30results about How to "Good tolerance to bile salts" patented technology

The invention relates to a feed for early weaned piglets, which belongs to the field of the feed. Main ingredients of the feed comprise expanded corn, full-fat soybean, fat powder, steam fish meal, acidified whey powder, fermented soybean meal, a complex enzyme preparation, a micro-ecological preparation, amino acids, complex vitamins, complex trace elements and the like. The digestive physiological characteristics of the weaned piglets are fully considered on the selection and the processing of raw materials, thereby greatly improving the palatability and the digestion rate of the feed; simultaneously, through the synergistic compatibility of an egg yolk antibody, lactein, sodium humate and the complex micro-ecological preparation, the diarrhea rate of the weaned piglets is reduced, the effects are superior to those of adding antibiotics into the feed, and the feed is the green, environmentally-friendly and nuisanceless feed. An animal feeding experiment proves that the feeding effects are ideal, the palatability is good, the piglets after weaning are high in average feed intake, fast in daily weight gain and low in diarrhea rate, and the feed has a good promoting effect for growth and health conditions of the weaned piglets and can meet the demands of most of raisers at present.

Belonging to the premix field, the invention relates to an additive premix for laying hens. The premix of the invention mainly contains: soybean meal, calcium hydrogen phosphate, stone powder, salt, compound vitamin, nicotinic acid, D-calcium pantothenate, biotin, choline chloride, copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, potassium iodide premix, sodium selenite premix, ethoxy quinoline, phytase, microecologics, xylo oligosaccharide, bentonite and rice husk. With xylo oligosaccharide and a plurality of probiotics and without antibiotic, the premix in the invention hasa reasonable formula and high viable bacteria content. Animal feeding experiments prove that the additive premix in the invention has ideal feeding effects. The ammonia concentration, the death and culling rate, and the broken egg rate can be reduced, while the laying rate and egg weight are enhanced. The composite premix provided in the invention can well promote the laying performance and health status of laying hens, and meet the needs of mass raising households, and is suitable for green and healthy culture so as to produce pollution-free egg products.

The invention relates to compound probiotics and a preparation method thereof. The compound probiotics comprise ten or more probiotics and two or more prebiotics. The invention also provides the preparation method of the compound probiotics. The preparation method comprises the following steps: firstly, mixing the compound probiotics according to the proportion, passing through a sieve with 80 to 120 meshes, then taking siftage and weighing; secondly, mixing the weighed materials; thirdly, tabletting mixed materials. According to the compound probiotics and the preparation method thereof disclosed by the invention, by scientifically mixing the probiotics with the prebiotics, the efficacy of the compound probiotics is effectively improved, living cell concentration is high, and the efficacy realizing time of the probiotics is shortened; compared with other products in the market, the compound probiotics have the advantages that acid and bile salt resisting capabilities are good; if the compound probiotics are administrated for one month, the improvement rate of constipation reaches 100 percent, the improvement rate of chronic colitis reaches 100 percent, the positive-to-negative rate of helicobacter pylori reaches 82 percent, the rehabilitation rate of chemical liver injury reaches 88 percent, the rehabilitation rate of anaphylactic rhinitis is 91 percent, the rehabilitation rate of female vaginitis reaches 93 percent, the improvement rate of phlegm-dampness constitution reaches 100 percent, the improvement rate of damp-heat constitution reaches 100 percent, and the improvement rate of acnes reaches 98 percent.

The present invention discloses Lactobacillus paracasei ZLP019, which has a preservation number of CGMCC No.11532, and has characteristics of good acid resistance, good cholate resistance and good bacterial inhibition. According to the present invention, with the application of the Lactobacillus paracasei ZLP019 to prepare the freeze-drying preparation, the live bacteria survival rate is high, and the preparation is conveniently used in feed enterprises and farms; and after the preparation is directly added to sow feed according to the ratio requirement, the reproductive performance of sows can be improved, the daily weight gain and the survival rate of suckling piglets can be improved, the intestinal health of sows can be improved, the harmful bacteria emission in feces can be reduced, and the environmentally friendly breeding environment can be easily achieved.

The invention relates to a preparation technology of a bacillus feed additive agent, belonging to the field of feed additive agent. The preparation technology comprises the following steps: 1) culturing bacillus to obtain a seed liquid; 2) carrying out liquid fermentation culture of bacillus with the inoculation amount of the seed liquid is 3-10% and until the microbial content reaches 1-2*10<10>cfu/ml; 3) collecting thalli by centrifugation; 4) freeze-drying thalli: adding a freeze-drying protectant in the collected thalli, putting the mixture in a freeze dryer for vacuum freeze-drying to obtain freeze-dried bacterial powder; and 5) adding auxiliary materials in the freeze-dried bacterial powder to obtain the bacillus feed additive agent. The feed additive agent obtained by the preparation technology has good probiotic functions of stress resistance and preventing pathogenic bacteria such as gastric acid resistance, cholate resistance, high temperature resistance, antibiotic tolerance and the like, can be used in combination with antibiotics. The prepared feed additive has high viable bacteria content, long -term storage, and obvious and stable application effects.

The invention relates to a Bacillus coagulans-Lactobacillus casei-Lactobacillus plantarum probiotic inoculum and preparation thereof. The solid fermentation probiotic inoculum belongs to the technical field of feed microbes. The invention relates to an application technology which is implemented by carrying out solid state fermentation on bean pulp, bran and corn flour used as matrixes by using Bacillus coagulans HM-09, Bacillus licheniformis BL-09, Lactobacillus casei HM-09 and Lactobacillus plantarum HM-10, wherein the collection number of the Bacillus licheniformis BL-09 is CGMCC No.5686. The preparation method comprises the following steps: inoculating Bacillus coagulans, Lactobacillus casei and Lactobacillus plantarum into a solid culture medium, carrying out mixed fermentation for 18-28 hours, adding Bacillus licheniformis BL-09, and continuing fermentation for 20-28 hours to obtain the product. The product has special acid aroma, the viable count of the bean pulp-bran-corn flour fermented product is high, and the degradation rate of the bean pulp protein is high. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.

The invention discloses an application of lactobacillus fermentum CQPC04, the preservation number of which is CGMCC NO, 14493, in preparing food or drugs for improving ulcerative colitis. The application range of the lactobacillus fermentum CQPC04 is expanded, the development utilization value is improved, and a new hope is brought to improve the ulcerative colitis.

The present invention provides a lactobacillus paracasei subsp. paracasei. The lactobacillus paracasei subsp. paracasei is deposited in the China General Microbiological Culture Collection Center andhas deposit number of CGMCC NO.17893. The lactobacillus paracasei subsp. paracasei has certain antioxidant capacity, can remove DPPH and hydroxyl radicals, also has good salt resistance, acid resistance, bile salt resistance, gastric juice resistance, intestinal juice resistance, and hydrophobicity, thus increases chances of playing a probiotic role, and can be used in fields of fermented feeds, fermented food, health-care food, etc.

The invention relates to pure natural lactobacillus millet enzyme. The pure natural lactobacillus millet enzyme is prepared from the following steps: preparing powder: smashing millet, and screening by a 100-mesh sieve; carrying out saccharification liquid preparation: weighing 200g of obtained millet powder, adding 1000mL of water, heating to 50 DEG C, adding glucoamylase for saccharification, heating to 85DEG C after saccharification is carried out, and maintaining for 10 minutes; blending and inoculating: weighing 1000g of obtained millet powder, adding 4000mL of water, adding saccharification liquid of which the water volume is 10-20%, and inoculating lactobacillus fermentium, lactobacillus reuteri, lactobacillus plantarum and bifidobacterium adolescentis at the ratio of 3:1:2:1; fermentation: sealing a fermentation container, carrying out fermentation for 90 days under the condition of 28-35 DEG C, and carrying out after-ripening fermentation for 30 days under the condition of 4-10 DEG C, thus obtaining the pure natural lactobacillus millet enzyme after fermentation ends. No food additive is added into the pure natural lactobacillus millet enzyme, and the pure nature and the safety of food can be guaranteed furthest.

The invention belongs to the field of microecological preparations containing Chinese herbal medicines, and discloses forage clostridium butyricum, a microecological preparation with a function of promoting livestock and poultry growth and a preparation method of the microecological preparation. The preservation number of the clostridium butyricum RCM-2 is CCTCC NO: M2017396. A microecological preparation product prepared from the clostridium butyricum RCM-2 through fermentation comprises substrates in percentage by mass as follows: 25%-30% of powdery radix pseudostellariae rootlet extract, 15%-20% of fructus crataegi, 15%-20% of pericarpium citri reticulatae, 15%-20% of medicated leaven, 10%-15% of folium perillae, 5%-10% of chicory root, 10%-20% of green tea and 0.05%-0.1% of allicin. The clostridium butyricum RCM-2 has good resistance to tea polyphenol and allicin, and besides, the powdery radix pseudostellariae rootlet extract can remarkably promote growth of the strain. The microecological preparation not only is beneficial to preservation of effective constituents of a finished product, but also can obviously reduce damage rate of goods and prolong shelf time.

The invention belongs to the technical field of fermentation of probiotics, and relates to a solid state fermentation using bean pulp, bran and corn flour as matrixes and probiotics Lactobacillus casei HM-09 and Bacillus licheniformis BL-09 as strains, particularly a probiotic solid fermentation technique which can be widely used in the field of microecology. The method can be used for producing a fermented feed product with special sourness, and the fermented product has the advantages of high viable count and high degradation ratio of bean pulp proteins. The preparation method is low in production cost, can enhance the economic benefit and is simple to operate.

The invention discloses a natural astaxanthin-containing premix compound which comprises the following components according to weight ratio: 30-80g/kg of broken wall haematococcus pluvialis powder, 150-300g/kg of bean pulp, 150-200g/kg of calcium hydrophosphate, 150-250g/kg of mountain flour, 50-70g/kg of table salt, 0.6-0.9g/kg of nicotinic acid, 0.1-0.2g/kg of D-calcium pantothenate, 0.0016-0.0024g/kg of biotin, 7.5-12.5g/kg of choline chloride, 0.256-0.512g/kg of copper salt based on the content of copper, 1-2g/kg of ferric salt based on the content of ferrum, 1.2-2.4g/kg of zinc salt based on the content of zinc, 1.3-2.6g/kg of manganese salt based on the content of manganese, 0.02-0.04g/kg of iodate salt based on the content of iodate, 0.003-0.007g/kg of selenium salt based on the content of selenium, 20-35g/kg of methionine, 0.33-1.0g/kg of ethoxy quinoline, 1-3g/kg of phytase, 5-15g/kg of composite microecologics, 1-3g/kg of xylo-oligosaccharide and 80-120g/kg of bentonite. The natural astaxanthin-containing premix compound can remarkably improve the laying rate, reduce mortality and elimination rate, ensure proper colors of red-yolk eggs, promote the feed conversion rate, improve the production performance of laying hens and the eggshell quality, reduce the occurrence of diseases, and improve the economic efficiency.

The invention provides enterococcus faecium, a high-density fermentation method of enterococcus faecium, and a microecological preparation prepared by adopting enterococcus faecium, belonging to the technical field of culturing of lactic acid bacteria. An enterococcus faecium strain with the obvious probiotic property and stress resistance is screened and taken as a feed additive, and the enterococcus faecium strain can effectively replace part of antibiotics. The enterococcus faecium provided by the technical scheme is E13-5, and is assigned with the preservation number of CCTCC M2017191. Theenterococcus faecium provided by the invention can be used as the safe and efficient novel feed additive for replacing part of the antibiotics, and thus the demands of the modern feed industry and livestock breeding industry are satisfied.

The invention provides pediococcus acidilactici separated from Tibetan kefir and application of the pediococcus acidilactici in prevention and treatment of rotavirus infection, the pediococcus acidilactici separated from the Tibetan kefir is named as pediococcus acidilactici Wang, the pediococcus acidilactici is preserved in the China Center for Type Culture Collection on October 28, 2021, and the preservation number is CCTCC NO: M 20211331. The pediococcus acidilactici is resistant to acid and cholate, has a good inhibiting effect on most pathogenic bacteria, is sensitive to antibiotics, and shows an excellent rotavirus resisting effect in in-vivo and in-vitro experiments.