2450 results about "Solid-state fermentation" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg / g, the reducing sugar reaches 20-80mg / g, the GABA reaches 0.317-0.5mg / g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu / g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU / g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.

A method is provided for manufacturing coffee by solid state fermentation, including the following steps: depositing coffee beans in a dust-free clean container; propagating a fungus with solid state fermentation; carrying out implantation process, wherein the fungus is implanted to the coffee beans contained in the dust-free clean container with a sterile operation, the fungus being one belonging to Eumycota, including at least one selected from Basidiomycotina and Ascomycotina; and performing a fermentation process. Also provided is a formula of the solid cultivation medium used in the method, which is suitable for both large-scale and small-scale production of fungi and is applicable to most fungi to not only increase the throughput as desired, but also provide a metabolic product containing pharmacologically active ingredients.

The invention discloses a production method of a pig feed additive containing traditional Chinese medicine and active probiotics, which comprises the steps of: respectively conducting single-spawn strain quantity expanding culture to the parent strains of active probiotics for pigs; mixing equiponderant first-order seed liquid of all kinds of active probiotics for pigs, inoculating the liquid into seed tank culture mediums for multi-spawn mixed culture and then inoculating the liquid into traditional Chinese medicine liquid culture mediums for primary traditional Chinese medicine fermentation; and mixing multi-spawn mixed traditional Chinese medicine fermentation liquid with corn cob meal with the same volume to obtain solid fermented seeds and then inoculating the seeds into traditional Chinese medicine solid culture mediums for secondary traditional Chinese medicine fermentation. By implementing the embodiment of the invention, the strain activity of the active probiotics in the pigfeed additive and the colonization performance in intestinal canals can be greatly improved, the produced pig feed additive is rich in traditional Chinese medicine compositions, the effect of active compositions which take a pharmacological effect in the traditional Chinese medicine compositions can be fully exerted, the body strengthening and disease curing performance and the growth promoting performance of the pig feed additive are improved and therefore the use of antibiotics can be fully avoided.

The invention relates to the field of microbial fermentation, in particular to a preparation method of a compound Daqu, so as to solve the problems of the traditional Daqu such as single nutritional components of raw materials, longer starter-making and brewing time, low utilization rate of grains, high cost and the like. The preparation method comprises the following steps: (1) treating the raw materials of the Daqu; (2) inoculating aspergillus niger 41254, aspergillus niger UV-11, monascus, mucor racemosus, actinomucor elegans, rhizopus oryzae, saccharomyces cerevisiae, candida drusei, acetobacter, bacillus subtilis and lactobacillus delbrueckii 6100 into a conical flask containing the raw materials of the Daqu for culturing, then expanding the culture and obtaining a mother culture; and(3) adopting uncooked materials for making a starter, inoculating the obtained mother culture and the raw materials of the Daqu into a solid fermentation device, keeping the temperature at 30 DEG C-33 DEG C, continuing for 30-33h, discharging the starter and finally carrying out low-temperature vacuum drying. The preparation method enriches the nutritional components in the raw materials and improves the quality of a brewed product; and a plurality of bacterial strain are artificially added, thereby improving the utilization rate of the grains, shortening the starter-making cycle, improving the yield of the brewed product and reducing the production cost.

This invention relates to a method and system of preparing ethanol based on the solid state fermentation of sweet sorghum stem, belonging to the ethanol production technology of solid state fermentation. It includes the following key processes and device: raw materials smash is to choose the suitable mill to smash sweet sorghum stalk, strain addition is to add ethanol bacteria at the entrance of solid-state fermentation tank, solid-state fermentation is that the raw materials added bacteria is fermented in the slant rotor solid-state fermentation tank, solid distillation is to distill the solid-state fermented materials to obtain ethanol.

A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities. Cordyceps sinensis is preferably grown to produce the active compound H1A which is a derivative of ergosterol.

The invention relates to a nonreactive fermented feed of mixed bacteria and a preparation method thereof, which belong to the field of microbial fermented feed and solid feed. The nonreactive fermented feed is manufactured by mixing and fermenting solution of the mixed bacteria and dried matrix of compound feed and adding 4% of premix into the fermented solution after the fermentation. The fermented feed preparation technology is simple and convenient to operate and can be operated by various farmers and feed manufactures easily. The prepared solid feed is obvious in effect, capable of improving utilization of feed and promoting growth of animals, and capable of improving and maintaining balancing of intestinal flora, improving immunity and premonition of the animals, effectively improving body shape of pigs and increasing economic benefits. No antibiotic is added in the preparation process and finial products, the nonreactive fermented feed of the mixed bacteria and the preparation method thereof are healthy, safe and can be directly used in breeding of growing-finishing pigs.

The invention provides a method for producing cordyceps militaris fermentation rice (cordyceps rice for short) by utilizing solid state fermentation of rice, and the method is characterized in that the rice is taken as a main fermentation substrate; and the cordyceps mycelia grows in the rice through a solid state fermentation manner so as to obtain the rice rich in the cordyceps mycelia. The method comprises the following steps: 1. preparing a cordyceps militaris liquid strain; 2. preparing a rice solid state fermentation culture medium; and 3. inoculating and fermenting. In the method, the medical effect of the cordyceps militaris is organically combined with the nutrition of the rice, thus the product contains various nutritive components and mineral elements, especially cordycepin andcordycepic acid, wherein, the content of the cordycepin is more than or equal to 600mg / 100g, and the content of the cordycepic acid is more than or equal to 3g / 100g, thereby improving the immunity ofthe organism, inhibiting tumours, preventing ageing, and satisfying the requirements of healthy eating of people.

The invention relates to a preparation method of protein feed in oilseed dregs. Oilseed dregs are used as a main material in which accessories are added and mixed, and the mixed material is preprocessed and mixed with water; seed liquid resurrection is carried out on the sieved strain of high-efficient degradation anti-nutritional factors; in the form of single strain or combination, 2% to 15% of general inoculum concentration of the strains by weight are simultaneously connected with mixture solid fermentation medium after preprocessing on standing at the temperature of 25 to 45 DEG C, mixed and fermented for 24 to 96 hours; after fermentation, the finished products are obtained after drying and dehydration; the raw materials have the following proportions by weight: 40% to 60% of oilseed dregs, 0 to 10% of accessories and 40% to 50% of water. According to the composition, characteristics and nutrient advantages of anti-nutrient substance in oilseed dregs, the method not only can efficiently eliminate anti- nutritional factors in the dregs but also can improve the oligopeptide content and simultaneously maintains higher protein content so that the quality of protein feed can be further advanced and improved.

The present invention describes a bio-based process to produce high quality protein concentrate (HQPC) and lipids by converting plant derived materials into bioavailable protein and lipids via solid state fermentation (SSF) and hybrid-SSF, including the use of such HQPC and lipids so produced as nutrients, including use as a fish meal replacement in aquaculture diets. Also disclosed is a SSF reactor and method of using the reactor.

A preparation method of a microecological culture feed additive belongs to the technical field of livestock and poultry feed additive. A solid fermentation medium comprises 40% of wheat bran, 10% of corn, 9% of soybean meal, 0.5% of urea, 0.1% of potassium dihydrogen phosphate, 0.1% of sodium dihydrogen phosphate, 0.1% of manganese sulfate, 0.2% of calcium chloride and 40% of water. fermentation strains include plant lactobacillus, bacillus subtilis and saccharomyces cerevisiae in a ratio of 1:2:2. The method comprises the following steps: crushing, sieving, mixing, disinfecting, preparing fermentation bacteria liquid, inoculating fermentation strains and obtaining a finished product. The microecological culture feed additive provided by the invention combines functions of nutrition and health care, has no toxic or side-effect or pollution, and can improve antioxidation capability and immunization function of a weaning pigling, increase daily weight gain of the pigling and forage conversion rate, reduce diarrhoea rate, reduce or prevent pigling weaning stress syndrome and substitute antibiotic additive in forage.

The invention discloses a method of making liquor by virtue of solid fermentation with bamboos as fluff. The method comprises the following steps: crushing clean bamboo products into particles with the particle size of 3-10mm, then steaming the particles of the bamboo products for 10-30 minutes, cooling, mixing the particles serving as the fluff, grains and vinasse, and carrying out solid fermentation to brew Fen-flavor bamboo wines, strong-flavor bamboo wines or soy sauce flavor bamboo wines, wherein the bamboo products are one or more of bamboo stems, bamboo leaves, bamboo branches and bamboo rhizome; and after crushing, the particle sizes of the bamboo rhizome and the bamboo stem are 8-10mm, the particle size of the bamboo branch is 5-8mm, and the particle size of the bamboo leaf is 3-5mm. According to the method disclosed by the invention, the bamboo products and the grains are used for brewing according to a certain ratio, so that the grains can be saved by 10-15 percent under the condition of the same yield of liquor; as different parts of the bamboos in different particle sizes are mixed as the fluff for brewing, the traditional hulls can be completely replaced; meanwhile, as the bamboos and the grains form a particle size gradient, fermentation and distillation of the grains are facilitated.

The invention discloses a low-salt liquid state fermented soy sauce production process, comprising the following main processes: preparing koji in a koji-maker machine; mixing the wheat which is fried and smashed with koji, inoculating the mixture in soybean meal and bran which are cooked; preparing finished koji in a koji making disc machine; mixing the finished koji with brine, sending the mixture in a fermenting vat for fermentation; preparing the finished soy sauce through soaking, pouring oil, filtrating, mixing, sterilizing and filling to obtain the finished soy sauce. The soy sauce is characterized in that materials containing zygosaccharomyces Rouxii 2.180 accounting for 1.5-2.5% of the total weight of materials and torulopsis candida 2.202 accounting for 1.5-2.5% of the total weight of materials are added during the medium term of the fermentation process and the raw materials--soybean meal and bran are cooked by high temperature short-time continuous cooking process. Compared with the prior art, the raw material digestion rate can reach 88-90%, the protein utilization rate can reach 83%, the activity of neutral protease of the finished koji can reach 2000mu / g, the enzymesystem is complete, the defects such as long period of high-salt liquid state fermentation (180 days), burnt flavour and heavy bitter taste of low-salt solid state fermentation and the like are overcame and the produced soy sauce is characterized by strong soysauce-like aroma, alcohol-like aroma and ester-like aroma and the like.

The invention belongs to the technical field of microbial fermentation, specifically relates to a method for preparing an oil cake protein feedstuff through low moisture solid state fermentation. The method for preparing the oil cake protein feedstuff through the low moisture solid state fermentation is characterized in that: the method comprises the following steps: 1) preparing a bacterial inoculum: injecting a mixed solution (comprising a bacterial liquid and a sodium alginate solution) in a CaCl2 solution through an injector, washing three times through sterilized physiological saline after curing to obtain a microcapsule bacterial inoculum, wherein a volume ratio of the sodium alginate solution to the CaCl2 solution is 1:2-1:10; 2) mixing and fermenting cake base materials: uniformly mixing and stirring the microcapsule bacterial inoculum, oil cake and water, carrying out standing fermentation for 24-96 hours at a room temperature to obtain a fermented product; 3) adopting one of the following two methods: (1) carrying out drying, wherein the fermented product is dried after completing the fermentation to obtain the oil cake protein feedstuff; (2) directly carrying out vacuum packaging for the fermented product after completing the fermentation to obtain the oil cake protein feedstuff. The method has characteristics of high survival rate and low production cost.

A method for producing probiotic feed for animals by fermenting high-fiber agricultural and sideline products with complex bacteria. , and / or 0-1.0% calcium chloride and / or 0-1.0% magnesium sulfate) are mixed in proportion and then steamed with water; after the steaming is completed, cool to 20-40°C and inoculate Neurostria fortis and Lactobacillus plantarum is solid-state fermented at 20-40°C for 2-5 days; the fermented feed is dried at 40°C-70°C and crushed to obtain a probiotic feed product rich in carotenoids. The method has the advantages of high production efficiency, low cost and good benefit, and while solving the problem of environmental pollution, it also relieves the pressure of serious shortage of energy and protein feed sources.

A marine-derived Bacillus velezensis (Bacillus velezensis) BMF 03 having a deposit number of CCTCC NO: 2017374 is disclosed. BMF 03 strain and its aseptic fermentation broth have inhibitory effect onplant pathogenic fungi, including apple rot fungi, grape white rot fungi, mango anthracnose fungi and so on. The aseptic fermentation broth of BMF 03 strain can be used as an active ingredient to prepare an antibacterial drug, and the bacteria is selected from the group consisting of Keratinobacteria, Bacillus subtilis and Ralstonia solanacearum. The invention also discloses a fermentation methodof aseptic fermentation broth of BMF 03. The invention adopts a plate confrontation method and an Oxford cup method to screen a new marine bacterial strain BMF-03 with strong bacteriostatic effect andwide bacteriostatic spectrum, and adopts strain morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis to determine the species relationship of excellent resistant strains. At that same time, the bacteriostatic substance produce by the strain, the sporulation fermentation medium and the shake flask fermentation conditions were also study, and the promoting effect of the strain fermentation broth and the solid fermentation product on the growth of the cucumber seedling was clarified, which laid a foundation for the application of the strain.

The invention mainly utilizes different grains or forage such as coix seeds, rice, wheat, oat, etc. as the substrate of the solid state fermentation of cordyceps or ganoderma lucidum. After solid-state cultivation, fermentation production containing high polysaccharide can be gained; the fermentation production is dried and milled into powder. In addition, farina and flavoring agent can be added, then cordyceps or ganoderma lucidum coix seed powder, rice powder, wheat powder, or oat powder with rich health protection components such as dietary fiber and polysaccharide can be produced, thereby being convenient for consumers to brew and drink, or forage contained cordyceps or ganoderma lucidum for fed animals to eat can be produced.

The production of functional red rice powder with high color number and without citrinin belongs to the field of red rice product producing technology. The functional red rice powder is produced with monascus strain No. 9901 or 9906 liquid culture seed and grain material culture medium and through inoculation of liquid seed, solid fermentation at 25-32 deg.c temperature and 60-90 % humidity for 2-3 weeks, sterilization, stoving and milling. During the production, liquid fermentation process may be also adopted. The product has color number of 200-600 U / g and content of functional componentMonacolin K 4-12 g / kg with Monacolin K in open ring structure accounting for 70-90 %, and no toxic matter citrinin. The product may be used as food additive or fixing safely.

The invention discloses a method for preparing a cow feed additive containing traditional Chinese medicine active probiotics, comprising the following steps: respectively carrying out amplification culture of the strain number of a single bacterial specie on original strains of active probiotics for cows; mixing primary seed liquors of active probiotics for cows with same weight, inoculating the mixture in a seed tank medium to carry out multiple-strain mixed culture, then inoculating in a traditional Chinese medicine liquid medium to carry out a primary traditional Chinese medicine fermentation; and mixing the multiple-strain mixed traditional Chinese medicine fermentation liquor with isometric cob powder to obtain solid fermentation seeds, and then inoculating in a traditional Chinese medicine solid medium to carry out a secondary traditional Chinese medicine fermentation. According to the invention, the strain activity and colonization performance in the intestinal tracts of activeprobiotics in the cow feed additive are improved greatly, the prepared cow feed additive is rich in traditional Chinese medicine components, the active components with pharmacological effects in the traditional Chinese medicine components fully play the role, thus the performances of strengthening the body, treating diseases and promoting growth of the cow feed additive are improved, and furthermore completely displace antibiotics.

A process for preparing the multi-vitamine multi-enzyme high-protein microbial preparation by the solid fermentation of multiple bacterial strains includes such steps as preparing seed culture media, respectively inoculating reticularia, torula yeast, glutamic corynebacteria, Bacillus subtitis, Aspergillus niger, etc into said seed culture media, culturing at 30-35 deg.C for 15-18 hr, inoculating them into the solid culture medium, fermenting at 33+ / -0.5 deg.C for 70-80 hr, and fast drying. Its can be used as the feed containing living microbes, microbial enzymes, vitamines, etc.

The invention provides a method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds. The method comprises the following steps: on the basis of utilizing the steamed lotus seeds as a worm grass solid state fermentation culture medium, inoculating 3-8ml worm grass liquid strain to per 100 g of the lotus seeds; culturing for 10-30 days at the temperature of 5-30 DEG C and the humidity of 60-90% in dark, namely obtaining worm grass lotus seeds on which hyphae are overgrown; and culturing the worm grass lotus seeds on which the hyphae are overgrown at the temperature of 5-30 DEG C and the humidity of 80-95% for 10-30 days with the illumination of 8-15 hours per day, thus obtaining the worm grass lotus seeds on which worm grass sporocarps are grown. The invention provides a new worm grass source and a new lotus seed health food. According to the invention, the medical effect of worm grass and the nutrition of the lotus seeds are organically combined, so that the product has various nutrient components, is especially rich in active components such as cordycepin, a cordycepic acid, a worm grass polyose and the like and has high edible and medical values; and the worm grass lotus seed fungus preparation process is simple and can easily realize large-scale production, popularization and implementation.

The present invention discloses a compound microbial feed additive. It is a compound microbial feed additive obtain by adding 7 normal microbial pools into solid culture medium and making solid fermentation, drying and pulverizing to obtani powered material. Said 7 liquid micriobial pools are bacillus cereus, peptococcus, lactic acid bacteria, lactobacillus bifidus, yeast, photosynthetic bacteria and actionmycete. In said invented feed additive its viable count is greater than or equal to 180 handred million / gand total number of bacteria is greater than or equal to 240 handred milling / g. Said additive can be aded into animal feed, and can reduce fed-meat ratio and can raise disease resistance of animal.

The invention discloses a solid state fermentation cylinder and a solid state fermentation method. The solid state fermentation cylinder comprises a cylinder body and a jacket provided with an upper port and a lower port, the upper end of the cylinder body is an upper end cover, a mixing system capable of lifting is arranged on the upper end cover, and according to the mixing system, a mixing frame is driven by a driving system. The mixing frame is formed by a wall surface scraper plate, a bottom surface scraper plate and mixing blades, the wall surface scraper plate and the bottom surface scraper plate form a frame-shaped structure, and the mixing blades are fixedly connected in the frame-shaped structure. The solid state fermentation cylinder and the solid state fermentation method effectively resolve the problem of low fermentation performance caused by uneven thickness and uneven heating of a feed layer. According to the solid state fermentation cylinder and the solid state fermentation method, all the processes from feeding to discharging are concentrated in the solid state fermentation cylinder, the process of transportation of materials is simplified, and the mixing system and a cooling thermal-insulation system of equipment are strengthened. Automatic bulk production of solid state fermentation can be achieved, the pure culture in the solid state fermentation process is effectively guaranteed, quality and safety of products are improved, and homogeneity property in the same bulk is guaranteed.

The invention relates to a method for separating ethanol by using Chinese sorghum straw through solid fermentation method and the system, belonging to field of ethanol produ ction process through solid fermentation. It comprises following steps: disintegrating Chinese sorghum straw, solid fermenting and getting fermentation material, conveying it to distilling system through compact channel convey belt; feeding fermentation material into annular rotary disk, contacting with steam from high- efficiency gas distributor to make ethanol converse into gas phase from solid phase, discharging slag continuously; separating ethanol with distilling- rectifying coupled process, getting industrial ethanol of 95.5% (w / w). The ethanol productivity is increased by employing istilling- rectifying coupled process, the ethanol volatilisation is dramatically reduced during conveying process by employing compact channel blet convey; the transiation is improved and continuous production is realized by employing annular rotary disk for solid distillation.

The invention discloses a method and device for preparing fertilizer from kitchen garbage. The method comprises crushing kitchen garbage, carrying out solid-liquid separation, carrying out pyrolysis carbonization on the separated solid kitchen garbage to obtain pyrolysis gas and carbon residue, washing the pyrolysis gas, carrying out anaerobic fermentation on the separated liquid to obtain a broth, biogas residue and biogas, carrying out aerobic fermentation on the broth, then preparing organic-inorganic composite liquid fertilizer from the obtained liquid, mixing the biogas residue and the biogas residue obtained by the pyrolysis carbonization, adding composite microbe engineering bacteria into the mixture, and carrying out solid state fermentation to obtain the organic carbon solid fertilizer. The method and device respectively carry out resourceful treatment on the solid kitchen garbage and the organic liquid, the organic liquid is treated by a biotechnology so that the organic-inorganic composite liquid fertilizer is produced, and solid kitchen garbage is subjected to pyrolysis carbonization so that energy and biochar are produced.

The invention relates to a method for preparing feed protein by mixed fermentation of tilapia leftovers and soybean meals, belonging to the field of feed additive preparation. The method comprises the following steps: mincing fresh tilapia leftovers, mixing the minced tilapia leftovers and crushed soybean meals at 60 meshes, adding water and uniformly mixing, and adding bacteria and enzyme to carry out solid fermentation; and drying and crushing the fermented raw materials to obtain the fermented feed protein. The fermented feed protein powder prepared by the invention is rich in small peptides and probiotics. According to the invention, the protein utilization rate is raised, the growth effect of animal is raised, the survival rate is raised. The fermented feed protein can completely replace fish meal in the feed industry, thus the feed cost is obviously reduced.

The invention provides a tobacco matrix composite biopesticide and a preparation method thereof. The composite biopesticide which comprises at least a tobacco botanical insecticidal component and at least a microbial insecticidal component is prepared through fermenting insecticidal microbes with selecting a tobacco waste as a raw material. The preparation method comprises the following operationsteps: preparation of a medium, amplification culturing of spawns, fermentation of the biopesticide, and extraction. A fermentation technology can adopt liquid state fermentation or solid state fermentation. The tobacco matrix composite biopesticide of the invention which not only contains the insecticidal microbes and metabolites thereof, but also contains tobacco insecticidal components (nicotine and the like for example) has dual insecticidal effects. The method of the invention which allows resources of the tobacco waste which is not reasonably utilized at present to be fully utilized allows grain resources to be saved, and good economic benefits, ecological benefits and social benefits to be generated.

The invention relates to a method for producing a protein feed by using gingko leaf residue as a raw material for mixed strain solid-state fermentation. Activation and multiplication culture are respectively performed on candida tropicalis, aspergillus oryzae, bacillus subtilis and lactobacillus plantarum, so as to obtain four liquid strains; after the gingko leaf residue is dried and smashed, a certain amount of water and a nitrogen source are added, and after mixing and sterilizing, the gingko leaf residue is taken as a solid-state fermentation culture medium; the four liquid strains are inoculated to the solid-state fermentation culture medium according to certain proportions, after sufficient mixing, fermentation for 72 to 120 hours can be performed at a nature pH value under the temperature of 28 to 30 DEG C, and after drying, a gingko leaf residue protein feed can be obtained. In the protein feed, the coarse protein content of the protein feed is high and can reach 46 percent, the nutritional ingredients are complete, the palatability is excellent, the production performance and the feed conversion rate of livestock and poultry can be effectively improved, the preparation method is simple in process, the cost is low, and the mass production can be achieved. The invention further provides a way for using the gingko leaf residue which is a waste material in forestry processing.