren soil.

The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus capable of inhibiting growth of monascus and application of the plant lactobacillus. The preservation number of the plant lactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.34851. According to the present invention, the plant lactobacillus CCNH295 has strong bacterial inhibition ability on common soybean meal contaminating molds, such as aspergillus flavus, aspergillus ochraceus, fusarium moniliforme and penicillium; the plant lactobacillus CCNH295 has excellent bacteriostatic ability to monascus, and when the plant lactobacillus CCNH295 is used for fermentation production of soybean meal, growth of monascus can be remarkably inhibited, so that mildewing of the fermented soybean meal is effectively inhibited, and the prepared fermented soybean meal does not mildew after being placed at 37 DEG C for 6 months; besides, the fermented soybean meal produced by solid state fermentation of the plant lactobacillus CCNH295 is used as a feed raw material, so that the stability of the feed can be improved, and the palatability of the feed can be improved.

The invention provides lactobacillus reuteri GY-18 and application thereof in increasing the content of astragaloside, and belongs to the technical field of microorganisms, fermentation and natural product biotransformation. The lactobacillus reuteri GY-18 capable of improving the content of astragaloside in a fermented astragalus membranaceus product is obtained through screening, the lactobacillus reuteri GY-18 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20252796, and the lactobacillus reuteri GY-18 can be used for improving the content of astragaloside in the fermented astragalus membranaceus product. The lactobacillus reuteri GY-18 can secrete acetylxylan esterase for promoting conversion of astragaloside into astragaloside IV, has efficient specificity on conversion of astragaloside into astragaloside IV, and is high in conversion capacity; according to the method, the content of astragaloside IV is remarkably increased within 24 hours in a semi-solid state fermentation mode, and the method has good practicability.

The invention relates to the technical field of resources and environment, in particular to a method for pretreating lignocellulose by using a rumen microorganism dry method. The method comprises the following steps: crushing lignocellulose to 1-5cm, and filling the crushed lignocellulose into a sealed space; spraying microorganisms under an anaerobic condition according to a mass ratio of rumen microorganisms to lignocellulose volatile components of (0.015-0.03): 1, and controlling the water content of the system to be 30-60%; the pretreatment temperature is regulated and controlled to be 35-42 DEG C, the pH is 6.0-7.5, the pretreatment time is 10-14 days, so that the total abundance of the functional microorganisms Pseudocidovorax, Hallella and Prevotella is greater than or equal to 55%, and the three types of microorganisms respectively form strong interaction with specific companion bacteria; when the relative crystallinity of the lignocellulose is reduced by more than 15% and the volatile component is reduced by 10%, finishing the pretreatment; and performing process regulation and control according to the crystallinity and the reducing sugar yield when the pretreatment is performed for 10 days. The dry process is adopted, the bionic principle and the solid-state fermentation technology are combined, all-component gradient utilization of lignocellulose is achieved, a green solution is provided for industrial and high-value conversion of lignocellulose, and remarkable economic, social and environment-friendly benefits are achieved.

The invention relates to the technical field of biology, in particular to a complex microbial inoculant and a method for improving quality and enhancing aroma of flue-cured tobacco by using the complex microbial inoculant, the tobacco is inoculated with the microbial inoculant before and after curing or in a re-drying link, and short-period solid state fermentation is carried out under controllable temperature and humidity conditions, so that the generation and conversion of aroma precursors in the tobacco are promoted, and the quality and aroma of the flue-cured tobacco are improved. The method has the advantages that pleasant aroma substances such as flower fragrance and fruit fragrance are directionally improved, fragrance fineness and harmony are improved, stimulation and offensive odor are reduced, and accordingly, stable improvement of flue-cured tobacco raw material flavor level and high-end formula adaptability is realized on the premise of not depending on a large amount of additional flavors and fragrances and being compatible with an existing processing technology.

The present application belongs to the technical field of microbial fermentation, and particularly relates to a strain of Kroppenstedtii ivory color and application thereof. In order to provide more high-temperature-resistant and pyrazine-producing brewing microbial resources, a new strain of Kroppenstedtii ivory color MZ35 is isolated from Maotai-flavor Daqu, and the preservation number is CCTCC NO: M 20252598. The strain can adapt to the solid-state fermentation environment of higher temperature and the pile fermentation environment of Maotai-flavor liquor, and promote the formation of tetramethylpyrazine. After MZ35 is inoculated into the solid-state fermentation system of highland barley or the intensified fermentation system of wine lees, the content of tetramethylpyrazine can be significantly increased. In addition, the strain can also promote the accumulation of acetoin, trimethylpyrazine and other Maotai-flavor related substances or precursor substances. Therefore, the strain has important application value for the pile fermentation of liquor, the flavor regulation of wine lees and the preparation of solid-state fermentation products rich in tetramethylpyrazine.

This invention discloses a fermentation process and application of wheat germ, belonging to the field of feed additive technology. The fermentation process includes the following steps: mixing Bacillus coagulans powder (accession number CGMCC No. 1.17145), Lactobacillus salivarius powder (accession number CCTCC NO: M 2022848), and steam-sterilized and cooled wheat germ; performing solid-state fermentation in a sealed environment; and then sterilizing with ozone after fermentation to obtain fermented wheat germ. This invention produces highly nutritious fermented wheat germ through simple steps. This fermented wheat germ can be used as a feed additive for laying hens, improving their production performance.

The invention relates to the technical field of solid state fermentation, and discloses fermentation equipment which comprises a box body, partition plates are transversely arranged in the box body and divide the interior of the box body into a plurality of fermentation layers, and a left side plate and a right side plate of each fermentation layer are each provided with an air inlet connector and an air outlet connector; the fermentation bags are arranged in the fermentation layers, air inlets and air outlets are formed in the fermentation bags, the air inlets are connected with the inner ends of the air inlet connectors of the corresponding fermentation layers, and the air outlets are connected with the inner ends of the air outlet connectors of the corresponding fermentation layers; a first cavity is formed in the ventilation component, the first cavity is communicated with the outer ends of all the air inlet connectors, and an air source connector communicated with the first cavity is formed in the ventilation component. The invention also provides a fermentation system. The fermentation equipment disclosed by the invention has the functions of aerobic fermentation and anaerobic fermentation, can conveniently switch between the aerobic fermentation and the anaerobic fermentation, and avoids the problems of poor air permeability and overlarge pressure in the fermentation bag.

Provided are an Aspergillus niger, a microbial agent thereof, a solid-state fermentation broth thereof, a preparation method therefor, and a use thereof. The Aspergillus niger is Aspergillus niger AGM01, which is preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: M2024768. The Aspergillus niger has a relatively high enzyme activity of β-glucosidase. By performing fermentation and cultivation on the Aspergillus niger in a solid-state fermentation medium containing a small-molecule inducer so as to obtain a solid-state fermentation broth of the Aspergillus niger, the enzyme activity of β-glucosidase in the solid-state fermentation broth of the Aspergillus niger is improved.

The invention relates to the field of environmental pollution treatment and microorganisms, and discloses a preparation method of a trichoderma asperellum straw fermentation extracting solution and application of the trichoderma asperellum straw fermentation extracting solution in oxytetracycline pollution remediation or crop growth promotion. The method comprises the following steps: 1) performing solid-state fermentation on a trichoderma asperellum T1 seed solution to obtain a fermentation product; and 2) drying the fermentation product, adding the dried fermentation product into the leaching solution for leaching, and filtering to obtain the trichoderma asperellum straw fermentation extracting solution rich in trichoderma asperellum spores, enzyme and reducing sugar. The invention finds that the trichoderma asperellum T-1 has new application of repairing oxytetracycline pollution and promoting crop growth, can efficiently degrade oxytetracycline at normal temperature especially in the aspect of repairing oxytetracycline pollution, and has practical application value. Furthermore, compared with a non-extracted trichoderma asperellum leavening and pure trichoderma spores, the trichoderma asperellum straw fermentation extracting solution obtained by a specific process has a better oxytetracycline pollution repairing effect and a better crop growth promoting effect respectively in comparison with the non-extracted trichoderma asperellum leavening and the pure trichoderma spores.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

The invention relates to the technical field of biological waste treatment, in particular to a preparation method and application of a silkworm sand feed additive. The invention provides a technical scheme for preparing a silkworm and mulberry sand feed additive rich in acid protease, pectinase and cellulase by taking silkworm sand as a core matrix and performing directional pretreatment, adaptive strain fermentation and standardized post-treatment. According to the invention, silkworm sand-corncob powder is used as a composite substrate, and the silkworm sand and the corncob powder complement each other in function, so that the problems of unbalanced nutrition and poor air permeability of a single substrate are solved. Specific aspergillus niger is used for segmented solid-state fermentation, parameters are set in a differentiated mode, and full-amount utilization of the silkworm sand is achieved; the product quality and enzyme activity are improved by optimizing the process, and the resource value is improved from the low-added-value field to the high-added-value enzyme preparation industry.

This invention discloses a method for brewing a light-aroma baijiu using a mung bean compound koji starter and its preparation, belonging to the field of baijiu brewing technology. The method uses 75%-80% red sorghum and 20%-25% mung beans as brewing raw materials. First, the mung beans are dehulled, crushed, and pre-treated by low-temperature steaming, then mixed with sorghum and steamed again for gelatinization. Separately, a mung bean compound koji starter inoculated with Rhizopus, Aspergillus oryzae, and ester-producing yeasts is prepared separately and blended with a light-aroma low-temperature koji starter at a ratio of 1:2-3, with the total koji amount being 19%-23% of the grain input. The mixture is then fermented in earthen vats for 28 days in a solid-state environment, with a peak temperature of 28-32℃ and an outlet temperature of 20-25℃. The middle section of the liquor is distilled, yielding 65% vol, and aged in earthenware jars for 3-6 months. This invention constructs a homologous fermentation system of "mung bean koji making—mung bean brewing—koji base," selectively enriching proteases and esterases, solving the problem of insufficient mung bean protein conversion. The finished liquor has a rich, light mung bean aroma and a mellow, sweet taste.

The invention discloses a selenium-rich rice mineral source soil conditioner and a preparation method thereof, and belongs to the technical field of soil conditioning materials. The conditioner comprises the following raw materials in parts by weight: 30-70 parts of biological activated yellow phosphorus slag, 1-1.5 parts of soluble selenium, 23-62 parts of an activating agent and 3-10 parts of a modifying agent. The method is characterized in that the biological activated yellow phosphorus slag is prepared by sequentially carrying out thermal activation and acid activation on yellow phosphorus slag and then carrying out solid-state fermentation by utilizing a compound microbial agent consisting of paenibacillus mucilaginosus, lactobacillus acidophilus and P.megatherium. Through unique biological activation modification, the industrial problems that a mineral source conditioner is low in granulation rate and slow in particle hardening and disintegration are solved in a synergistic mode, acid soil can be effectively improved, absorption and enrichment of rice on selenium are remarkably promoted, and high-added-value resource utilization of the yellow phosphorus slag is achieved.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition and a preparation method and application thereof, active ingredients of the traditional Chinese medicine composition including a fermentation product prepared by subjecting traditional Chinese medicine raw materials to anaerobic solid-state fermentation in combination with Lactobacillus helveticus and enzyme preparation; the traditional Chinese medicine raw materials include ginseng, poria cocos, Chinese yam, white lentil, lotus seed, yiyi seed, amomum villosum, platycodon grandiflorum, licorice and dried tangerine or orange peel; and the enzyme preparation includes cellulase and / or cellobiase. The application effectively promotes dissolution of effective ingredients of Shenlingbaizhu powder, significantly enriches antioxidant active ingredients in the fermentation product, relieves inhibition of oxidative stress on immune cells, and greatly improves survival rate and phagocytic efficacy of macrophages, so that the application shows a significant antibacterial pneumonia effect and has an excellent application prospect.

The invention relates to the technical field of microbial fermentation engineering, and particularly discloses natto red yeast rice herb bean flour and a preparation method thereof. The preparation method comprises the following steps: respectively activating specifically preserved bacillus natto and monascus to obtain secondary seed solutions; inoculating the two seed solutions into a solid-state fermentation culture medium for co-fermentation; and inactivating, drying and crushing the fermentation product to obtain a finished product. The solid-state fermentation culture medium is prepared by compounding a bean matrix (soybeans, black beans or chickpeas) and herb tea components (roses, lotus leaves, chrysanthemums, mulberry leaves, haws, cassia seeds and the like). Through the synergistic effect of the bacillus natto and the monascus, nattokinase and monacolin K are efficiently enriched in a single fermentation system at the same time, conversion of soy isoflavone to a high-bioavailability aglycone type is remarkably promoted, and the obtained product has multiple health-care functions of thrombolysis, lipid reduction, efficient absorption and the like.

The application discloses an engineering biogas fermentation device and a method thereof. The fermentation device comprises a liquid fermentation material feeding mechanism and a solid fermentation material feeding mechanism. The liquid fermentation material feeding mechanism comprises a homogenate pool, which is connected with a feeding port of the fermentation device through a liquid inlet pipe. The solid fermentation material feeding mechanism comprises a feeding hopper and a conveying device. The conveying device comprises a motor and a conveying belt driven by the motor. The feeding hopper is arranged on the conveying belt. The solid fermentation material enters the feeding hopper and is fed to the fermentation device by the conveying device as needed. The liquid fermentation material feeding mechanism and the solid fermentation material feeding mechanism cooperate to form a mixed area for feeding in two directions at the bottom of the fermentation device. The application can reduce the methane emission of fermentation materials in the storage link in the station and the energy efficiency loss caused by invalid degradation of the fermentation materials. The application can provide high-quality fermentation raw materials for a biogas project and realize effective quality preservation and emission reduction.

The present application relates to a kind of mung bean skin fermentation and its preparation method and application of reinforced anti-oxidation, anti-aging effect, belong to the field of microbial technology.The present application provides a kind of preparation method of mung bean skin fermentation of reinforced anti-oxidation, anti-aging effect, including the following steps: after being mixed with white aspergillus, mung bean skin containing solid-state fermentation medium is carried out aerobic fermentation, after fermentation is ended, it is sterilized and is treated, and the pretreated mung bean skin is obtained;Bifidobacterium is inoculated into the culture medium containing pretreated mung bean skin anaerobic fermentation, and mung bean skin fermentation is obtained.The present application is by mung bean skin as one of the main components of solid-state fermentation medium and white aspergillus aerobic fermentation, so that the cell wall of mung bean skin is degraded, and reducing sugar and polyphenol are released, are used by bifidobacterium, so that the fermentation efficiency of bifidobacterium is improved, and then the content of active ingredient in mung bean skin fermentation is improved, and mung bean skin fermentation is endowed with excellent anti-oxidation, anti-aging effect.

According to the fermented feed capable of improving the antioxidant capacity of the animal body and the preparation method of the fermented feed, cerium-zirconium-iron composite oxide ceramic microspheres are prepared and applied to the fermentation process, and in solid fermentation, the microspheres, raw materials pretreated by diacetyl tartaric acid monoglyceride and high-activity complex microbial inoculants have a synergistic effect, so that the antioxidant capacity of the animal body is improved. By quenching active oxygen, the fermentation microenvironment is remarkably optimized, metabolism of functional microorganisms and accumulation of products are promoted, the microspheres can be recycled after fermentation, a functional feed product with the antioxidant activity remarkably improved is finally obtained, and unification of material circulation, process synergy and product increment is achieved.

The invention discloses an edible solid-state fermentation product and a preparation method thereof. The preparation method comprises the following steps: culturing microalgae to obtain a microalgae culture, preparing a granular edible substrate, carrying out first fungus culture to obtain a fungus culture, and mixing the microalgae culture and the edible substrate to form an edible solid-state culture medium. And then, adding the fungus culture into the edible solid culture medium, and carrying out second fungus culture to form an edible solid fermentation product. And finally, performing drying treatment to form edible solid-state fermentation powder containing rich fungi and metabolites thereof, and microalgae and metabolites thereof. The edible solid fermentation product provided by the invention maintains nutritional ingredients of fungi and microalgae to the greatest extent, and cell walls of the microalgae are decomposed through the fungi, so that more microalgae metabolites are generated.

The method comprises the following steps: by taking baphicacanthus cusia leaves, baphicacanthus cusia stems and whole baphicacanthus cusia plants as raw materials, sealing, vacuumizing or carrying out nitrogen-filled solid-state fermentation, and extracting with water and an organic solvent to prepare a crude indigo extract; according to the method, the indigo can be efficiently fermented and converted, and the content of indigo in the prepared indigo crude extract reaches 45% or above. The high-purity indigo extract is prepared through purification of different types of ceramic membranes with different molecular weight cutoff and ultrafiltration hollow fiber membranes and extraction and refining of different solvents, the purity of indigo in the indigo extract is higher than 85%, and theoretical reference and technical support are provided for development and application of high-grade dyes and drugs.

The present application relates to a kind of preparation method of barley and gastrodia elata beverage, belong to food processing technical field.The existing gastrodia elata beverage preparation method has the problems such as low active ingredient extraction efficiency, difficult to synergistically utilize barley functional components, flavor relies on exogenous addition, product is easy to precipitate etc..The present application is by the specific proportion of mixing maturation of barley and gastrodia elata, inoculation includes saccharomyces cerevisiae, lactobacillus plantarum and streptococcus thermophilus Compound starter carries out solid state fermentation, then it is by the compound enzyme preparation including alpha-amylase, beta-glucanase and pectinase enzymolysis and subsequent processing, and beverage is prepared.The method synergistically improves gastrodia elata element and barley beta-glucan enrichment, creates coordinated flavor, and improves product stability.The obtained beverage is mainly used to provide the flavor beverage with the nutritional and health care function of gastrodia elata and barley.

The invention relates to saccharomyces cerevisiae MBPSc22 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc22 for synthesizing an immune nutrient, namely cantharidin, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc22 is CGMCC No. 35982; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, and more importantly, the fermented soybean meal is rich in cantharidin, so that the soybean meal protein obtains new functional immune nutrition; therefore, the whole nutritional value of the soybean meal is improved, and the method reaches a leading level in the field of developing functional fermented soybean meal by using saccharomyces cerevisiae.

The application discloses a plant-source bio-growth-promoting synergistic fertilizer and a preparation method thereof. The fertilizer is prepared by compounding plant-source composite growth-promoting substances, a composite microbial flora and a carrier material in a specific mass ratio. The plant-source composite growth-promoting substances are prepared by solid-state fermentation and extraction of gynostemma pentaphyllum, astragalus and acanthopanax. The composite microbial flora contains brazilian nitrogen-fixing spirillum, fluorescent pseudomonas and brevibacillus brevis, and is embedded and fixed by using kappa-carrageenan microcapsules containing biochar nanoparticles. The carrier is lignosulfonate-modified nano-attapulgite. The application also provides a corresponding preparation method. The granular fertilizer is finally prepared through steps of activating the strains by a composite activation solution, embedding by microencapsulation and carrier loading granulation. The fertilizer combines the synergistic effect of plant-source active substances and functional microorganisms, significantly improves the activity of the flora and the field colonization capacity, and can effectively promote plant growth, improve crop yield and quality.

The invention discloses a preparation process of a composition based on corn straw, corn stigma and grain juice, which comprises the following steps: solid state fermentation, liquid state fermentation, low temperature distillation, blending and filling to obtain the grain juice composition, the formaldehyde content of the grain juice composition is lower than 0.5 mg / L, and the grain juice composition is rich in corn peptide. According to the composition based on the corn straw, the corn stigma and the grain juice and the preparation process of the composition, the corn straw and the corn stigma are subjected to optimized solid-state and liquid-state fermentation respectively, and a specific low-temperature distillation technology is combined, so that efficient and mild extraction and conversion of nutritional ingredients and active substances in the raw materials are realized; according to the process, the content of bad byproducts such as formaldehyde in a final product is remarkably reduced, and meanwhile, rich natural active ingredients derived from raw materials are reserved to the greatest extent.

Provided are an Aspergillus niger, a microbial agent thereof, a solid-state fermentation broth thereof, a preparation method therefor, and a use thereof. The Aspergillus niger is Aspergillus niger AGM01, which is preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: M2024768. The Aspergillus niger has a relatively high enzyme activity of β-glucosidase. By performing fermentation and cultivation on the Aspergillus niger in a solid-state fermentation medium containing a small-molecule inducer so as to obtain a solid-state fermentation broth of the Aspergillus niger, the enzyme activity of β-glucosidase in the solid-state fermentation broth of the Aspergillus niger is improved.

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.

The present disclosure relates to the technical field of pharmaceutical preparation, and particularly relates to a preparation method of glabriden; the preparation method comprises the following steps: providing licorice raw materials, crushing and sieving, and then performing a solid state fermentation process to obtain licorice powder after solid state fermentation; then adding into a first mixed organic solution for soaking, and then adding into a first mixed aqueous solution for soaking, and then adding a second mixed organic solution for extraction, collecting the organic phase, and then adding the solid product into the second mixed organic solution for extraction after enzyme inactivation and impurity removal; combining the organic phases and performing dialysis, purification and concentration to obtain a licorice flavone extract; and then performing extraction of the licorice flavone extract by a third mixed organic solution containing imidazolyl sulfonate to obtain a product containing a higher proportion of glabriden.

This invention provides the development of low-GI healthy snacks based on Cordyceps militaris solid-state fermented wheat, belonging to the field of cereal functional food and fermented food processing technology. It includes inoculating Cordyceps militaris strains into the wheat-based material to be fermented, culturing it under solid-state fermentation conditions to obtain fermented wheat-based material. This low-GI healthy snack development based on Cordyceps militaris solid-state fermented wheat uses wheat-based cereal material as the processing object, linking Cordyceps militaris solid-state fermentation, drying, puffing, blending, and shaping processes to construct a processing route for directly preparing low-GI healthy snacks from fermented wheat. This transforms Cordyceps militaris solid-state fermented wheat from intermediate fermentation material into a directly edible end product, achieving an estimated glycemic index of no more than 55 in vitro. This solves the problem in existing technologies where Cordyceps militaris solid-state fermented wheat is difficult to directly form product solutions corresponding to the low-GI snack development goals.

The invention discloses a solid-state fermentation method of penicillium oxalicum, a penicillium oxalicum complex microbial inoculant and application of the penicillium oxalicum complex microbial inoculant in promoting colonization of the penicillium oxalicum in soil and improving available phosphorus in the soil. The solid-state fermentation method comprises the following steps: mixing a penicillium oxalicum spore suspension and a fermentation substrate for fermentation at 30 + / -0.5 DEG C; the fermentation substrate comprises waste flammulina velutipes residues and wheat bran in a mass ratio of 1: 1. The Penicillium oxalicum complex microbial inoculant comprises the following components in parts by mass: 10-25 parts of a Penicillium oxalicum solid-state fermentation product obtained by a solid-state fermentation method, 20-25 parts of diatomite, 20-30 parts of zeolite powder, 5-10 parts of potassium fulvate and 10-15 parts of humic acid.