Nonreactive fermented feed of mixed bacteria and preparation method thereof

A technology of fermented feed and compound bacteria, applied in biochemical equipment and methods, animal feed, animal feed, etc., can solve the problems affecting the feeding effect of fermented feed, achieve the effect of strong cellulase production and inhibition of proliferation

Active Publication Date: 2012-06-20
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there are still some technical problems to be solved: the development of strains of fermented feed, the quality of

Method used

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  • Nonreactive fermented feed of mixed bacteria and preparation method thereof
  • Nonreactive fermented feed of mixed bacteria and preparation method thereof
  • Nonreactive fermented feed of mixed bacteria and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the preparation of composite bacteria liquid

[0029] (1) Preparation of various bacterial strains in the composite bacteria:

[0030] 1) Preparation of Bacillus subtilis CGMCC No.4628 bacterial liquid:

[0031] Preparation of Bacillus subtilis seed solution: Inoculate the strains preserved on the slant of the test tube into a 250ml Erlenmeyer flask containing 100ml of MNB medium, and culture in a constant temperature shaker at 30°C for 16h at a shaker speed of 225rpm.

[0032] Preparation of Bacillus subtilis bacterial liquid: Inoculate the prepared Bacillus subtilis seed liquid into a 250mL Erlenmeyer flask containing 100mL medium according to the inoculum amount of 1%, cultivate it in a constant temperature shaker at 30°C for 16h, and the shaker speed is 225 rpm.

[0033] The MNB medium of the present invention is: glucose, 5.0g / L; peptone, 5.0g / L; beef extract, 3.0g / L; yeast extract powder, 1.0g / L; magnesium sulfate heptahydrate, 0.5g / L ; Manganese ...

Embodiment 2

[0042] Embodiment 2, the steps of using compound bacteria to prepare non-antibiotic fermented feed and fermentation process parameters

[0043] (1) Preparation of the dry matrix of compound bacteria without antibiotic fermented feed: the dry matrix used to prepare compound bacteria without antibiotic fermented feed of the present invention is the part that compound feed does not comprise the premix of 4%, that is: first The part other than the 4% premix in the compound feed is mixed according to the ratio to make dry feed for fermentation. The compound feed formula is as follows (see Table 1):

[0044] Table 1. Formula of fermented feed compound feed

[0045]

[0046] (2) Preparation of composite bacteria liquid: three kinds of bacteria are taken from Example 1, the concentration of Bacillus subtilis is 4.20log CFU / mL; the concentration of Lactobacillus acidophilus is 4.12log CFU / mL; the concentration of Saccharomyces cerevisiae is 4.23log CFU / mL, according to the volume ...

Embodiment 3

[0056] Embodiment 3, the influence of composite bacteria solid-state fermentation of the present invention on feed microbial composition

[0057] (1) Collection of fermented feed samples prepared in Example 2: Samples were collected on the 0th (before fermentation), 1, 2, 3, 5, and 7 days after the start of fermentation, and analyzed for microbial composition. Detection indicators include: the number of Lactobacillus acidophilus, Bacillus subtilis, Saccharomyces cerevisiae and Escherichia coli.

[0058] (2) Microbiological determination of fermented feed samples: Microbiological determination adopts the 10-fold dilution method. Take 1 g of the sample and dissolve it in 9 mL of sterile water, then make a series of 10-fold dilutions, and select a suitable dilution gradient for determination.

[0059] Bacillus subtilis should be counted on the compound nutrient agar medium, and cultivated at 30°C for 16h.

[0060] For the determination of Lactobacillus acidophilus, MRS agar medi...

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Abstract

The invention relates to a nonreactive fermented feed of mixed bacteria and a preparation method thereof, which belong to the field of microbial fermented feed and solid feed. The nonreactive fermented feed is manufactured by mixing and fermenting solution of the mixed bacteria and dried matrix of compound feed and adding 4% of premix into the fermented solution after the fermentation. The fermented feed preparation technology is simple and convenient to operate and can be operated by various farmers and feed manufactures easily. The prepared solid feed is obvious in effect, capable of improving utilization of feed and promoting growth of animals, and capable of improving and maintaining balancing of intestinal flora, improving immunity and premonition of the animals, effectively improving body shape of pigs and increasing economic benefits. No antibiotic is added in the preparation process and finial products, the nonreactive fermented feed of the mixed bacteria and the preparation method thereof are healthy, safe and can be directly used in breeding of growing-finishing pigs.

Description

technical field [0001] The invention relates to a composite bacteria-free fermented feed and a preparation method thereof, belonging to the field of microbial fermented feed and solid feed. Background technique [0002] At present, with the improvement of people's living standards, food quality and safety issues are increasingly valued by the country and the people, and pork and its organs are the first to bear the brunt of the Chinese people's traditional non-staple food. However, the residues of antibiotics in pork and its organs have also produced direct or indirect harm to human health. Therefore, reducing or stopping the use of antibiotics in feed has become a task for scientific researchers to solve the quality and safety of pork. In recent years, the emergence of microecological preparations, enzyme preparations, Chinese herbal medicine preparations and organic acid antibiotic substitutes have played a great role in solving this problem, but these products still have...

Claims

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Application Information

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IPC IPC(8): A23K1/14C12N1/20C12N1/18C12R1/125C12R1/23C12R1/865
Inventor 胡婷彭子欣王安如刘滢刘婷
Owner BEIJING DABEINONG TECH GRP CO LTD
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