636 results about "Solid-state fermentation" patented technology

The invention provides velvet antler mushroom sporocarp water extraction residue polysaccharide as well as an extraction method and application thereof, and belongs to the technical field of edible mushroom polysaccharide preparation. The invention provides an extraction method of water extraction residue polysaccharide of a mushroom fruiting body, which comprises the following steps: sequentially carrying out solid state fermentation, post-treatment and hot water extraction on the water extraction residue of the mushroom fruiting body to obtain an extraction product containing the water extraction residue polysaccharide of the mushroom fruiting body. According to the method disclosed by the invention, the water extraction residues of the pilose antler mushroom sporocarp are used as raw materials, so that high-value utilization of the water extraction residues of the pilose antler mushroom sporocarp can be realized. Moreover, the method can improve the yield and content of polysaccharide, and has the advantages of no chemical residue, ecological environmental protection, high production efficiency and the like. Besides, the polysaccharide prepared by the extraction method disclosed by the invention is stable under a digestion condition, and the prepared polysaccharide can improve the content of short-chain fatty acids in intestinal tracts, so that a basis is provided for application of the polysaccharide of the water-extracted residues of the fruiting bodies of the velvet antler mushrooms in new potential prebiotics.

The invention discloses saccharomyces cerevisiae FNFHSc18 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain FNFHSc18 for synthesizing immune nutrients-beta-carotene and astaxanthin, and the preservation number of the saccharomyces cerevisiae engineering strain is CGMCC (China General Microbiological Culture Collection Center) No.24838; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid state fermentation substrate, the content of crude protein and amino acid of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene and astaxanthin, and the content of the beta-carotene and the astaxanthin is greatly reduced. The soybean meal protein obtains new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and the soybean meal reaches a leading level in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.

The invention discloses a composition with high ultraviolet damage resistance as well as a preparation method and application thereof, and particularly belongs to the technical field of skin care product raw materials. The composition with high ultraviolet damage resistance is prepared by performing solid-state fermentation on radix scutellariae roots, radix ligustici sinensis and radix puerariae through compound bacteria consisting of bacillus subtilis, lactobacillus bulgaricus and bacillus coagulans and then performing solvent extraction. The composition with high ultraviolet damage resistance has excellent effects of resisting oxidative damage and inhibiting inflammatory factors, and can achieve the effects of repairing skin barriers, resisting inflammation, relieving, efficiently promoting repair of after-sun skin and the like.

The invention discloses saccharomyces cerevisiae MBPSc21 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc21 for expressing immune nutrient-beta-carotene, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc21 is CGMCC No.32663; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene, and the content of the beta-carotene is reduced. The soybean meal protein is endowed with new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and a leading level is achieved in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention discloses a fermentation preparation process for reducing cold and cool characteristics of fructus forsythiae tea. The fermentation preparation process comprises the following steps: performing early-stage treatment of screening, weighing, cleaning, high-temperature softening, rolling and high-temperature steam sterilization on fructus forsythiae leaves; compounding lactobacillus plantarum and German-style lactobacillus bulgaricus to obtain a mixed strain, adding the mixed strain into the treated fructus forsythiae leaves, and putting into a fermentation box for solid-state fermentation; under a sterile condition, adding a fermentation strain of pichia guilliermondii BC 621 into the fructus forsythiae leaves subjected to solid-state fermentation, and putting into a fermentation box for secondary fermentation; and drying the fructus forsythiae leaves subjected to secondary fermentation until the water content is below 6%. Active components reflecting cold and cool characteristics in the fructus forsythiae tea prepared by the method are obviously reduced, the fructus forsythiae tea is also suitable for people with deficiency-cold spleen and stomach and deficiency-cold constitution to drink, and audiences are expanded.

The invention belongs to the technical field of genetic engineering, and particularly relates to a high-chitin-content engineering bacterium as well as a construction method and application thereof. According to the invention, enzyme related to filamentous fungus chitin synthesis is subjected to overexpression through a genetic engineering means to construct engineering bacteria, the obtained engineering bacteria have high chitin content, and a high-strength mycelium material can be obtained through conventional solid state fermentation. The invention provides a feasible solution with controllable product quality for the production of the high-strength mycelium material, and has good application value.

The invention relates to the technical field of medicine preparation, in particular to a preparation method of licoflavone. The preparation method comprises the following steps: providing a licorice raw material, crushing, sieving, and carrying out solid-state fermentation to obtain licorice powder after solid-state fermentation; the preparation method comprises the following steps: adding a first mixed organic solution into a reaction kettle, soaking, adding a second mixed organic solution into the first mixed aqueous solution again, soaking, adding a second mixed organic solution, extracting, collecting an organic phase, carrying out enzymolysis enzyme deactivation and impurity removal on a solid product, and adding the second mixed organic solution again for extracting; and combining the organic phases, dialyzing, purifying and concentrating to obtain the licoflavone.

The invention discloses bacillus subtilis and application thereof. The bacillus subtilis LDH-Y3 is obtained by being separated from Guangzhou commercially available Rakang goat milk pasteurized milk, and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 64442. The bacterial strain is high in growth speed and high in activity, the maximum viable count reaches 9.36 log CFU / mL, and the bacterial strain has good gastrointestinal tract digestion resistance. The bacillus subtilis is high in cellulase production capacity, CMCase (Carboxymethyl Cellulase) reaches 39.66 U / mL, beta-Gase (beta-Gase) reaches 2.36 U / mL, and Fpase (Fpasease) reaches 11.37 U / mL. The strain has the capability of inhibiting helminthosporium convex hilum and helminthosporium longibrachiatum, and after solid state fermentation of corn straw, the content of neutral detergent fibers in the corn straw can be reduced, and the digestibility of the straw is improved.

The invention discloses a traditional Chinese medicine residue organic compound fertilizer special for acid-base imbalance barren soil and a production method, and relates to the technical field of soil improvement and fertilizer.The organic compound fertilizer is prepared from, by volume, 50-70 parts of fermented traditional Chinese medicine residue matrix, 15-30 parts of mineral nutrient unit, 5-15 parts of biological activity enhancer and 3-10 parts of structure conditioner; wherein the fermented traditional Chinese medicine residue matrix is prepared by taking traditional Chinese medicine pharmacy mixed residues as raw materials and performing solid-state fermentation through a complex microbial inoculant containing aspergillus niger and the like; the mineral nutrient unit is compounded by urea and the like; the biological activity enhancer contains humic acid and the like; the structure conditioner is a mixture of attapulgite and sodium carboxymethyl cellulose (5: 1-8: 1). According to the organic compound fertilizer, the fermented traditional Chinese medicine residues with the organic matter content larger than or equal to 45% serve as a matrix, the mineral nutrient unit, the biological activity enhancer and the structural conditioner which are coated in a slow-release mode are matched, a synergistic system is formed, three-stage fermentation is adopted, the microbial activity is kept, and the pH lt can be improved; 6.0 or gt; 8.5 of sand / clayey barren soil.

The invention discloses a method for producing pectinase by using trachycarpus racemosus based on a single factor test and application, and the method comprises the following steps: selecting a fermentation strain, setting a solid state fermentation culture medium, setting fermentation culture conditions, inoculating the fermentation strain into the solid state fermentation culture medium, testing the performance of pectinase, and determining the content of pectinase according to the test result. And optimizing the solid-state fermentation culture medium and the fermentation culture condition to obtain the optimal solid-state fermentation culture medium setting condition and the optimal fermentation culture condition. Compared with the prior art, the method has the advantages that the quantity and vitality of spores are remarkably improved by selecting the trachycarpus racemosus strains as the fermentation strains, so that the vitality and production efficiency of the strains to be fermented are improved, and the optimal solid-state fermentation culture medium setting conditions and the optimal fermentation culture conditions are obtained by optimizing the solid-state fermentation culture medium setting conditions and the fermentation culture conditions; the optimized pectinase product is obtained under the optimized condition, and the pectinase product has high activity and excellent high temperature resistance and acid resistance, so that the application range of pectinase is widened.

The invention discloses a Coaerella coagulum-plant lactobacillus synergistic fermentation inoculant and a process for staged solid-state fermentation of American ginseng by using the same, and belongs to the technical field of biological fermentation. According to the invention, two strains, i.e., the plant lactobacillus and the hainerella coagulatum, are obtained by screening food-grade probiotic strains, and the ginsenoside conversion capability of the two strains is verified by utilizing culture. The invention also provides a probiotic fermentation process for improving the content of rare ginsenoside in American ginseng, namely, the staged synergistic fermentation of the Halidernia coagulum and the phytobacterium plantarum is utilized, so that the content of the rare ginsenoside in the American ginseng is improved; cellulase and hemicellulase secreted by the Haihdrikia coagulatum can decompose cellulose, lignin and the like in cell walls of the surface layer of the American ginseng raw material in the early stage of fermentation, so that ginsenoside in cells is fully released, and beta-glucosidase from different sources is utilized to synergistically convert prototype ginsenoside in the later stage of fermentation. The content of various rare ginsenosides in American ginseng can be remarkably increased through the solid-state fermentation process. Meanwhile, the solid-state fermentation process has the advantages of mild conditions, less wastewater treatment, environmental protection, low cost, process safety and the like, has important significance in improving the resource utilization of American ginseng and promoting the industrial development of American ginseng, and has relatively good practicability.

The invention discloses a making method and application of saccharopolyspora baking koji and malt koji for brewing food, and belongs to the field of microbial technology and brewing production thereof. The preparation method comprises the following steps: by taking saccharopolyspora roseus preserved in a laboratory as a function strengthening strain and taking crushed red wheat as a carrier, adding baked wheat or wheat malt, proportionally inoculating a saccharopolyspora roseus solution, uniformly stirring, and culturing in a solid-state fermentation box for 120 hours to prepare the strengthened saccharopolyspora roseus wheat koji. The saccharifying power of the finally obtained wheat koji can reach 2769.46 + / -22.37 U / g absolute dry wheat koji, the liquefying power of the finally obtained wheat koji can reach 2.36 + / -0.11 U / g absolute dry wheat koji, the saccharifying power and the liquefying power of the finally obtained wheat koji are both higher than those of original raw wheat koji, the stability of the wheat koji is further improved, and the content of flavor substances including nitrogen-containing flavor compounds, aldehyde ketone substances and ester substances in the yellow wine is increased.

The invention discloses a fermented traditional Chinese medicine feed additive as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese veterinary medicines. The additive is prepared by solid state fermentation and extraction of a traditional Chinese medicine composition and a complex microbial inoculant, the traditional Chinese medicine composition comprises poria cocos, astragalus membranaceus, codonopsis pilosula, pericarpium citri reticulatae and radix puerariae, and the complex microbial inoculant comprises bacillus subtilis, lactobacillus plantarum and aspergillus niger. Through a fermentation and extraction combined process, the effective components in the traditional Chinese medicines are higher in extraction rate and easier to absorb, the animal intestinal flora balance can be remarkably regulated, the immunity is enhanced, the growth performance is promoted, and the prospect is wide.

The invention discloses an aspergillus cristatus strain capable of increasing gastrodin content and application of the aspergillus cristatus strain, and belongs to the technical field of solid state fermentation engineering. The Aspergillus cristatus strain ASE-4 is preserved in the China Center for Type Culture Collection, and the preservation number of the Aspergillus cristatus strain ASE-4 is CCTCC (China Center for Type Culture Collection) NO: M20241392. By optimizing the fermentation method and modeling through the ANN-GA algorithm (R2 = 0.92), the gastrodin content (50% higher than that of an unfermented group) and the polysaccharide and total flavone content are remarkably improved. The strain and the fermentation method provided by the invention solve the problem that the gastrodin content is reduced in the prior art, and are suitable for development of high-added-value gastrodia elata products.

The invention discloses a mulberry leaf-vinasse fermentation composition which comprises the following raw materials: vinasse, mulberry leaves and a complex microbial inoculant, the complex microbial inoculants comprise saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum. The mulberry leaf-vinasse fermentation composition is obtained by performing anaerobic solid state fermentation on the raw materials, namely crushing vinasse and mulberry leaves to 20-50 meshes, uniformly mixing, adjusting the water content of the raw materials to be 50-70wt%, inoculating a complex microbial inoculant, uniformly stirring, filling into a breathing bag, sealing at 37 + / -0.5 DEG C, and fermenting for 4-6 days, thereby obtaining the mulberry leaf-vinasse fermentation composition. According to the mulberry leaf-vinasse fermentation composition, the mulberry leaves and the vinasse are mixed, then the complex microbial inoculant is inoculated for solid-state fermentation, the pH value of the prepared mulberry leaf-vinasse fermentation composition is remarkably reduced, meanwhile, the lactic acid content is remarkably increased, the antinutritional factor content is reduced, true protein is well preserved, the antioxidant activity is more reserved, and the fermentation effect of the mulberry leaves and the vinasse is improved; the feeding value of the mulberry leaves and the vinasse is effectively improved.

The invention belongs to the technical field of food flavor engineering, and discloses a Maotai-flavor Baijiu flavor composite essence based on a non-volatile substance release promoting effect, and the composite essence is composed of a volatile aroma substance, a non-volatile organic acid and an amino acid. Aiming at the technical bottlenecks of rapid attenuation of aroma intensity of volatile components and single flavor dimension in the existing Maotai-flavor Baijiu flavor compound essence blending technology, the invention creatively constructs a molecular synergistic system of volatile and non-volatile flavor components. By adding non-volatile substances, the effect of enhancing the flavor of the Maotai-flavor Baijiu flavor composite essence is achieved. The technology not only completely reproduces the flavor structure of the traditional solid-state fermented Maotai-flavor liquor, but also endows the compound liquor and flavor foods (such as Maotai-flavor baked products, compound seasonings and non-alcohol ready-to-drink beverages) with a continuous and stable aroma releasing characteristic, and provides a molecular-level regulation and control scheme for upgrading the sensory quality of liquor flavor standardization and food industrial production.

The invention relates to the technical field of functional food production through microbial fermentation, in particular to a herba epimedii enzymolysis probiotic fermentation refined extract and a preparation method thereof.The preparation method comprises the following steps that herba epimedii and beta-cyclodextrin are mixed, an obtained mixed solution is subjected to enzymolysis treatment through pectinase, cellulase and glycosidase and then serves as a solid-state fermentation substrate, and the herba epimedii enzymolysis probiotic fermentation refined extract is obtained; adding mixed bacteria of lactobacillus casei, bifidobacterium lactis and lactobacillus gasseri, performing solid-state fermentation, and performing vacuum freeze drying to obtain a herba epimedii probiotic fermentation refined extract; the herba epimedii probiotic fermentation refined extract is prepared by adopting an enzymolysis fermentation method in cooperation with a beta-cyclodextrin inclusion technology, the extraction efficiency, the solubility, the biological activity, the stability and other performance of effective components in herba epimedii are improved, the preparation method is simple, and operation is easy and convenient; the used materials are edible, green and safe; the process saves energy consumption, has good environmental benefits, greatly saves manpower and material resources, and can be applied to industrial large-scale production.

The invention discloses a method for producing nattokinase by solid-state fermentation of hemp seed meal. Hemp seed meal is used as the only matrix, nattokinase producing bacteria are inoculated on the solid culture medium, the temperature is controlled at 30-40°C, the humidity is controlled at 60-80°C, and the fermentation is carried out for 16-24 hours, and the enzyme production of nattokinase reaches 9592.8IU / g. The invention uses hemp seed meal, which is a waste of oil extraction, as the only fermentation matrix, greatly reduces the production cost of nattokinase, simplifies the fermentation process, and realizes the high-value utilization of hemp seed meal. The fresh fragrance of hemp seeds well neutralizes the ammonia smell generated during the fermentation process of nattokinase, making nattokinase more acceptable. The method is the first to use hemp seed meal solid-state fermentation to produce nattokinase, provides a new method for the production of nattokinase, and has broad application prospects.

The invention provides a fermentation method for producing single-cell protein from corn straw. The fermentation method comprises the following steps: (1) inoculating a microbial seed solution into a crushed corn straw solid culture medium; (2) after the solid-state fermentation is completed, adding water, and reacting at 65-85 DEG C for 4-8 hours; and (3) cooling the liquid culture medium, and then inoculating a microbial seed solution for fermentation. By adopting the method, the yield of single-cell protein can be up to 142.68 g / L, and the yield of crude protein can be up to 44.8%. Compared with direct fermentation of the corn straw, the yield of single-cell protein and the content of crude protein can be remarkably increased, and the fermentation method for achieving efficient utilization of the corn straw is provided.

The invention relates to the technical field of sewage biochemical treatment, and discloses a preparation method of an efficient composite biological carbon source, which comprises the following steps: solid-liquid two-phase fermentation treatment, microbial community echelon relay treatment, fermentation liquor reflux and biological detoxification system and biomass enhancement treatment. According to the invention, the preparation of the composite biological carbon source with high concentration (COD > = 50,000 mg / L), high stability (stable operation time > = 120 days) and high activity is realized by combining solid state fermentation and liquid state fermentation, constructing a microbial echelon metabolic network, applying a porous carrier layer to eliminate an inhibitor, implementing active factor regeneration and other technical means; according to the present invention, the organic matter conversion efficiency is improved by more than 30%, the stable operation time of the system is improved, the technical problems of insufficient concentration, unbalanced components, poor stability and the like in the traditional carbon source preparation are solved, the method is suitable for the nitrogen and phosphorus removal process of the large-scale sewage treatment plant, the total nitrogen removal rate of the sewage treatment system can be improved by 15-25%, and the nitrogen removal rate can be improved by 30-40%.

The utility model relates to the technical field of bioreactors, and discloses a semi-solid fermentation bioreactor, which comprises a tank body with a feed port and a discharge port, a rotary guide cylinder is arranged in the tank body, the rotary guide cylinder can rotate around the axis of the rotary guide cylinder, and the feed port is communicated with the discharge port. The inner wall and / or the outer wall of the rotary guide cylinder are / is provided with a spiral band extending along the length direction of the rotary guide cylinder, the rotary guide cylinder is arranged in the tank body, and the inner wall and / or the outer wall of the rotary guide cylinder are / is provided with the spiral band extending along the length direction of the rotary guide cylinder. The spiral belt can forcibly drive reaction materials in the tank body to ascend or descend when the rotary guide cylinder rotates around the axis of the rotary guide cylinder, so that the reaction materials in the tank body are fully mixed, and the purpose of strengthening mass transfer and heat transfer of semi-solid fermentation materials is achieved.

The invention provides high-satiety chicken small molecule peptide and application thereof in chicken meal replacement powder.The method comprises the steps that firstly, intragastric self-swelling microspheres are prepared, wall materials are yeast beta-glucan and hydroxypropyl methyl cellulose, a core material is modified chicken small molecule peptide, the microspheres absorb water to swell in a gastric acid environment to generate satiety, and the self-swelling microspheres absorb water to swell in the gastric acid environment; the modified chicken small molecule peptide is slowly released in an intestinal environment, so that absorption is promoted, and intestinal health is regulated; in addition, the coarse cereal powder is subjected to solid state fermentation by using aspergillus oryzae and then is used for preparing the chicken meal replacement powder, and the self-expanding microspheres in the stomach and the fermented coarse cereal powder are added, so that the satiety is improved, and the market demand of healthy meal replacement food is met.

The invention belongs to the technical field of natural textile material modification, and particularly relates to a preparation method of bio-enzyme synergistic microorganism modified high-toughness mudar floss, which comprises the following steps: taking mudar floss as a raw material matrix, specifically providing clean mudar floss, and performing opening and activating treatment; performing solid-state fermentation treatment on the mudar floss by using brown rot fungi, specifically, inoculating activated brown rot fungi into the mudar floss for solid-state fermentation, and selectively degrading non-lignin impurities such as hemicellulose and the like; according to the preparation method of the high-toughness calotropis gigantea fiber modified by the bio-enzyme and the microorganisms, a biological method is adopted in the whole process, environmental pollution and fiber damage caused by traditional chemical modification are avoided, the process is mild and clean, a three-step synergistic strategy of brown rot fungus pretreatment, compound enzyme fine trimming and tannic acid toughening is innovatively adopted, and the method is suitable for industrial production. The brown rot fungi create a channel for subsequent enzyme reaction, enzyme treatment exposes active sites for tannic acid cross-linking, progressive layer by layer, high efficiency and remarkable effect are achieved.

The invention provides a complex microbial inoculant for fermenting modified corn flour, a method and application, and belongs to the technical field of fermented food. The complex microbial inoculant comprises a liquid state fermentation complex microbial inoculant (streptococcus thermophilus is greater than or equal to 1 * 10 < 9 > cfu / mL and bacillus subtilis is greater than or equal to 1 * 10 < 9 > cfu / mL) and a solid state fermentation complex microbial inoculant (monascus spore suspension is greater than or equal to 1 * 10 < 6 > spores / mL and lactobacillus plantarum is greater than or equal to 1 * 10 < 8 > cfu / mL), and the fermentation modification method comprises the following steps: performing microwave puffing treatment on corn flour, mixing the corn flour with oat bran, and sequentially performing liquid state fermentation and solid state fermentation, and spraying the liquid fermentation supernatant back to the solid substrate, and finally drying to obtain the modified corn flour. Through the synergistic effect of a two-stage fermentation system, the content of functional components such as soluble dietary fibers and Monacolin K in the corn flour is remarkably increased, and the obtained product has the characteristic of lowering cholesterol.

The invention relates to the technical field of aquaculture, and discloses a preparation method of an aquatic fermented feed containing a composite probiotic inoculant, and the preparation method comprises the following steps: mixing bacillus amyloliquefaciens, streptococcus lactis and saccharomycetes in proportion to obtain the composite probiotic inoculant; the preparation method comprises the following steps: dissolving a compound probiotic agent and a fermentation aid in water, spraying the dissolved compound probiotic agent and the fermentation aid into the raw materials, adding a probiotic agent for mixing, quantitatively sub-packaging the mixed raw materials into breathing bags, and performing solid-state fermentation at the fermentation temperature of 25-40 DEG C for 3-7 days to obtain the aquatic product fermented feed. The method has the effect of improving the feed utilization rate.

The invention relates to the technical field of health-care food, in particular to a method for preparing selenium-enriched yeast. The preparation method comprises the following steps: S1, preparing mixed powder; s2, preparing a paste; s3, standing and fermenting the obtained paste; s4, adding anhydrous alcohol into the obtained fermentation product, stirring, centrifuging, taking precipitate, and recycling supernate; s5, adding purified water into the obtained precipitate, adding silicon dioxide at the same time, performing uniform stirring, and then uniformly laying the mixture on a fluidized bed distribution plate for drying to prepare a dry powder finished product; according to the present invention, the flour is adopted as the carbon source, the bean powder is adopted as the nitrogen source, the simplest solid state fermentation mode is adopted, the fermentation time is short, the selenium-rich yeast can be prepared in a large scale, and the carbon and nitrogen sources are the cheap edible raw materials, and no other chemical components are added, such that the complex separation purification process is not required after the fermentation is completed; the food can be directly eaten only through simple fluidized bed drying, and is low in cost, safe and reliable.

The invention discloses a solid-state fermented barley bran matrix characteristic detection method based on an RGB reconstructed hyperspectral image and application, 31 spectrum channels related to the water content and pH value of barley bran are screened out by using a competitive adaptive reweighting algorithm (CARS), and the RGB image of the fermented barley bran is collected to obtain the characteristic of the solid-state fermented barley bran matrix characteristic detection method based on the RGB reconstructed hyperspectral image. And an image processing technology and a deep learning algorithm are utilized to reconstruct a hyperspectral image, so that rapid and nondestructive detection of matrix characteristics such as moisture content and pH value in the solid-state fermented barley bran is realized. The method is an economical, simple, convenient and accurate solid state fermentation food agricultural product raw material nondestructive testing technology, is beneficial to development of a real-time and efficient solid state fermentation accurate control method, and is particularly suitable for being applied to the field of food quality, quality and safety control based on a solid state fermentation technology so as to optimize the fermentation process and improve the product quality; and the practical value is good.

The invention discloses a method for increasing the content of total saponins in polygonatum kingianum through solid-state fermentation of aspergillus niger. According to the method, the polygonatum kingianum is subjected to solid-state fermentation by utilizing the aspergillus niger, the content of total saponins in the polygonatum kingianum after solid-state fermentation is 2-3 times that of the total saponins in unfermented polygonatum kingianum, and high-quality raw materials and technical supports are provided for increasing the additional value of polygonatum kingianum resources and developing functional products. According to the method disclosed by the invention, the polygonatum kingianum original product is fermented, so that the content of active ingredients of polygonatum kingianum is comprehensively increased, redundant wastes are not generated, the waste of resources is not caused, and an idea is broadened for developing high-added-value healthy foods and other medicinal products.

The invention relates to the technical field of microorganisms, and particularly discloses a metagen as well as a preparation method and anti-inflammatory application thereof. The method for preparing the metagen comprises the following steps: (1) carrying out solid-state fermentation by taking bacillus amyloliquefaciens CAU X1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.26769 and bacillus coagulans B2 with the preservation number of CGMCC No.26767 as zymophyte together, so as to obtain a fermentation product, wherein the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together, and the bacillus amyloliquefaciens CAU X1 and the bacillus coagulans B2 are used as zymophyte together; (2) extracting the fermentation product by using a water-soluble solvent, centrifuging to take supernatant, and freeze-drying to obtain powder; or extracting the fermentation product by using an organic solvent, centrifuging and taking the supernatant, and volatilizing the organic solvent to obtain the extract. The metagen provided by the invention has anti-inflammatory activity, can improve bacterial enteritis, can play roles in resisting inflammation, regulating body immunity, optimizing intestinal flora structure and improving bacterial enteritis, can be used for medicines or feed additives, and has a good application prospect.