444 results about "Solid-state fermentation" patented technology

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention discloses a traditional Chinese medicine residue organic compound fertilizer special for acid-base imbalance barren soil and a production method, and relates to the technical field of soil improvement and fertilizer.The organic compound fertilizer is prepared from, by volume, 50-70 parts of fermented traditional Chinese medicine residue matrix, 15-30 parts of mineral nutrient unit, 5-15 parts of biological activity enhancer and 3-10 parts of structure conditioner; wherein the fermented traditional Chinese medicine residue matrix is prepared by taking traditional Chinese medicine pharmacy mixed residues as raw materials and performing solid-state fermentation through a complex microbial inoculant containing aspergillus niger and the like; the mineral nutrient unit is compounded by urea and the like; the biological activity enhancer contains humic acid and the like; the structure conditioner is a mixture of attapulgite and sodium carboxymethyl cellulose (5: 1-8: 1). According to the organic compound fertilizer, the fermented traditional Chinese medicine residues with the organic matter content larger than or equal to 45% serve as a matrix, the mineral nutrient unit, the biological activity enhancer and the structural conditioner which are coated in a slow-release mode are matched, a synergistic system is formed, three-stage fermentation is adopted, the microbial activity is kept, and the pH lt can be improved; 6.0 or gt; 8.5 of sand / clayey barren soil.

The invention relates to the technical field of sewage biochemical treatment, and discloses a preparation method of an efficient composite biological carbon source, which comprises the following steps: solid-liquid two-phase fermentation treatment, microbial community echelon relay treatment, fermentation liquor reflux and biological detoxification system and biomass enhancement treatment. According to the invention, the preparation of the composite biological carbon source with high concentration (COD > = 50,000 mg / L), high stability (stable operation time > = 120 days) and high activity is realized by combining solid state fermentation and liquid state fermentation, constructing a microbial echelon metabolic network, applying a porous carrier layer to eliminate an inhibitor, implementing active factor regeneration and other technical means; according to the present invention, the organic matter conversion efficiency is improved by more than 30%, the stable operation time of the system is improved, the technical problems of insufficient concentration, unbalanced components, poor stability and the like in the traditional carbon source preparation are solved, the method is suitable for the nitrogen and phosphorus removal process of the large-scale sewage treatment plant, the total nitrogen removal rate of the sewage treatment system can be improved by 15-25%, and the nitrogen removal rate can be improved by 30-40%.

The invention provides high-satiety chicken small molecule peptide and application thereof in chicken meal replacement powder.The method comprises the steps that firstly, intragastric self-swelling microspheres are prepared, wall materials are yeast beta-glucan and hydroxypropyl methyl cellulose, a core material is modified chicken small molecule peptide, the microspheres absorb water to swell in a gastric acid environment to generate satiety, and the self-swelling microspheres absorb water to swell in the gastric acid environment; the modified chicken small molecule peptide is slowly released in an intestinal environment, so that absorption is promoted, and intestinal health is regulated; in addition, the coarse cereal powder is subjected to solid state fermentation by using aspergillus oryzae and then is used for preparing the chicken meal replacement powder, and the self-expanding microspheres in the stomach and the fermented coarse cereal powder are added, so that the satiety is improved, and the market demand of healthy meal replacement food is met.

The invention belongs to the technical field of natural textile material modification, and particularly relates to a preparation method of bio-enzyme synergistic microorganism modified high-toughness mudar floss, which comprises the following steps: taking mudar floss as a raw material matrix, specifically providing clean mudar floss, and performing opening and activating treatment; performing solid-state fermentation treatment on the mudar floss by using brown rot fungi, specifically, inoculating activated brown rot fungi into the mudar floss for solid-state fermentation, and selectively degrading non-lignin impurities such as hemicellulose and the like; according to the preparation method of the high-toughness calotropis gigantea fiber modified by the bio-enzyme and the microorganisms, a biological method is adopted in the whole process, environmental pollution and fiber damage caused by traditional chemical modification are avoided, the process is mild and clean, a three-step synergistic strategy of brown rot fungus pretreatment, compound enzyme fine trimming and tannic acid toughening is innovatively adopted, and the method is suitable for industrial production. The brown rot fungi create a channel for subsequent enzyme reaction, enzyme treatment exposes active sites for tannic acid cross-linking, progressive layer by layer, high efficiency and remarkable effect are achieved.

The invention provides a complex microbial inoculant for fermenting modified corn flour, a method and application, and belongs to the technical field of fermented food. The complex microbial inoculant comprises a liquid state fermentation complex microbial inoculant (streptococcus thermophilus is greater than or equal to 1 * 10 < 9 > cfu / mL and bacillus subtilis is greater than or equal to 1 * 10 < 9 > cfu / mL) and a solid state fermentation complex microbial inoculant (monascus spore suspension is greater than or equal to 1 * 10 < 6 > spores / mL and lactobacillus plantarum is greater than or equal to 1 * 10 < 8 > cfu / mL), and the fermentation modification method comprises the following steps: performing microwave puffing treatment on corn flour, mixing the corn flour with oat bran, and sequentially performing liquid state fermentation and solid state fermentation, and spraying the liquid fermentation supernatant back to the solid substrate, and finally drying to obtain the modified corn flour. Through the synergistic effect of a two-stage fermentation system, the content of functional components such as soluble dietary fibers and Monacolin K in the corn flour is remarkably increased, and the obtained product has the characteristic of lowering cholesterol.

The invention discloses a method for increasing the content of total saponins in polygonatum kingianum through solid-state fermentation of aspergillus niger. According to the method, the polygonatum kingianum is subjected to solid-state fermentation by utilizing the aspergillus niger, the content of total saponins in the polygonatum kingianum after solid-state fermentation is 2-3 times that of the total saponins in unfermented polygonatum kingianum, and high-quality raw materials and technical supports are provided for increasing the additional value of polygonatum kingianum resources and developing functional products. According to the method disclosed by the invention, the polygonatum kingianum original product is fermented, so that the content of active ingredients of polygonatum kingianum is comprehensively increased, redundant wastes are not generated, the waste of resources is not caused, and an idea is broadened for developing high-added-value healthy foods and other medicinal products.

The invention relates to the technical field of microorganisms, and particularly discloses a metagen as well as a preparation method and anti-inflammatory application thereof. The method for preparing the metagen comprises the following steps: (1) carrying out solid-state fermentation by taking bacillus amyloliquefaciens CAU X1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.26769 and bacillus coagulans B2 with the preservation number of CGMCC No.26767 as zymophyte together, so as to obtain a fermentation product, wherein the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together, and the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together; (2) extracting the fermentation product by using a water-soluble solvent, centrifuging to take supernatant, and freeze-drying to obtain powder; or extracting the fermentation product by using an organic solvent, centrifuging and taking the supernatant, and volatilizing the organic solvent to obtain the extract. The metagen provided by the invention has anti-inflammatory activity, can improve bacterial enteritis, can play roles in resisting inflammation, regulating body immunity, optimizing intestinal flora structure and improving bacterial enteritis, can be used for medicines or feed additives, and has a good application prospect.

The invention provides an agricultural waste composite modified edible fungus culture medium and a preparation method thereof. The agricultural waste composite modified edible fungus culture medium is prepared from the following components in parts by mass: 20 to 30 parts of corn straw, 15 to 25 parts of rice hull, 10 to 20 parts of sawdust, 25 to 35 parts of cottonseed hull, 5 to 10 parts of soybean meal, 0.5 to 2.0 parts of complex microbial inoculant, 3 to 8 parts of bentonite, 1 to 3 parts of chitosan, 1 to 2 parts of gypsum and 1 to 2 parts of lime. According to the invention, the complex microbial inoculants are adopted to carry out solid state fermentation modification on the basic mixture, bentonite and chitosan are compounded into the activated functional slurry, and through the synergistic effect of solid state fermentation and the functional slurry, the granular structure, water retention and air permeability of the matrix are fundamentally improved, and efficient activation and slow release of nutrients are realized; finally, the fruiting period of the shiitake mushrooms is shortened to be within 85 days, the yield is increased by 10% or above, the shiitake mushroom proportion is remarkably increased, and high-value utilization of agricultural waste is achieved.

The invention relates to the technical field of fermentation, and discloses a meat solid-state fermentation device and method.The meat solid-state fermentation device comprises a fermentation device box, a driving motor, a lifting detection mechanism, a fermentation progress detection cleaning mechanism and a fermentation progress detection opening opening and closing mechanism, a feeding opening is formed in the position, close to the left end, of the top of the fermentation device box, and an upper cover plate is arranged above the feeding opening; a rear plate is mounted at the rear end of the fermentation device box; a turning structure is arranged in the middle of the fermentation device box; a detection port is formed near the right end of the top of the fermentation device box, and the fermentation progress detection port opening and closing mechanism is arranged at the detection port; the lifting detection mechanism is arranged at the left end above the detection port, the fermentation progress detection cleaning mechanism is arranged at the right end above the detection port, the fermentation progress detection cleaning mechanism comprises a detection cleaning adding structure and a detection cleaning supporting structure, and the detection cleaning supporting structure is arranged at the right end of the detection cleaning adding structure. According to the invention, full mixing is realized, the situation of insufficient local fermentation is avoided, and the fermentation quality and efficiency are improved.

The invention discloses a food for controlling postprandial blood sugar based on compound fermentation of plant raw materials and a preparation method of the food, and relates to the technical field of fermentation processing of functional foods and natural products. The sugar-controlling food contains plant raw material composite ferment powder, and a preparation method of the ferment powder comprises the following steps: S1, mixing mulberry leaf powder, buckwheat powder and soybean powder in proportion, and adding water to prepare a mixture with the water content of 55-60%; s2, inoculating a sugar-inhibiting eurotium cristatum liquid into the mixture, and performing solid-state fermentation to obtain a first fermented material; s3, inoculating the first fermented material with a lactobacillus plantarum bacterial liquid for continuous fermentation to obtain a second fermented material; and S4, carrying out enzyme deactivation, cooling, homogenizing, drying and crushing on the second fermentation material to obtain the plant raw material composite fermentation powder. The fermentation method disclosed by the invention not only can improve the content of hypoglycemic active ingredients and the inhibition rate of alpha-glucosidase, but also can reduce the bitterness of the active ingredients and balance the mouth feel.

The invention discloses a Chinese herbal medicine leavening, a preparation method thereof and application of the Chinese herbal medicine leavening in cosmetics, and particularly relates to the technical field of cosmetics. Comprising the following raw materials in parts by weight: 12-18 parts of radix astragali, 8-12 parts of radix ampelopsis, 10-15 parts of radix saposhnikoviae, 18-22 parts of rhizoma chuanxiong, 6-9 parts of bitter almond seeds, 18-22 parts of radix salviae miltiorrhizae, 7-10 parts of herba centellae, 6-9 parts of folium artemisiae argyi, 7-10 parts of fructus gardeniae, 4-6 parts of flos sophorae immaturus, 5-8 parts of fructus citri, 12-15 parts of poria cocos and 1.5-2.5 parts of a dendrobium officinale extract. The composition can synergistically exert moisturizing, immune regulation and anti-allergy effects with other raw material components to form an anti-allergy, anti-oxidation and repair efficacy closed loop, a process chain from aspergillus oryzae high-enzyme-activity solid fermentation to double-bacterium liquid symbiosis to chitinase directional enzymolysis can synergistically improve the yield of active matters, and the situation that traditional anti-allergy components have no hair growth effect is broken through, so that the anti-allergy and anti-allergy effects are achieved. Due to the limitation that the hair restorer stimulates sensitive skin, the hair growing and double effects are integrated, and the hair restorer is suitable for preparing cosmetics for resisting allergy, resisting oxidation and repairing barriers and is especially suitable for sensitive skin care and scalp care.

The invention relates to the field of food processing, and particularly discloses composite yeast for low-GI bread, a preparation method of the composite yeast and the low-GI bread. The low-GI bread composite yeast comprises the following raw materials in parts by weight: 4-5 parts of saccharomyces cerevisiae, 3-5 parts of lactobacillus plantarum, 1-2.5 parts of pediococcus pentosaceus, 100-150 parts of solid state fermentation pomace powder, 0.1-0.5 part of an enzyme inhibitor and 25-40 parts of millet resistant starch. The compound yeast is used for preparing the bread with the low GI value, and the prepared bread is low in glycemic index, good in expansibility, large in size, good in water binding capacity and not prone to hardening.

The invention relates to the technical field of microorganisms, and discloses a plant lactobacillus capable of inhibiting growth of monascus and application of the plant lactobacillus. The preservation number of the plant lactobacillus is CGMCC (China General Microbiological Culture Collection Center) No.34851. According to the present invention, the plant lactobacillus CCNH295 has strong bacterial inhibition ability on common soybean meal contaminating molds, such as aspergillus flavus, aspergillus ochraceus, fusarium moniliforme and penicillium; the plant lactobacillus CCNH295 has excellent bacteriostatic ability to monascus, and when the plant lactobacillus CCNH295 is used for fermentation production of soybean meal, growth of monascus can be remarkably inhibited, so that mildewing of the fermented soybean meal is effectively inhibited, and the prepared fermented soybean meal does not mildew after being placed at 37 DEG C for 6 months; besides, the fermented soybean meal produced by solid state fermentation of the plant lactobacillus CCNH295 is used as a feed raw material, so that the stability of the feed can be improved, and the palatability of the feed can be improved.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.

The invention provides lactobacillus reuteri GY-18 and application thereof in increasing the content of astragaloside, and belongs to the technical field of microorganisms, fermentation and natural product biotransformation. The lactobacillus reuteri GY-18 capable of improving the content of astragaloside in a fermented astragalus membranaceus product is obtained through screening, the lactobacillus reuteri GY-18 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC M 20252796, and the lactobacillus reuteri GY-18 can be used for improving the content of astragaloside in the fermented astragalus membranaceus product. The lactobacillus reuteri GY-18 can secrete acetylxylan esterase for promoting conversion of astragaloside into astragaloside IV, has efficient specificity on conversion of astragaloside into astragaloside IV, and is high in conversion capacity; according to the method, the content of astragaloside IV is remarkably increased within 24 hours in a semi-solid state fermentation mode, and the method has good practicability.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes OsiLf-2, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The solid-state fermentation process disclosed by the invention can produce spores on a large scale, shortens the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; the obtained spores can be freeze-dried under the protection of a skimmed milk powder solution to form spore powder, so that the freeze-drying survival rate and the long-term storage stability of the spores can be improved. The spore provided by the invention can be used for promoting crop growth, is more environment-friendly to ecology especially when being used for preparing a microbial fertilizer, does not influence the agricultural ecological environment and human body health, can promote the growth and development of host plants, and is high in use value and good in application prospect.

The invention relates to the technical field of resources and environment, in particular to a method for pretreating lignocellulose by using a rumen microorganism dry method. The method comprises the following steps: crushing lignocellulose to 1-5cm, and filling the crushed lignocellulose into a sealed space; spraying microorganisms under an anaerobic condition according to a mass ratio of rumen microorganisms to lignocellulose volatile components of (0.015-0.03): 1, and controlling the water content of the system to be 30-60%; the pretreatment temperature is regulated and controlled to be 35-42 DEG C, the pH is 6.0-7.5, the pretreatment time is 10-14 days, so that the total abundance of the functional microorganisms Pseudocidovorax, Hallella and Prevotella is greater than or equal to 55%, and the three types of microorganisms respectively form strong interaction with specific companion bacteria; when the relative crystallinity of the lignocellulose is reduced by more than 15% and the volatile component is reduced by 10%, finishing the pretreatment; and performing process regulation and control according to the crystallinity and the reducing sugar yield when the pretreatment is performed for 10 days. The dry process is adopted, the bionic principle and the solid-state fermentation technology are combined, all-component gradient utilization of lignocellulose is achieved, a green solution is provided for industrial and high-value conversion of lignocellulose, and remarkable economic, social and environment-friendly benefits are achieved.

The invention relates to the technical field of biology, in particular to a complex microbial inoculant and a method for improving quality and enhancing aroma of flue-cured tobacco by using the complex microbial inoculant, the tobacco is inoculated with the microbial inoculant before and after curing or in a re-drying link, and short-period solid state fermentation is carried out under controllable temperature and humidity conditions, so that the generation and conversion of aroma precursors in the tobacco are promoted, and the quality and aroma of the flue-cured tobacco are improved. The method has the advantages that pleasant aroma substances such as flower fragrance and fruit fragrance are directionally improved, fragrance fineness and harmony are improved, stimulation and offensive odor are reduced, and accordingly, stable improvement of flue-cured tobacco raw material flavor level and high-end formula adaptability is realized on the premise of not depending on a large amount of additional flavors and fragrances and being compatible with an existing processing technology.

The invention belongs to the technical field of food processing, and discloses a preparation method of fragrant nutritional peanut oil, which is based on a second-order squeezing process, and specifically realizes preparation of high-nutrient fragrant peanut oil through the working procedures of raw material soaking, irradiation germination, drying, crushing, pre-squeezing, puffing, solid state fermentation, steaming and frying, squeezing and the like. The obtained peanut oil has the characteristics of high extraction yield, rich nutrient substances, strong flavor, low content of harmful substances such as aflatoxin and benzopyrene, and the like.

The present application belongs to the technical field of microbial fermentation, and particularly relates to a strain of Kroppenstedtii ivory color and application thereof. In order to provide more high-temperature-resistant and pyrazine-producing brewing microbial resources, a new strain of Kroppenstedtii ivory color MZ35 is isolated from Maotai-flavor Daqu, and the preservation number is CCTCC NO: M 20252598. The strain can adapt to the solid-state fermentation environment of higher temperature and the pile fermentation environment of Maotai-flavor liquor, and promote the formation of tetramethylpyrazine. After MZ35 is inoculated into the solid-state fermentation system of highland barley or the intensified fermentation system of wine lees, the content of tetramethylpyrazine can be significantly increased. In addition, the strain can also promote the accumulation of acetoin, trimethylpyrazine and other Maotai-flavor related substances or precursor substances. Therefore, the strain has important application value for the pile fermentation of liquor, the flavor regulation of wine lees and the preparation of solid-state fermentation products rich in tetramethylpyrazine.

The invention discloses a rapid monitoring system for the total phenol content of a solid-state fermentation product and application, the change of the total phenol content of a fermentation product in the solid-state fermentation process can be rapidly monitored, a solid-state fermentation total phenol content prediction model of ResNet50-LSTM is constructed, image data in the solid-state fermentation process is collected, and the total phenol content is predicted in combination with a deep learning method. Theoretical and software support is provided for intelligent monitoring of the solid state fermentation process. Meanwhile, the invention designs an image acquisition device which is convenient to disassemble and firm in positioning, so that a camera can be fixed on a window to more clearly acquire image data of the leavening in the tank body. Through the application of the multi-modal prediction model and the image acquisition device, clear and reliable fermentation product image information can be quickly acquired and transmitted to the connected control device for analysis, so that the prediction of the total phenol content of the solid fermentation product is realized.

This invention discloses a fermentation process and application of wheat germ, belonging to the field of feed additive technology. The fermentation process includes the following steps: mixing Bacillus coagulans powder (accession number CGMCC No. 1.17145), Lactobacillus salivarius powder (accession number CCTCC NO: M 2022848), and steam-sterilized and cooled wheat germ; performing solid-state fermentation in a sealed environment; and then sterilizing with ozone after fermentation to obtain fermented wheat germ. This invention produces highly nutritious fermented wheat germ through simple steps. This fermented wheat germ can be used as a feed additive for laying hens, improving their production performance.

The invention relates to the technical field of solid state fermentation, and discloses fermentation equipment which comprises a box body, partition plates are transversely arranged in the box body and divide the interior of the box body into a plurality of fermentation layers, and a left side plate and a right side plate of each fermentation layer are each provided with an air inlet connector and an air outlet connector; the fermentation bags are arranged in the fermentation layers, air inlets and air outlets are formed in the fermentation bags, the air inlets are connected with the inner ends of the air inlet connectors of the corresponding fermentation layers, and the air outlets are connected with the inner ends of the air outlet connectors of the corresponding fermentation layers; a first cavity is formed in the ventilation component, the first cavity is communicated with the outer ends of all the air inlet connectors, and an air source connector communicated with the first cavity is formed in the ventilation component. The invention also provides a fermentation system. The fermentation equipment disclosed by the invention has the functions of aerobic fermentation and anaerobic fermentation, can conveniently switch between the aerobic fermentation and the anaerobic fermentation, and avoids the problems of poor air permeability and overlarge pressure in the fermentation bag.

Provided are an Aspergillus niger, a microbial agent thereof, a solid-state fermentation broth thereof, a preparation method therefor, and a use thereof. The Aspergillus niger is Aspergillus niger AGM01, which is preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: M2024768. The Aspergillus niger has a relatively high enzyme activity of β-glucosidase. By performing fermentation and cultivation on the Aspergillus niger in a solid-state fermentation medium containing a small-molecule inducer so as to obtain a solid-state fermentation broth of the Aspergillus niger, the enzyme activity of β-glucosidase in the solid-state fermentation broth of the Aspergillus niger is improved.

The invention relates to the field of environmental pollution treatment and microorganisms, and discloses a preparation method of a trichoderma asperellum straw fermentation extracting solution and application of the trichoderma asperellum straw fermentation extracting solution in oxytetracycline pollution remediation or crop growth promotion. The method comprises the following steps: 1) performing solid-state fermentation on a trichoderma asperellum T1 seed solution to obtain a fermentation product; and 2) drying the fermentation product, adding the dried fermentation product into the leaching solution for leaching, and filtering to obtain the trichoderma asperellum straw fermentation extracting solution rich in trichoderma asperellum spores, enzyme and reducing sugar. The invention finds that the trichoderma asperellum T-1 has new application of repairing oxytetracycline pollution and promoting crop growth, can efficiently degrade oxytetracycline at normal temperature especially in the aspect of repairing oxytetracycline pollution, and has practical application value. Furthermore, compared with a non-extracted trichoderma asperellum leavening and pure trichoderma spores, the trichoderma asperellum straw fermentation extracting solution obtained by a specific process has a better oxytetracycline pollution repairing effect and a better crop growth promoting effect respectively in comparison with the non-extracted trichoderma asperellum leavening and the pure trichoderma spores.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

The invention relates to the related technical field of feed industry and livestock breeding, and discloses a combined solid-state fermentation device for solid-state fermentation of biogen, which comprises a combined fermentation tank, the combined fermentation tank comprises a first fermentation tank and a second fermentation tank, the first fermentation tank is internally provided with an inner tank body, and the second fermentation tank is internally provided with an outer tank body; a stirring blade device, a sweeping and crushing device and an inflating and extruding assembly are arranged in the inner tank body, a driving motor assembly is fixedly mounted above the stirring blade device, and the driving motor assembly provides rotating power for the stirring blade device and the sweeping and crushing device; according to the invention, the improved design that the three-dimensional compound motion of rotation and up-down reciprocating is realized by matching with the bidirectional threaded rod, the X-shaped blade layout, the inclined flow guide groove and the rough rough surface convex small points in the groove are adopted, and the synchronous motion is realized by sharing a power source with the driving motor assembly and the cleaning and crushing device is adopted, so that local fermentation dead angles of the traditional equipment are thoroughly eliminated; the product quality difference caused by non-uniform mixing is avoided.

The invention relates to the technical field of biological waste treatment, in particular to a preparation method and application of a silkworm sand feed additive. The invention provides a technical scheme for preparing a silkworm and mulberry sand feed additive rich in acid protease, pectinase and cellulase by taking silkworm sand as a core matrix and performing directional pretreatment, adaptive strain fermentation and standardized post-treatment. According to the invention, silkworm sand-corncob powder is used as a composite substrate, and the silkworm sand and the corncob powder complement each other in function, so that the problems of unbalanced nutrition and poor air permeability of a single substrate are solved. Specific aspergillus niger is used for segmented solid-state fermentation, parameters are set in a differentiated mode, and full-amount utilization of the silkworm sand is achieved; the product quality and enzyme activity are improved by optimizing the process, and the resource value is improved from the low-added-value field to the high-added-value enzyme preparation industry.

The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method of fermented pinellia tuber, which comprises the following specific steps: S1, crushing rhizoma pinelliae preparata and medicated leaven into fine powder; s2, preparing ginger juice; s3, dissolving alum in a proper amount of water, adding the ginger juice, uniformly mixing, and uniformly mixing with the rhizoma pinelliae preparata, the medicated leaven, the flour and the raw bran which are crushed into fine powder; s4, knocking out an ochratoxin synthetic gene (otaA) from the aspergillus oryzae CICC 40186 through CRISPR-Cas9, and transferring the ochratoxin synthetic gene (otaA) into a lactic dehydrogenase gene (ldhL) of lactobacillus plantarum; s5, preparing a compound bacterium suspension; s6, placing the inoculated material in a solid state fermentation reactor; s7, vacuum freeze drying and variable-temperature drying are combined; the consistency of components of different batches of fermented pinellia can be guaranteed, the risk of infectious microbe contamination can be avoided, ochratoxin can be completely eradicated, the fermentation period can be greatly shortened, the overall production efficiency can be improved, the content of the key active component gamma-aminobutyric acid can be greatly improved, and the stability between batches is good.